April 2002
02/9206/E PAD WCTR

Environmental Technoiogy
Verification Protocol

Drinking Water Systems Center

PROTOCOL FOR EQUIPMENT
VERIFICATION TESTING FOR PHYSICAL
CHEMICAL AND BIOLOGICAL
REMOVAL OF NITRATE

Prepared by

ฎ

NSF International

Under a Cooperative Agreement with

U.S. Environmental Protection Agency

eiVetVeiV


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EPA/NSF ETV

PROTOCOL FOR EQUIPMENT VERIFICATION TESTING
FOR PHYSICAL CHEMICAL AND BIOLOGICAL
REMOVAL OF NITRATE

Prepared by:
NSF International
789 Dixboro Road
Ann Arbor, MI 48105

Recommended by
the Steering Committee for the Verification of
Drinking Water Systems
on October 5, 1999
Modified on July 28, 2000 and in March 2002

With support from
the U.S. Environmental Protection Agency
Environmental Technology Verification Program

Copyright 2002 NSF International 40CFR35.6450.

Permission is hereby granted to reproduce all or part of this work, subject to
the limitation that users may not sell all or any part of the work and may not
create any derivative work therefrom. Contact ETV Drinking Water Systems
Center Manager at (800) NSF-MARK with any questions regarding authorized
or unauthorized uses of this work.


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NSF INTERNATIONAL

Mission Statement:

NSF International (NSF), an independent, not-for-profit organization, is dedicated to public health safety
and protection of the environment by developing standards, by providing education and providing superior
third party conformity assessment services while representing the interests of all stakeholders.

NSF Purpose and Organization

NSF International (NSF) is an independent not-for-profit organization. For more than 52 years, NSF has
been in the business of developing consensus standards that promote and protect public health and the
environment and providing testing and certification services to ensure manufacturers and users alike that
products meet those standards. Today, millions of products bear the NSF Name, Logo and/or Mark,
symbols upon which the public can rely for assurance that equipment and products meet strict public health
and performance criteria and standards.

Limitations of use of NSF Documents

This protocol is subject to revision; contact NSF to confirm this revision is current. The testing against this
protocol does not constitute an NSF Certification of the product tested.

U.S. ENVIRONMENTAL PROTECTION AGENCY

Throughout its history, the U.S. Environmental Protection Agency (EPA) has evaluated technologies to
determine their effectiveness in preventing, controlling, and cleaning up pollution. EPA is now expanding
these efforts by instituting a new program, the Environmental Technology Verification Program—orETV—
to verily the performance of a larger universe of innovative technical solutions to problems that threaten
human health or the environment. ETV was created to substantially accelerate the entrance of new
environmental technologies into the domestic and international marketplace. It supplies technology buyers
and developers, consulting engineers, states, and U.S. EPA regions with high quality data on the
performance of new technologies. This encourages more rapid availability of approaches to better protect
the environment.

ETV's Drinking Water Systems Center:

Concern about drinking water safety has accelerated in recent years due to much publicized outbreaks of
waterborne disease and information linking ingestion of arsenic to cancer incidence. The U.S. EPA is
authorized through the Safe Drinking Water Act to set numerical contaminant standards and treatment and
monitoring requirements that will ensure the safety of public water supplies. However, small communities
are often poorly equipped to comply with all of the requirements; less costly package treatment technologies
may offer a solution. These package plants can be designed to deal with specific problems of a particular
community; additionally, they may be installed on site more efficiently—requiring less start-up capital and
time than traditionally constructed water treatment plants. The opportunity for the sales of such systems in
other countries is also substantial.

The EPA has partnered with NSF, a nonprofit testing and certification organization, to verily performance of

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small drinking water systems that serve small communities. It is expected that both the domestic and
international markets for such systems are substantial. The EPA and NSF have formed an oversight
stakeholders group composed of buyers, sellers, and states (issuers of permits), to assist in formulating
consensus testing protocols. A goal of verification testing is to enhance and facilitate the acceptance of small
drinking water treatment equipment by state drinking water regulatory officials and consulting engineers
while reducing the need for testing of equipment at each location where the equipment use is contemplated.
NSF will meet this goal by working with equipment manufacturers and other agencies in planning and
conducting equipment verification testing, evaluating data generated by such testing, and managing and
disseminating information. The manufacturer is expected to secure the appropriate resources to support
their part of the equipment verification process, including provision of equipment and technical support.

The verification process established by the EPA and NSF is intended to serve as a template for conducting
water treatment verification tests that will generate high quality data for verification of equipment
performance. The verification process is a model process that can help in moving small drinking water
equipment into routine use more quickly. The verification of an equipment's performance involves five
sequential steps:

1.	Development of a verification/Product- Specific Test Plan;

2.	Execution of verification testing;

3.	Data reduction, analysis, and reporting;

4.	Performance and cost (labor, chemicals, energy) verification;

5.	Report preparation and information transfer.

This verification testing program is being conducted by NSF International with participation of
manufacturers, under the sponsorship of the EPA Office of Research and Development (ORD), National
Risk Management Research Laboratory, Water Supply and Water Resources Division (WSWRD) -
Cincinnati, Ohio. NSF's role is to provide technical and administrative leadership and support in conducting
the testing. It is important to note that verification of the equipment does not mean that the equipment is
"certified" by NSF or EPA. Rather, it recognizes that the performance of the equipment has been
determined and verified by these organizations.

Partnerships:

The U.S. EPA and NSF International (NSF) cooperatively organized and developed the ETV Drinking
Water Systems Center to meet community and commercial needs. NSF and the Association of State
Drinking Water Administrators have an understanding to assist each other in promoting and communicating
the benefits and results of the project.

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ORGANIZATION AND INTENDED USE OF PROTOCOL AND TEST PLANS

NSF encourages the user of this protocol to also read and understand the policies related to the verification
and testing of drinking water treatment systems and equipment.

The first Chapter of this document describes the Protocol required in all studies verifying the performance of
equipment or systems removing nitrate, the public health goal of the Protocol. The remaining chapters
describe the additional requirements for equipment and systems using specific technologies to attain the
goals and objectives of the Protocol: the removal of nitrate.

Prior to the verification testing of drinking water treatment systems, plants and/or equipment, the equipment
manufacturer and/or supplier must select an NSF-qualified Field Testing Organization (FTO). This
designated Field testing Organization must write a "Product-Specific Test Plan" (PSTP). The equipment
manufacturer and/or supplier will need this protocol and the test plans herein and other ETV Protocols and
Test Plans to develop the Product- Specific Test Plan depending on the treatment technologies used in the
unit processes or treatment train of the equipment or system. More than one protocol and/or test plan may
be necessary to address the equipment's capabilities in the treatment of drinking water.

Testing shall be conducted by an NSF-qualified Field Testing Organization that is selected by the
Manufacturer. Water quality analytical work to be completed as a part of an ETV Testing Plan shall be
contracted with a state-certified or third party- or EPA-accredited laboratory. For information on a listing
of NSF-qualified field testing organizations and state-certified or third party- or EPA-accredited
laboratories, contact NSF International.

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ACKNOWLEDGMENTS

The U.S. EPA and NSF would like to acknowledge those persons who participated in the preparation,
review and approval of this Protocol. Without their hard work and dedication to the project, this document
would not have been approved through the process which has been set forth for this ETV project.

Chapter 1: Requirements for All Studies

Writers: Gerald Guter, Guter Consulting and Steven Duranceau, Boyle Engineering
Technical reviewers: Dennis Clifford, University of Houston and Tom Sorg, US EPA

Chapter 2: Test Plan for Reverse Osmosis and Nanofiltration

Writers: Jim Lozier and Paul Mueller, CH2M Hill
Technical reviewer: James Taylor, University of Central Florida

Chapter 3: Test Plan for Ion Exchange

Writers: Gerald Guter, Guter Consulting and Steven Duranceau, Boyle Engineering
Technical reviewers: Dennis Clifford, University of Houston and Tom Sorg, US EPA

Chapter 4: Test Plan for Heterotrophic Biological Denitrification

Writer: Dr. Mohamed F. Dahab, University of Nebraska, Lincoln
Technical reviewer: Dr. Bruce Rittmann, Northwestern University

Steering Committee Members that voted on Chapters 1 through 3:

Mr. Jim Bell	Dr. Gary S. Logsdon

Mr. Jerry Biberstine, Chairperson	Mr. Bob Mann

Mr. John Dyson	Mr. David Pearson

Mr. Allen Hammer	Mr. Peter Shanaghan

Dr. Joseph G. Jacangelo	Mr. John Trax
Mr. Glen Latimer

Steering Committee Members that voted on Chapter 4:

Mr. Jim Bell	Mr. Glen Latimer

Mr. Kevin Brown, Chairperson	Dr. Gary S. Logsdon

Mr. Stephen Clark	Mr. Bob Mann

Mr. John D. Dyson	Mr. David Pearson

Mr. Joe Harrison	Mr. Ed Urheim

Dr. Joseph G. Jacangelo	Mr. Victor Wilford

Page v

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TABLE OF CONTENTS

Page

Chapter 1: EPA/NSF ETV Protocol for Equipment Verification Testing for Removal of
Nitrate: Requirements for All Studies	1-1

Chapter 2: EPA/NSF ETV Equipment Verification Testing Plan - Removal of Nitrate
by Reverse Osmosis and Nanofiltration	2-1

Chapter 3: EPA/NSF ETV Equipment Verification Testing Plan - Nitrate Contaminant
Removal by Ion Exchange	3-1

Chapter 4: EPA/NSF ETV Equipment Verification Testing Plan - Heterotrophic
Biological Denitrification for the Removal of Nitrate	4-1

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CHAPTER 1

EPA/NSF ETV PROTOCOL FOR EQUIPMENT VERIFICATION TESTING

FOR REMOVAL OF NITRATE

REQUIREMENTS FOR ALL STUDIES

Prepared by:
NSF International
789 Dixboro Road
Ann Arbor, MI 48105

Copyright 2002 NSF International 40CFR35.6450.

Permission is hereby granted to reproduce all or part of this work, subject to
the limitation that users may not sell all or any part of the work and may not
create any derivative work therefrom. Contact ETV Drinking Water Systems
Center Manager at (800) NSF-MARK with any questions regarding authorized
or unauthorized uses of this work.

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TABLE OF CONTENTS

Page

1.0	INTRODUCTION	1-5

1.1	Background	1-8

1.2	Objectives of Verification Testing	1-8

1.3	Scope of Verification Process and Testing	1-9

1.4	Scope of the PSTP	1-9

1.5	General Content of PSTP	1-10

2.0	EQUIPMENT VERIFICATION TESTING RESPONSIBILITIES	1-11

2.1	Verification Testing Organization and Participants	1-11

2.2	Organization	1-11

2.3	Verification Testing Site Name and Location	1-11

2.4	Site Characteristics	1-12

3.0	EQUIPMENT CAPABILITIES AND DESCRIPTION	1-13

3.1	Equipment Capabilities, Water Quality Obj ectives	1-13

3.2	Equipment Description	1-14

4.0	EXPERIMENTAL DESIGN	1-17

4.1	Obj ectives of Experimental Testing	1-17

4.2	Equipment Characteristics or Factors to be Tested	1-17

4.2.1	Qualitative F actors	1-17

4.2.2	Quantitative F actors	1-18

4.2.3	Quantitative F actors: Definitions	1-18

4.2.4	Quantitative Cost Factors	1-19

4.2.5	Quantitative Plant Performance Factors	1-20

4.2.6	Quantitative Factors, Plant Health, Safety and Reliability	1-21

4.2.7	Cross Connection Control References and Guidelines	1-23

4.3	Water Quality Considerations	1-23

4.3.1	F eed W ater Quality	1-24

4.3.2	Treated Water Quality	1-24

4.3.3	Wastewater Characteristics, Quality and Quantity	1-25

4.4	Recording Water Quality Data	1-25

4.5	Recording Statistical Uncertainty	1-26

4.6	Instrumentation for Plant Control and Monitoring	1-27

4.6.1	Nitrate Measurements	1-27

4.6.2	Electrical Conductivity Measurements	1-27

4.7	Verification Testing Schedule	1-28

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TABLE OF CONTENTS (continued)

Page

5.0	FIELD OPERATIONS PROCEDURES	1-29

5.1	Equipment Operations and Design	1-29

5.2	Communications, Documentation, Logistics, and Equipment	1 -29

5.3	Initial Operations	1-30

5.4	Equipment Operation and Water Quality Sampling for Verification Testing	1-30

6.0	QUALITY ASSURANCE PROJECT PLAN (QAPP)	1-31

6.1	Purpose and Scope	1-31

6.2	Quality Assurance Responsibilities	1-31

6.3	Data Quality Indicators	1-32

6.3.1	Accuracy	1-32

6.3.2	Precision	1-33

6.3.3	Representativeness	1-34

6.3.4	Statistical Uncertainty	1-34

6.4	Quality Control Checks	1-34

6.4.1	Quality Control for Equipment Operation	1-35

6.4.2	Water Quality Data	1-35

6.4.2.1	Duplicate Samples	1-35

6.4.2.2	Method Blanks	1-35

6.4.2.3	Spiked Samples	1-35

6.4.2.4	Travel Blanks	1-35

6.4.2.5	Performance Evaluation Samples for On-Site Water Quality

Testing	1-35

6.5	Data Reduction, Validation, and Reporting	1-36

6.5.1	Data Reduction	1-36

6.5.2	Data Validation	1-36

6.5.3	Data Reporting	1-36

6.6	System Inspections	1-37

6.7	Reports	1-37

6.7.1	Status Reports	1-37

6.7.2	Inspection Reports	1-37

6.8	Corrective Action	1-37

7.0	DATA MANAGEMENT AND ANALYSIS, AND REPORTING	1-38

7.1	Data Management and Analysis	1-38

7.2	Report of Equipment Testing	1-39

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TABLE OF CONTENTS (continued)

Page

8.0 SAFETY MEASURES	1-39

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1.0 INTRODUCTION

This first chapter is the "EPA/NSF ETV Protocol For Equipment Verification Testing For Removal Of
Nitrate: Requirements For All Studies". Specifically, this protocol discusses the information and procedures
requested from equipment manufacturers who wish to have their treatment plants verified and tested under
the NSF International/Environmental Protection Agency (NSF/EPA) verification testing program. In order
to participate in the equipment verification process a Product-Specific Test Plan (PSTP) using this study
protocol and adhering to the requirements herein is necessary.

The contents of the PSTP are described in this protocol document. The manufacturer will include only
those items of information that pertain to the specific equipment and testing objectives. The descriptive
material in this protocol represents the format and type of information, which would be required for
NSF/EPA ETV testing. The PSTP should not be viewed as a promotional document, but as a document
which will transfer technical information about the equipment, the site of the testing and information regarding
successful operation to those unfamiliar with the equipment and location of the test.

The testing of new technologies and materials that are unfamiliar to the NSF/EPA will not be discouraged.
It is recommended that resins or membranes or any other material or chemical in the equipment conform to
NSF International/American National Standards Institute (NSF/ANSI) Standard 60 and 61. The
disclosure of the existence or use of proprietary or patented material and procedures should be made in the
PSTP.

The final submission of the PSTP shall:

•	include the information requested in this protocol;

•	conform to the format identified herein;

•	and conform to the specific ETV Testing Plan or Plans related to the statement or statements of
capabilities that are to be verified.

The PSTP may include more than one Testing Plan. For example, testing might be undertaken to verily
performance of a system for both nitrate reduction and removal of disinfection by-product precursors.

This protocol document is presented in two fonts. The non-italicized font provides background information
that the Field Testing Organization (FTO) may find useful in preparation of the PSTP. The italicized text
indicates specific study protocol deliverables that are required of the Field Testing Organization and
Manufacturer and that must be incorporated in the PSTP.

The following glossary terms are presented here for subsequent reference in this protocol:

•	Distribution System - A system of conduits by which a primary water supply is conveyed to
consumers typically by a network of pipelines.

•	EIR - An Environmental Impact Report that may be required to construct and operate a drinking water
treatment plant.

•	Equipment Verification Testing Plan - specific testing plan for each technology application, such as

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nitrate equipment or coagulation and filtration equipment. These plans are being developed by NSF to
assist in development of PSTPs.

•	Field Testing Organization (FTO) - an organization qualified to perform studies and testing of
package plants or modular systems. The role of the testing organization is to ensure that there is skilled
operation of a system during the intense periods of testing during the study and the tasks required by the
Protocol for Equipment Verification Testing are performed. The Testing Organization is responsible for:

•	preparing the application on behalf of the Manufacturer;

•	managing, evaluating, interpreting and reporting on the data produced by the verification testing and
study;

•	providing logistical support, scheduling and coordinating the activities, e.g., establishing a
communications network, of all participants in the verification testing and study;

•	advising the Manufacturer on feed water quality, waste disposal requirements and test
site selection, such that locations selected for the verification testing and study have feed
water quality consistent with the objectives of the Protocol for Equipment Verification
Testing;

•	collecting and transporting analytical samples of water from water and wastewater
streams and maintaining chain of custody documents;

•	collecting all field data on qualitative and quantitative evaluation factors.

•	Manufacturer - A business that assembles and/or sells package plant equipment and/or modular
systems. The role of the manufacturer is to provide the package plant and technical support for the
verification testing and study. The manufacturer is also responsible for providing assistance to the
testing organization during operation and monitoring of the package plant or modular system during the
verification testing and study.

•	Modular System - A functional assembly of components for use in a drinking water treatment system
or package plant, each part of which provides a limited form of treatment of the feed water(s). Treated
waters may be discharged to another package plant module or to the distribution system if the modular
system includes the final step of treatment.

•	Package Plant - A complete water treatment system including all components from connection to the
feed water(s) through discharge to the distribution system. It is the entire system of water treatment
plant equipment that is provided by the manufacturer. It shall include all equipment and materials which
an owner/operator requires or is required by permits to install the system, operate it and discharge the
final product into the distribution system, and to discharge waste into a waste disposal system. Any
post treatment or blending facilities are included in the package plant. The package plant does not
include any existing source water facilities, but it may or may not include waste discharge or containment
facilities.

•	Performance - Various plant operating factors described either quantitatively or qualitatively which
characterize the plant's ability to meet the objectives of the treatment process.

•	Permit - Any permit that is required to install and operate a drinking water treatment plant such as

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conditional use permit, a construction permit, a treatment plant operation permit, a waste discharge
permit or other such permit.

•	Plant Operator - the person working for a small water system who is certified to operate a water
treatment plant and who is responsible for operating drinking water treatment equipment to produce
treated drinking water. This person also may collect samples, record data and attend to the daily
operations of equipment throughout the testing periods.

•	Product-Specific Test Plan (PSTP) - A written document of procedures for on-site/in-line testing,
sample collection, preservation, and shipment and other on-site activities described in the EPA/NSF
Protocol(s) and Test Plan(s) that apply to a specific make and model of a package plant/modular
system.

•	Protocol for Equipment Verification Testing - this document. Protocol shall be used for reference
during Manufacturer participation in verification testing program

•	Reclaimed Water - Water that is a by-product of the treatment process and is discharged as a
wastewater and is reused as irrigation water, cooling tower water or to satisfy similar water
demands.

•	Recycled Water - Water that is a byproduct of the treatment process that is reused in the
process rather than being discharged as wastewater.

•	Reliability - The ability of a system to meet the obj ectives of the treatment process over a long term on
a consistent basis without excessive maintenance, operator time and down time.

•	Responsible Water Agency or Owner/Operator - The person or agency (private or public) that
owns the site and facilities where the system will be tested. It is likely that the owner will already be
operating some water system facilities, such as a well, pump station, reservoir, treatment plant etc. at
this site. This agency also represents a typical end user or purchaser of a system who has public water
purveyor responsibilities under numerous local, State and Federal regulations.

•	Verification - to establish the evidence on the range of performance of equipment and/or device under
specific conditions following a predetermined protocol.

•	Waste, Waste Solids, Wastewater - The solid, liquid, or mixture of solid and liquid material that is
produced by the water treatment processing equipment consisting of concentrated nitrate and other salt
brines and rinse water and backwash water.

•	Waste System - The portion of the equipment that contains, stores, transports, or pumps the
produced waste.

•	Waste Disposal System - A facility, such as a sewer system, irrigated area, waste disposal site,
ocean outfall, evaporation pond, deep well disposal system or other such system which will accept the
waste produced by the equipment. This waste disposal system is not a part of the equipment.

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1.1 Background

The U.S. Environmental Protection Agency (EPA) has partnered with NSF, a not-for-profit testing and
certification organization, to verify performance of small drinking water systems that serve small
communities. It is expected that both the domestic and international markets for such systems are
substantial. EPA and NSF have formed an oversight stakeholders group composed of buyers, sellers,
consultants, organizations and states (issuers of permits), to assist in formulating consensus testing protocols.
A goal of verification testing is to enhance and facilitate the acceptance of small drinking water treatment
equipment by State drinking water regulatory engineers and consulting engineers while reducing the need for
testing of equipment at each location where the equipment use is contemplated.

NSF will meet this goal by working with equipment manufacturers and other agencies in planning and
conducting equipment verification testing, evaluating data generated by such testing and managing and
disseminating information. The manufacturer is expected to secure the appropriate resources to support
their part of the equipment verification process, including provision of equipment and technical support.

The verification process established by the EPA and NSF is intended to serve as a template for conducting
water treatment verification tests that will generate high quality data for verification of equipment
performance. The verification process is a model process that can help in moving small drinking water
equipment into routine use more quickly. The verification of an equipment's performance involves five
sequential steps:

•	Development of a verification/ PSTP;

•	Execution of verification testing;

•	Data reduction, analysis, and reporting;

•	Performance, reliability and cost factor (labor, chemicals, energy) verification;

•	Report preparation and information transfer.

1.2 Obj ectives of Verification Testing

The manufacturer will define the verification testing objective(s). These specific objectives of the equipment
verification testing will be different for each Manufacturer, depending upon the statement of objectives of the
specific equipment to be tested. The testing objectives developed by each Manufacturer shall be defined
and described in detail in the PSTP developed for each piece of equipment. The objectives of the
equipment verification testing may include:

•	Generate field data appropriate for verifying the performance of the equipment;

•	Evaluate new advances in equipment and equipment design;

•	Generate field data appropriate for verifying the performance of the equipment used in a specific
environment such as a coastal region where ocean disposal is available;

•	Generate field data appropriate for verifying the performance of the equipment operating within a

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specific range of untreated water quality;

•	Generate field data appropriate for verifying the performance of the equipment used for specific
modes of operation such as continuous or interrupted operation.

Multiple testing objectives may be included in the PSTP. The development of specific objectives is
discussed in Section 4.1. Water quality treatment objectives must also be defined in the PSTP. The
development of these objectives is discussed in Section 3.1.

An important aspect in the development of the verification testing is to describe the procedures that will be
used to verify the statement of performance objectives made for water treatment equipment. A verification
testing plan document incorporates the quality assurance/quality control (QA/QC) elements needed to
provide data of appropriate quality sufficient to reach a defensible position regarding the equipment
performance. Verification testing conducted at a single site may not represent every environmental situation
which may be acceptable for the equipment tested, but it will provide data of sufficient quality to make a
judgment about the application of the equipment under conditions similar to those encountered in the
verification testing.

It is important to note that verification of the equipment does not mean that the equipment is "certified" by
NSF or EPA. Rather, it recognizes that the performance of the equipment has been determined and verified
by these organizations.

1.3	Scope of Verification Process and Testing

This protocol outlines the verification process for equipment designed to achieve the physical chemical or
biological removal of nitrate from contaminated water. The scope of this protocol includes Testing Plans for
equipment employing ion exchange, reverse osmosis, electrodialysis, or biological processes as the primary
process for treatment. The equipment may consist of a single process or a combination of these processes.
The equipment may also consist of one or more of these processes combined with a waste treatment
process, such as biological denitrification. This protocol is not an NSF or third-party consensus standard
and it does not endorse the products or technology described herein. An overview of the equipment
verification process and the elements are described in this protocol document.

1.4	Scope of the PSTP

Specifically, the PSTP shall include at least the following items:

•	Roles and responsibilities of verification testing participants; (See Section 2.0)

•	A brief statement of the objectives of the test plan;

•	A brief statement of the water quality treatment objectives;

•	Procedures governing verification testing activities such as equipment operation and process
monitoring; sample collection, preservation, and analysis; and data collection and interpretation; (See
Section 3.0)

•	Experimental design of the Field Operations Procedures; (See Sections 4.0 and 5.0)

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•	Quality assurance (QA) and quality control (QC) procedures for conducting the verification testing
and for assessing the quality of the data generated from the verification testing; (See Sections 6.0 and
7.0) and

•	Health and safety measures relating to biohazard (if present), chemical, electrical, mechanical and
other safety codes. (See Section 8.0)

1.5 General Content of PSTP:

The remaining sections of this chapter discuss the type of information, which is required in the PSTP. At the
end of each section the italicized textual material will give the required outline for treating the subjects
discussed in the related section followed by a statement of the responsibilities of the participants. For
example, the following is the general outline for the PSTP:

The structure of the PSTP must conform to the outline below: The required components of the
Document shall be described in greater detail in the sections below.

•	TITLE PAGE

•	FOREWORD

•	TABLE OF CONTENTS -The Table of Contents for the PSTP shall include the headings
provided in this document although they may be modified as appropriate for a particular type
of equipment to be tested.

•	EXECUTIVE SUMMARY-The Executive Summary describes the contents of the PSTP (not to
exceed two pages). A general description of the equipment, the testing objectives, and the
statement of water quality treatment objectives and capabilities which shall be verified during
testing shall be included, as well as the testing locations, a schedule, and a list of participants.

•	ABBREVIATIONS AND ACRONYMS - A list of the abbreviations and acronyms used in the
PSTP shall be provided.

•	EQUIPMENT VERIFICATION TESTING RESPONSIBILITIES (See Section 2, below.)

•	EQUIPMENT CAPABILITIES AND DESCRIPTION (See Section 3, below.)

•	EXPERIMENTAL DESIGN (See Section 4, below.)

•	FIELD OPERATIONS PROCEDURES (See Section 5, below.)

•	QUALITY ASSURANCE TESTING PLAN (See Section 6, below.)

•	DA TA MANAGEMENT AND ANALYSIS (See Section 7, below.)

•	SAFETY PLAN (See Section 8, below.)

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2.0 EQUIPMENT VERIFICATION TESTING RESPONSIBILITIES

2.1	Verification Testing Organization and Participants

This verification testing program is being conducted by NSF International with participation of
manufacturers, under the sponsorship of the EPA Office of Research and Development, National Risk
Management Research Laboratory, Water Supply and Water Resources Division (WSWRD)- Cincinnati,
Ohio. The WSWRD and NSF jointly are administering the Equipment Verification Testing Program.
NSF's role is to provide technical and administrative leadership and support in conducting the testing.

The specific responsibilities of each participant are discussed in Section 2.6. The required content of the
PSTP and the Manufacturer responsibilities are listed at the end of each section. In the development of a
PSTP, a table that includes the name, affiliation, and mailing address of each participant, a point of contact,
their role, and telephone, fax and E-mail address shall be provided.

The following participants should be listed in the Participants Table:

•	NSF;

•	Site Owner;

•	Site Operator;

•	Plant Operator;

•	Field Testing Organization;

•	Analytical Laboratory; and

•	Any other responsible parties.

2.2	Organization

The PSTP shall provide the organizational structure for the verification testing showing lines of
communication.

2.3	Verification Testing Site Name and Location

This section discusses background information on the verification testing site(s), with emphasis on the quality
of the feed water, which in most cases will be the source water at the site. The need for treatment should be
demonstrated. The name and address of the owner/operator of the proposed site must be submitted along
with a letter of agreement from the owner/operator of the site to allow testing of the equipment at the site.
The Manufacturer should consider listing a site, which would be typical of the intended market not only in
terms of the feed water quality but also in terms of location and environmental conditions. For example, if a
manufacturer's market is in mid-western agricultural regions, the water quality, climate, and waste disposal
systems would be different than those found in temperate coastal regions. If the manufacturer wishes to
demonstrate a membrane system, choice of a site with total dissolved solids (TDS) water quality problems
would be desirable. It is likely that the equipment would be tested at a well site owned and operated by a

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public water agency or private water company. In most cases, the equipment may be demonstrated at
more than one site.

2.4 Site Characteristics

The PSTP must include a description of the test site and the immediately surrounding environment.
Information about the site should be provided to show that it is feasible to adequately test a system(s). This
shall include a description of where the equipment will be located. If available, give the street address, city,
state and zip code. An area location map showing access from major streets and highways and a site layout
drawing with equipment foot prints and dimensions would be helpful. The drawing should indicate the
location of existing facilities, the source of the feed water and where the treated water will go and where the
waste will be discharged. It should be specifically mentioned if the treated water will proceed to waste or if
it will be introduced into an existing water supply. It is also important to point out if treated water will be
blended with untreated water before it is sent to waste or distribution. If so, the blending facility must be a
part of the equipment. Indicate if any facilities other than the equipment would be required such as
additional buildings or trailers for sample collection and analyses, electrical power, concrete pads, drainage
facilities, protective coverings etc. Consider the following questions:

•	Will the equipment supply water on a continuous or interrupted basis?

•	If the equipment is down, how will the demand be met?

•	If the feed water is the source water for an existing water treatment plant, describe the raw water
intake, the opportunity to obtain raw water without the addition of any chemicals as feed water to
the equipment being tested, the pattern of operation of the raw water pumping (is it continuous or
intermittent), if source water available from a pressurized line or from a storage reservoir, and
facilities for handling treated water and waste (i.e., residuals) from the testing program.

•	Do facilities exist on the site for disposal of wastes? If so, is the capacity large enough to handle
waste from the equipment? Or, will new waste disposal facilities be required? The equipment will
be tested at its maximum sustainable treatment flow rate (gallons per minute).

•	What is this value and demonstrate it is compatible with the water supply and demand at the site?

•	Will the operation of the test facility be compatible with the existing uses of the site and the
surrounding land uses?

•	Is it located in a residential area where neighbors may complain? If so, how will this be handled?

•	Are any construction or conditional use permits required? Is a permit required to operate the
equipment?

•	Is a waste discharge permit required?

•	What will be the ultimate method of waste disposal?

•	Has the manufacturer consulted with the following: The owner/operator, the water agency, the local
or State health organization, the waste discharge agency, local emergency officials (regarding safe
fire flows), or responsible health agencies who have jurisdiction?

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•	Have alternative sites been considered?

•	Why was this site chosen in preference to other sites?

•	Environmental documentation such as an EIR or environmental impact assessment relative to the
proposed equipment testing should also be submitted or an explanation of why such environmental
documentation may or may not be required.

Content of PSTP Regarding Equipment Verification Testing Responsibilities:

The Field Testing Organization shall be responsible for including the following elements in the
PSTP:

•	Definition of the roles and responsibilities of appropriate verification testing participants.

•	A table which includes the name, affiliation, and mailing address of each participant, a point
of contact, their role, telephone, fax and E-mail address.

•	Organization of operational and analytical support.

•	List of the site name(s) and location(s). Describe the site and the surroundings. Use location
maps and provide other material discussed above.

•	Description of the test site(s), the site characteristics and identification of where the
equipment shall be located (use foot print drawings) and other pertinent material discussed
above.

•	Describe existing facilities on the site, how they will be used. List any permits and
environmental documentation required. Provide other material discussed above.

Manufacturer Responsibilities:

•	Provision of complete, field-ready equipment for verification testing.

•	Provision of logistical and technical support, as required. Remove equipment and any
discarded items from the site after termination of the test program.

•	Provision of technical assistance to the site owner/operator and the qualified testing
organization during operation and monitoring of the equipment undergoing verification
testing as discussed above.

3.0	EQUIPMENT CAPABILITIES AND DESCRIPTION

3.1	Equipment Capabilities, Water Quality Objectives

For this Verification Testing, the Manufacturer and their designated FTO shall identify in a Statement of
Performance Objectives the specific performance criteria to be verified and the specific operational
conditions under which the Verification Testing shall be performed. The manufacturer's performance
objectives are used to establish data quality objectives (DQOs) in order to develop the experimental design
of the verification test. The broader the performance objectives, the more comprehensive the PSTP must

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become to achieve the DQOs. In conjunction with a Statement of Performance Objectives, the FTO shall
state the pertinent detection limits for the nitrate analytical method. Statements should be made regarding
the applications of the equipment, the known limitations of the equipment and under what conditions the
equipment is likely to fail or underperform. The FTO on behalf of the Manufacturer shall also provide
information as to what advantages the Verification Testing equipment provides over existing equipment. The
PSTP must state the treated water quality objectives.

The Statement of Performance Objectives must be specified and verifiable by a statistical analysis of the
data. Below are two different types of Statements of Performance Objectives that may be verified in this
testing:

1.	This ion exchange system is capable of treating water contaminated with nitrate up to 100 mg
NO3/L by producing water with a nitrate level equal to or less than 35 mg NO3/L in the treated water
samples during a 60-day operation period at a loading rate of 1 gpm/cf of resin (temperature between 20
and 25ฐC).

2.	This system is capable of producing a product water with nitrate concentration less than 10 mg/L
during a 60-day operation period at a loading rate of 1 gpm/cf of resin (temperature between 20 and 25ฐC)
in feedwaters having the following composition: IDS = 1200 mg/L (with sulfates = 300 mg/L or greater)
and containing nitrate having a level of up to 100 mg NO3/L and will produce water with a IDS level equal
to 150 mg/L and a nitrate level equal to or less than 35 mg N03/L in the treated water samples.

Note in the above statement for membrane equipment, the sulfate level must also be stated.

An example of a Statement of Performance Objectives that would not be acceptable is presented below:

"This system will provide lower nitrate levels than required by the Safe Drinking Water Act
(SDWA) on a consistent and dependable basis."

The Manufacturer shall identify the water quality objectives to be achieved in the Statement of Performance
Objectives of the equipment to be evaluated in the verification testing. For each Statement of Performance
Objectives proposed by the FTO and the Manufacturer in the PSTP, the following information shall be
provided:

•	Applications of the equipment;

•	Known limitations of the equipment;

•	Advantages it provides over existing equipment;

•	Percent removal of nitrate;

•	Rate of treated water production (i.e., resin loading rate, membrane flux);

•	Product water recovery;

•	Feed stream water quality regarding pertinent water quality parameters;

•	Temperature;

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• Concentration of nitrate; and

Other pertinent water quality and operational conditions.

During Verification Testing, the FTO must demonstrate that the equipment is operating at a steady-state
prior to collection of data to be used in verification of the Statement of Performance Objectives. The
following equation shall be used to determine percent removal of the radionuclides investigated:

% Nitrate Removal =100*

C -C

feed	finished

C

feed

where: Cfeed = concentration of nitrate in the feedwater; and
Cfinished = concentration of nitrate in the finished water.

The analysis of nitrates in the feedwater, treated water and wastewater streams shall be performed by a
state-certified, third party accredited or EPA-accredited laboratory using an approved Standard Method.

The Statement of Performance Objectives prepared by the FTO (in collaboration with the Manufacturer)
shall also indicate the range of water quality under which the equipment can be challenged while successfully
treating the feedwater. Statements of Performance Obj ectives that are too easily met may not be of interest
to the potential user, while performance objectives that are overstated may not be achievable. If a
manufacturer relies on integrated processes for nitrate removal, the Statement of Performance Objectives
must include the overall water treatment system nitrate removal performance. The Statement of
Performance Objectives forms the basis of the entire Equipment Verification Testing Program and must be
chosen appropriately. Therefore, the design of the PSTP should include a sufficient range of feedwater
quality to permit verification of the Statement of Performance Objectives.

It should be noted that many of the drinking water treatment systems participating in the Nitrate Removal
Verification Testing Program will be capable of achieving multiple water treatment obj ectives. Although this
Protocol and the associated Verification Testing Plans are oriented towards removal of nitrates from
feedwaters, the Manufacturer may want to look at the treatment system's removal capabilities for additional
water quality parameters.

3.2 Equipment Description

Description of the equipment for the verification testing program shall be provided by the Manufacturer.
Data plates shall be permanent and securely attached to each production unit. The data plate shall be easy
to read in English or the language of the intended user located on the equipment where it is readily
accessible. Open/closed indicators shall be clearly visible on all automatic valves as well as direction of flow
arrows on all piping. The following other information is required"

a)	Equipment Name

b)	Model #

c)	Manufacturer's name and address

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d)	Electrical requirements - volts, amps, and Hertz

e)	Serial Number and Year of manufacture

f)	Warning and Caution statements in legible and easily discernible print size

g)	Capacity or output rate (if applicable)

h)	Any proprietary features should be described

Content of PSTP Regarding Equipment Capabilities and Description:

The PSTP shall contain the following:

•	A statement of the water quality objectives to be achieved by the equipment.

•	Description of the equipment to be demonstrated. This shall include:

-	Brief introduction and discussion of the engineering and scientific concepts on which the
water treatment equipment is based;

-	Description of the treatment train and each unit process included in the system;

-	Brief description of the physical construction/components of the equipment. Include
general environmental requirements and limitations, weight, transportability, ruggedness,
power and other consumables needed, etc.;

-	Statement of typical rates of consumption of chemicals, a description of the physical and
chemical nature of wastes, and rates of waste production concentrates, residues, etc.).

•	Definition of the performance range of the equipment.

•	Operation and maintenance manual will be supplied for the equipment. Include a flow diagram,
piping and instrumentation diagram, location of sampling points and flow meters, and a
description ofa typical start up, operation and shut down procedure. Indicate the functioning of
alarms and shut down alarms. Indicate plant adjustments required. All valves and controls and
similar components are to be clearly marked on the equipment and so identified in the flow
diagram.

•	Identification of any special licensing requirements associated with the operation of the
equipment.

•	Description of the applications of the equipment and the removal capabilities of the treatment
system relative to existing equipment. Provide comparisons in such areas as: treatment
capabilities, requirements for chemicals and materials, power, labor requirements, suitability for
process monitoring and operation from remote locations, ability to be managed by part-time
operators.

•	Discussion of the known limitations of the equipment. Include such items as the range of feed
water quality suitable for treatment with the equipment, the upper limits for concentrations of
regulated contaminants that can be removed to specified concentrations, level of operator skill
required to successfully use the equipment.

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4.0 EXPERIMENTAL DESIGN

This section discusses the objectives of the verification testing, factors that must be considered to meet the
performance objectives, and the statistical and other means that the NSF should use to evaluate the results
of the verification testing.

4.1	Objectives of Experimental Testing

The testing program must have well defined objectives. The PSTP will include a statement of the
verification testing objectives to evaluate equipment in one or more of the following areas:

1)	performance relative to manufacturer's stated range of equipment objectives;

2)	the impacts of variations in feed water quality (specifically variations in IDS, chloride, sulfate,
nitrate and alkalinity are important) on its performance;

3)	the logistical, human, and economic resources necessary to operate the equipment;

4)	the reliability, ruggedness, cost, range of usefulness, safety and ease of operation and maintenance;

5)	how much (or how little) waste is produced by the treatment process; and

6)	the cost of treatment.

Although the Field Testing Organization is encouraged to include all parameters listed below in the PSTP,
the Field Testing Organization shall be responsible for selection of the qualitative and quantitative parameters
which must be evaluated to meet the water quality treatment objectives and the verification testing
objectives. A list of parameters is listed below and in ETV test plans that are appropriate for most
equipment. For example, if equipment is only intended for removal of nitrate, there would be no need to
conduct testing to evaluate the removal of IDS. The Field Testing Organization will state the verification
testing objective that is appropriate for the equipment. For example, the Field Testing Organization may
wish to focus on wastewater production and thus formulate the stated objective to include this factor.

4.2	Equipment Characteristics or Factors to be Tested

This section discusses factors that shall be considered in the design and implementation of the verification
testing. These factors will be evaluated either quantitatively or qualitatively during the verification testing.
The factors can include such items as: ease of operation; ease of maintenance; degree of operator attention
required; operator labor (man-hours) required; response of equipment and treatment process to changes in
feed water quality; electrical power requirements; system reliability features including redundancy of
components; feed flow requirements; discharge requirements; spatial requirements for the equipment
(footprint); unit processes included in treatment train; and chemical inventories needed.

4.2.1 Qualitative Factors

Some factors, while important, are difficult or impossible to test or quantify. Important factors that
cannot easily be quantified are the modular nature of the equipment, the safety of the equipment toward
operators or spectators, the portability of equipment, and the logistical requirements necessary for using

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it. Aesthetics, security and compatibility with surrounding land use are important qualitative factors.
For example, a plant located h a residential area should not appear out of place and it should be
protected from and should not attract vandalism.

Maintenance operations such as ease/difficulty of membrane or resin cleaning and replacement should
be discussed although these operations may not be carried out during the test procedure. NSF would
also require knowing what labor would be required to terminate the tests and remove the equipment
from the site.

Typical qualitative factors to be discussed are listed below, and others may be added. The PSTP shall
discuss those factors that are appropriate to the test equipment and the test site.

•	Reliability or susceptibility to environmental conditions.

•	Equipment safety. Point out any feature or device which could harm the operator if
malfunctions or mishandling occurred, such as chemical handling, high pressures discharges,
unexpected noises, sudden pressure loss or build up.

•	The need for any safety devices (e.g. fire extinguishers, air packs) or clothing which are required
such as special shoes or safety glasses.

•	Effect of operator experience on results. Discuss the level of experience that an operator
should have to successfully achieve reliable and safe operation. Mention if special training
would be required such as handling acids, leaks or spills.

•	Special equipment required for moving or lifting heavy parts or materials.

•	Aesthetics and security.

•	Compatibility with surrounding land use.

•	Health and safety features.

•	Alarms and set points.

•	Placement of instrumentation and monitoring devices for convenient operator reading, inspection
and replacement.

4.2.2	Quantitative Factors

Many factors in this verification testing can be quantified by various means. Some can be measured and
controlled while others such as chemical market prices cannot be controlled but can be measured.
Typical quantitative factors to be considered are listed below, and others may be added. The PSTP
shall list and give estimates of these factors and describe how they can be measured during the field
operation of the test equipment. These factors will be also be field tested, measured and verified by the
Testing Organization during the testing and verification procedure.

4.2.3	Quantitative Factors: Definitions

The following definitions apply to the discussion of quantitative factors:

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•	Untreated Water - The raw water which is delivered or available at the site for treatment by the
equipment for nitrate removal.

•	Treated Water - The water stream that has passed through treatment (and post treatment) and is
available from the equipment either for direct injection into a distribution system or for blending with
untreated water before injection into the water supply system.

•	Blended Water - A mixture of treated and untreated water that is suitable for injection into the
distribution system. This is the same as the distributed water.

•	Percent Blend - The percent of treated water that is in the blend. Thus a 75 percent blend will refer
to water composed of 75 percent treated and 25 percent untreated water. A 100 percent blended
water is equal to treated water.

•	Maximum Distribution Flow Rate - The maximum flow rate (gallons per minute) of blended
(distributed) water which the equipment can cause to be discharged into the distribution mains on a
continuously operating basis with a nitrate level at or below 80 percent of the MCL.

•	Maximum Treatment Flow Rate - The maximum flow rate (gallons per minute) of treated water
which the equipment can produce on a continuously operating basis while maintaining the Maximum
Distribution Flow Rate.

•	Plant Factor - A factor used in computing water treatment cost. It is the fraction of total time the
plant operates or is projected to operate during its period of amortization. To standardize cost
computations, a plant factor of 50 percent will be used to determine the annual production of the
plant.

•	Percent Waste - 100 percent times the ratio of the annual wastewater production to the annual
amount of treated water production.

4.2.4 Quantitative Cost Factors

All cost data must be quantified for verification testing. Cost will be expressed as cents per 1000
gallons for operating costs and amortized capital costs.

To standardize cost computations, a plant factor of 50 percent will be used to determine the annual
production of the plant. The basis of the costs will be production of distributed water at the Maximum
Distribution Flow Rate delivered at a pressure of 60 pounds to accommodate distribution system
pressure. The total cost of all cost items will be estimated by the Manufacturer for the duration of the
testing period.

The following operation and maintenance cost items may be estimated in the PSTP:

•	Operating cost of power to operate the plant computed from the cost per kWhr.

•	Operating costs of power to boost pressure for distribution.

•	Operating cost of chemicals computed from costs as delivered by local suppliers.

•	Replacement cost of resin and/or membranes.

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•	Cost of operator labor computed from direct and indirect labor costs.

•	Cost of replacement parts. (List items)

•	Cost of maintenance. (List items)

•	Cost of service calls by equipment representatives.

•	Estimated operating cost of waste disposal. (In cents/1000 gal of blended water).
4.2.5 Quantitative Plant Performance Factors

Significant quantitative factors relating to plant performance (other than the special water quality
parameters discussed in the next section) must be included in the PSTP. These parameters relate to the
daily and annual quantities of various streams and discharges and their flow rates. These performance
factors must be estimated in the PSTP and will be verified during the testing and verification program.
Instrumentation such as flow meters, pressure gauges, conductivity meters, sampling taps, etc. must be
provided as integral parts of the equipment. A diagram should be provided to illustrate where and how
the factor can be measured and verified. If a multi-vessel ion exchange plant is tested, the
instrumentation should be located so the parameters can be checked for each vessel.

The following plant performance factors may be measured.

•	Flow rate (gpm), electrical conductivity (e.c.), and pressure of treated water during normal
treatment.

•	Flow rate (gpm), e.c., and pressure of brine stream(s) during production.

•	Flow rate (gpm), e.c., and pressure of backwash water during backwash.

•	Flow rate (gpm), e.c., and pressure of rinse water during rinse.

•	Daily amount of water treated (gallons) and delivered to distribution system.

•	Daily amount of backwash water used.

•	Daily amount of rinse water used.

•	Daily amount of wastewater produced.

•	Daily amount of make up water for brine maker.

•	Daily amount of saturated brine used for regeneration and amount used per regeneration.

•	e.c. and quantity of total wastewater produced.

•	Percent of treated water in delivered water.

In nitrate treatment, it is very helpful to evaluate overall plant performance and efficiency factors, which
will indicate the amount of chemical, added to the environment or the overall chemical costs associated
with the treatment process. For example in ion exchange, sodium chloride must be purchased,
transported and disposed of. For a reverse osmosis plant, sulfuric acid must be added for pH
adjustment and waste brines are produced. Indices of efficiency can include the following:

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•	Chemical equivalents of a specific chemical (e.g salt or sulfuric acid) required by the process to
remove one chemical equivalent of nitrate.

•	Chemical equivalents of salt materials disposed in wastewater or brine rej ect by the process for
removal of one chemical equivalent of nitrate.

•	Operating cost to remove one pound of nitrate.

•	Amount of wastewater produced by the process to remove one pound of nitrate.
4.2.6 Quantitative Factors: Plant Health, Safety and Reliability

Health, safety and reliability features are significant in equipment operation and verification testing. The
safety features treated here refer to those items which protect the water supply and hence the drinking
water public from unintended contamination. (Other types of safety features for on site plant personnel
protection are treated in Section 8). The cost effectiveness of treating water diminishes rapidly if waste
products and chemicals cause dangerous contamination because of poorly designed and/or operated
equipment. In the rush and concern to eliminate the primary contaminant at a low capital cost, the focus
on the importance of these safety features is easily lost. Primary dependence must be placed on the
reliability and quality of the equipment designer, manufacturer, and operator. It is very likely that these
features will be a primary interest of health officials who must review plant designs and issue operating
permits. The field testing personnel should be aware of these sources of contamination and may need to
devise means to check for acceptable operation.

The PSTP must describe the following:

1)	the safety features that have been designed into the equipment;

2)	what contamination problem this feature is used to address; and

3)	how the features can be field tested.

In nitrate treatment, the problem areas of concern are:

1)	Sudden loss of pressure in a distribution system being fed by a package plant can occur either
by accident or by intention during distribution maintenance procedures. Pressure loss coupled
with other equipment failures could be catastrophic unless reliable safety features are activated.
Any mechanical device presents an opportunity for failure such as valve failures due to various
mechanical causes including wear. Some valves close at slow rates or are improperly seated
upon closure. Failure of a valve to properly close can provide pathways for wastes and brines
to unintentionally enter the treated water supply. Treatment equipment pressure vessels,
manifolds and piping often serve two purposes at different times: to contain both treated water
and wastewater. Such an arrangement constitutes a direct cross connection in the event of
isolation valve failure. Connections to distributed water mains for make up water, rinse water
etc. present an opportunity for back siphoning of chemicals and waste into the distribution
system.

2)	An ion exchange bed should not be operated too long or beyond its capacity to adsorb nitrate.

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The result is not only lack of nitrate adsorption but also a phenomenon called "dumping" can
occur depending on resin types where much of the previously adsorbed nitrate is dumped back
into the treated water supply by sulfate.

3)	An ion exchange bed, after being regenerated with brine, vessels and piping should be rinsed
thoroughly of the waste salt before being put back into service. If not properly rinsed,
excessive TDS and salt could be discharged into the treated water.

4)	Introduction of waste rinse water or waste backwash water into the process stream or treated
stream is also a potential source of contamination.

In proper plant design and operation, the above areas of concern will be carefully considered, although
increased cost for safety features and extra use of rinse water and production of more wastewater may
result.

As each system will have a different piping design and operating procedure, the location and operation
of safety devices can only be generally described in this protocol document.

A simple procedure can be implemented to check for trouble spots in the event of loss of pressure in
the distribution system. Use a flow diagram showing sensors and valves as reference and ask questions
regarding any one valve, such as:

1)	If the valve is normally closed during step 1 (e.g. during treatment) in the treatment process,
could contamination result if the valve remained open? (e.g. a valve in a make up supply line
and a chemical supply tank.)

2)	If the valve is normally closed during step 2 (e.g. cleaning or rinsing), could contamination result
if the valve remained open? (e.g. a valve to the treated water line)

Examples of other operation steps can be resin or membrane cleaning, standby, brine making, rinsing,
etc. Each valve, whether normally opened or closed, can be so addressed during each different step of
the treatment process. The same queries can be made assuming sudden loss of water pressure in the
distribution line.

The following devices, piping and valve arrangements have been used to reduce unintentional
contamination in specific cases.

1)	Simple air gaps. The gaps should be frequently inspected for obstructions.

2)	Sensors for shut down or warning alarms. High nitrate and TDS alarms are effective to detect
contamination after it has occurred.

3)	Back flow preventors, check valves and double check valves allow flow in only one direction.
Addition of manually operated valves allows periodic testing by the operator.

4)	Block and bleed valve arrangements. This arrangement consists of two automatically operated
isolation valves in series with a third automatic valve between the two to act as a bleed valve.
Flow from the bleed valve is a visual indication of failure of the blocking valves.

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4.2.7 Cross Connection Control References and Guidelines

It is likely that each state will have adopted regulations covering the requirements for using cross
connection prevention devices. Although the manufacturer's equipment will be tested in a given state
(or states) it would be to the advantage to adopt measures which would apply to all states to give the
broadest market for the equipment. Useful publications are available from individual state and local
health agencies such as "Guidance Manual For Cross Connection Control Programs" published by the
State of California, Department of Health Services, Sept 1988, Public Water Supply Branch. Other
sources of information on this subject can be obtained from literature and certification training courses
given by local plumbing unions. For example, "A Course Of Instruction For Certification In Cross-
Connection Prevention" Fresno Area Plumbers, Pipe and Refrigeration Fitters JATC. The Foundation
for Cross Connection Control and Hydraulic Research at the University of Southern California, Los
Angeles, is another resource of information (213/749 2032).

The PSTP must describe the following:

1)	the safety features that have been designed into the equipment;

2)	what contamination problem this feature will address; and

3)	the recommended field testing procedure and frequency.

4.3 Water Quality Considerations

Water treatment equipment is used to treat water and change the quality of feed water (or raw water) to
reduce contaminants to a safe level. In addition, the treated water should be aesthetically pleasing and
palatable. The experimental design shall be developed so the relevant questions about water treatment
equipment capabilities can be answered.

Equipment Manufacturers should recognize that it is highly unlikely that any single process employed within a
system can successfully treat any conceivable feed water containing all of the regulated contaminants and
produce a treated water that meets the quality requirements for every regulated contaminant. Although
multiple processes could be placed in a treatment train to accomplish such a goal, for most public water
systems such comprehensive treatment capability is generally not needed and would not be cost effective.

The equipment is typically designed to treat only specific contaminants within a defined range of untreated
water quality. It is, however, possible to broaden the applicability of treatment in the case of nitrate removal
processes. In certain cases, water quality improvements regarding other constituents such as hardness,
sodium, TDS, chloride, sulfate, etc. may also be desirable. The manufacturer can use auxiliary ion exchange
treatment or membrane processes to reduce these constituents as well as the nitrate, which is the constituent
of primary concern. The Field Testing Organization should state the range of water qualities and
contamination that the equipment can successfully treat and the range of contaminants or water quality
problems that can be addressed. Manufacturers should carefully consider the capabilities and limitations of
their equipment and have the PSTP prepared to challenge their equipment sufficiently. The verification
testing should enable broad marketing for their products, while recognizing the limitations of the equipment
and not subj ecting it to testing for contaminant removal when the outcome is known in advance to be failure

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and the testing would be fruitless. The ETV Testing Plans shall be used as the basis for the specific PSTPs.

4.3.1	Feed Water Quality

One of the key aspects related to water treatment equipment performance verification is the range of
feed water quality that can be treated successfully, resulting in treated water quality that meets water
quality goals. The PSTP preparer should consider the influence of feed water quality on the quality of
treated waters produced by the equipment, such that product waters meet the water quality goals.
They should also consider the impact of various water quality parameters on the cost of the treatment
process and the quality and quantity of the waste produced. As the range of feed water quality that can
be treated by the equipment becomes broader, the potential market for treatment equipment with
verified performance capabilities will also increase. The Field Testing Organization shall specify in the
PSTP the specific water quality parameters to be monitored in the Verification Testing Program. Also,
the recommended operating range of these parameters should be stated. The following feed water
quality characteristics are important in nitrate treatment using ion exchange and membrane processes.

•	A general mineral analysis including nitrate, nitrite, chloride, sulfate, bicarbonate, carbonate, pH,
IDS, hardness, barium, silica, and all other major cations and anions.

•	Turbidity, particle concentration.

•	Temperature, with temperatures near freezing having potential for the most difficult treatment
conditions.

•	Dissolved organic carbon (DOC), total organic carbon (TOC), or UV-254 absorbance.

•	Color.

•	Density (concentration) of microorganisms (bacteria).

•	Iron and manganese.

•	Presence of algae, particularly filter clogging algae.

One of the questions often asked by regulatory engineers in approval of water treatment equipment is
"Has it been shown to work on the water where you propose to put it?" By providing treatment
capability covering a large range of water qualities the verification testing is more likely to provide an
affirmative answer to that question.

4.3.2	Treated Water Quality

Treated water quality is the most significant measurement to be made in the testing program. For nitrate
treatment processes, the Manufacturer must provide a statement of obj ectives to provide certain nitrate
levels. In addition, the Manufacturer may wish to make a statement about performance objectives of
the equipment for removal of other contaminants.

Furthermore, some water treatment equipment can be used to meet aesthetic goals. Water quality
considerations that may be important for some small systems include:

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•	color, taste and odor

•	total dissolved solids

•	iron and manganese

Finally, other water quality parameters are useful for assessing equipment performance. These may
include:

•	particle count or concentration

•	heterotrophic plate count bacteria (HPC)

•	biological dissolved organic carbon (BDOC) or assimilable organic carbon (AOC)

Other water quality considerations must also be made. Any treatment process, which removes a
contaminant also, changes the composition of other constituents. For example in ion exchange, nitrate is
removed, sulfate and alkalinity are also removed and chloride is added; in a membrane process, nitrate
is removed as well as other substances. The removal of these materials may produce a water quality
incompatible with an existing distribution system. These items must be addressed in the test plan.

4.3.3 Wastewater Characteristics, Quality and Quantity

The quality and quantity of wastewater produced by the treatment plant is a very important
consideration in testing the performance and acceptability of the plant. In many cases these factors can
be the major determining considerations in the choice of treatment. In the case of an ion exchange plant
treating for nitrate, the waste brine, although small in quantity, will be high in dissolved salts such as
sodium chloride, bicarbonate, sulfate and nitrate. The waste discharge regulator or agency, which
accepts the waste, will need to know the exact composition and quantity of waste before accepting it
for discharge. Many wastes are discharged into a local sewer line, which, in turn transfers waste to a
wastewater treatment facility. Often, the high IDS may impact the process of waste treatment or
disposal. The Manufacturer and their designated FTO should do some assessment of this impact.
Often the wastewater treatment agency will charge a fee for accepting the waste into their system. If the
nitrate treatment process is reverse osmosis, the wastewater will also contain high IDS as well as be
high in volume. The agency accepting the waste must be assured that the facilities are adequate for
accepting the waste.

In either case, the PSTP should contain the chemical composition and quantities of the various types of
waste produced by the test plant. The methods of waste discharge and disposal should be listed and
discussions and requirements of local wastewater disposal agencies should be documented in the PSTP.

4.4 Recording Water Quality Data

For all nitrate removal tests, data should be maintained on the quality parameters listed in Sections 4.3.1 and
4.3.2 above and any other data required by the plant operating and waste discharge permitting agencies as
described in Section 4.3.3. The frequency for each parameter will vary with nitrate measurements being the
most frequent. The procedures and sampling requirements shall be provided in detail in the Verification

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Testing Plan. The following items of information shall also be maintained for each experiment:

•	Type of chemical addition, dose and chemical combination, where applicable (e.g., salt, acid, chlorine,
scale inhibitor, etc.);

•	Water type (raw water, pretreated feed water, product water, wastewater);

•	Experimental run number (e.g. 1st run, 2nd run, 3rd run, etc.).

The manufacturer must provide labeled sampling taps and locations on the equipment to allow either manual
or automatic sampling. The manufacturer must also provide a diagram showing where each labeled sample
tap is located and the parameter, which can be sampled at that location.

4.5 Recording Statistical Uncertainty

For the analytical data obtained during verification testing, 95% confidence intervals shall be calculated by
the Field Testing Organization for water quality parameters in which eight or more samples were collected.
The product-specific test plan shall specify which water quality parameters shall be subjected to the
requirements of confidence interval calculation. Data quality objectives and the vendor's performance
objectives shall be used to assess which water quality parameters are critical and thus require confidence
interval statistics.

As the name implies, a confidence interval describes a population range in which any individual population
measurement may exist with a specified percent confidence. The following formula shall be employed for
confidence interval calculation:

confidence interval = X ฑt AS/Jn)

n-1,1--V	'

where: X is the sample mean;

S is the sample standard deviation;

n is the number of independent measurements included in the data set; and
t is the Student's t distribution value with n-1 degrees of freedom;
a is the significance level, defined for 95% confidence as: 1 - 0.95 = 0.05.

According to the 95% confidence interval approach, the a term is defined to have the value of 0.05, thus
simplifying the equation for the 95% confidence interval in the following manner:

95% confidence interval = Xฑtn_10 915 (s 14n)

With input of the analytical results for pertinent water quality parameters into the 95% confidence interval
equation, the output will appear as the sample mean value plus or minus the second term. The results of this
statistical calculation may also be presented as a range of values falling within the 95% confidence interval.
For example, the results of the confidence interval calculation may provide the following information: 520

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+/- 38.4 mg/L, with a 95% confidence interval range described as (481.6, 558.4).

Calculation of confidence intervals shall not be required for equipment performance results (e.g., filter run
length, cleaning efficiency, in-line turbidity or in-line particle counts, etc.) obtained during the equipment
testing verification program. However, as specified by the Field Testing Organization, calculation of
confidence intervals may be required for such analytical parameters as TOC, DOC, grab samples of
turbidity, nitrate, etc. In order to provide sufficient analytical data for statistical analysis, the Field Testing
Organization shall collect three discrete water samples at one set of operational conditions for each of the
specified water quality parameters during a designated testing period. The procedures and sampling
requirements shall be provided in detail in the Verification Testing Plan.

4.6 Instrumentation for Plant Control and Monitoring

The membrane and ion exchange equipment used for nitrate treatment are mechanical in nature and allow
the use of probes and instrumentation to monitor or control the plant operation. The PSTP should contain a
description of these controls, their function and accuracy. The following are examples.

4.6.1	Nitrate Measurements

At no time should the nitrate in the water leaving the plant (or water entering the distribution system)
exceed the MCL value. Frequent manual analysis of grab samples or automatic nitrate monitoring is
required to assess the performance of nitrate removal plants. Feed water nitrate water quality can
change suddenly; however, ion exchange processes have the capacity to handle influent nitrate
fluctuations. Monitoring this parameter in both the feed, treated and blended water is very informative
of plant performance. Measurement of nitrate on a once or twice per day basis is not sufficient to
detect rapidly changing values, which can occur. It is well known that nitrate levels can change from
minute to minute in water supplied by an intermittently operated pump. Likewise, whenever an ion
exchange vessel undergoes a regeneration cycle, potential for high nitrates exists. Some vessels are
regenerated five or more times per day. These potential fluctuations demand frequent manual or
automatic monitoring. (Note: If an automatic nitrate monitor is supplied as part of the equipment control
system, its operation should be evaluated as any other part of the plant. Such a monitor, however,
should not be used for the nitrate measurements discussed in this paragraph. If the test plan requires an
automatic monitor, one should be supplied by the Field Testing Organization).

Similarly, if membrane processes are used, nitrate variations can also occur as driving pressure changes.

4.6.2	Electrical Conductivity Measurements

Electrical conductivity measurements (e.c.) can be an indicator of plant performance and can be
measured and recorded on a continuous basis. If ion exchange is the process, this parameter will
indicate if excessive salts from improperly operating equipment has contaminated the water supply. The
excessive salts in the water supply should not exceed the secondary standards for chloride, sulfate and
IDS and should be as close to the feed water electrical conductivity as possible. If a membrane
process is used, the electrical conductivity of the product water will be less than that of the feed water

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and will be an indication of properly functioning equipment. The PSTP should indicate the range of
conductivities to be expected in the various streams.

4.7 Verification Testing Schedule

Verification testing activities include equipment set-up, startup and initial operation, verification operation,
sampling and analysis, maintenance procedures and plant shut down. Initial operations are intended to be
conducted so Manufacturers and their designated FTOs can test their equipment and be sure it is functioning
as intended. If feed water (or source water) quality influences operation and performance of equipment
being tested, the initial operations period serves as the shake-down period for determining appropriate
operating parameters.

For nitrate treatment equipment, specific care must be taken during the start up procedure to disinfect
equipment and media. Both ion exchange resins and membrane materials are sensitive to oxidants and
disinfectants. The manufacturer should provide a procedure to ensure proper disinfection of the equipment
for the start up procedure and for subsequent occasions if required.

It is recommended under this protocol that a minimum of one test period of Verification Testing of a length
specified in the appropriate test plan be conducted in order to allow testing over a period of time to collect
representative data. The specific operating and water quality parameters shall be stipulated by the selected
Test Plan under this protocol and shall be used in development of the experimental plan and the preparation
of the PSTP. Climatic changes between rainy and dry seasons or local agricultural practices may produce
substantial variability in feed water nitrate and other water quality parameters. The timing for verification
testing should consider cold weather operations because of seasonal water quality variations and because of
the impact of cold temperatures on mechanical devices, filtration and membrane devices. For instance:

•	cold temperatures (1ฐ to 5ฐC) can have an adverse effect on some water treatment processes due
to the increase in water viscosity at cold temperatures. Cold temperature considerations are
particularly important for membrane filtration applications;

•	water flows treated by many types of water treatment equipment are so great (80 to 100
liters/minute, or greater) that use of mechanical refrigeration to attain temperatures of 1ฐ to 5ฐC
would be prohibitively expensive;

•	cold temperatures have an adverse effect on mechanical pumps, chemical feed pumps, compressors
and automatically operated valves.

Verification testing with operations for which data are collected and used to verily performance are done
after initial operations are completed. The verification entity, NSF, is to be notified of the date when
verification testing is scheduled to begin.

Content of PSTP Regarding Experimental Design:

The PSTP shall contain the following:

•	Statement of the verification testing objectives.

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•	Identification and discussion of the water treatment problem or problems that the equipment
is designed to address, how the equipment will solve the problem, and who would be the
potential users of the equipment.

•	Identification of the range of key water quality parameters, given in applicable ETV Testing
Plans, which the equipment is intended to address and for which the equipment is applicable.

•	Identification of the key parameters of treated water quality that shall be usedfor evaluation
of equipment performance. Parameters of significance for treated water quality were listed
above in Section 4, and in applicable ETV Testing Plans.

•	Identification of the key qualitative parameters that shall be used for evaluation of
equipment performance. Parameters of significance for treated water quality were listed
above in Section 4, and in applicable ETV Testing Plans.

•	Identification of the key quantitative parameters that shall be used for evaluation of
equipment performance. Parameters of significance for treated water quality were listed
above in Section 4, and in applicable ETV Testing Plans.

•	Identification, description and testing procedures for safety components designed to prevent
back flow, cross connections, or any unintended contamination of treated water.

•	Detailed outline of the verification testing schedule.

5.0	FIELD OPERATIONS PROCEDURES

5.1	Equipment Operations and Design

The ETV Testing Plan specifies procedures that shall be used to ensure the accurate documentation of both
water quality and equipment performance. Careful adherence to these procedures will result in definition of
verifiable performance of equipment. (Note that this protocol may be associated with a number of different
ETV Testing Plans for different types of physical removal process equipment.)

Operation and design aspects of water treatment process equipment often provide a basis for approval or
permitting by State regulatory engineers and can be used to pinpoint specific areas of concern related to
operation of the equipment. Specific operation and design aspects to be included in the PSTP are provided
in detail, in the Manufacturer Responsibilities section below.

5.2	Communications, Documentation, Logistics, and Equipment

NSF shall communicate regularly with the verification testing participants to coordinate all field activities
associated with this verification testing and to resolve any logistical, technical, or QA issues that may arise as
the verification testing progresses. The successful implementation of the verification testing will require
detailed coordination and constant communication between all verification testing participants.

All Manufacturer, FTO and NSF field activities shall be thoroughly documented. Field documentation shall

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include field logbooks, photographs, field data sheets, and chain-of-custody forms. The qualified testing
organization shall be responsible for maintaining all field documentation. Field notes shall be kept in a bound
logbook. Each page shall be sequentially numbered and labeled with the project name and number. Field
logbooks shall be used to record all water treatment equipment operating data. Completed pages shall be
signed and dated by the individual responsible for the entries. Errors shall have one line drawn through them
and this line shall be initialed and dated.

All photographs shall be logged in the field logbook. These entries shall include the time, date, direction,
subject of the photograph, and the identity of the photographer. Any deviations from the approved final
PSTP shall be thoroughly documented in the field logbook and provided to NSF.

Original field sheets and chain-of-custody forms shall accompany all samples shipped to the analytical
laboratory. Copies of field sheets and chain-of-custody forms for all samples shall be provided to NSF.

5.3	Initial Operations

Initial operations will allow equipment Manufacturers and their designated FTOs to refine their operating
procedures and to make operation adjustments as needed to successfully treat the feed water. Information
generated through this period of operation may be used to revise the PSTP, if necessary. A failure at this
point in the verification testing could indicate a lack of capability of the process equipment and the
verification testing might be canceled.

5.4	Equipment Operation and Water Quality Sampling for Verification Testing

The qualified testing organization shall supervise equipment operation and water quality sampling and
analysis during the verification phase of testing, using the procedures described below. NSF should oversee
or inspect these activities. All field activities shall conform to requirements provided in the PSTP that was
developed and approved for the verification testing being conducted.

If unanticipated or unusual situations are encountered that may alter the plans for equipment operation,
water quality sampling, or data quality, the situation must be discussed with the NSF technical lead. Any
deviations from the approved final PSTP shall be thoroughly documented.

During routine operation of water treatment equipment, the total number of hours during which the
equipment was operated each day shall be documented as well as the time required by the operator to
perform various tasks. The qualified Testing Organization will record and verily the number of hours each
day spent by the operator of the treatment plant and provide a description of the daily tasks performed by
the operator of the treatment equipment.

Content of PSTP Regarding Field Operations Procedures:

The Field Testing Organization shall be responsible for including the following elements in the
PSTP:

• A table summary of the proposed time schedule for operating and testing,

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•	Field operating procedures for the equipment andperformance testing, based upon the ETV
Testing Plan with listing of operating parameters, ranges for feed water quality, and the
sampling and analysis strategy.

Manufacturer Responsibilities:

•	Provision of all equipment neededfor field work associated with this verification testing;

•	Provision of a complete list of all equipment to be used in the verification testing. A table
format is suggested;

•	Provision of field operating procedures.

6.0	QUALITY ASSURANCE PROJECT PLAN (QAPP)

The QAPP for this verification testing specifies procedures that shall be used to ensure data quality and
integrity. Careful adherence to these procedures will ensure that data generated from the verification testing
will provide sound analytical results that can serve as the basis for performance verification.

6.1	Purpose and Scope

The primary purpose of this section is to outline steps that shall be taken by operators of the equipment and
by the analytical laboratory to ensure that data resulting from this verification testing is of known quality and
that a sufficient number of critical measurements are taken.

6.2	Quality Assurance Responsibilities

The Field Testing Organization project manager is responsible for coordinating the preparation of the QAPP
for this verification testing and for its approval by NSF. The Field Testing Organization project manager,
with oversight from NSF, should also ensure that the QAPP is implemented during all verification testing
activities.

The Manufacturer and NSF must approve the entire PSTP including the QAPP before the verification
testing can proceed. NSF must review and either approve the QAPP or provide reasons for rejection of
the QAPP along with suggestions on how to modify the QAPP to make it acceptable, provided that the
FTO has made a good faith effort to develop an acceptable QAPP (i.e. the QAPP is 75 to 80% acceptable
with only minor changes needed to produce an acceptable plan). NSF will not write QAPPs for
Manufacturers or FTOs.

A number of individuals may be responsible for monitoring equipment operating parameters and for
sampling and analysis QA/QC throughout the verification testing. Primary responsibility for ensuring that
both equipment operation and sampling and analysis activities comply with the QA/QC requirements of the
P STP (Section 6) shall rest with the qualified testing organization, with oversight by NSF. QA/QC activities

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for the equipment shall include those activities recommended by Manufacturer and those required by NSF
to assure the verification testing will provide data of the necessary quality.

QA/QC activities for the analytical laboratory that analyzes samples sent off- site shall be the responsibility of
that analytical laboratory's supervisor. If problems arise or any data appear unusual, they shall be
thoroughly documented and corrective actions shall be implemented as specified in this section. The
QA/QC measurements made by the off-site analytical laboratory are dependent on the analytical methods
being used.

6.3 Data Quality Indicators

The data obtained during the verification testing must be of sound quality for conclusions to be drawn on the
equipment. For all measurement and monitoring activities conducted for equipment verification, NSF and
the EPA require that data quality parameters be established based on the proposed end uses of the data.
Data quality parameters include four indicators of data quality:

•	Accuracy;

•	Precision;

•	Representativeness; and

•	Statistical Uncertainty.

Treatment results generated by the equipment must be verifiable for the purposes of this program to be
fulfilled. High quality, well-documented analytical laboratory results are essential for meeting the purpose
and objectives of this verification testing. Therefore, the following indicators of data quality shall be closely
evaluated to determine the performance of the equipment when measured against data generated by the
analytical laboratory.

6.3.1 Accuracy

For water quality analyses, accuracy refers to the difference between a sample result and the reference
or true value for the sample. Loss of accuracy can be caused by such processes as:

•	errors in standards preparation;

•	equipment calibrations;

•	loss of target analyte in the extraction process;

•	interference; and

•	systematic or carryover contamination from one sample to the next.

In this Verification Testing, accuracy will be ensured by

•	maintaining consistent sample collection procedures, including sample locations;

•	timing of sample collection;

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•	sampling procedures;

•	sample preservation;

•	sample packaging;

•	sample shipping; and

•	by random spiking procedures for the specific inorganic constituents chosen for testing.

The FTO shall discuss the applicable ways of determining the accuracy of the chemical and
microbiological sampling and analytical techniques in the PSTP.

For water quality analysis, accuracy is usually expressed as the percent recovery. Percent recovery is
the amount recovered during analysis. In general percent recovery can be calculated by dividing the
measured amount added to the amount actually added.

Measured camnl(,+cnit(, - Measured	Measured

% Recovery = 	Sample+Spike	Sample * 100o/o = 	Spto * 100o/o

Actual Spike	Actual Spike

For equipment operating parameters, accuracy refers to the difference between the reported operating
condition and the actual operating condition. For equipment operating data, accuracy entails collecting
a sufficient quantity of data during operation to be able to detect a change in operations. For water
flow, accuracy may be the difference between the reported flow indicated by a flow meter and the flow
as actually measured on the basis of known volumes of water and carefully defined times (bucket and
stopwatch technique) as practiced in hydraulics laboratories or water meter calibration shops. For
mixing equipment, accuracy is the difference between an electronic readout for equipment rotations per
minute (rpms) and the actual measurement based on counted revolutions and measured time. Accuracy
of head loss measurement can be determined by using measuring tapes to check the calibration of
piezometers for gravity filters or by checking the calibration of pressure gauges for pressure filters.
Meters and gauges must be checked periodically for accuracy, and when proven to be dependable
over time, the time interval between accuracy checks can be increased. In the PSTP, the FTO shall
discuss the applicable ways of determining the accuracy of the operational conditions and procedures.

6.3.2 Precision

Precision refers to the degree of mutual agreement among individual measurements and provides an
estimate of random error. The standard deviation and the relative percent deviation recorded from
sample analyses may be reported as a means to quantify sample precision. Precision measures the
repeatability of measurement. It is usually expressed as the percent relative standard deviation (%
RSD). In general % RSD can be calculated by dividing the standard deviation by the average. The
methods to be employed for use of deviation shall be described by the FTO in the PSTP.

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2

n

	Standard Deviation

% RSD =	

Average

*100%

IS

n-1

*100%

y; = sample measurement
n = number of samples

6.3.3	Representativeness

Representativeness refers to the degree to which the data accurately and precisely represent the
conditions or characteristics of the parameter represented by the data. In this Verification Testing,
representativeness will be ensured by maintaining consistent sample collection procedures, including:

•	sample locations;

•	timing of sample collection;

•	sampling procedures;

•	sample preservation;

•	sample packaging;

•	sample shipping;

•	sample equipment decontamination; and

•	blind spikes.

Using each method at its optimum capability to provide results that represent the most accurate and
precise measurement that it is capable of achieving also will ensure representativeness. For equipment
operating data, representativeness entails collecting a sufficient quantity of data during operation to be
able to detect a change in operations.

6.3.4	Statistical Uncertainty

Statistical uncertainty of the water quality parameters analyzed shall be evaluated through calculation of
the 95% confidence level around the sample mean.

6.4 Quality Control Checks

This section describes the QC requirements that apply to both the treatment equipment and the on-site
water quality analyses. It also contains a discussion of the corrective action to be taken if the QC
parameters fall outside of the evaluation criteria.

The quality control checks provide a means of measuring the quality of data produced. The Manufacturer

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may not need to use all the ones identified in this section. The selection of the appropriate quality control
checks depends on the equipment, the experimental design and the performance goals. The selection of
quality control checks shall be based on discussions among the FTO and NSF. Some types of quality
control checks applicable to operating water treatment equipment were described in Section 6.3.4.

6.4.1	Quality Control for Equipment Operation

This section will explain the methods to be used to check on the accuracy of equipment operating
parameters and the frequency with which these quality control checks shall be made. A key aspect of
the Equipment Verification Testing Program is to provide operating results that will be widely accepted
by state regulatory engineers. If the quality of the equipment operating data cannot be verified, then the
water quality analytical results may be of no value. Because water cannot be treated if equipment is not
operating, obtaining valid equipment operating data is a prime concern for verification testing.

An example of the need for QC for equipment operations is an incident of state rejection of test data
because the treatment equipment had no flow meter to use for determining engineering and operating
parameters related to flow.

6.4.2	Water Quality Data

After treatment equipment is being operated and water is being treated, the results of the treatment are
interpreted in terms of water quality. Therefore the quality of water sample analytical results is just as
important as the quality of the equipment operating data. Most QA plans emphasize analytical QA.
The important aspects of sampling and analytical QA are given below:

6.4.2.1	Duplicate Samples. Duplicate samples must be analyzed to determine the precision of
analysis. The procedure for determining samples to be analyzed in duplicate shall be
provided with the frequency of analysis and the approximate number.

6.4.2.2	Method Blanks. Method blanks are used to evaluate analytical method-induced
contamination, which may cause false positive results.

6.4.2.3	Spiked Samples. The use of spiked samples will depend on the testing program, and the
contaminants to be removed. If spiked samples are to be used specify the procedure,
frequency, acceptance criteria, and actions if criteria are not met.

6.4.2.4	Travel Blanks. Travel blanks shall be provided to the analytical laboratory to evaluate
travel-related contamination.

6.4.2.5	Performance Evaluation Samples for On-Site Water Quality Testing. Performance
evaluation (PE) samples are samples whose composition is unknown to the analysts that
are used to evaluate analytical performance. Analysis of PE samples shall be conducted
before testing is initiated. PE samples shall be submitted by the field testing organization to
the analytical laboratory and also to the equipment testing organizations if appropriate.
The control limits for the PE samples shall be used to evaluate the equipment testing

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organization's and analytical laboratory's method performance. One kind of PE sample
that would be used for on-site QA in most studies done under this protocol would be a
turbidity PE sample.

PE samples come with statistics about each sample, which have been derived from the
analysis of the sample by a number of laboratories using EPA-approved methods. These
statistics include a true value of the PE sample, a mean of the laboratory results obtained
from the analysis of the PE sample, and an acceptance range for sample values. The
analytical laboratory is expected to provide results from the analysis of the PE samples
that meet the performance objectives of the verification testing.

6.5 Data Reduction, Validation, and Reporting

To maintain good data quality, specific procedures shall be followed during data reduction, validation, and
reporting. These procedures are detailed below.

6.5.1	Data Reduction

Data reduction refers to the process of converting the raw results from the equipment into concentration
or other data in a form to be used in the comparison. The procedures to be used will be equipment
dependent. The purpose of this step is to provide data, which shall be used to verily the statement of
performance objectives. These data shall be obtained from logbooks, instrument outputs, and
computer outputs as appropriate.

6.5.2	Data Validation

The operator shall verify the completeness of the appropriate data forms and the completeness and
correctness of data acquisition and reduction. The field team supervisor or another technical person
shall review calculations and inspect laboratory logbooks and data sheets to verily accuracy,
completeness. The individual operators and the laboratory supervisor shall examine calibration and QC
data. Laboratory and project managers shall verily that all instrument systems are in control and that
QA objectives for accuracy, completeness, and method detection limits have been met.

Analytical outlier data are defined as those QC data lying outside a specific QC objective window for
precision and accuracy for a given analytical method. Should QC data be outside of control limits, the
analytical laboratory or field team supervisor shall investigate the cause of the problem. If the problem
involves an analytical problem, the sample shall be reanalyzed. If the problem can be attributed to the
sample matrix, the result shall be flagged with a data qualifier. This data qualifier shall be included and
explained in the final analytical report.

6.5.3	Data Reporting

This section contains a list of the water quality and equipment operation data to be reported. At a
minimum, the data tabulation shall list the results for feed water and treated water quality analyses and
equipment operating data. All QC information such as calibrations, blanks and reference samples are to

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be included in an appendix. All raw analytical data shall also be reported in an appendix. All data shall
be reported in hard copy and electronically in a common spreadsheet or database format.

6.6	System Inspections

On-site system inspections for sampling activities, field operations, and laboratories shall be conducted as
specified by the ETV Testing Plan. These inspections will be performed by NSF to determine if the ETV
Testing Plan is being implemented as intended. Separate inspection reports will be completed after the
inspections and provided to the participating parties through NSF.

6.7	Reports

6.7.1	Status Reports

The equipment testing organization shall prepare periodic reports for the NSF proj ect managers. These
reports shall discuss project progress, problems and associated corrective actions, and future scheduled
activities associated with the verification testing. When problems occur, the Manufacturer and
equipment testing organization project managers shall discuss them with the NSF technical lead,
estimate the type and degree of impact, and describe the corrective actions taken to mitigate the impact
and to prevent a recurrence of the problems. The frequency, format, and content of these reports shall
be outlined in the PSTP.

6.7.2	Inspection Reports

Any QA inspections that take place in the field or at the analytical laboratory while the verification
testing is being conducted shall be formally reported by the equipment testing organizations to the NSF
project manager who will forward them to the Manufacturer and NSF QC Manager for appropriate
actions.

6.8	Corrective Action

Each PSTP must incorporate a corrective action plan. This plan must include the predetermined acceptance
limits, the corrective action to be initiated whenever such acceptance criteria are not met, and the names of
the individuals responsible for implementation. Routine corrective action may result from common
monitoring activities, such as:

•	Performance evaluation audits

•	Technical systems audits

Content of PSTP Regarding Quality Assurance Project Plan:

The Field Testing Organization shall be responsible for including the following elements in the
PSTP:

•	Description of methodology for measurement of accuracy.

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•	Description of methodology for measurement ofprecision.

•	Description of the methodologyfor use of blanks, the materials used, the frequency, the criteria
for acceptable method blanks and the actions if criteria are not met.

•	Description ofany specific procedures appropriate to the analysis of the PE samples. It has to
be clear how these samples are going to be used in the verification testing. One use of PE
samples is in the conduct of a performance audit (see Section 6.7.1).

•	Outline of the procedure for determining samples to be analyzed in duplicate, the frequency
and approximate number.

•	Description of the procedures used to assure that the data are correct.

•	Listing of equations usedfor any necessary data quality indicator calculations. These include:
precision, relative percent deviation, standard deviation, accuracy, and completeness.

•	Outline of the frequency, format, and content of reports in the PSTP.

•	Development of a corrective action plan in the PSTP.

•	Provision of all QC information such as calibrations, blanks and reference samples in an
appendix. All raw analytical data shall also be reported in an appendix.

•	Provision of all data in hard copy and electronic form in a common spreadsheet or database
format.

7.0	DATA MANAGEMENT AND ANALYSIS, AND REPORTING

7.1	Data Management and Analysis

The qualified testing organization and NSF each have distinct responsibilities for managing and analyzing
verification testing data. The field testing organization is responsible for managing all the data and
information generated during the verification testing and furnishing those records generated. The FTO will
also be responsible for analyzing the data in the verification report. NSF will be responsible for verification
of the data

A variety of data may be generated during a verification testing. Each piece of data or information identified
for collection in the ETV Testing Plan shall be provided to NSF. The data management section of the
PSTP shall describe what types of data and information needs to be collected and managed. It shall also
describe how the data shall be reported to NSF for evaluation.

Laboratory Analyses: The raw data and the validated data must be provided to NSF. These data shall be
provided in hard copy and in electronic format. As with the data generated by the innovative equipment, the
electronic copy of the laboratory data shall be provided in a spreadsheet, and a data dictionary shall be
provided. In addition to the sample results, all QA/QC summary forms must be provided.

Other items that must be provided include:

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•	field notebooks;

•	photographs, slides and videotapes (copies);

•	results from the use of other field analytical methods;

7.2 Report of Equipment Testing

The qualified testing organization shall prepare a draft report describing the verification testing that was
carried out and the results of that testing. This report shall include the following topics:

•	Introduction

•	Executive Summary

•	Description and Identification of Product Tested

•	Procedures and Methods Used in Testing

•	Results and Discussion

•	Conclusions and Recommendations

•	References

•	Appendices

•	PSTP

•	QA/AC Results

NSF will review the draft report, the results of testing, the QA/QC results, and will prepare a final report.

Content of PSTP Regarding Data Management and Analysis, and Reporting:

The Field Testing Organization shall be responsible for including the following elements in the
PSTP:

•	Description of what types of data and information needs to be collected and managed.

•	Description of how the data will be reported to NSF for evaluation.

8.0 SAFETY MEASURES

The safety procedures shall address safety considerations, which relate to the health and safety of personnel
required to work on the site of the test equipment and persons visiting the site. Many of these items will be
covered by site inspections and construction and operating permits issued by responsible agencies. They
will include:

•	Regulations covering the storage and transport of chemicals.

•	Conformance with the National Electric Code.

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•	Provision of parking facilities, sanitary facilities.

•	Provision of and access to fire extinguishers.

•	Regulations covering site security.

•	Conformance to any building permits requirement such as provision of handicap access or other health
and safety requirements.

•	Ventilation and air conditioning of equipment or of trailers or buildings housing equipment, if gases
generated by the equipment could present a safety hazard.

Content of PSTP Regarding Safety:

The PSTP shall address safety considerations that are appropriate for the equipment being tested, if
any, being used in the verification testing.

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CHAPTER 2

EPA/NSF ETV EQUIPMENT VERIFICATION TESTING PLAN
REMOVAL OF NITRATE BY REVERSE OSMOSIS
AND NANOFILTRATION

Prepared By:
NSF International
789 Dixboro Road
Ann Arbor, MI

Copyright 2002 NSF International 40CFR35.6450.

Permission is hereby granted to reproduce all or part of this work, subject to
the limitation that users may not sell all or any part of the work and may not
create any derivative work therefrom. Contact ETV Drinking Water Systems
Center Manager at (800) NSF-MARK with any questions regarding authorized
or unauthorized uses of this work.

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TABLE OF CONTENTS

Page

1.0	INTRODUCTION	2-5

1.1	Background	2-5

2.0	GENERAL APPROACH 2-5

3.0	OV ERV IEW OF TASKS	2-6

3.1	Task 1: Characterization of Feed Water	2-6

3.2	Task 2: RO/NF Performance	2-6

3.3	Task 3: Product and Waste Water Quality	2-6

3.4	Task 4: RO/NF Cleaning	2-6

3.5	Task 5: Data Reduction and Presentation	2-6

3.6	Task 6: Quality Assurance/Quality Control	2-7

4.0	TESTING PERIODS 2-7

5.0	DEFINITION OF OPERATIONAL PARAMETERS	2-8

5.1	Permeate	2-8

5.2	System F eedwater	2-8

5.3	Element Feedwater	2-8

5.4	Membrane Fouling	2-8

5.5	Stage	2-8

5.6	Feedwater System Recovery	2-8

5.7	Membrane Element Recovery	2-8

5.8	Permeate Flux	2-8

5.9	Salt Passage	2-9

5.10	Temperature Adjustment for Permeate Flow and Salt Passage Calculations	2-9

5.11	Feed-Concentrate Differential Pressure	2-9

5.12	Differential Osmotic Pressure	2-9

5.13	Net Driving Pressure	2-10

5.14	Normalized Product Flow	2-10

5.15	Normalized Salt Passage	2-10

5.16	Feed-Brine Salt Concentration	2-11

6.0	TASK 1: CHARACTERIZATION OF FEED WATER	2-11

6.1	Introduction	2-11

6.2	Objectives	2-11

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TABLE OF CONTENTS (continued)

Page

6.3	Work Plan	2-12

6.4	Analytical Schedule	2-12

6.5	Evaluation Criteria	2-14

7.0	TASK 2: RO/NF PERFORMANCE	2-14

7.1	Introduction	2-14

7.2	Objectives	2-15

7.3	Work Plan	2-16

7.4	Analytical Schedule	2-17

7.5	Evaluation Criteria	2-17

7.6	(Optional) Nitrate Spiking	2-19

8.0	TASK 3: PRODUCT AND WASTE WATER QUALITY	2-20

8.1	Introduction	2-20

8.2	Objectives	2-20

8.3	Work Plan	2-21

8.4	Analytical Schedule	2-22

8.5	Evaluation Criteria	2-22

8.5.1	Nitrate Removal	2-22

8.5.2	Fouling Indices	2-23

8.5.3	Concentrate Stream Limiting Salts	2-23

9.0	TASK 4: RO/NF CLEANING.	2-23

9.1	Introduction	2-23

9.2	Objectives	2-24

9.3	Work Plan	2-24

9.4	Analytical Schedule	2-24

9.4.1	Sampling	2-24

9.4.2	Operational Data Collection	2-25

9.5	Evaluation Criteria	2-25

10.0	TASK 5: DATA REDUCTION AND PRESENTATION	2-27

10.1	Introduction	2-27

10.2	Objectives	2-27

10.3	Work Plan	2-27

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TABLE OF CONTENTS (continued)

Page

11.0	TASK 6: QUALITY ASSURANCE/QUALITY CONTROL	2-28

11.1	Introduction	2-28

11.2	Objectives	2-28

11.3	Work Plan	2-28

11.3.1	Daily QA/QC Verifications	2-28

11.3.2	Weekly QA/QC Verifications	2-29

11.3.3	Quarterly QA/QC Verifications	2-29

11.3.4	On-Site Analytical Methods	2-29

11.3.4.1	pH	2-29

11.2.4.2	Turbidity	2-29

11.3.5	Chemical and Biological Samples Shipped Off- Site for Analysis	2-29

12.0	OPERATION AM) MAINTENANCE	2-31

12.1	Operation	2-31

12.2	Maintenance	2-32

12.2.1 Troubleshooting	2-32

13.0 REFERENCES	2-33

TABLES

Table 1. Raw Water Characterization	2-13

Table 2. Membrane Treatment System Information to be Provided in PSTP	2-18

Table 3. Sample Operational Data Collection Matrix	2-19

Table 4. Sample Water Quality Data Collection Matrix	2-22

Table 5. Data to be Recorded for Documentation of Cleaning Efficiency	2-26

Table 6. Analytical Methods	2-30

FIGURES

Figure 1. Membrane Treatment System Verification Testing Schedule	2-7

Figure 2. Sample Monitoring Points for 2-Stage Treatment System with Concentrate

Recycle and Raw Water Bypass	2-19

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1.0

INTRODUCTION

1.1 Background

This document is the ETV Testing Plan for Reverse Osmosis and Nanofiltration Processes for the Removal
of Nitrates from Contaminated Water. This Testing Plan is to be used as a guide in the development of
Product-Specific Test Plan (PSTP) procedures for testing reverse osmosis and nanofiltration (RO/NF)
treatment equipment, within the structure provided by the ETV Protocol Document for nitrate removal.
Refer to the Test Plans for Equipment Verification Testing for Physical-Chemical Removal of Nitrate by Ion
Exchange and Membrane Processes for further information.

This document is applicable only to pressure-driven membrane processes such as RO/NF. This document
is NOT applicable to electrically-driven, thermally-driven, or concentration-driven membrane processes.

Standard pretreatment such as cartridge filtration and acid and/or antiscalant addition included in a RO/NF
treatment system that is to be evaluated for removal of nitrates is considered an integral part of the treatment
system. In such cases, the system shall be considered as a single unit and the pretreatment process shall not
be separated for optional evaluation purposes.

Additional pretreatment processes which may be required to reduce particle loading to the RO/NF system
for surface water applications are considered to be a separate treatment module whose performance and
operation are outside the scope of this document. Where such pretreatment is required to meet reduce the
fouling potential of the RO/NF feedwater as measured by silt density index and turbidity values.

In order to participate in the equipment verification process for RO/NF processes, the Equipment
Manufacturer shall retain an NSF-qualified Field Testing Organization (FTO) to employ the procedures and
methods described in this test plan and in the referenced ETV Protocol Document as guidelines for the
development of the PSTP. The procedures shall generally follow those Tasks related to Verification Testing
that are outlined herein, with changes and modifications made for adaptations to specific equipment. At a
minimum, the format of the procedures written for each Task should consist of the following sections:

•	Introduction

•	Objectives

•	Work Plan

•	Analytical Schedule

•	Evaluation Criteria

2.0 GENERAL APPROACH

Testing of equipment covered by this Verification Testing Plan will be conducted by an NSF-qualified
Testing Organization that is selected by the Manufacturer. Water quality analytical work to be carried out
as a part of this Verification Testing Plan will be contracted with a state-certified or third party- or EPA-

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accredited laboratory.

The FTO shall provide full detail of the procedures to be followed for each task in the PSTP. The FTO
shall specify the operational conditions to be evaluated during the Verification Testing.

3.0	OVERVIEW OF TASKS

This ETV Testing Plan is divided into 6 tasks. A brief overview of the tasks to be included in the
verification testing program is presented below.

3.1	Task 1: Characterization of Feed Water

A full characterization of the source water must be made prior to initiating operation so that the potential for
fouling and mineral precipitation (scaling) can be defined. Results of this analysis will be used to define
feedwater pretreatment requirements and system operating conditions, and to identify potential foulants in
the source water for monitoring during operation.

3.2	Task 2: RO/NF Performance

The objective of this task is to evaluate RO/NF operation. RO/NF productivity and the rate of fouling will
be evaluated in relation to feed water quality. The relative fouling rates will be used, in part, to evaluate
operation of the RO/NF equipment under the flux and recovery conditions to be verified.

3.3	Task 3: Product and Waste Water Quality

The objective of this task is to evaluate the quality of water produced by the RO/NF system, referred to as
product water or permeate. Multiple water quality parameters will be monitored during each operational
period. A basic goal of this Task is to confirm that RO/NF-treated waters meet the manufacturer's
statement of performance objectives for nitrate. Permeate quality will be evaluated in relation to feed water
quality and operational conditions. The waste water (concentrate) stream will also be characterized.

3.4	Task 4: RO/NF Cleaning

An important aspect of RO/NF operation is the restoration of membrane productivity after fouling has
occurred. The objective of this task is to evaluate the efficiency of membrane cleaning. Normalized
product flow, normalized salt passage, and differential pressure before and after cleaning will be used as the
primary criteria for evaluation of cleaning effectiveness.

3.5	Task 5: Data Reduction and Presentation

The objective of this task is to establish an effective field protocol for data management at the field
operations site and for data transmission between the FTO and NSF for data obtained during the

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Verification Testing.

3.6 Task 6: Quality Assurance/Quality Control

An important aspect of Verification Testing is the protocol developed for quality assurance and quality
control. The objective of this task is to assure accurate measurement of operational and water quality
parameters during Verification Testing.

4.0 TESTING PERIODS

If the source water is a groundwater which exhibits little or no significant changes in seasonal water quality,
the required operational tasks in the Verification Testing Plan (Tasks 1-4) shall be performed once
(minimum) or twice (preferred) over a one-year period. Each test run, excluding equipment mobilization,
startup/troubleshooting, and demobilization is to be based on a minimum of 1,000 hours of membrane
system operation.

A schedule describing the sequence and duration of each of the required tasks is provided in Figure 1. In
the rare event that the source water is a surface water, the operational tasks shall be performed for four
weeks each quarter over a calendar year.

Task Name

We Wee We We Wei Wei Wei We Wc Wee Week 11

Task 1: Characterization of Feed Water

















Review Historical Data

i

i

1st Grab Sample

~



2nd Grab Sample



~













Results Compiled





~











Task 2: Membrane Flux and Recovery











E quipm ent S etup

ฆ

i

Startup and Troubleshooting



ฆ

Membrane Setting

c

=i

Generate Flux Decline Curve



i



i

Task 3: Product and Waste Water Quality

Generate Samples

i



i





Analysis





:



n

Task 4: Membrane Cleaning





Clean

















Operate to Confirm Cleaning Effectiveness

I

II

Task 5: Data Reduction and Presentation













Compile Operational Data

ฆ







i



r	1

Data Reduction









I

I

Data Completed



~

Task 6: Quality Assurance/Quality Control

i i

Figure 1 - Membrane Treatment System Verification Testing Schedule

(Single Test Period)

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5.0 DEFINITION OF OPERATIONAL PARAMETERS

The following definitions are used to characterize performance of the RO/NF system.

5.1	Permeate: Product water produced by the RO/NF treatment system.

5.2	System Feedwater: Source water introduced into the RO/NF treatment system for treatment.

5.3	Element Feedwater: Water introduced into the RO/NF element, consisting of system feedwater
for single-pass systems, or a combination of system feedwater and recycled concentrate for systems with
concentrate recycle.

5.4	Membrane Fouling: A reduction in permeate flux caused by the accumulation of feedwater
contaminants within or on the surface of the membrane or within or on the feedwater spacer. Fouling that
can be restored by hydraulic or chemical means is termed "reversible" fouling. In contrast, "irreversible"
fouling is defined as a permanent loss in permeate flux that cannot be restored by hydraulic or chemical
means.

5.5 Stage: An assemblage of one or more pressure vessels, each containing between three and seven
membrane elements, plumbed to receive a common feedwater. Each vessel receives approximately equal
feed flow, produces approximately equal permeate and concentrate flow and operates at equal recovery.

5.6 Feedwater System Recovery: The ratio of permeate flow to system feedwater flow, expressed
as a percentage:

% System Recovery = 100 x

Qf

Where:

QP
Qf

= Permeate flow rate
= Feed flow rate to the membrane system

5.7 Membrane Element Recovery: The ratio of permeate flow to element feedwater flow, expressed
as a percentage:

0,

% Element Recovery = 100 x

Qf + Qr

Where:

Qp

= Permeate flow rate

Qf = System feed flow rate to the element

Qr = Concentrate recycle flow rate to the element (if present)

5.8 Permeate Flux: The flow of permeate produced by the RO/NF system divided by the total
membrane surface area of all elements in the system. Permeate flux is calculated according to the following

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formula:

• s

Where:	Jt = Permeate flux at time t (gallons per square foot per day)

Qp = System permeate flow at time t (gpd)
S = Membrane surface area (ft2)

5.9 Salt Passage: The ratio of the concentration of any salt present in the permeate to its
concentration in the feed stream, expressed as a percentage:

Cp

SP = —r~ x 100

cf

Where:	SP = Salt passage

Cp = Permeate concentration for a given salt (mg/L)
Cf = Feed concentration for a given salt (mg/L)

5.10	Temperature Adjustment for Permeate Flow and Salt Passage Calculations: Flow of water
and salt through a RO/NF membrane is proportional to feedwater temperature based primarily on the
viscosity of water. Permeate flow and salt passage must be corrected to a reference temperature of 25ฐC
to enable an accurate determination of how changes in these parameters are affected by feedwater
constituents according to the following equation:

JT

25 ~ 1.03(t-25)

Where:	J25 = Instantaneous flux at reference temperature of 25ฐC (gfd)

JT = Instantaneous flux at operating temperature T (gfd)
T = Operating temperature (ฐC)

In many cases, membrane manufacturers have developed temperature correction factor (TCF) values
specific to their membrane products that are more accurate than the equation shown above. Where
available, the manufacturer-provided TCF should be used.

5.11	Feed-Concentrate Differential Pressure: The difference in measured pressure between the
feed stream and the concentrate stream of a stage of the membrane array or of the entire membrane system.
Expressed as an equation:

AP = Pf~Pc

Where:	AP = Feed-concentrate differential pressure (psi)

Pf = Feed stream pressure for a stage or the system (psig)
Pc = Concentrate stream pressure for a stage or the system (psig)

5.12 Differential Osmotic Pressure: The difference in osmotic pressure between the feed and

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permeate streams. Osmotic pressure of the feed is defined as average osmotic pressure of the feedwater
into and the concentrate out of the membrane system. Osmotic pressure is a measure of the force exerted
by the natural tendency of water to flow across a semi-permeable membrane from a solution of lower salt
concentration to a solution of higher salt concentration. Expressed as a formula:

Att =

( TDS f + TDS c
2

-msr

xO.Ol

Where:	A 71	=	Differential osmotic pressure (psi)

TDSf	=	Feedwater total dissolved solids (mg/L)

TDSC	=	Concentrate total dissolved solids (mg/L)

TDSp	=	Permeate total dissolved solids (mg/L)

5.13 Net Driving Pressure: The pressure available to drive water through the membrane, equal tothe
average feed pressure (average of feed pressure and concentrate pressure) minus the differential osmotic
pressure, minus the permeate pressure. Expressed as a formula:

NDP =

(p,+p.\

- A7T- P„

Where:	NDP	=	Net Driving Pressure (psi)

Pf	=	Feed pressure (psi)

Pc	=	Concentrate pressure (psi)

A71	=	Differential osmotic pressure (psi)

Pp	=	Permeate pressure (psi)

5.14 Normalized Product Flow: To clearly observe changes in permeate flux caused by membrane
fouling or scaling, measured permeate flow must be corrected or "normalized" for variations in Net Driving
Pressure and Temperature, using the following formula:

NDP TCF

NPF = 	~x	'-xQ

NDPt TCFt

Where:

NPF
NDP;
NDPt
TCF;

TCFt
Qp

Normalized product flow (gpm)

Net Driving Pressure at initial conditions of operation (psi)
Net Driving Pressure calculated at time t (psi)

Temperature Correction Factor based on temperature at initial
conditions of operation

Temperature Correction Factor based on temperature at time t
permeate flow (gpm)

5.15 Normalized Salt Passage: To more clearly observe changes in the flow of any salt through the
membrane caused by membrane fouling and scaling or changes in the permeability of the membrane itself

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from exposure to feedwater constituents, salt passage is normalized using the following equation:

NDPt Cfi t Cft

NSP=mxtxtxSP

Where:	NSP	=	Normalized salt passage (%)

NDP;	=	Net Driving Pressure at initial conditions of operation (psi)

NDPt	=	Net Driving Pressure calculated at time t (psi)

Cat	=	Feed-brine salt concentration at timet (see below)

Cft ;	=	Feed-brine salt concentration at initial conditions of operation (see
below)

Cft	=	Feed salt concentration at time t (mg/L)

Cfi	=	Feed salt concentration at initial conditions of operation (mg/L)

SP	=	Salt passage (ฐA

5.16 Feed-Brine Salt Concentration: The Feed-Brine salt concentration used in the calculation of
Normalized salt Passage is defined by the following equation:

In

rC^

b

cfl=-

yCfJ

1-

ฃl

.a

Where:	Cft = Feed-Brine salt concentration

Cb = Brine (concentrate) salt concentration (mg/L)
Cf = Feed salt concentration (mg/L)

6.0	TASK 1: CHARACTERIZATION OF FEED WATER

6.1	Introduction

This task involves a complete characterization of the raw water being fed to the treatment system. The
information is required to determine the suitability of the water source as feed water for verification testing,
and to document parameters which may be important in predicting the fouling and scaling tendencies of the
water source.

6.2	Objectives

The objectives of this task are as follows:

• Obtain a complete chemical and physical characterization of the source water or feed water that
will be subject to treatment.

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•	Identify potential membrane foulants and sealants (turbidity, bacteria, sparingly soluble salts,
etc.) that will determine the type and degree of feedwater pretreatment and that must be
monitored during system operation.

•	Verify that the water as sampled is representative of the source water based on historical data
(where available).

6.3	Work Plan

This Verification Testing Plan is based on the assumption that RO/NF for nitrate removal will be
predominately applied to groundwaters that are not subject to significant seasonal changes in water quality.
Application of membrane treatment systems to surface waters requires a significantly different approach than
that outlined here in order to address seasonal variations in water quality.

Most water sources will not have pre-existing water quality data of sufficient detail to allow an evaluation of
the proper application of RO/NF. Completion of this task involves the following:

•	Analysis of grab samples for a detailed water quality analysis. The parameters evaluated will
allow calculation of a complete cation/anion balance, in addition to general physical/chemical
measurements and limited microbiological and organic analysis.

•	A review of selected historical water quality data, where available. This will allow
determination of trends in key water quality parameters such as nitrate and IDS or
conductivity, as well as allowing verification that the water quality measured by the grab
samples is representative of the recent historical data.

•	Calculation of the scaling potential of the source water to be treated. This includes estimating
the concentrations of the following salts in the membrane concentrate stream at the membrane
system operating conditions proposed by the Manufacturer in Task 2 to the degree, if any, that
these salts will be present in the concentrate stream in excess of their theoretical solubility:

-	calcium carbonate

-	calcium sulfate

-	barium sulfate

-	strontium sulfate

-	calcium fluoride

-	Silica

The FTO shall include in the PSTP guidelines for maximum percent saturation for each of the above salts
during RO/NF system operation assuming the use of appropriate scale inhibiting chemicals.

6.4	Analytical Schedule

Parameters required for a complete evaluation of source water quality are presented in Table 1. Table 1

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identifies required and optional parameters for evaluation by analysis of grab samples.

Table 1



Raw Water Characterization

Parameter

Grab Samples

General/Physical Parameters



Temperature

Required



pH

Required



TDS

Required



Conductivity

Required



Silt Density Index

Required



Turbidity

Required



Particle Counts

Optional



Color

Optional



Taste and Odor

Optional

Inorganic Cation/Anion Balance



Ca+2

Required



Mg+2

Required



Na+

Required



r

Required



NH,+

Optional



Sr+2

Required



Ba+2

Required



Fe+2

Required



Mn+2

Required



O
O

Required



hco3-

Required



S04-2

Required



cr

Required



no3-

Required



F"

Required



o
o

Required



h2s

Optional



Si02

Required

Organic/Microbiological



Total Organic Carbon

Required



Total Coliforms

Optional



Heterotrophic Plate Count

Required



UV absorbance (@254 nm)

Optional



AOC/BDOC

Optional

(1) Report the mean and standard deviation of a mini-mum of

two grab samples taken at least 10 days apart.

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Parameters to be analyzed from grab samples should be taken from a minimum of 2 samples taken at least
10 days apart. Potential sources of historical data include the United States Geological Survey, US
Environmental Protection Agency, and state and local laboratories.

Manufacturers intending to have their equipment verified for uses other than nitrate removal may wish to
characterize the source water in terms of additional parameters besides those identified in Table 1.

6.5 Evaluation Criteria

Feed water quality will be evaluated in the context of the Manufacturer's statement of performance
objectives. The feed water should challenge the capabilities of the equipment with respect to nitrate
concentration but should not be beyond the range of water quality suitable for treatment for the equipment in
question.

The detailed water quality analysis results will allow an estimation of which sparingly soluble salts, if any,
present a potential for scaling by mineral precipitation at the water temperature and recovery conditions to
be tested. The analysis will allow proper selection of the chemical pretreatment (acid addition and/or
antiscalant addition) and the design recovery of the RO/NF system. The water quality analysis will also
determine if feedwater pretreatment is required to reduce fouling tendency. If turbidity or silt density index
values exceed membrane-industry accepted criteria or if microbiological indicators suggest that biological
fouling potential is significant, the Manufacturer will be required to provide pretreatment to adequately
address these concerns.

7.0	TASK 2: RO/NF PERFORMANCE

7.1	Introduction

The purpose of this task is to verily that the RO/NF system, when tested in accordance with Manufacturer-
selected operating conditions on the selected source water, can maintain performance as defined by:

•	Productivity (product flow)

•	Permeate nitrate concentration (and other salts, if applicable)

•	Feedwater system recovery over a specified period of operation

A further purpose of this task is to demonstrate that changes in the level of these performance characteristics
caused by membrane fouling or other interactions between the RO/NF system and the feedwater can be
adequately managed through chemical cleaning of the membrane elements at an acceptable frequency.

In this task, the RO/NF system will be operated at conditions of constant permeate flux and recovery as
specified by the FTO, and the normalized product flow, normalized salt passage (as measured by

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conductivity) and their changes with operating time will be measured. As fouling occurs and normalized
product flow declines or normalized salt passage increases to pre-determined values (proposed by the FTO
and agreed to by NSF), the RO/NF system will be chemically cleaned per Task 4 to remove foulants and if
possible, sealants. The efficiency of cleaning will then be assessed by measuring the degree to which
normalized product flow has been increased and/or normalized salt passage has been decreased upon
subsequent operation of the RO/NF system.

In the event that fouling rates are judged to be excessive and/or chemical cleaning efficiency less than
desired, the Manufacturer shall propose revised operating conditions to reduce fouling rate. The effect of
the new conditions of membrane productivity will then be determined by additional testing.

Prior to the start of the Verification Testing Program, the operational conditions to be verified shall be
specified by the FTO in terms of an average permeate flux (gfd), feedwater recovery, and maximum salt
passage (or its converse, minimum salt rejection) at a reference temperature of 25ฐC.

The degree of fouling or scaling that occurs within a RO/NF system is a function of source water quality and
operational conditions. Waters with high particle loads or greater concentrations of sparingly soluble salts
generally produce increased fouling and scaling. Feedwater, permeate and concentrate streams will be
sampled for water quality parameters critical to the assessment of membrane productivity as they relate
directly to fouling or scaling potential. This sampling will be conducted in conjunction with sampling
performed under Tasks 1 and 3. Flow, temperature, pressure and conductivity data shall be collected to
quantify changes in the following parameters:

•	Normalized product flow

•	Normalized salt passage

•	Feed-concentrate differential pressure

The testing runs conducted under this task shall be performed in conjunction with Tasks 3 and 4. With the
exception of additional testing periods conducted at the FTO's discretion, no additional RO/NF test runs
are required for performance of Tasks 3 and 4. This task shall be performed once (minimum) or twice
(preferred, within a one-year period, with a minimum of 6 months between test runs).

7.2 Objectives

The objectives of this task are to document the following:

•	Operational conditions for the RO/NF system.

•	Feedwater system recovery achieved by the RO/NF equipment.

•	The rate of change in normalized product flow, salt passage and feed-concentrate differential
pressure and associated operating times between cleanings based on these rates.

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Verification of RO/NF system operation shall also apply to operating conditions that are considered less
stringent than those conditions tested; examples of less stringent conditions would include operation at lower
membrane flux (lower permeate flow) and lower product water recovery.

7.3 Work Plan

The PSTP shall specify information concerning design and operation of the RO/NF treatment system being
evaluated, using the following categories as specified in Table 2:

•	System design criteria

•	Operating conditions (including those for pretreatment and RO/NF systems)

•	Written procedures for operation and maintenance

•	Cleaning Criteria. Specify allowable changes to the following parameters, which indicate a
need for cleaning of a stage of the array or the entire system:

Percent loss of normalized product flow

\rpp _ \rpp

, T1-.	T	Original	Fouled

NPF % Loss = —

NPF

Original

Percent increase in normalized salt passage

AA75 - AW

-xron T	Fouled	Original

NSP % increase = —

N^P

Original

Percent increase in feed-concentrate differential pressure (across each stage and/or
the RO/NF system)

APf , , — AP . . ,

a t-\ r\ / t	rouled	Original

AP % Increase = -

Original

After startup of the RO/NF equipment, membrane operation should be established at the permeate flux and
recovery conditions to be verified. In the event the temperature of the feedwater differs significantly from
25ฐC, the Manufacturer shall provide a temperature-specific permeate flux (normalized to account for
differences in temperature between Manufacturer-specified and actual). The RO/NF system may be
operated for up to 24 hours to allow the membrane elements to come to equilibrium prior to the start of
data used in the flux decline calculations (membrane setting).

Following the membrane setting period, the treatment system should be operated until one or more of the
cleaning criteria specified in the PSTP are met or a total of 1,000 hours of run time is achieved (whichever
occurs first). The obj ective of operation is to attain 1,000 hours or operation without the need for chemical
cleaning. If the rate of change in normalized product flow, normalized salt passage or feed-concentrate
differential pressure results triggers one or more cleaning criteria before the 1,000-hour operating period is
complete, chemical cleaning shall be performed per Task 4 and adjustments to operation shall be made to

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reduce the rate of change in these performance parameters (such as a decrease in permeate flux or
feedwater system recovery).

Decisions on operating condition adjustments shall be made based upon the Manufacturer's experience and
consultation with the FTO responsible for performing the study. If subsequent operation at the new
conditions results in the need for a second cleaning prior to the attainment of 1,000 operating hours,
chemical cleaning shall again be conducted and cleaning efficiency determined. RO/NF system operating
conditions shall then be further adjusted to provide for an acceptable rate of change to attain 1,000 hoursof
operation between cleanings. Each recommended change in operating conditions shall be first approved by
NSF and the FTO.

During operation, data for the operational parameters identified in Table 3 should be monitored and
recorded either continuously by means of on-line instrumentation, or at a minimum of twice daily by manual
measurement. Requirements for water quality monitoring during operation are presented in Task 3.

Additional testing may also be included in the PSTP in order to demonstrate RO/NF performance under
different feedwater quality conditions. The FTO shall perform testing with as many different water quality
conditions as desired for verification status. Testing under each different water quality condition shall be
performed during an additional 1,000-hour testing period, as required above for each additional set of
operating conditions.

7.4	Analytical Schedule

A sample matrix of operation monitoring points, parameters, and frequency for a typical two- stage RO/NF
treatment system with concentrate recycle (see Figure 2) is presented in Table 3. The manufacturer should
adopt the operational data collection locations to the particular geometry of the RO/NF system. In general,
adequate data must be documented to allow evaluation of each stage of the system independently, as well
as documenting operation of the treatment system as a whole.

7.5	Evaluation Criteria

Provide tabular data for the parameters listed in Table 3.

Provide graphs of the following parameters versus elapsed run time:

•	Temperature

•	Flux

•	Recovery

•	Feed Pressure

•	Normalized product water flow

•	Normalized salt passage

•	Feed-concentrate differential pressure (across each stage)

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Table 2



Membrane Treatment System Information to be Provided in Manufacturer PSTP

Parameter, units

Comments

System Configuration





Number of stages





Number of pressure vessels in each stage





Number of membrane elements per pressure vessel





Surface area per membrane element, Ft2





Acid addition

Type and dose



Antiscalant addition

Type and dose



Cartridge filtration, |j,m

Nominal rated pore size



Other pretreatment

Describe if used

Operating Conditions to be Evaluated





Recovery per stage, %





Recovery for system, %





Design flux, gfd





Feed water temperature





Feed water pH





Feed water nitrate concentration





Feed water TDS





Concentrate recycle rate, gpm or %



Operations and Maintenance Procedures





System startup





Normal operation





Temporary system shutdown (flush)

System shutdown < 48 hours



Prolonged system shutdown (preservation)

System shutdown >48 hours

Cleaning Criteria





Allowable normalized product flow decline, %

Percent reduction from initial value



Allowable increase in differential pressure, %

Percent increase from initial value



Allowable normalized salt passage increase, %

Percent increase from initial value

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NITRATE

Figure 2 - Sample Monitoring Points for 2-Stage Treatment System
with Concentrate Recycle and Raw Water Bypass

Table 3

Sample Operational Data Collection Matrix

Monitoring Location

(Refer to Figure 2)(1)

Temper-
ature

Flow

Pressure

Conduct-
ivity

1

Raw Water









2

Membrane Feed Water









3

Stage 1 Permeate









4

Stage 1 Concentrate









5

Stage 2 Permeate









6

System Concentrate









7

System Permeate









7

Blended Product Water









(1) Adopt the operational data collection locations to the particular geometry of
the membrane system

(2)

indicates no monitoring requirement

7.6 (Optional) Nitrate Spiking

If the nitrate concentration at the test site does not challenge the treatment system to the Imits of its
performance objectives, an optional nitrate augmentation procedure may be used after the required 1,000
hour operating period is completed. Nitrate spiking would allow demonstration of product water quality
under conditions of elevated nitrate in the feed water.

To spike nitrate, use of an appropriate spiking solution and metering pump will be required. A solution

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prepared from a monovalent nitrate salt (sodium nitrate, potassium nitrate) is preferred to avoid inadvertent
addition of a cation that might increase the scaling potential of the test water. Use of nitric acid as nitrate
source is not recommended because it would interfere with proper documentation of the acid dose required
to prevent scaling.

Where nitrate spiking is proposed, the FTO must detail procedures for preparation of the spiking solution,
and procedures for proper mixing of the spiking solution into the feedwater. The spiking solution must be
added to the feed water prior to any other chemical addition (acid or antiscalant), prior to the point of
concentrate recycle into the feed water (if used), and prior to raw water bypass for permeate blending.
Refer to Figure 2 for an example of a proper spiking solution addition point.

Where nitrate spiking is proposed, the FTO may choose to operate over a range of feed nitrate
concentrations. For each target nitrate concentration to be tested, the system should be operated for at
least 5 days (120 hours) to allow steady-state performance to be achieved.

8.0	TASK 3: PRODUCT AND WASTE WATER QUALITY

8.1	Introduction

This task involves a characterization of product and waste quality during the system operation described in
Task 2. Product water analysis will serve to document that the treatment system meets the nitrate removal
performance criteria for which the manufacturer is seeking verification. Additional water quality information
is required to identify performance of the treatment system relative to any potential foulants identified during
the raw water characterization performed in Task 1.

The quality and quantity of concentrate produced by the RO/NF treatment system is a very important
consideration in determining the efficacy and cost-effective use of the RO/NF treatment system. Regulators
responsible for permitting the safe and environmentally acceptable disposal of the concentrate typically
require precise information regarding physical, chemical and microbiological characteristics, along with other
information relating to the biotoxicity of the concentrate. Costs for concentrate disposal can be significant
based on the type of disposal option selected, particularly for those not utilizing a direct discharge to a
surface water body.

8.2	Objectives

The objectives of this task are as follows:

• Assess the ability of the RO/NF equipment to meet the water quality goals specified by the
Manufacturer.

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•	Monitor the concentrations of any potential foulants and sparingly soluble salts that may
interfere with the long-term operation of the treatment system. Examples include turbidity,
calcium, alkalinity, and bacterial plate counts in the feed water as identified in Task 1.

•	Characterize the volume and composition of the wastewater (concentrate) produced by the
process.

8.3 Work Plan

Water quality data shall be collected for the RO/NF treatment system feedwater, permeate and concentrate
as shown in Table 4, during the RO/NF test runs of Task 2. At a minimum, the required sampling schedule
shown in Table 4 shall be observed by the FTO on behalf of the Manufacturer. Water quality goals and
target removal goals for the RO/NF equipment shall be clearly delineated in the PSTP.

A list of the minimum number of water quality parameters to be monitored during equipment verification
testing is provided in the Analytical Schedule section below and in Table 4. The actual water quality
parameters selected for testing and monitoring shall be stipulated in the PSTP. The limiting salt cation and
anion listed in Table 4 shall be determined from source water analyses and estimation of concentrate stream
concentrations of the sparingly soluble salts as required under Task 1. Each salt that has been determined
to be present in the concentrate at levels exceeding theoretical solubility or for which chemical conditioning
of the feedwater is required to control solubility shall be monitored per the requirements of Table 4.

The FTO shall identify the treated water quality objectives to be achieved in the statement of performance
objectives of the equipment to be evaluated in the Verification Testing Program. The statement of
performance objectives prepared by the FTO shall indicate the range of water quality under which the
equipment can be challenged while successfully treating the feedwater.

Although this Verification Testing Plan and the associated protocol are oriented towards removal of nitrate,
the Manufacturer may desire to evaluate the treatment system's removal capabilities for additional water
quality parameters.

Many of the water quality parameters described in this task shall be measured on- site by the FTO (refer to
Table 5). Analysis of the remaining water quality parameters shall be performed by a state-certified or third
party- or EPA-accredited analytical laboratory.

The analytical methods utilized in this study for on- site monitoring of feedwater and permeate water qualities
are described in Task 6, Quality Assurance/ Quality Control (QA/QC).

Where appropriate, the Standard Methods reference numbers and EPA method numbers for water quality
parameters are provided for both field and laboratory analytical procedures.

For the water quality parameters requiring analysis at a state-certified or third party- or EPA-accredited
laboratory, water samples shall be collected in appropriate containers (containing necessary preservatives as

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applicable) prepared by the laboratory. These samples shall be preserved, stored, shipped and analyzed in
accordance with appropriate procedures and holding times, as specified by the analytical lab.

Table 4

Sample Water Quality Data Collection Matrix

Monitoring Location

(Refer to Figure 2)

(1)

Twice per Day

Once per Day

Once Every 5 Days

PH

Nitrate

Turbidity

Silt
Density
Index

Other
Potential
Foulants

(2)

Alka-
linity

Calcium
Hardnes
s

Limiting
Salt
Cation

(3) (4)

Limiting
Salt
Anion

(3) (4)

Other
Sealants

(3) (4)

1

Raw Water





















2

Membrane Feed
Water





















3

Stage 1 Permeate





















4

Stage 1 Concentrate





















5

Stage 2 Permeate





















6

System Concentrate





















7

System Permeate





















8

Blended Product
Water





















(1)	Adopt the operational data collection locations to the particular geometry of the membrane
system

(2)	If identified from raw water quality analysis, Task 1

(3)	As determined from raw water quality analysis, Task 1

(4)	Limiting salts shall include one or more of the following: CaS04, BaS04, SrS04, Si02, and CaF2

8.4	Analytical Schedule

The minimum monitoring frequency for the required water quality parameters is presented in Table 4. Atthe
discretion of the FTO, the water quality sampling program may be expanded to include a greater number of
water quality parameters and to require a greater frequency of parameter sampling.

Sample collection frequency and protocol shall be defined explicitly by the FTO in the PSTP; however, to
the extent possible, analyses for inorganic water quality parameters shall be performed on water sample
aliquots that were obtained simultaneously from the same sampling location, in order to ensure the maxiirum
degree of comparability between water quality analytes.

8.5	Evaluation Criteria
8.5.1 Nitrate Removal

The primary evaluation criteria will be the ability to meet the degree of nitrate rejection (expressed as a
percentage) claimed by the manufacturer for the application being verified.

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Provide a graph showing the RO/NF feedwater and permeate nitrate concentrations as a function of
elapsed operating time.

Provide a graph of nitrate rejection as a function of elapsed operation time, as defined by the following:

Nitrate Rejection (%) =

'Cf-C.^

J	i

Cf J

x 100

Where:	Cf = Nitrate concentration in the feed water (mg/L)

Cp = Nitrate concentration in the product water (mg/L)

8.5.2 Fouling Indices

Provide graphs of RO/NF system feedwater turbidity and silt density index (SDI) as a function of
elapsed operating time.

Provide a table showing maximum, minimum, and average RO/NF system feedwater turbidity and SDI
values over the entire period of operation. The table shall include a listing of the RO/NF
manufacturer's recommended maximum turbidity and SDI values to ensure satisfactory long-term
operation of the RO/NF elements and to ensure that the element warranty is not voided.

8.5.3 Concentrate Stream Limiting Salts

Provide graphs of each limiting salt that was present in the system as a function of elapsed operating
time. These graphs are required only where concentration of the salt is greater than theoretical
solubility or where chemical conditioning of the feedwater was used to control solubility.

Provide a table showing maximum, minimum, and average concentrate stream scaling indices over the
entire period of operation, or where the RO/NF system was operated at more than one feedwater
recovery, for each distinct period of operation. Include in the table, percent saturation permitted by the
manufacturer of the RO/NF elements used in the study and for which verification is being sought.

9.0	TASK 4: RO/NF CLEANING

9.1	Introduction

During or following the test runs of Task 2, the RO/NF equipment shall require chemical cleaning to restore
membrane productivity. The number of cleaning efficiency evaluations shall be determined by the fouling
frequency of the RO/NF during each 1,000-hour test period. In the case where the rate of fouling is low
and the decreases in normalized product flow or increases in normalized salt passage do not reach chemical
cleaning criteria as specified by the Manufacturer in Task 1, chemical cleaning shall be performed after each
1,000-hour test of operation, with an evaluation of cleaning efficiency made by subsequent system operation
for a period sufficient to determine cleaning impact.

April 2002

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9.2 Objectives

The objectives of this task are as follows:

•	Evaluate the effectiveness of chemical cleaning for reversing losses in normalized product flow
or increases in normalized salt passage to the RO/NF system.

•	Confirm that Manufacturer- recommended cleaning practices are sufficient to restore membrane
productivity for the systems being considered under the conditions being evaluated.

9.3	Work Plan

The RO/NF systems may become fouled during the RO/NF test runs conducted for Task 2. These fouled
membranes shall be utilized for the cleaning assessments herein. No additional experiments shall be required
to produce fouled membranes; cleaning will only be conducted if fouling causes performance losses to levels
recommended by the Manufacturer and as listed in the PSTP. If losses are not sufficient, cleaning will be
conducted at the conclusion of each 1000-hour test to assess the cleaning efficiency relative to the degree
that such losses were incurred.

Each system shall be chemically cleaned using cleaning equipment (including chemicals) provided by the
Manufacturer and cleaning solutions and procedures specified by the FTO in the PSTP. After each
chemical cleaning of the membranes, the system shall be restarted at test conditions and operated for a
period of 72 hours to monitor response to cleaning of the following productivity indicators:

•	Normalized product flow

•	Normalized salt passage

•	Feed-concentrate differential pressure

Cleaning chemicals and cleaning routines shall be based on the recommendations of the Manufacturer. The
PSTP shall specify in detail the procedure(s) for chemical cleaning of the membranes. At a minimum, the
information in Table 5 shall be provided. In addition, a description of all cleaning equipment and its
operation shall be included in PSTP.

9.4	Analytical Schedule
9.4.1 Sampling

The pH and temperature of each cleaning solution shall be determined and recorded during various
periods of the chemical cleaning procedure, as indicated in Table 5. No other water quality sampling
shall be required.

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9.4.2

Operational Data Collection

RO/NF system performance data shall be collected immediately preceding cleaning and for 72-hours
following return of the system to normal operation (following completion of cleaning). If the
Manufacturer's procedures required cleaning with two separate cleaning formulations, the 72-hour
operating period shall be performed following the completion of the entire cleaning event (final cleanirg
formulation).

9.5 Evaluation Criteria

At the conclusion of each chemical cleaning event and upon return of the RO/NF system to operation,
system operating data (pressure, flow, conductivity, and temperature) shall be recorded four times per day
for a 72-hour period and each performance parameter calculated (normalized product flow, normalized salt
passage, and feed-concentrate differential pressure). The twelve data values for each performance
parameter shall be averaged to obtain a "post-cleaning" value to be used in cleaning efficiency calculations
described in this Task. The efficacy of chemical cleaning for each performance parameter shall be evaluated
as noted below, with comparisons drawn from the cleaning efficacy achieved during previous cleaning
evaluations (where applicable). Comparison between chemical cleanings shall allow evaluation of the
potential for irreversible fouling and projections for usable membrane life.

Two primary measures of cleaning efficiency and restoration of membrane productivity will be examined in
this task:

1)	The immediate recovery of membrane productivity, considering the value of the productivity
indicator at the start of the run, at the end of the run, and after cleaning.

(NPF - NPF )

, TT~. t—i ^	/r\s\ \	Cleaned v Fouled /

NPF Recovery(%) =-,	r

[NPFongrnal " NPF'Fouled)

(NPF - NPF )

NSP Recovery(%) =7	—	^ฃฃ!ฃ4

\NpFFouIed - NPF0riginal J

(AP . , - AP

*	//~\ / \ \ Fouled	Cleaned /

APRecovery(%) =j	f

Fouled ^^Original J

2)	The loss of productivity, considering the value of the productivity indicator at the start of the run
and after cleaning:

f

NPF Loss(%) =

NSP Increase(%)

j NPFqeaned

V NPF Original J

( NKP	^

Cleaned	y

V	NSP0riginai J

(r>p	^

Cleaned	j

v DP	/

V	' y/ Original	J

April 2002

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Table 5

Data to be Recorded for Documentation of Cleaning Efficiency

Parameter(1)

Units

First
Solution

Second
Solution

Notes

Preliminary Flush



Source











Flow rate

gpm









Volume or duration

gal or min







Cleaning chemicals used



Cleaning solution batch volume

gal









Citric acid

lbs









Sodium tripolyphosphate

lbs









Trisodium phosphate

lbs









Sodium EDTA

lbs









Anionic surfactant

mL









Hydrochloric acid

mL









50% Sodium hydroxide

mL









Other:







Other solution components



Other:







List Proprietary cleaning
solution

Solution Recirculation/Soak/Recirculation



pH







Note initial and final



Temperature

deg C





Note initial and final



Initial recirculation period

minutes









Initial recirculation pH











Initial recirculation temperature

deg C









Appearance of solution







Note color, solids, clarity, etc.



Soak period

hours









Final recirculation period

minutes









Final recirculation pH











Final recirculation temperature

deg C









Appearance of solution







Note color, solids, clarity, etc.

Final Flush



Source











pH











Flow rate

gpm









Volume or duration

gal or min







(1) Provide data for each stage if cleaned separately

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10.0 TASK 5: DATA REDUCTION AND PRESENTATION

10.1	Introduction

The data management system used in the verification testing program shall involve the use of computer
spreadsheet software, manual recording methods, or both, for recording operational parameters of the
RO/NF equipment on a daily basis.

10.2	Objectives

The objectives of this task are as follows:

•	Establish a viable structure for the recording and transmission of field testing data such that the
Testing Organization provides sufficient and reliable operational data for verification purposes.

•	Develop a statistical analysis of the data, as described in Test Plans for Equipment Verification
Testing for Physical-Chemical Removal of Nitrate by Ion Exchange and RO/NF Processes.

10.3	Work Plan

The following protocol has been developed for data handling and data verification by the Testing
Organization. Where possible, a Supervisory Control and Data Acquisition (SCADA) system should be
used for automatic entry of testing data into computer databases. Specific parcels of the computer
databases for operational and water quality parameters should then be downloaded by manual importation
into Microsoft Excel (or similar spreadsheet software) as a comma delimited file. These specific database
parcels will be identified based upon discrete time spans and monitoring parameters. In spreadsheet form,
the data will be manipulated into a convenient framework to allow analysis of equipment operation. Backup
of the computer databases to diskette should be performed on a weekly basis at a minimum.

In the case when a SCADA system is not available, field testing operators will record data and calculations
by hand in laboratory notebooks. (Daily measurements will be recorded on specially-prepared data log
sheets as appropriate.) The laboratory notebook will provide carbon copies of each page. The original
notebooks will be stored on-site; the carbon copy sheets will be forwarded to the project engineer of the
Testing Organization at least once per week. This protocol will not only ease referencing the original data,
but offer protection of the original record of results. Operating logs shall include a description of the
RO/NF equipment (description of test runs, names of visitors, description of any problems or issues, etc.);
such descriptions shall be provided in addition to experimental calculations and other items.

The database for the proj ect will be set up in the form of custom-designed spreadsheets. The spreadsheets
will be capable of storing and manipulating each monitored water quality and operational parameter from
each task, each sampling location, and each sampling time. All data from the laboratory notebooks and
data log sheets will be entered into the appropriate spreadsheet. Data entry will be conducted on-site by
the designated field testing operators. All recorded calculations will also be checked at this time. Following
data entry, the spreadsheet will be printed out and the print- out will be checked against the handwritten data

April 2002

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sheet. Any corrections will be noted on the hard-copies and corrected on the screen, and then a corrected
version of the spreadsheet will be printed out. Each step of the verification process will be initialed by the
field testing operator or engineer performing the entry or verification step.

Each experiment (e.g. each test run) will be assigned a run number, which will then be tied to the data from
that experiment through each step of data entry and analysis. As samples are collected and sent to state-
certified or third party- or EPA-accredited laboratories, the data will be tracked by use of the same system
of run numbers. Data from the outside laboratories will be received and reviewed by the field testing
operator. These data will be entered into the data spreadsheets, corrected, and verified in the same manner
as the field data.

11.0	TASK 6: QUALITY ASSURANCE/QUALITY CONTROL

11.1	Introduction

Quality assurance and quality control of the operation of the RO/NF equipment and the measured water
quality parameters shall be maintained during the verification testing program.

11.2	Objectives

The objective of this task is to maintain strict QA/QC methods and procedures during the Equipment
Verification Testing Program. Maintenance of strict QA/QC procedures is important, in that if a question
arises when analyzing or interpreting data collected for a given experiment, it will be possible to verily exact
conditions at the time of testing.

11.3	Work Plan

Equipment flow rates and associated signals should be verified and verification recorded on a routine basis.
A routine daily walk through during operation shall be established to verily that each piece of equipment or
instrumentation is operating properly. Particular care shall be taken to verify that any chemicals are being
fed at the defined flow rate into a flow stream that is operating at the expected flow rate, such that the
chemical concentrations are correct. In-line monitoring equipment such as flow meters, etc. shall be
checked to verify that the readout matches with the actual measurement (i.e. flow rate) and that the signal
being recorded is correct. The items listed are in addition to any specified checks outlined in the analytical
methods.

11.3.1 Daily QA/QC Verifications

•	Chemical feed pump flow rates (verified volumetrically over a specific time period)

•	On-line turbidimeter flow rates (verified volumetrically, if employed).

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11.3.2	Weekly QA/QC Verifications

•	In-line flow meters/rotameters (clean equipment to remove any debris or biological buildup and
verily flow volumetrically to avoid erroneous readings).

•	Recalibration of on-line pH meters and/or conductivity meters, if used.

11.3.3	Quarterly QA/QC Verifications

•	On-line turbidimeters (clean out reservoirs and recalibrate, if employed)

•	Differential pressure transmitters (verily gauge readings and electrical signal using a pressure meter)

•	Tubing (verily good condition of all tubing and connections, replace if necessary)

11.3.4	On-Site Analytical Methods

The analytical methods utilized in this study for on-site monitoring of feedwater and permeate water
quality are described in the section below. Use of either bench-top or on-line field analytical
equipment will be acceptable for the verification testing; however, on-line equipment is recommended
for ease of operation. Use of on-line equipment is also preferable because it reduces the introduction
of error and the variability of analytical results generated by inconsistent sampling techniques.

11.3.4.1	pH. Analyses for pH shall be performed according to Standard Method 4500-H. A
three-point calibration of the pH meter used in this study shall be performed once per day when the
instrument is in use. Certified pH buffers in the expected range shall be used. The pH probe shall be
stored in the appropriate solution defined in the instrument manual.

11.3.4.2	Turbidity. Turbidity analyses shall be performed according to Standard Method 2130
with either an on-line or bench-top turbidimeter. On-line turbidimeters shall be used for measurement
of turbidity in the permeate waters, and either an on-line or bench-top turbidimeter may be used for
measurement of the feedwater (and concentrate where applicable).

The FTO shall be required to document any problems experienced with the monitoring turbidity
instruments, and shall also be required to document any subsequent modifications or enhancements
made to monitoring instruments.

11.3.5	Chemical and Biological Samples Shipped Off-Site for Analysis

Total organic carbon (TOC) and UV absorbance samples shall be collected in glass bottles supplied
by the state-certified or third party- or EPA-accredited laboratory and shipped at 4ฐC to the analytical
laboratory within 8 hours of sampling. The TOC and UV absorbance samples shall be collected and
preserved in accordance with Standard Method 501 OB.

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T:ihlc (t
An:il\(iciil Methods

Parameter

Analysis Type

Standard Methods number
or Other Method Reference

EPA Method'2'

Field

On-Line

Lab

General Water Quality



pH

X

X



4500-H+B

150.1/ 150.2

Total alkalinity

X



X

2320 B



Total Hardness

X



X

2340 C



Calcium Hardness

X



X

3500-CaD



Temperature

X

X



2550 B



Conductivity

X

X

X



120.1

Total Dissolved Solids



X



2540 C



Turbidity

X

X

X

2130 B/ Method 2

180.1

Color

X



X

2120 B<3)



Taste and Odor





X





Inorganic Water Quality



Calcium

X



X

3500-CaD / 3111 B / 3120 B

200.7

Magnesium





X



200.7

Sodium





X

3111B

200.7

Potassium





X



200.7

Ammonia





X



350.3

Strontium





X



200.7

Barium





X

3111D/3113B / 3120 B

200.7/200.8

Iron

X



X

3111D/3113B / 3120 B

200.7/200.8/200.9

Manganese





X

3111D/3113B/3120B

200.7/200.8/200.9

Carbonate, C03





X

Calculation



Bicarbonate, HCOj





X

Calculation



Sulfate





X

4110B/4500-S04= C, D, F

300.0/375.2

Chloride

X

X

X

4110B/4500-d-D

300

Nitrate

X



X

4110B/ 4500-N03-D, F

300.0/353.2

Fluoride





X

4110B/ 4500-F-B, C,D, E

300

Carbon Dioxide





X

6211 M



Hydrogen Sulfide





X



376.1/2

Silica, Si02





X

3120 B / 4500-Si D, E, F

200.7

Organic Water Quality



Total organic carbon





X

5310 C



UV254 absorbance

X



X

5910 B



AOC/BDOC





X

9217



Microbiological



Total coliform





X

9221/9222/9223



Heterotrophic Plate Count





X

9215 B



Notes:

1)	Standard Methods Source: 20th Edition of Standard Methods for the Examination of Water
and Wastewater, 1999, American Water Works Association.

2)	EPA Methods Source: EPA Office of Ground Water and Drinking Water. EPA Methods are
available from the National Technical Information Service (NHS).

3)	Hach Co. modification of SM 2120 measured in spectrophotometer at 455 nm.

1

April 2002

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Inorganic chemical samples, including arsenic, alkalinity, hardness, aluminum, iron, and manganese,
shall be collected and preserved in accordance with Standard Method 3010B, paying particular
attention to the sources of contamination as outlined in Standard Method 30 IOC. The samples should
be refrigerated at approximately 2 to 8ฐC immediately upon collection, shipped in a cooler, and
maintained at a temperature of approximately 2 to 8ฐC. Samples shall be processed for analysis by a
state-certified or third party- or EPA-accredited Laboratory within 24 hours of collection. The
laboratory shall keep the samples at approximately 2 to 8ฐC until initiation of analysis.

Samples for analysis of Total Coliforms (TC) and Heterotrophic Plate Counts (HPC) shall be
collected in bottles supplied by the state-certified or third party- or EPA-accredited laboratory and
shipped with an internal cooler temperature of approximately 2 to 8ฐC to the analytical laboratory.
Samples shall be processed for analysis by the state-certified or third party- or EPA-accredited
laboratory within 24 hours of collection. TC densities will be reported as most probable number per
100 mL (MPN/100 mL) and HPC densities will be reported as colony forming units per milliliter
(cfu/mL),

12.0	OPERATION AND MAINTENANCE

The FTO shall obtain the Manufacturer-supplied Operation and Maintenance (O&M) Manual to evaluate
the instructions and procedures for their applicability during the verification testing period. The following
are recommended criteria for evaluation of Operations and Maintenance (O&M) Manuals for equipment
employing RO/NF treatment.

12.1	Operation

Provide clear and concise recommendations for procedures related to proper operation of the RO/NF
treatment systems and equipment. Include as a minimum, information on the following:

•	Startup

-	Initial startup of system

-	Restart of the system after prolonged shutdown

•	Shutdown and membrane element preservation

-	Short term (less than 48 hours)

-	Intermediate term (48 hours to 1 week)

-	Long Term (more than one week)

•	Chemical Feed Systems

-	Type of chemical to be used

-	Dose rate

-	Automation of chemical control system (e.g., pH control of acid feed)

•	Tolerance of the system to operating conditions

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-	Feed water temperature

-	pH

-	Oxidants (e.g., chlorine)

-	Maximum feed pressure and maximum allowable differential pressure across each
stage

•	Adjustment to operating parameters

-	Product water flux

-	Recovery

12.2 Maintenance

Provide clear and concise procedures for performing maintenance on the system and its components.

•	Explicit instructions for in-situ cleaning of membrane elements

-	Chemicals to be used

-	Guidelines and limits for pH, temperature

-	Procedures for flushing before and after cleaning

-	Recirculation rates and durations

•	Instructions for installing or replacing membrane elements into the system.

•	Recommended or required maintenance schedules for each piece of equipment.

•	A list of spare parts to be kept on hand.

12.2.1 Troubleshooting

•	Provide an explicit list of alarm conditions that will be raised by the system.

-	Pressure

-	Temperature

-	pH

-	Pump Failure

-	Chemical feed low tank level

•	Indicate which alarm conditions will cause automatic system shutdown and provide instructions
for clearing each condition.

•	Provide detailed procedures for verifying integrity of membranes, o-rings, etc. on a vessel-by-
vessel basis.

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13.0 REFERENCES

Brunswick, R.J., Suratt, W.B., and Burke, J.E. "Pilot Testing RO Membranes for Nitrate Removal."
Proceedings of the AWWAMembrane Technology Conference. Reno, Nevada. August 13 -16,1993.

Bilidt, H. (1985). "The Use of Reverse Osmosis for Removal of Nitrate in Drinking Water."
Desalination, 53:225

Dunivin, W., Lange, P.H., Sudak, R.G., Wilf, M. Reclamation of Ground Water Using RO Technology.
Proceedings of the IDA World Conference on Desalination and Water Reuse. Washington, D.C.
August 25 - 29, 1991.

Streeter, V.L. andE.B. Wiley. 1985. Fluid Mechanics, 8th ed. New York, McGrawHill Book Company.

USEPA, (1996). ICR Manual for Bench- and Pilot- Scale Treatment Studies. Office of Ground Water and
Drinking Water, Technical Support Division, Cincinnati, OH.

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CHAPTER 3

EPA/NSF ETV EQUIPMENT VERIFICATION TESTING PLAN
NITRATE CONTAMINANT REMOVAL BY ION EXCHANGE

Prepared By:
NSF International
789 Dixboro Road
Ann Arbor, Michigan 48105

Copyright 2002 NSF International 40CFR35.6450.

Permission is hereby granted to reproduce all or part of this work, subject to
the limitation that users may not sell all or any part of the work and may not
create any derivative work therefrom. Contact ETV Drinking Water Systems
Center Manager at (800) NSF-MARK with any questions regarding authorized
or unauthorized uses of this work.

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TABLE OF CONTENTS

Page

1.0	INTRODUCTION	3-6

1.1	Need for This Verification Testing Plan	3-6

1.2	Manufacturer's Responsibility	3-6

2.0	GENERAL BACKGROUND ON ION EXCHANGE PROCESSES	3-7

2.1	Description of Processes	3-7

2.2	Classification of Ion Exchange Processes Designs	3-9

2.2.1	Fixed Bed Designs	3-9

2.2.1.1	Class 1. Conventional Fixed Bed	3-9

2.2.1.2	Class 2. Up Flow Regeneration Fixed Bed 	3-10

2.2.1.3	Class 3. Fixed Bed With Partial Regeneration and Declassification	3-10

2.2.2	Moving Bed Designs	3-11

2.2.2.1	Class 4. Loop Designs	3-11

2.2.2.2	Class 5. Carousel Designs	3-11

3.0 NSF QUALIFIED TESTING ORGANIZATIONS	3-12

4.0	OVERVIEW OF TESTING PHASES AND TASKS	3-12

4.1	Phase 1. Preparation	3-13

4.1.1 Task 1. Preparation, Coordination, StartUp	3-13

4.2	Phase 2. Field Testing	3-13

4.2.1	Task 2. Initial Plant Characteristics	3-13

4.2.2	Task3. Daily Testing And Data Collection	3-13

4.2.3	Task 4. Cross-Connection and Mechanical Inspection	3-13

4.2.4	Task 5. Evaluation of Secondary Data	3-13

4.2.5	Task 6. Continuous Nitrate Analysis and Monitoring	3-14

4.2.6	Task 7. Quality Assurance and Quality Control	3-14

4.3	Phase 3. Reporting	3-14

4.3.1 Task 8. Data Collection Methods, Management and Reports	3-14

5.0 TESTING SCHEDULE	3-14

6.0	TERMINOLOGY FOR VERIFICATION TESTING AND EVALUATION 3-15

6.1	Perspective on Terminology	3-15

6.2	Terms Defined	3-16

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TABLE OF CONTENTS (continued)

Page

7.0	TASK 1. PREPARATION, COORDINATION AND START UP	3-25

7.1	Introduction	3-25

7.2	Objective	3-25

7.3	Work Plan	3-25

7.4	Schedule	3-27

8.0	TASK 2. INITIAL PLANT CHARACTERIZATION	3-27

8.1	Introduction	3-27

8.2	Objective	3-27

8.3	Work Plan	3-27

8.4	Schedule	3-30

9.0	TASK 3. DAILY TESTING AND DATA COLLECTION	3-30

9.1	Introduction	3-30

9.2	Objective	3-30

9.3	Work Plan	3-30

9.4	Ion Exchange Removal Efficiencies	3-32

9.4.1	Operational Data Collection	3-32

9.4.2	Feedwater Quality Limitations	3-34

10.0	TASK 4. CROSS CONNECTION AND MECHANICAL INSPECTION	3-36

10.1	Introduction	3-36

10.2	Objective	3-36

10.3	Work Plan	3-36

10.4	Schedule	3-37

11.0	TASK 5. OPERATION EVALUATION AND EXAMINATION OF RECORDS 3-37

11.1	Introduction	3-37

11.2	Objective	3-37

11.3	Work Plan	3-37

11.4	Schedule	3-39

12.0	TASK 6. CONTINUOUS NITRATE ANALYSIS AND MONITORING	3-39

12.1	Introduction	3-39

12.2	Objective	3-40

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TABLE OF CONTENTS (continued)

Page

12.3	Work Plan	3-40

12.4	Schedule	3-41

13.0	TASK 7: QUALITY ASSURANCE AND QUALITY CONTROL	3-41

13.1	Introduction	3-41

13.2	Objective	3-42

13.3	Work Plan	3-42

13.3.1	Plant Metering Devices	3-42

13.3.2	On- Site Analytical Methods	3-42

13.3.3	Nitrate Grab Samples	3-42

13.3.4	Continuous Nitrate Sampling and Analysis	3-43

13.3.5	Chloride, Sulfate, Alkalinity	3-43

13.3.6	OIF-Site Analyses	3-43

14.0	TASK 8. DATA COLLECTION METHODS, MANAGEMENT AND

REPORTING	3-43

14.1	Introduction	3-43

14.2	Objective	3-44

14.3	Work Plan	3-44

14.3.1	Manual Methods	3-44

14.3.2	Automatic Methods	3-44

14.3.3	Secondary Data	3-45

14.3.4	Data Interpretation	3-45

14.3.5	Report Preparation	3-45

14.3.6	Report Submission and Comments 	3-46

14.4	Schedule	3-46

15.0	OPERATIONS AND MAINTENANCE	3-46

15.1	Maintenance	3-46

15.1.1	Component Maintenance	3-46

15.1.2	Plant Maintenance	3-47

15.2	Operation	3-47

REFERENCES	3-49

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TABLES

Table 1	Daily Operations Log Sheet for an Ion-Exchange System	3-33

Table 2	Operating and Water Quality Data Frequency for Ion-Exchange Processes	3-35

Table 3	Analytical Methods	3-50

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1.0 INTRODUCTION

1.1	Need For This Verification Testing Plan

This document is the ETV Testing Plan for evaluation of water treatment equipment utilizing the ion
exchange process for nitrate removal. The Safe Drinking Water Act and its state counterparts set standards
for water quality regarding certain contaminants, which are known to occur in public water supplies. The
frequency of testing for these contaminants is also specified. However, the Act does not set standards for
the design, performance, testing or operation of treatment facilities for the regulated contaminants. To a
certain extent, individual States place requirements on some of these areas not covered by the Act. For
example, there are training and certification programs for operators of treatment plants and design reviews
given to proposed treatment facilities followed by a plant operating permit procedure. However, in most
cases, operator training and design reviews are not familiar with the variety of designs, which use the
specialized ion exchange technologies. This ETV Testing Plan provides background information and testing
procedures, which will be of service to owners, operators, state regulators and manufacturers who must
deal with these unfamiliar technical subjects. The responsibility to make effective treatment rests with the
States and the professional disciplines and organizations involved in the effort and in particular on the
equipment designer and supplier or manufacturer of the treatment system.

Under the ETV testing program, it is the manufacturers responsibility to retain a qualified Field Testing
Organization to conduct tests on the plant by following an NSF approved preset testing plan contained in
the Product-Specific Test Plan (PSTP). Other subjects treated in the PSTP are set forth in the ETV
Protocol Document, EPA/NSF ETV Protocol For Equipment Verification Testing For Removal Of
Nitrate: Requirements For All Studies." This Equipment Verification Testing Plan is applicable only to
processes using ion exchange materials as the media in the nitrate removal processes.

1.2	Manufacturer's Responsibility

In order to participate in the equipment verification process for nitrate removal, the equipment Manufacturer
shall retain a qualified Field Testing Organization to employ the terminology, procedures and methods
described in this test plan and in the referenced ETV Protocol Document as guidelines for the development
of PSTP. The testing procedures shall generally follow the tasks that are outlined and described in this
document. An attempt has been made to provide test descriptions to be appropriate to all processes using
ion exchange rather than to a specific design of a process. However, variations in these tasks may be
required to modify or adapt to specific process designs or plant situations. A suggested outline and format
of the procedures written by the Field Testing Organization for each task is given. The outline for each task
will usually contain the following sections:

•	Introduction

•	Objectives

•	Work Plan

•	Schedule

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An overview of the Tasks is given in Section 4. A list of definitions and terms, which are peculiar particular
to nitrate ion exchange, is given in Section 6. The specific Tasks to be included in each PSTP are described
in Sections 7 through 13.

2.0	GENERAL BACKGROUND ON ION EXCHANGE PROCESSES

2.1	Description of Processes

This verification testing plan applies to a wide scope of equipment types which use ion exchange processes.
There is no lack of creativity among ion exchange process designers who have had over fifty years since the
availability of synthetic ion exchange materials to exercise their ingenuity. It is not the intention of a
specifically defined test plan to limit this scope nor to discourage innovation. On the other hand, regardless
of novelty, all ion exchange processes are governed by the same basic ion exchange reactions which
constitute the various processes.

Among various ion exchange materials available for nitrate exchange, synthetic polymeric resins that carry
exchangeable anions are most widely used. Regardless of exchange materials, the nitrate exchange process
can be expressed in two steps, adsorption and regeneration as shown below:

(ion Exchanger )C1 + NO^ —> (ion Exchanger )N03 + Cl(aq) Adsorption Step
(ion Exchanger )N03 + Clbrmcli —> (ion Exchanger )C1 +	Regeneration Step

The Adsorption Step consists of contacting the nitrate-laden water supply with a bed of Ion Exchanger in
the chloride form. The nitrate ion is removed from the water and, in exchange, the chloride ion is added to
the water. The opposite exchange occurs in the Regeneration Step where the Ion Exchanger is restored to
its initial chloride condition where, after rinsing, it can be reused.

If the process were 100 percent efficient, only one chloride ion would be required to remove one nitrate ion
from the water supply. In terms of energy demands, the Adsorption Step occurs with the equilibrium
favoring the product side of the reaction, nitrate ion is much preferred by resins in preference to chloride.
All resins are selective for nitrate over chloride. Therefore, for the Regeneration Step, an energy price must
be paid by using an excess of chloride to drive the reaction to the right.

The nitrate exchange process can be represented by a single overall metathesis or exchange reaction, which
is the sum of two reactions (adsorption and regeneration steps) with a resin giving a net result:

N03(aq) + Cl,hnnc) -> N03(brme) + Cl-aq)	Basic Nitrate Ion Exchange

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Wherein a chloride ion from a concentrated brine replaces a nitrate ion in the untreated water. The net
result is removal of nitrate from the water supply and production of waste nitrate brine. The water softening
process can be represented by a similar net exchange reaction:

CC) + 2Na+brme) -> Canine) + 2Na+q) Ion Exchange Softening

Superficially, the processes appear similar; but they are quite different because of the different
stoicheometries which drastically influence process efficiency. In the nitrate/chloride exchange, the number
of nitrate ions transferred to the brine is directly proportional to CI (brine) according to the law of mass action.
To drive the nitrate reaction to the right, an excess of chloride brine ions is required. However, in the
softening case, the amount of waste calcium ions transferred to the brine is proportional to (Na+ (brine))2
according to the mass action law. In the case of waters of normal hardness, and the usual brine
concentrations, the efficiency is nearly 100 percent, requiring relatively little excess brine. Process designs
for efficient water softening, therefore, are not directly applicable to nitrate removal.

Because the basic process is carried out indirectly through a sequence of steps, efficiency is greatly
dependent on the nature of the ion exchanger and the physical methods used to bring the water supply into
contact with the Ion Exchange medium and then to carry out the regeneration. This situation invites a variety
of process designs each employing specific chemical, hydraulic, and mechanical methods. In the softening
case, both the adsorption and the regeneration reactions favor the right hand side of the two equations
because of the divalent nature of the calcium ion. (This is probably the least understood aspect of
comparisons between nitrate ion exchange and softening). The practical result is that near stoichiometric
quantities of salt regenerant are required in softening, whereas in the nitrate case ten (or higher) to one are
required for a complete regeneration.

The design must bring the water supply into close contact with the Ion Exchanger over a specific time period
and in a three-dimensional uniform flow. Engineers have devised several ways to do this but it is usually
done by placing the Ion Exchanger in a filter bed or columnar arrangement, distributing the untreated water
over the bed and allowing water to flow through at uniform rates; then uniformly collecting the treated water
near the exit of the bed. The same type arrangement is used for the Regeneration Step.

The competition between the major ions present in a water supply for taking over an ion exchange site is
also great enough to be of concern in the design of the equipment and the process. After the Ion Exchanger
is exhausted, the different ions will be concentrated in the resin bed according to the chemical equilibrium
laws of chromatographic distribution. Consequently, the use of different ion exchange resins and different
methods of adsorption and regeneration are employed to overcome any difficulties, which this may cause.
For example, the regenerations will be conducted by flowing the regenerant through the bed in the same or
opposite direction that the water flows in the adsorption step.

Rinsing and wasting the brine from the bed is also performed in different ways and gives rise to different
rinsing efficiencies. In some cases sophisticated designs will reuse at least some of the rinse water, waste
brine, or backwash water. Further variations in process designs are made to ensure that the resin bed is
always in a uniformly packed condition to prevent channeling of fluid through the bed and reducing the

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physical contact time between the resin and the fluid.

In essence, each manufacturer has a large number of variables to deal with in how to accomplish the simple
Basic Ion Exchange process with the particular design and operation of plant equipment. Each may claim
some aspect of the design, which makes it superior to another, or make some claim regarding proprietary or
breakthrough designs.

2.2 Classification of Nitrate Ion Exchange Processes

For the purpose of this verification testing plan the different process designs will be classified according to
common characteristics and expected performance levels. For example, design Class 1 will usually be used
for small units where process efficiency and waste production are of little concern, but high reliability, ease
of operation and water quality objectives are important. In contrast, other design classes may be used
where there is concern about waste disposal and may require more sophisticated regeneration procedures,
adding to the complexity of operation. One cannot, therefore, state that one design is superior to the other,
but only to the extent that the treatment and other related objectives are similar.

The verification testing program will verify manufacturer's objectives regarding the performance of the
equipment. The manufacturer will classify the design according to the following design classifications,
provide flow diagrams of the design and provide projected performance characteristics of the plant.

2.2.1 Fixed Bed Designs

Fixed bed designs employ the ion exchange resin placed in a vessel that is stationary and within
which both the adsorption and regeneration steps are conducted. The contact between resin and
the water (or regenerant) is accomplished by flowing the water (or regenerant) through the
stationary vessel. This is the most common type design. (Some movement of the resin occurs
within the vessel during the backwash, declassification, and rinsing procedures.) The placement and
operation of a number of valves accomplishes the changes in the flow of fluids through the bed in a
fixed bed design.

2.2.1.1 Class 1. Conventional Fixed Bed. This is the simplest type of design and uses the same
equipment and regeneration method that is manufactured for water softener use. Instead of using a
resin for softening water (cation exchange resin) this resin is replaced with an anion exchange resin.
The specific gravity of anion exchange resins is much lower than cation resins. Therefore, the
backwash step and the ion exchange vessel internal components may need to be modified for anion
exchange processes. The bed is run to near exhaustion, then regenerated with excess salt to ensure
regeneration in a down flow direction then rinsed. It is commonly thought, although erroneously,
that nitrate ion exchange differs only from water softening in the kind of resin that is used and the
chemical, physical, hydraulic, and regeneration processes are identical (see above). Although such
designs may remove nitrate and operate reliably, they will also use excessive salt regenerant and
may also produce excessive wastewater. A Class 1 design is likely to be low in capital cost
because no special design considerations for nitrate removal chemistry are included and designs are
made for the softening mass market. Normally, the sequence of steps for one cycle in a Class 1

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design is adsorption, back wash, regeneration and finally, rinse.

2.2.1.2	Class 2. Up Flow Regeneration Fixed Bed This fixed bed design employs regeneration
in an up flow regeneration mode. Distributors within the vessel and valving are designed to
accommodate this design feature. Again, this type of design is primarily used in softening where
very low levels of contaminant ion are required to meet water quality objectives. Problems
encountered are that when brine flows upward through the resin, the distance between resin
particles tends to increase, as the bed tends to expand upward. The result is the regenerant will
channel through the bed with reduced contact with the resin. Designers must somehow compensate
for this problem, e.g., by employing a blocking flow.

The advantage of this design is that the resin at the bottom of the bed has virtually all nitrate
removed so when the bed is placed in service, no nitrate appears in the initial portion of product
water. However, continued production causes nitrate to gradually rise, with a rate dependent on
amount of regenerant used. A second advantage of this design can be realized if the nitrate on the
resin is concentrated at the top of the bed when regeneration starts. Large amounts of nitrate can
be removed from the top of the bed by the regenerant and give good regeneration efficiency. The
amount of nitrate at the top of the unregenerated bed varies with water composition. If sulfate is
present, sulfate concentrates at the top of the bed and this advantage is diminished or can become a
disadvantage. In the latter case, declassification of the resin before regeneration can move some
nitrate to the top of the bed.

In general, a Class 2 design can give a product water very low in nitrate initially. The more nitrate
removed from the treated supply, the more untreated water can be blended in to give an acceptable
nitrate level. This in turn is translated into use of smaller sized vessels and amounts of medium
(lower capital cost). However, greater salt demands are the trade off.

2.2.1.3	Class 3. Fixed Bed With Partial Regeneration And Declassification. This method
was developed by the USEPA for demonstration in McFarland, California, and is employed in
several locations in the U.S. where sulfate is present in the feed water. This design is more complex
than the above systems to minimize the amount of brine and wastewater. The process uses
modified vessel designs with efficient flow distributors and bifurcated collectors. Down flow
adsorption is directly followed with down flow regeneration. This takes advantage of the
concentrated nitrate at the bottom of the bed at the end of the adsorption cycle to give high nitrate
removal efficiency. The bed is only partially regenerated, i.e. large amounts of brine are not used to
remove all nitrate from the bed, only sufficient nitrate is removed to meet water quality objectives.
The next step is to declassify the bed, (accomplished by a series of five uneven back washes via the
bifurcated collector design, to mix the bed) and distribute the nitrate remaining on the bed uniformly
throughout the bed. This is necessary to give a constant level of nitrate in the product water as the
bed is exhausted.

The advantages of this design are more efficient use of brine and less wastewater production than
the above designs especially if sulfate is present.

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2.2.2 Moving Bed Designs

Moving bed designs require that the resin bed (or part of the bed) move from place to place at
some part of the process cycle. An example is to use one vessel to perform the Adsorption Step,
then remove resin and place it in a second vessel where the Regeneration Step is performed. Then
move the regenerated resin back into the first vessel. One of the advantages of this type design is
that less resin inventory is required.

2.2.2.1	Class 4. Loop Designs. These designs are also referred to as moving packed beds or by
different names such as Higgins or Asahi and their variations and have a place in the history of
nitrate treatment, being the first large-scale plant design used in the U.S. at Garden City Park, N. Y.
Their common feature is the movement of resin from one vessel to another for different parts of the
cycle.

In the Higgins Loop reactor, the adsorption occurs in a down flow mode through a first vessel.
Then, the top portion of the bed is moved (pulsed) to a regeneration vessel and regenerated resin is
pulsed back to the bottom of the first vessel. The adsorption and regeneration steps can be
conducted simultaneously but in different vessels. Advantages depend on water quality. On Long
Island, low sulfate water concentrated nitrate at the top of the bed, which is efficiently regenerated
and the down flow mode in a separate vessel avoids the channeling problems encountered in the
fixed bed Class 2 design. Further advantages claimed are that lower resin inventories are required
making capital costs lower. Critics claim low resin life, because of resin attrition caused by pumping
resin slurries from one vessel to another.

The Asahi design is quite complex. Some features are:

1)	up flow adsorption and high flow rates, which pin the bed to the top portion of the
adsorption vessel, while the lower portion of the bed is moved by fluidization to a
regeneration vessel;

2)	after exhaustion the flow stops, the bed falls and draws in regenerated, rinsed resin; and

3)	absorption flow is started again, pinning the fresh resin against the top of the vessel and
moving the spent lower portion to regeneration.

The advantages appear to be the same as the Higgins reactor except resin is moved by fluidization
rather than mechanical pumping.

2.2.2.2	Class 5. Carousel Designs. In this design, the resin bed moves within several vessels
within which resin is contained. The vessels are mounted in "merry-go-round" configuration and
gradually step from position to position by rotation of the entire mechanism. The circular platform
structure contains orifices through which the fluids enter and exit the individual vessels. When the
vessels are in positions e.g. 1 through 10, they are in the adsorption section of the carousel. When
they are in positions e.g. 11 through 15, they are in the regeneration section, and finally when they
are in the last section, the vessels are rinsed. This design has potential to have the highest
regeneration efficiency and lowest wastewater production because various piping arrangements can

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easily recycle brine and rinse water.

3.0 NSF QUALIFIED TESTING ORGANIZATIONS

Testing and evaluation of equipment covered by this Verification Testing Plan will be conducted by a Field
Testing Organization that is qualified by the NSF and selected by the Manufacturer. The water quality
analytical work to be carried out as a part of the Verification Testing Plan will also be contracted by the
manufacturer with a state-certified, third party- or EPA-accredited laboratory.

4.0 OVERVIEW OF PHASES AND TASKS

The PSTP will include a Testing Plan with detailed tasks described and scheduled that will be followed by
the NSF qualified field testing organization. The PSTP plan will be formulated by the Field Testing
Organization to be effective for the particular plant design, operation and field situation. Wide variability in
PSTP plans is anticipated because of these factors. The tasks listed below and detailed throughout this
document are formulated to represent the oontent, vocabulary, organization and quality of testing and
evaluation procedures anticipated by NSF to be included in any PSTP. The Field Testing Organization may
add other tasks. If the tasks listed below are eliminated or substantially modified, a reason for doing so
should be given.

Three phases of testing are to be included in the Verification and Testing Plan.

• The first phase consists of preparation and plant start up. The scope of this phase will depend on
whether or not the equipment has already been installed at a treatment plant site and is already
treating water or if the plant will be delivered to the site and will require set up and start up
procedures.

A meeting of testing personnel with the plant manufacturer will be held to review the material
contained in the PSTP presented by the Field Testing Organization. Much material regarding the
plant and its operation were provided in the PSTP as set forth in the "EPA/NSF ETV Protocol For
Equipment Verification Testing For Removal Of Nitrate: Requirements For All Studies." This
material will include, design classification, drawings and diagrams of the plant design, the start up
and operating procedures. This meeting will allow any questions concerning the plant and the
testing program to be addressed. The manufacturer will confirm the items which will be tested for
during the program and present the objectives concerning operation, make projections of the plant
performance characteristics, and review any critical or key measurements to be made.

The meeting or a part of the meeting should take place at the treatment plant site where various
features and components can be directly pointed out and demonstrated. If the plant is not yet
started, the start up could take place at this time.

*ฆ The second phase is the field testing phase, which will evaluate performance of the equipment over
a 60-day period.

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• The third phase will be conducted throughout the testing period to ensure that the data are
collected in a reliable and retrievable manner, properly and completely reported on a timely basis.
A Data Manager will be responsible for these tasks.

The three phase Testing Plan outlined below is comprised of 8 separate tasks, which are outlined in the
following sections.

4.1	Phase 1. Preparation

4.1.1 Task 1. Preparation, Coordination, Start Up

An orientation meeting will be held in preparation for the testing program. The manufacturer will
meet with the field testing organization personnel to review the plant and process design and clarify
the testing program and schedule. It is recommended that a field visit to the plant be made to
acquaint the testing personnel with details of the plant site. Discussion of the program, its
objectives, and responsibilities of each participant will be clarified. If the plant is not already
operating, the plant will be started approximately 15 days before field tests begin.

4.2	Phase 2. Field Testing

4.2.1	Task 2. Initial Plant Characteristics

Initial tests will be conducted to measure the plant's basic capabilities and characteristics. These
tests will be conducted to produce base line information, which can be used to evaluate changes,
which occur as the plant ages. If the plant does not meet water quality objectives, the Field Testing
Organization will be notified and adjustments made.

4.2.2	Task 3. Daily Testing and Data Collection

Routine measurements and data recording will be conducted on a daily basis for at minimum a 60-
day test period.

4.2.3	Task 4. Cross-Connection And Mechanical Inspection

Two certified or registered professionals will perform inspections of plant equipment and operation:

1)	a cross-connection specialist will inspect and test all cross-connection control and back
flow prevention devices, and

2)	a mechanical engineer will inspect all electrical and irechanical equipment for proper
placement and operation.

4.2.4	Task 5. Evaluation of Secondary Data

Data obtained by the owner/operator the 60-day period during regular plant operation will be
reviewed to evaluate the data collection, management and reporting system of the plant. The
accuracy of the data and information as well as its adequacy will be evaluated. Data found to be

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accurate can be used to supplement the evaluation and testing program.

4.2.5	Task 6. Continuous Nitrate Analysis and Monitoring

In order to evaluate the reliability and stability of the plant operation. Frequent sampling and
analysis of the three different streams (feed, treated and blended) for nitrate levels should be
performed. High frequency sampling (at approximately six-minute intervals) is best done with
modern nitrate measuring instruments. Because of recent improvements and the popular use of
automatic nitrate monitoring instruments at several nitrate plant locations, it is highly feasible to do
close monitoring of nitrate levels in multiple process streams. It is highly desirable that one nitrate
monitor be used to monitor three different process streams over a period of 60 days. If an
automatic monitor is not used, manual samples should be taken from the three different streams as
frequently as feasible for analysis.

4.2.6	Task 7. Quality Assurance and Quality Control

A Quality Assurance and Quality Control (QA/QC) program will be followed to ensure adequate
quality of the data collected.

4.3 Phase 3. Reporting

4.3.1 Task 8. Data Collection Methods, Management and Reports

Data collection, management and reporting will be tasks, which are closely integrated. The contents
of the draft and final report will include specific summaries and items to complete the evaluation of
the plant performance.

5.0 TESTING SCHEDULE

The PSTP will contain a Schedule of Tasks.

Task 1, Preparation, Coordination and Start Up, shall be performed before the plant testing program
begins. The plant shall be operated for at least ten cycles before tests can begin.

Task 2, Initial and Final Plant Characterization, shall be performed at the beginning and end of the 60-day
test program.

Task 3, Daily Testing and Data Collection, typical water quality and operational monitoring shall be
performed at least three times per day and additional data collection shall be performed every two weeks.

Tasks 4, Cross Connection and Electromechanical Inspection, and 5, Operation Evaluation and
Examination of Records, will be done near the end of the 60-day test period.

Task 6, Continuous Nitrate Analysis and Monitoring, shall be at least three times per day over the 60-day
test period

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Tasks 7, QA/QC, and 8, Data Collection Methods, Management, Reporting, will be conducted throughout
the test program.

6.0	TERMINOLOGY FOR VERIFICATION TESTING AND EVALUATION

6.1	Perspective on Terminology

A uniform and consistent terminology shall be used for the evaluation, testing, and reporting of nitrate
systems. This will allow potential users to make direct comparisons between different systems and be able
to choose the most suitable system for their needs. Unfortunately, ion exchange technology is not consistent
and varies with the application of interest. A set of terms will be used that are derived from the Safe
Drinking Water Act (SDWA) itself, from standard chemical terminology, and which are specifically related
to nitrate ion exchange. It is necessary to make distinct and precise definitions because the ion exchange
process, as used for nitrate removal from municipal water, is a relatively new and highly specialized water
treatment method. Ion exchange for nitrate removal is considerably different from older and widely used
technologies of ion exchange for softening or ion exchange for demineralization. Because of these
differences, a different terminology is used to be compatible with the SDWA and to represent the
technological differences between nitrate removal and other applications such as softening. Although this
discussion will appear superfluous to those skilled in ion exchange technology, it is believed to be useful to
those who are not.

For example, the Safe Drinking Water Act specifies Maximum Contaminants Level (MCL) values in
chemical concentration terms of milligrams per liter (mg/L) of the contaminant. Older technology
conventions express concentrations in terms of mg/L of calcium carbonate equivalents or equivalent grains
of calcium carbonate or electrical conductivity. This may be suitable terminology to describe hardness
removal or demineralization, however, for nitrate or any other contaminant, it makes little sense to refer to
nitrate as calcium carbonate or grains of calcium carbonate or conductance equivalents, especially when the
SDWA avoids these terms.

Furthermore, the maj or differences between ion exchange for nitrate removal and ion exchange for softening
relate to the basic chemistry differences between the two processes. It is appropriate to use a different
terminology. The major differences are:

A.	Inefficiencies Due to Presence of Other Ions

Sulfate ion interferes heavily in nitrate removal. Chloride and bicarbonate also interfere. Removal
of calcium ion by softening resins is not as sensitive to these or other cations. Feed water
composition of all major anions is important in nitrate treatment.

B.	Process Efficiency

The nitrate removal process is less efficient than softening. (For example, at typical regeneration
doses of 5 - 10 lbs/cubic foot of resin, the hardness leakage will be close to zero, whereas the
nitrate leakage will be 10 to 20 mg/L depending on water quality.) Designing and operating a plant
for minimum regenerant usage in nitrate removal is critical and process designs different from

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designs for softening are required. Use of salt in the softening process is of less concern because of
the greater efficiency of regeneration. Use of salt in the nitrate removal process is a serious concern
because of the relatively greater quantities of salt required and the cost of disposal.

C. Product Water Quality

In treatment for nitrate removal, processes can be designed to allow some nitrate to pass through
the bed. In softening, only very small amounts of hardness ions are allowed to pass through. To
hold nitrate removal to the same standards of removal as hardness ions can be counter-productive
by increased complexity, lower efficiency and higher cost of the process.

The PSTP will use the following terminology when describing the plant equipment, its operation and
the testing and evaluation procedures. The reports prepared h Task 8 will use also use the
following terminology in describing the evaluation of the plant. This test plan also uses the
terminology presented here.

6.2 Terms Defined

Adsorption - (Same as Ion Exchange Adsorption). The step in the ion exchange process which removes
nitrate from water by chemical or physical attraction to a medium such as an ion exchange resin. It is also
referred to as the SERVICE step or the EXHAUSTION step.

Adsorption Isotherm - A graph showing the amount of material adsorbed as a function of the equilibrium
concentration at a fixed temperature per unit weight of ion exchange material.

Anion - A negatively charged ion. The major anions of concern are nitrate, sulfate, chloride and
bicarbonate.

Anion Exchange Resin - A polymeric material in the form of granular particles or spheres within which
positively charged ionic sites are chemically bound and which can adsorb anions from an aqueous solution
by the ion exchange reaction.

Attrition - Breakage and wear of ion exchange resins.

Back Washing - The upward flow of water through an ion exchange bed to clean it of foreign material and
reduce the compaction of the resin bed. Usually the bed is fluidized by the upward flow of water.

Batch Contact - A method of using ion exchange materials in which the resin and liquid to be treated are
mixed in a vessel and the liquid is decanted off" after equilibrium is attained.

Bead Count - The evaluation of an ion exchange material's physical quality by microscopic evaluation and
determination of percent whole, cracked, and broken beads in a wet sample.

Bed - The ion exchange material contained in a column or vessel of an operating unit.

Bed Depth - The height of the resin material in the column after the exchanger has settled into a packed
bed condition.

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Bed Expansion - The effect produced during back washing: the resin particles become separated and rise
in the column.

Bed Life - The time that a resin bed is allowed to remain in the adsorption step. With flow rate constant,
the bed life equals the number of bed volumes which can be treated divided by the number of bed volumes
treated per unit time.

Bed Volume (BV)- The volume of ion exchange resin material in a bed. The volume of the resin in the bed
is referred to as one bed volume and is expressed in cubic feet, gallons or liters. (The volume of the resin
includes the summation of the volume of each resin particle plus the void volume between the beads.).

Bed Volumes - (or BED VOLUMES TREATED) - A dimensionless ratio that refers to the amount of
water, which can be treated, by a bed of resin. The ratio is volumes of water treated per volume of resin in
the bed.

BreakThrough - The rapid increase in concentration in the effluent of a substance which signals that
adsorption of the substance is near completion and further operation of the column will not be productive.
During plant operation, the adsorption cycle is terminated prior to breakthrough of the ion of interest. (The
breakthrough point can be defined in several different ways such as the point on the breakthrough curve
where the concentration of the ion reaches a given value which is half the value of its feed water value,
halfway between leakage and influent concentration, the MCL or points of inflection. Breakthrough can be
gradual or sharp depending on several factors. Some of these points can be difficult to measure unless
sharp breakthrough occurs. For example, if the influent nitrate is 10 percent over the MCL, the curve has a
flattened or gradual slope when it is at the MCL and the point would be difficult to measure accurately. A
consistent definition should be adopted for any given verification plan.)

Breakthrough Curve - Also referred to as EFFLUENT HISTORY or LEAKAGE CURVE. A curve
showing the relationship between the bed volumes of water passing through a bed of ion exchange resin and
the ionic composition expressed in milliequivalents of the ion per liter in the effluent from the bed over a
range to show sharp or gradual changes in the composition of any ion which denotes its break through the
resin bed. The BREAKTHROUGH POINT on this curve for nitrate ion is only well defined for fully
regenerated resins as the point at which the concentration is one-half of its influent value. The term
EFFLUENT HISTORY is also used in this context and is a more general term which denotes a curve of
leakage over a treatment range, leakage curves may not show clearly defined breakthrough points.

Brine Use Factor (BUF) - A quantitative expression of saltused in practice to remove nitrate from water
by the ion exchange process. The BUF is directly proportional to the salt costs required to operate the
process and is also indicative of the amount of wastewater produced by the process. In theory, if the
process of nitrate removal were 100 percent efficient, the BUF would be 1. The BUF changes with
process and therefor useful in comparing different process designs. Its measured value depends on several
other process parameters such as B V treated, nitrate leakage, salt loading, brine concentration, feed water
composition, resin characteristics, direction of flow through the bed, method of regeneration or brine
recycling etc.

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BUF =

Average number of chemical eqivalents of salt used in regenerati on
Average number of chemical equivalent s of nitrate removed by treatme nt

In practice and in actual plant operation, inefficiencies are experienced because of the inherent chemistry of
the ion exchange process plus the imperfections in process and equipment design. Inefficiencies are
introduced in the process design, the physical equipment, generation of wastewater, and operating
procedures of a plant. The measured BUF is therefore a reflection of the entire plant operation rather than
simply a ratio of two substances. The BUF of an operating system can be estimated by the following
formula when the nitrate concentration is expressed as milligrams of nitrate ion per liter of water. (Not as
milligrams of nitrogen per liter):

BUF =

pounds of NaClused per 1000 gallons of treated water
4.423(untreated mg N/L - treated mg N/L )

127.1

For example, if a plant treats one million gallons of water containing 18 mg-N/L and reduces the nitrate to
4.5 mg-N/L and uses 2000 pounds of salt in the process, the BUF is

BUF =

4.423(18-4.5)

127.1 = 4.26

Capacity - The number of chemical equivalents of exchangeable ion contained in one liter of an ion
exchange material. The volume is measured when the material is wet and is fully saturated with adsorbed
water.

Cation Exchange Resin - A resin to which negatively charged ionic sites are bound and which can adsorb
cations from an aqueous solution.

Channeling - Cleavage and furrowing in the packed resin bed due to faulty operational procedures, or any
condition in which the solution being treated follows the path of least resistance, runs through the channels,
and fails to establish close resin contact.

Chemical Equivalent - The amount of any ion, which contains Avogadro's number of ionic charges. The
chemical equivalent is independent of the weight of an ion. Thus, one chemical equivalent of nitrate is
chemically equivalent to one chemical equivalent of chloride although their equivalent weights differ. SEE
EQUIVALENT WEIGHT.

Chemical Stability - The ability of an ion exchange material to resist changes in its properties when in
contact with aggressive chemical solutions, such as oxidizing agents.

Chromatography - The separation of ions, molecular species, or complexes into highly purified fractions
by means of ion exchange materials or adsorbents.

Clumping - The formation of resin agglomerations in an ion exchange bed due to fouling, chemical
depositions, scaling, or admixture with highly cohesive substances, such as certain clays and silts.

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Column Operation - The most common method of employing ion exchange materials, in which the liquid to
be treated passes through a fixed bed of ion exchange resin held within a cylindrical vessel or column.

Counter Flow Operation - An ion exchange operation in which the direction of flow of water through a
bed and the subsequent regenerant flow are in opposite directions.

Cross-Linking - Binding of the linear polymer chains in the matrix of an ion exchange material with an
agent which produces a three-dimensional insoluble product.

Cycle - A complete series of operational steps. For instance, a complete cycle of nitrate ion exchange
would involve; the complete adsorption step, followed by completion of all other steps and return to the
start of the next adsorption step.

Declassification - A resin mixing operation performed on a resin bed. This is used to evenly distribute the
nitrate adsorbed on a bed of resin to prepare the bed for the following adsorption step. The operation is
performed by using an uneven backwash technique developed at the McFarland, California EPA
demonstration plant.

Degradation - The physical or chemical reduction of ion exchange properties due to type of service,
solution concentration used, heat, or aggressive operating conditions. Some effects are capacity loss,
particle size reduction, excessive swelling, or any combination of the above.

Down Flow - Conventional direction in which water and brines flow through an ion exchange bed during
processing, inlet at the top, outlet at the bottom of the bed or column.

Dumping - Refers to removal of large amounts of nitrate, or any other substance, from an ion exchange
column as detected by its appearance in the effluent in concentrations exceeding its concentration in the feed
water. Nitrate dumping from a resin can occur if sulfate is present in the feed water and if the adsorption
cycle is run beyond nitrate breakthrough. This occurs because sulfate ion is able to displace nitrate from the
downstream portions of the resin column where nitrate is absorbed. Nitrate dumping does not occur if
nitrate selective resins are used or if the concentrations of sulfate and chloride are high such as in
regeneration brines.

Effluent - The solution which emerges from an ion exchange column. Synonymous with PRODUCT or
TREATED water. The regenerant emerging from the column after regeneration is referred to as the
ELUENTorELUATE.

Elution - The stripping of ions or complexes from an ion exchange material by passing through the bed
solutions containing other ions at specific known concentrations.

Empty Bed Contact Time (EBCT)- The time it would take for water to pass through the volume of the
column occupied by the resin bed. It is calculated as though the resin is not present, hence "Empty Bed"
Contact Time. For example if the one Bed Volume is 700 gallons and the flow rate is 350 gal/min, the
EBCT is 2 minutes. Or 0.5 BY per minute.

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Entering Ion - The ion involved in an ion exchange reaction which is adsorbed by the resin and which
displaces a different ion.

Equivalent - See Equivalent Weight.

Equivalent Weight - The sum of the atomic weights in a chemical formula (the formula weight) for an ion
divided by the absolute value of the charge on the ion. This concept is used to compare relative weights of
ions, which can interchange or combine with each other as expressed in a balanced chemical equation. For
example, the equivalent weight of nitrate ion is 62. The equivalent weight of chloride ion is 35.5 and the
weight of sulfate ion is 48. If the weights are expressed in grams, 35.5 grams of chloride ion is chemically
equivalent to 62 grams of nitrate ion (or 48 grams of sulfate ion) in an ion exchange reaction. The equivalent
weight of sodium ion is 23; thus, 23 grams of sodium is combined with 35.5 grams of chloride ion in 58.5
grams of NaCl. MILLIEQIJIVALENT WEIGHT is EQUIVALENT WEIGHT expressed in milligrams of
ion per liter. One equivalent weight of nitrate ion is 62 grams. One milliequivalent weight of nitrate ion is 62
mg/L.

Exhaustion - The state of the resin at the end of the adsorption step and when the capacity of the resin for
adsorbing the ion of interest is used up. The resin is exhausted.

Fouling - Any deposit or concentration of foreign material on or in an ion exchange material which
interferes with the chemical and physical processes. Typical foulants are lubricating oil from pump
lubricants, clays, silts, bacteria, algae etc. Fouling can cause reduced efficiency, channeling, loss of resin in
back wash and many other plant malfunctions.

Freeboard - The space provided above the resin bed in a vessel or column to accommodate the expansion
of the resin bed during the backwash cycle.

Headloss - The loss of liquid pressure head resulting from the passage of water through a bed of ion
exchange material.

Hydraulic Loading Rate - The volume of water passing through a given quantity of resin within a given
time. Flow rate is usually expressed in terms of gallons per minute per square foot of bed cross sectional
area and as gallons per minute per cubic foot of resin. In nitrate treatment these can be 10 to 15 gals/min/sq
ft and 3 to 5 gals/min/cu ft.

Influent - The untreated water entering an ion exchange column.

Interstitial Volume - The space between the particles of an ion exchange material in a column or an
operating unit (see Void Volume).

Leakage - The presence of a substance, usually nitrate, in the treated water exiting from an ion exchange
column before its breakthrough has occurred giving the impression that the substance has "leaked" through
the resin bed. Leakage of nitrate from a resin bed is purposely allowed, but controllable, in all process
designs because it is virtually impossible to regenerate the resin completely. Leakage is different from
Breakthrough.

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Leaving Ion - The ion involved in an ion exchange reaction, which is displaced from the resin by a different
ion.

Milliequivalent Weight - One one thousandth of the amount in one EQUIVALENT WEIGHT. See
EQUIVALENT WEIGHT.

Nitrate Selective Resin same as NITRATE-TO-SULFATE SELECTIVE (NSS) RESIN An ion
exchange resin which will adsorb nitrate ions in preference to sulfate from water. The following
generalizations obtain: All resins are selective for nitrate over chloride, but may not be NITRATE
SELECTIVE. Only special resins (NSS RESINS) are selective for nitrate over sulfate in the range of
drinking water concentrations. Also, all resins are selective for nitrate over sulfate at brine concentrations.

Nitrate Concentration - The units of nitrate concentration in the protocol and verification test plan
documents must be clearly stated and defined as such in the introductory sections. Nitrate will be expressed
as milligrams of the element nitrogen (N) per liter of solution (As opposed to milligrams of N03 per liter).
The conversion factor is 4.423 times mg-N/L = mg- N03/L. California prefers the use of mg- NO3/L as
the expression of nitrate concentration. (Note: The N as the symbol for nitrogen should not be confused
with the N representing solution "Normality" which is the expression of concentration in terms of the
number of chemical equivalents of a substance per liter of solution.)

Operating Cycle - A single completion of all steps in the process consisting of adsorption, regeneration,
rinsing, back wash, stand by.

Osmotic Stability - The ability of an ion exchange material to resist physical degradation due to volume
changes imposed by repeated, alternate application of dilute and concentrated solutions.

Partial Regeneration - The regeneration process which is terminated before all of the ions are removed
from the bed and replaced by regenerating ions. This is practiced in nitrate removal cases because higher
regeneration efficiency can be realized. Regeneration efficiency decreases rapidly with decreasing amounts
of applied regenerant. In practical cases for nitrate removal, even complete regenerations will leave some
nitrate on the bed because of the strong tendency for nitrate to remain attached to the resin. Processes,
which require removal of all nitrate ions (or nearly all), from the bed will require very large amounts of salt
and generate large quantities of wastewater.

Physical Stability - The ability of an ion exchange material to resist breakage caused by mechanical
manipulation.

Presaturant - The ion adsorbed on the resin by saturating the resin with the ion prior to a column
operation. In nitrate treatment the PRESATURANT is chloride ion

Preferred Ion - The one of at least two different ions having equal concentrations that will be adsorbed on
the resin to the greatest extent.

Recontamination - The process of removing a contaminant from one point in a water supply and then
adding the same and/or other contaminant into the supply at a different point. A problem encountered in ion

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exchange systems. For example, by incomplete rinsing of resin beds nitrate, chloride, bicarbonate, sulfate
and sodium can be added to the supply. Also, by running beds beyond their bed life, nitrate ion can be
"dumped" from the bed into the treated water.

Regenerant - The solution used to convert an ion exchange material from its exhausted state to the desired
regenerated form for reuse.

Regeneration - The displacement from the ion exchange material of the ions removed during the
adsorption (service) run. In nitrate treatment, the regeneration is performed by passing a sodium chloride
brine slowly through the bed.

Regeneration Level - The amount of regenerant chemical used per unit volume of ion exchange bed,
commonly expressed as lb/ft3. Also See SALT LOADING.

Resin - Refers to a synthetic ion exchange material. Resins are composed of polymeric water insoluble
organic substances, which have been chemically treated to contain chemically charged ionic sites. In nitrate
treatment, the resin contains quaternary amino groups, each bearing a positive charge. The quaternary
amino groups contain either trimethyl (Type 1), trihydroxyethly (Type 2), or tributyl (NSS, Nitrate
Selective) structures.

Rinse - The passage of water through an ion exchange material to remove excess regenerant. Some rinsing
action also occurs during BACK WASH and DECLASSIFICATION.

Salt Loading - Salt loading is the amount of regenerant applied to a resin during the regeneration step. It
can be expressed in terms of pounds of NaCl per cubic foot of resin, grams of salt/L of resin, equivalents of
salt/L of resin or, more conveniently, in terms of bed volumes of brine (volumes brine/ volumes resin) having
a specified concentration of NaCl.

The latter method allows expression of salt loading as Bed Volumes (BV) of 1 equivalent NaCl/L of brine.
This is equivalent to a salt loading of 3.65 lb. of NaCl/cu. ft. of resin (Derived from 58.5 g/L x 3.781 gal/cu
ft/453.6 g/lb). This method allows a direct comparison to the resin capacity expressed in chemical
equivalents. For example 1.3 B V of regenerant at 1 equivalent of salt per liter will be chemically equivalent
to 1 liter of resin having an exchange capacity of 1.3 equivalents per liter of resin.

The expression of salt loading in terms of BV of brine is a practical consideration. Operation of an ion
exchange plant requires some metering of the salt during the regeneration step. This is conveniently
accomplished by metering the volume of a saturated brine. The amount of salt can be measured from the
volume and brine concentration as determined from specific gravity tables. Salt loading can be expressed in
terms of BV of brine in weight percent NaCl. For example, six percent brine contains 3.901 lb/ft3 and is
slightly more concentrated than a brine containing one equivalent of NaCl/L. Salt loading expressed in BV
of 6% brine is 1.068 (or 3.901/3.65) times greater than salt loading expressed in equivalents NaCl/L.

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Volumetric and Salt Loading Interconversion Factors @ 60ฐF (Note: 1 BY = 1 L)

Volumetric Conversions:

1 Volume of (6% brine) = 1.068 Volumes of (1 equivalent NaCl/L)

Therefore: 1 BV of (6% brine) = 1.068 BV of (1 equivalent NaCl/L)

1 Volume of (1 equivalent NaCl/L) = 0.936 Volumes of (6% brine)

Therefore: 1 BV of (1 equivalent NaCl/L) = 0.936 BV of (6% brine)

1 Volume of (1 equivalent NaCl/L) = 1 Volume of (5.61% brine)

Therefore: 1 BV of (1 equivalent NaCl/L) = 1 BV of (5.61% brine)

Salt Loading Conversions:

1 BV of (1 equivalent NaCl/L) = 3.65 lb NaCl/cu ft resin
1 BV of (1 equivalent NaCl/L) = 58.5 gNaCl/L resin
1 lb NaCl/cu ft resin = 16.03 g NaCl/L resin

Service Run - The step in the operating cycle during which the water is being treated; i.e., nitrate exchange
for chloride. The same as an ADSORPTION RUN.

Set Points - The values of settings, which control the process. These are, Length of Bed Run, Amount of
Brine, Amount of Rinse Water, Amount of Backwash and Declassification, Percent Treated in Blend (see
"Percent Blend" definition on next page). All are set by controller devices reading totalizing flow meters. If
controlled on a time basis, report BOTH time and totalized flow as the setting.

Slow Rinse - That portion of the rinse which follows the regenerant solution and is passed through the ion
exchange material at the same flow rate as the regenerant.

SR-6 Resin - A manufacturer's product identification for a strong base ion exchange resin which has three
butyl groups as part of the quaternary ammonium ions in a styrene divinyl benzene resin. The resin is an
NSS resin and is highly nitrate selective.

Strong Base Resin - A resin that contains quaternary ammonium ions as the functional group in an ion
exchange resin. These groups provide the positive charge sites, which adsorb and hold negatively charged
ions such as nitrate, chloride, and sulfate ions.

Throughput Volume or VOLUME TREATED or BV TREATED- The amount of water passed through
an exchange bed during the service run before.

Type 1 Resin - A strong base resin which has three methyl groups as part of the quaternary ammonium

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ions in a styrene divinyl benzene resin.

Type 2 Resin - A strong base resin which has one hydroxyethyl and two methyl groups as part of the
quaternary ammonium ions in a styrene divinyl benzene resin.

Up Flow - The operation of an ion exchange unit in which solutions are passed in at the bottom and out at
the top of the vessel.

Void Volume - See INTERSTITIAL VOLUME.

Voids - The space between the resinous particles in an ion exchange bed.

The following terms were defined in EPA/NSF ETVProtocol For Equipment Verification Testing For
Removal Of Nitrate: Requirements For All Studies (Chapter 1) and will be used in this testing program.

Financing Cost - The cost to finance the purchase of the equipment based on the rates of inflation,
borrowed capital, and amortization period. To standardize cost calculations, these factors will be set by the
NSF/EPA.

Untreated Water - The raw water that is delivered or available at the site for treatment by the equipment
for nitrate removal.

Treated Water - The water stream that has passed through treatment (and post treatment) and is available
from the equipment either for direct discharge into a distribution system or for blending with untreated water
before inj ection into the water supply system. The PRODUCT WATER is the water that is inj ected into the
distribution system. It contains TREATED WATER and can also contain UNTREATED water if a portion
bypasses the ion exchange vessels.

Blended Water- A mixture of treated and untreated water that is suitable for injection into the distribution
system. This is the same as the distributed water. The blending system may or may not be a part of the
equipment.

Percent Blend - The percent of treated water that is in the blend. Thus a 75 percent blend will refer to
water composed of 75 percent treated and 25 percent untreated water. A 100 percent blended water is
equal to treated water.

Maximum Distribution Flow Rate - The maximum flow rate (gallons per minute) of blended (distributed)
water which the equipment can cause to be injected into the distribution mains on a continuously operating
basis with a nitrate level at or below 80 percent of the MCL.

Maximum Treatment Flow Rate - The maximum flow rate (gallons per minute) of treated water which
the equipment can produce on a continuously operating basis while maintaining the Maximum Distribution
Flow Rate.

Plant Factor - A factor used in computing water treatment cost. It is the fraction of total time the plant

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operates or is projected to operate during its period of amortization. NSF will determine this factor to
standardize cost computations.

Percent Waste - 100 times the ratio of the annual wastewater production to the annual amount of treated
water production.

7.0	TASK 1. PREPARATION, COORDINATION AND START UP

7.1	Introduction

A meeting will be held between the manufacturer and the NSF qualified testing organization regarding the
tasks and scheduling of tasks described in the NSF approved PSTP. This task will also include the plant
start up if it is not already in operation.

7.2	Objective

The objective of the meeting will be to provide an opportunity for the manufacturer and the field testing
personnel to reach a common understanding of the objectives and execution of the testing plan and provide
an opportunity to clarify any areas of concern by either party. Initial start up data will be collected if the
plant is not already in operation. A tour of the test site can be a helpful part of this meeting. Other
personnel associated with the plant should attend if possible, such as the owner/operator and the plant
operator and local or state health officials.

7.3	Work Plan

The following items will be covered in this meeting:

•	The manufacturer will review the material that was included in the PSTP; in particular, the plant
design, operations, outstanding and distinguishing features and especially the treatment objectives
and other secondary performance goals claimed for the plant performance. The treatment
objectives will be reviewed as stated in the PSTP.

The objectives must include the following:

•	The nitrate levels in blended water will be 80 percent of the MCL or less at all times. No nitrate
level should occur higher than this level.

•	Any secondary standard for any other constituent will not be exceeded at any time.

•	Any other objective the manufacturer wishes to include. It is desirable that objectives such as the
following be included.

a)	The BUF will average less than 5.0 during the operation period,

b)	The amount of wastewater produced during the operation of the plant will be less than 1.5
percent of the blended water, or

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c) The Operations and Maintenance (O & M) cost of producing blended water will be under 10
cents per thousand gallons.

The Field Testing Organization will use diagrams, drawings, plans or on site locations to: Point out the
physical limits of the system to be tested, the source water supply, the blending facility and the distribution
lines.

•	Point out the location of the plant control mechanism, pressure gauges, all control valves, their
function, and all instrumentation.

•	Point out the alarm system and alarm/shutdown devices and their functioning.

•	Point out all safety valve and cross-connection control devices and illustrate how they are tested.

•	Walk the testing personnel through the complete operation of the plant, describe the set up and
start up procedures and indicate positions of sampling valves and any automatic data collection and
recording devices. Indicate where set points are set and what their current values are.

The Field Testing Organization will:

•	Review the Schedule for the Testing Plan

•	Prepare the Product- Specific Test Plan

•	Present the Evaluation Criteria. The plant will be evaluated based on its performance regarding the
following:

-	Ability to consistently meet water quality treatment objectives

-	Ability to meet other stated objectives regarding water quality, plant efficiency and
wastewater production etc.

-	Sufficiency of cross-connection devices and their reliability

-	Ability to produce product water with acceptable and constant nitrate levels

-	Material balances for water, nitrate, chloride, and sulfate must be established during plant
operation test periods

-	Wastewater production

-	Plant Efficiency (BUF)

-	Time and effort required for plant set up and start up

-	Operator time and skills required

-	Maintenance time required

-	Quality of parts and construction

-	Reliability of operation.

-	Functioning of safety devices and alarms

-	Reports of plant inspections

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-	Quality of the plants data collection and reporting system.

-	Costs

• Observe and participate in the plant start up procedures. Record the steps of the start up
procedure, note initial set points for the following:

1)	amount of water treated by a single vessel,

2)	amount of brine set for each regeneration,

3)	amount of water used per each rinse,

4)	amount of water used for each backwash, and

5)	percent blend.

The initial set points will be set at the recommendations of the manufacturer.

7.4 Schedule

Before the meeting is held, the Field Testing Organization will provide the Manufacturer with the PSTP
containing the Test Plan and any other drawings, plans, site plans operation manuals and similar helpful
materials. Sufficient time should be allowed prior to the meeting to allow the testing organization to develop
their testing procedure plans and methods to quantify the evaluation criteria. The orientation meeting will be
held immediately prior to the first field test period.

8.0	TASK 2. INITIAL PLANT CHARACTERIZATION

8.1	Introduction

Tests will be conducted to get an initial characterization of the plant and to determine if the water quality
objectives are being met early in the program. These field tests and data collection activities will be
conducted at the start of the testing program to provide a base line for other field tests conducted in Task 3
and at the termination of the test program to see if any operating characteristics change over the test period.

8.2	Objective

The objectives of this task are to establish the initial plant performance characteristics and provide
benchmark data which can be referred to for evaluation of long term changes in plant performance when
future similar data are obtained. The tests can be repeated at intervals throughout the test program if
desired but will be repeated at the end of the test plan.

8.3	Work Plan

At the beginning of the test period, data will be collected from the operating plant, which will characterize
the plant performance (e.g., regeneration level, flow, etc.). These tests will be started only after the beds

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have gone through several cycles at the same settings to allow the plant to reach a steady state of operation.
Steady state will be confirmed from nitrate measurements by achieving a material balance of nitrate
removed from the treated stream and nitrate removed in the waste brine.

From the following chemical analyses, it shall be determined if the water quality performance objectives are
being achieved. If they are not, the manufacturer shall be notified, as they may change the settings on the
plant. If changes in settings are significant, the tests should be suspended to allow the beds to reach a
steady state. It shall be determined if the objectives are being met from the following analyses:

Samples of Feed Water, Treated Water, and Blended Water will be collected for chemical and biological
analyses. The following must be included in the analyses.

•	Total Alkalinity	• Fe	• Sulfate

•	Bicarbonate	• Mn	• Sodium

•	Chloride	• Temperature	• Calcium

All analytical data should be reported as mg/L and equivalents per liter of the ion. The nitrate will be
reported as both mg-N/L and mg-N03"/L.

The following data shall be measured or observed and recorded from the operating plant.

•	Record all set points: Length of Bed Run, Brine, Rinse, Back Wash, Percent Blend, (in units of time,
flow rate and total gallons for each)

•	Number of vessels in service, regeneration, and standby

-	Flow rate of treated water.

-	Flow rate of blended water.

-	Amount of regenerant (pounds of salt) being used for each regeneration.

-	Inspect the plant equipment including piping for any leaks and scale build up.

-	With help from the operator, at least one vessel should be opened and inspected for piping
integrity, dirt, bacterial slime, algae, oil or other foreign material.

-	Estimate the amount of resin in each vessel either by direct inspection or through site glass
observations. Use external vessel ireasurements, corrected for internals and wall thickness.
Record the values of one Bed Volume for each vessel.

-	Take a small sample of the resin for resin tests. See section on resin tests below.

While one vessel is in service, the effluent history curves shall be obtained for each major ion (bicarbonate,
chloride, nitrate, sulfate) from the start of service to its termination. This is done by collecting grab samples
of the treated water at a sample port at or near the exit end of the vessel. (Be sure no other water is
mingled with the treated water at the sample point). Flow meter readings and time of collection of each

•	Nitrate

•	IDS

TOC
HPC
Algae

pH

• Electrical conductivity

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sample or take readings shall be obtained directly from a cumulative flow meter to determine B V of water
treated by the vessel at the time of sample collection. At least 20 samples should be collected. The data
shall be plotted as mg/L of each ion vs. BV of water treated. The data shall be plotted mg-N/L vs. BV
treated.

Any changes in these curves which have occurred since the last measurements shall be noted.

While one vessel is in regeneration, the Field Testing Organization shall:

•	Obtain a sample of the undiluted brine and a sample of diluted brine.

•	Obtain data for a brine elution curve. This is done by taking grab samples at appropriate intervals
of the brine exiting the regenerating vessel. Approximately 20 samples should be taken. Each
sample should be analyzed for the four maj or ions and electrical conductivity. Also from a brine inlet
flow meter, determine the amount of brine entering the vessel. Plot the concentration of each ion
and the electrical conductivity versus the amount of brine entering the vessel.

•	Continue the sample collection after the regeneration from the exit end of the vessel during the rinse
period. These samples should be analyzed for the four major ions and the electrical conductivity.
During the rinse period, record the amount of rinse water used each time a grab sample is taken.
Plot the concentration of each ion and the electrical conductivity versus the amount of rinse water
used.

•	Estimate the amount of sodium chloride added to the water supply as a result of regeneration and
rinsing. This is done as follows: When the above vessel is returned to service, take grab samples of
the product water at one minute intervals for ten minutes to twenty minutes. Analyze each sample
for the four major ions and the electrical conductivity. Plot the concentration of each ion and the
electrical conductivity versus the amount of water treated.

•	During the regeneration and rinsing process, record flow meter readings to determine the total
amount of waste water produced.

•	From the above collected data, make plots (as already mentioned) and calculations as follows:

-	The effluent history curves

-	The brine elution curves

-	The rinsing curves

-	The amount of resin in one BV

-	The amount of salt applied per regeneration in pounds per cubic foot of resin

-	The amount of product water produced per service run

-	The amount of rinse water used

-	The total amount of wastewater (brine, rinse, back wash) produced per vessel per cycle

-	The gallons of saturated brine used per each regeneration

-	The percent blend

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- The percent of wastewater produced

- The brine use factor, BUF

•	From the data collected, determine if the water quality objectives are being met. If they are not,
notify the manufacturer. The set points may require readjustment.

•	The general appearance and condition of the plant will be noted. Note the general appearance of
equipment, new construction etc.

Any changes in this data from the measurements made during the previous test periods shall be noted.

•	Resin Tests. Samples of resin will be regularly tested. These tests may be conducted by the testing
organization or may be submitted to the resin manufacturer who may do such tests on a routine
basis for customers using their products. Tests include, bead integrity or breakage tests done by
counting the number whole and partial beads, capacity determinations, and fouling tests made by
observation of foreign material and chemical analyses.

8.4 Schedule

The above tests will be conducted at the beginning of the field test period after the plant has reached steady
state performance. The tests will be repeated at the end of the field testing period and compared to
previously collected data. The field testing organization should coordinate the timing and nature of the tests
with the plant owner/operator to be sure the plant is being operated and the proper tools are available for
opening the ion exchange vessels for internal inspections.

9.0	TASK 3. DAILY TESTING AND DATA COLLECTION

9.1	Introduction

The plant will be operated and tested on a daily routine basis during the 60-day testing period. Data will be
collected to evaluate operator requirements and activity and reliability of equipment.

9.2	Objective

The objective of routine daily testing is to make close observation of plant operation and to provide
experience and data to evaluate operational characteristics of the plant such as, ease and reliability of
operation, start up and shut down routines, noise production and alarm setting and resetting, reliability of
instruments, flow meters, valve operations and similar day to day operator tasks.

9.3	Work Plan

One or more Daily Plant Data Forms will be prepared to include specific data obtained directly from the
plant's instrumentation, flow meters and gauges. Each manufacturer will provide a sufficient number of flow
meters and instruments for these measurements. Data should be collected daily as described below.

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The data which should be collected daily, at the same time each day, includes the following:

•	Cumulative flow and gpm of source water and cumulative flow and gpm of treated water

•	Cumulative flow and gpm of blended water

•	Cumulative flow and gpm of wastewater

•	Salt Inventory Estimate

For each ion exchange vessel collect the following data:

•	Cumulative flow and gpm of treated water

•	Cumulative flow and gpm of brine

•	Cumulative flow and gpm of rinse water

•	Cumulative flow and gpm of back wash water

Grab samples of raw and finished water streams will be collected once per week for nitrate, chloride,
sodium, sulfate, alkalinity, total hardness, calcium hardness, iron, manganese, color, total organic carbon,
algae, heterotrophic plate count and electrical conductivity. The samples shall be sent to an analytical
laboratory for analysis to reduce cost and increase accuracy. These samples may also be duplicated with
on-site field testing kits for immediate results during treatment process adjustments, particularly with the
rinse water stream. On-site determination of bed exhaustion and regeneration using conductivity meters is
recommended.

Record all instrumentation readings for each stream. Readings will be made each hour from 8 a.m. to 5
p.m. from each vessel.

For each operating ion exchange vessel, the following will be measured and recorded:

•	The number of gallons of treated water produced each time the vessel is in Service Mode.

•	The number of gallons of brine, and its concentration, used each time the vessel is in regeneration
mode as measured at the point of entry into the vessel.

•	The number of gallons of rinse water used each time the vessel is in Rinse Mode.

•	The number of gallons of back wash water and declassification water used each time the vessel is in
Back Wash.

Sufficient data must be collected to enable the calculation of a material balance of total water entering and
leaving the vessel and the total solid regenerant entering and leaving the vessel per each cycle.

Summaries of the data collected during the eight hour period will be made and will include the following:

•	Total daily water treated by the plant

•	Total daily untreated water blended with treated water

•	Total daily blended water sent to distribution

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•	Percent blended water (i.e. Percent of the blended water that is treated.)

Daily summaries for each vessel will include:

•	Number of completed cycles

•	Total daily water treated

•	Total rinse water used

•	Total back wash water used

•	Total pounds of solid regenerant used

•	Total water used to make the regenerant solution

From the above data and chemical analyses, the following will be estimated:

•	Material balances for salt and water

•	Material balances for nitrate, sulfate, bicarbonate, chloride

•	Average untreated water nitrate

•	Average treated water nitrate

•	Average blended water nitrate

•	Amount of salt entering the water supply as a result of incomplete rinsing.

•	Brine Use Factor (BUF). Include both completed and incomplete cycles.

•	Percent wastewater produced (Percentage of the total water supplied for treatment AND
blending that becomes wastewater)

•	Cost of regenerant chemical per 1000 gallons of water distributed
9.4 Ion-Exchange Removal Efficiencies

9.4.1 Operational Data Collection

Removal efficiencies of nitrates from raw water will be assessed by the percentage of removal from the
source water. Measurement of influent raw water flow and pressure and finished water flow and
pressure shall be collected each hour from 8 a.m. to 5 p.m. per day from each vessel. Table 1 is an
example of on-line readings for a daily operational data sheet for an ion-exchange system for 3 shift
readings. This table is presented for informational purposes only. The actual forms will be submitted as
part of the test plan and may be site-specific.

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TABLE 1: Daily Operations Log Sheet for an Ion-Exchange System

Date:

Parameter

Shift 1

Shift 2

Shift 3

Time







Initial







Raw Water







Qraw (gpm)







Nitrate (before pretreatment) (mg/L)







Nitrate (after pretreatment) (mg/L)







TDS (mg/L)/ Conductivity (|amhos/cm),
(before pretreatment)







TDS (mg/L)/ Conductivity (|amhos/cm),
(after pretreatment)







Praw (PSI)







pHraw (before pretreatment)







pHraw (after pretreatment)







T raw (ฐC)







Ion-exchange Vessel







Q (gpm)







Nitrate (mg/L)







TDS (mg/L)/ Conductivity (|amhos/cm)







P (psi)







Finished







Qfm (gpm)







Nitrate (mg/L)







TDS (mg/L)/ Conductivity (|amhos/cm)







Regeneration (@ what % brine or NaCl)







Qregen (gpm)







TDS (mg/L)/ Conductivity (|amhos/cm)







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Table 2 presents operational sampling and sample frequency for on-line, field and laboratory analysis.
On-line meters and probes should be available for instantaneous recordings. Water quality should be
analyzed prior to start-up and then every two weeks for the parameters identified in Table 2 at a
certified laboratory, except for nitrates, which will be monitored prior to start-up and then weekly.
Field sampling should be performed weekly, if samples can not be analyzed in the field then they should
be sent to the certified laboratory once a week. Power costs for operation of the ion-exchange
equipment (pumping requirements, chemical usage, etc.) shall also be closely monitored and recorded
by the FTO during the 60-day testing period. Power usage shall be estimated by inclusion of the
following details regarding equipment operation requirements:

•	pumping requirements;

•	size of pumps;

•	name-plate;

•	voltage;

•	current draw;

•	power factor;

•	peak usage; etc.

In addition, measurement of power consumption, chemical consumption shall be quantified by recording
day tank concentration, daily volume consumption and unit cost of chemicals.

9.4.2 Feedwater Quality Limitations

The characteristics of raw waters used during the 60-day testing period (and any additional 60-day
testing periods) shall be explicitly stated in reporting the removal data for each period. Accurate
reporting of such raw water characteristics is critical for the Verification Testing Program, as these
parameters can substantially influence the range of ion-exchange performance and treated water quality
under variable raw water quality conditions.

•	Evaluation criteria and minimum reporting requirements.

•	Plot graph of raw and finished nitrate concentrations over time for each 60- day test period.

•	Plot graph of removal of nitrate over time for each 60-day test period.

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TABLE 2: Operating and Water Quality I

>ata Frequency for Ion-Exchange Processes

Parameter

Frequency

Raw Water Flow

Daily

Finished Water Flow

Daily

Regenerant Flow

Daily

Raw Water Pressure

Daily

Finished Water Pressure

Daily

Regenerant Pressure

Daily

List Each Chemical Used, And Dosage

Daily Data Or Monthly Average

Hours Operated Per Day

Daily

Hours Operator Present Per Day

Monthly Average

Power Costs (kWh/Million Gallons)

Monthly

Independent check on rates of flow

Weekly

Independent check on pressure gages

Weekly

Verification of chemical dosages

Monthly

Feed Water and Finished Water
Characteristics

On-line

Field

Lab

Nitrate

Continuous

Daily

Weekly

Temperature

Continuous

Daily

...

pH

Continuous

Daily

...

TDS/Conductivity

Continuous

Daily

Weekly

Chloride

—

Weekly

Weekly

Sulfate

—

Weekly

Weekly

Sodium

—

Weekly

Weekly

Total Hardness

—

Weekly

Weekly

Calcium Hardness

—

Weekly

Weekly

Total Alkalinity

—

Weekly

Weekly

Iron

—

Weekly

Weekly

Manganese

—

Weekly

Weekly

True Color

—

Weekly

Weekly

Total Organic Carbon

—

...

Weekly

Algae, number and species

—

...

Weekly

Heterotrophic Plate Count

...

...

Weekly

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10.0

TASK 4. CROSS-CONNECTION AND MECHANICAL INSPECTION

10.1

Introduction

Professionals will participate in the evaluation and testing program. Testing and inspection of all of the
cross-connection prevention devices will be conducted. A professional will also inspect and test all meters,
gauges, valves, instrumentation, motors, compressors and similar devices for proper placement and
functioning.

The objective is to evaluate mechanical, safety and qualitative features of the plant including cross-
connection control devices, quality of components, to test cross-connection prevention devices and
operation. Assistance of a registered mechanical engineer and a certified cross-connection specialist will be
required.

A Physical Testing and Inspection Schedule and Checklist will be prepared. The checklist will contain the
location of all cross-connection prevention devices, air gaps, check valves, block and bleed valves, back
flow preventers and similar devices and all electrical and mechanical equipment. The testing will be
performed over a time interval to evaluate effects of long term usage.

A site visit by a person certified for inspection and testing of cross-connection prevention devices will be
made. A separate report will be prepared by the certified inspector after completing the two following
steps.

•	All cross-connection prevention devices included in the plant and blending system will be
inspected and tested. A piping plan will be supplied and all such devices will be indicated for easy
location. The detectors to set off alarms or indicators in event of failure of these devices will also be
inspected and tested by simulating failure conditions.

•	The piping plan will also be inspected to locate potential trouble spots, where valve leakages,
loss of pressure or back siphoning could result in the transfer of waste nitrate and waste salt into the
drinking water supply and where safety would be enhanced by providing additional safety devices.

An engineer with specific experience in dealing with ion exchange field equipment will inspect the brine
pumps, valves, controllers, compressors, motors, probes, gauges, flow meters, alarms, level controllers,
electronic controllers, vessels, storage tanks and similar items on the checklist of mechanical electrical
equipment for functional operability, wear, leaks, corrosion, scale build up, and any damages and general
suitability. The accuracy and range of gauges and flow meters should be tested or estimated from
experience and manufacturer's literature. The performance of valves should be inspected and valve
manufacturer data sheets critically reviewed to determine if sufficient closure and sealing is accomplished for
the application and for low maintenance operation. The valve type should be noted and if the location and
function is appropriate for the type of valve being used. Particular attention should be paid to devices which

10.2 Objective

10.3

Work Plan

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are subject to corrosion and wear such as brine pumps, other brine system parts and waste brine pumps
and storage tanks. All findings will be included in a special reports prepared by the certified mechanical
engineer listing those items inspected and an assessment of the condition and an estimate of the lifetime of
the item if current usage is extended. The reports should include any specific items, which would require
high maintenance, or be particularly costly. The reports will also include an estimate of annual maintenance
cost to maintain, service and replace all mechanical and electrical items to allow the plant to operate without
interruption.

10.4 Schedule

As stated above, two inspections will be performed; the first inspection will occur at the start of the testing
program and the second inspection will be performed after the plant has been in operation for approximately
one year. Each inspector will prepare reports after the first inspection and after the second inspection.

11.0 TASK 5. OPERATION EVALUATION AND EXAMINATION OF RECORDS
11.1. Introduction

The direct collection of field data by the Field Testing Organization is considered in this test plan as
PRIMARY data. Data collected by others and provided to the testing organization is considered to be
SECONDARY data. This task deals with the latter. This other data can be collected by the
owner/operator as a part of the regular operating procedures of the plant. This data can be valuable in the
evaluation testing program to show consistency of plant performance and to fill in any data gaps which may
occur during the brief test periods of primary data collection.

11.2	Objective

The objective is to make an evaluation of the reliability and accuracy of the plant's data collection,
management and reporting system used in the regular or routine plant operation and maintenance. If the
data collection are found accurate, they can supplement the primary data obtained by the NSF Qualified
Testing Organization and will support the evaluations made using primary data. These data can be valuable
in the evaluation testing program to show consistency of plant performance and any data missed related to
rare incidences of operating and maintenance problems. The secondary data will be obtained with
cooperation of the owner/operator from the daily operating logs and records kept by the owner/operator
and other reports on plant operation.

11.3	Work Plan

Preparation. At the beginning of the testing program, the testing organization personnel will meet with the
plant owner/operator and review the daily or weekly plant operating records and logs used for normal plant
operation. The frequency, type, location of recorded data (notebooks, instrument recordings, lab reports
etc) should be noted. The records should include the data listed below for easy recall. If appropriate and
agreeable, the owner/operator may be requested to provide data on special forms or modem transferable

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computer files provided by the testing organization.

Secondary Data. The data which should be reviewed will be the following:

•	Daily and Weekly data:

-	Total gallons of blended water delivered into the distribution system

-	Total gallons of water treated for nitrate removal

-	Total untreated gallons blended with treated water

-	Percent treated in blended water

-	Total gallons of saturated brine used in the regenerations

-	Total pounds of salt used

-	Total number of regenerations performed

-	Total amount of wastewater produced

-	Percent of water delivered to plant for treatment and blending that becomes
wastewater.

•	Daily and Weekly records of:

-	Nitrate in treated water

-	Nitrate in untreated water

-	Nitrate in blended water

-	Electrical conductivity of treated water

-	Equipment and parts deliveries

-	Salt deliveries to the plant

•	Number of occurrences of the following will be recorded:

-	Nitrate levels in distributed water exceeding 80 percent of the MCL value

-	Electrical conductivity of rinse water exceeding normal levels

-	Alarms and their cause

-	Normal maintenance procedures

-	Unscheduled maintenance activity

-	Unscheduled plant shut downs and their causes

Use of Secondary Data. The above data will be reviewed by the testing organization and a Summary of
Secondary Data will be prepared. The following will be estimated on a daily or monthly basis from the
Secondary Data:

• Material balances for salt and water

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•	Average untreated water nitrate

•	Average treated water nitrate

•	Average blended water nitrate

•	Amount of salt entering the water supply as a result of incomplete rinsing

•	Brine Use Factor (BUF). Include both completed and incomplete cycles

•	Percent wastewater produced. (Percentage of the total water supplied for treatment AND
blending that becomes the wasted water.)

•	Cost of regenerant chemical per 1000 gallons of water distributed

•	Agreement or disagreement with primary data and conclusions based on primary data

An evaluation of the plant's data collection, recording, management and reporting system will be made by
comparing the data with that obtained by the NSF Qualified Testing Organization. A general evaluation of
the data collection and reporting system should also be made from the standpoint of accuracy, sufficiency,
and reliability. Is the system giving enough data to tell if the manufacturer's performance objectives are
being met? Is too much data being obtained and is the interpretation confusing? Are alarms recorded? Is
there enough and accurate data for the operator to determine if the plant is operating efficiently? Is the data
recall system reliable and is the data easy to find and well organized?

11.4 Schedule

The secondary data for this task will be the normal operating data collected by the owner/operator
throughout the 60-day test period concurrent with the test program. The secondary data will be made
available to the testing organization on a weekly basis. If additional secondary data are required, the testing
organization will make a request for it at the initial meeting.

12.0	TASK 6. CONTINUOUS NITRATE ANALYSIS AND MONITORING

12.1	Introduction

Consistency, reliability and stability of the operation of an ion exchange plant to produce water of
acceptable quality should be tested on a continuous basis, during a 60 day uninterrupted period in the testing
program. Consistency and reliability relate to the long-term operation, while stability relates to absence of
spiking and fluctuations which may occur during a relatively short term and which may cause plant
disruptions or maladjustments. Nitrate analysis by occasional grab samples say one or two per day, is not
frequent enough to detect fluctuations which may occur in the product water or other streams.

Fluctuations or swings in nitrate levels may be expected to some extent because of the cyclic nature of the
ion exchange processing. When a resin bed is placed into service, the water quality may change throughout
the bed run up to the regeneration. Any given bed may undergo up to about five regenerations per day.
For example, after regeneration, some nitrate remains on the bed. If the bed is improperly rinsed or

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declassified, high nitrate spikes can occur at the start of the run. Also, if nitrate increases during the run, or
the end of run set point malfunctions or is set too long, an over run may occur which is the cause of
"dumping" high nitrate into the product water. Malfunction of equipment during regeneration or rinsing can
also cause high nitrate levels to rapidly rise in the product when the vessel is returned to service.
Malfunctioning, slow operating, or stuck valves may also cause similar problems.

If the fluctuations become extreme the plant is described as "unstable" and can be the cause of two major
problems.

1)	The water quality standard and treatment objective may be violated, and

2)	The plant may be thrown into an upset, which could cause serious water supply and water quality
violations.

These upsets would be caused by set point changes which either the instrumentation or the operator is
basing on grab sample analyses, which are not typical or average. For example, if a high nitrate analytical
result is obtained, an automatic instrument may cause a vessel to go offline and regenerate prematurely.
This in turn can cause a water supply failure and an excessive use of salt.

The above plant malfunctions can best be detected by doing continuous, on stream analysis. Strictly
speaking, the best and most reliable analyzers can only do grab samples in rapid succession. Ion
chromatography instruments or instruments using ultraviolet detectors can make analyses at a frequency of
about 12 analyses per hour. One or more of these instruments should be employed in this testing
procedure.

12.2	Objective

The objective is to test the consistency, reliability and stability of the plant to produce water meeting the
water quality nitrate objectives. This will be done by monitoring the nitrate levels in three different streams
and one monitoring stream by using automatic, continuous, on site, 24 hour per day sampling and analysis
for a period of 60 days.

12.3	Work Plan

Preplanning: The most active season of nitrate plant operation should be determined prior to the start of the
testing program. Some plants operate only during summer periods of high water demand others are most
active during the growing season when fertilizers are used. A site plan will be prepared to show, housing,
location of equipment, benches, sinks, sample taps, sample lines, electrical power and drainage. Location
of computer and/or recording instruments will be shown. If modems are used, show locations of telephone
access lines. More than one analyzer may be needed.

Set Up: Allow one month for set up and testing the analyzer. The nitrate analyzer will be set up in an
enclosed area at the plant site and separate sample lines will be connected to supplies of treated, untreated,
blended, and a monitor supply, for delivery to the analyzer. The monitor supply will be provided from a
batch container and will be prepared from chemical standards having a nitrate composition of 10 mg N/L
and the approximate composition of bicarbonate, chloride and sulfate in the product water. Flow of sample

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will be regulated or delivered via timed sample pumps to prevent sample contamination from other water
sources. A calibration standard will be used as recommended by the manufacturer of the analyzer.

Operation of Analyzers: Continuous nitrate analyses will be performed for a period of 60 days on each of
the three streams of blended water, treated water and untreated water. The analyzers will be programmed
to sample each stream in succession. A sample from the monitor solution will also be analyzed to allow
corrections to the nitrate data to be made.

Automatic analyzers usually have provisions for chart recording or serial ports for transfer to computer files.
The latter can be stored in the computer and also delivered to remote locations vial modem transfers. The
latter would have the advantage of low cost data management and collection by the testing organization.

Data Interpretation: The data collected on the three different streams can be rapidly and easily examined in
the form of computer data files and spreadsheet graphics. The following data should be obtained:

•	Total number of nitrate analyses obtained from each stream

•	Average nitrate level obtained over each 24 hour period, each week, and for 60 days

•	Standard deviation for each stream

•	Number and frequency of exceeding water quality objectives

•	Number and frequency of nitrate spikes

•	Duration of nitrate spikes

•	Relation of spikes to daily time

•	Relation to diurnal temperature as obtained from local weather data

•	Relation to vessel cycles as recorded by plant operator

•	Relation to operator activities as reported by operator

•	Relation to maintenance operation as reported by operator

•	Relation to water production as reported by operator

12.4 Schedule

Task 6 will be conducted over a continuous 60 day period which can coincide with other scheduled tasks.

13.0	TASK 7: QUALITY ASSURANCE AND QUALITY CONTROL

13.1	Introduction

Quality assurance and quality control of the measured water quality parameters shall be maintained during
the Verification Testing program.

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13.2 Objective

The objective of this task is to maintain strict QA/QC methods and procedures during the Equipment
Verification Testing Program to provide accurate data from which reliable conclusions and evaluations can
be made. Maintenance of strict QA/QC procedures and records is important, in that if a question arises
when analyzing or interpreting data collected for a given test or experiment, it will be possible to verify exact
conditions at the time of testing through procedure and record recall.

13.3 Work Plan

13.3.1	Plant Metering Devices

Metering devices, flow meters, pressure gauges, thermometers, sensors, analyzers, probes, and
associated electronic signals should be inspected and verified to be working or not on a routine
basis. A daily walk-through during testing will be established to verify that each piece of equipment
or instrumentation is operating properly. In-line monitoring equipment such as flow meters, etc. will
be checked to verify that the readout matches with other meters registering the same flow stream
and that the signal being recorded is correct. Accurately calibrated flow meters may be attached to
the plumbing to aid calibration.

Accuracy of readings need be verified at least once per week.

13.3.2	On-Site Analytical Methods

Use of portable field test equipment will be used unless regular laboratory wet chemical capabilities
are available for the on-site measurements. Accuracy of calibrated field kits should be determined
by comparison with split sample analysis by a certified lab. For all sample collections, preservation
and storage for later analysis and the analyses themselves should be done according to recognized
procedures listed as acceptable for drinking water in Standard Methods or EPA Methods.

13.3.3	Nitrate Grab Samples

Nitrate analyses will be the most frequently performed analysis. It is very convenient to use the field
test kits commercially available for drinking water samples such as Hach NI-11 and similar kits. A
variety of kit types are available ranging in price and sophistication. The procedures for using the
kits should be carefully followed. In addition, it has been found that results can vary with the
individual preferences and practices. Colorimetric comparators are particularly troublesome. The
nitrate measurements should be made by one person who has practice and proven competence
with the method.

If a color comparator using a color graduated circular disk is used the following precautions should
be made.

1) Read against light reflected from a white panel lit by a good white light dispersed light
source such as a fluorescent bulb.

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2)	Never use sky light or sunlight.

3)	Use the same light source and position for all readings.

4)	Calibrate against known standards.

5)	Make a new calibration if the analyst changes, the light source changes, the color wheel
changes, at least once per week in any case.

In any case, regardless of the kit used, a calibration should be performed against known standards
once per week and every tenth sample should be a sample of known concentrations.

13.3.4	Continuous Nitrate Sampling and Analysis

The use of continuous sampling nitrate analyzers was discussed under a separate section of this test
plan. These instruments can be programmed for calibration at set intervals. The use of a monitor
solution is also recommended to save calibration solution and time. The monitor solution should be
a batch source of known concentration containing representative concentrations of the other major
ions. Corrected readings will be based on both the monitor readings and the standard solution
readings.

13.3.5	Chloride, Sulfate, Alkalinity

Laboratory and field test kits are also available for doing analyses for these ions. The kit selected
should use methods listed in Standard Methods. Each method should be field calibrated over a
range of concentrations available in the various streams subject to analysis. Split samples should
also be submitted to a state certified laboratory for checking accuracy of the field kit analysis.

13.3.6	Off-Site Analyses

Inorganic chemical samples shall be collected and preserved in accordance with Standard Method
3010B. The samples should be refrigerated at approximately 2 to 8ฐC immediately upon
collection, shipped in a cooler, and maintained at a temperature of approximately 2 to 8 ฐC.
Samples shall be processed for analysis by a state-certified, third party accredited or EPA
accredited laboratory within 24 hours of collection. The laboratory shall keep the samples at
approximately 2 to 8ฐC until initiation of analysis.

14.0	TASK 8. DATA COLLECTION METHODS, MANAGEMENT AND REPORTING

14.1	Introduction

It is important to give considerable thought prior to the start of any data collection regarding the collection
methods and management of data. Data management and the reporting and evaluation of the data should be
integrated with each other because of the limited time allowed for the testing plan.

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The data collection methods used in the verification testing program shall involve the use of manual field note
books, logs, field forms and/or computer files of software programs. Any software used must be
compatible with NSF software preferences. It is particularly important that data from the continuous nitrate
analysis instrumentation be kept on computer files, as this provides a convenient method of collection,
transfer, storage and evaluation.

It should be considered from the outset, that the method of data collection and management employed by
the plant owner/operator was designed in response to plant operation and regulatory reporting and should
not be a primary source of the data required by the testing plan. Plant testing is not an objective of plant
operation. The primary objective is to provide safe drinking water to the public. Consequently, it is
preferred that the field testing organization remain as independent as possible from the data system adopted
by the owner/operator. The data collection and management system used for daily plant operation is itself a
part of the plant and is evaluated in Task 5 above. Valid testing should therefore be done using independent
methods of data collection and management.

14.2	Objective

The objective of this task is to implement methods of data collection, management, and evaluation which are
consistent with the testing plan and prepare the final report product.

14.3	Work Plan

14.3.1	Manual Methods

The field testing organization will prepare data forms or log forms to be used by their personnel to
collect the data required for each of the specific tasks as described above. One individual, the Data
Manager, will be assigned the data management task. All original completed data forms and logs
will be submitted to the Data Manager for filing and transfer. The Data Manager will be an engineer
or scientist experienced in water treatment and testing and will be closely involved in the testing plan
on a daily basis. The Data Manager will compile the manually obtained data into computer files for
use in spreadsheets or other data management software and retained for data evaluation. The Data
Manager will also review data and make evaluations regarding water quality objectives and plant
characteristics based on current data. The Data Manager will also organize the data according to
the outlines of the quarterly and final reports and be active in the preparation of the reports.

14.3.2	Automatic Methods

Data from automatic recording devices may be transferred to electronic files for storage and recall
either with field computers or by remote transfer to the Data Manager for the project. If available,
the Data Manager shall download the files once a day during automatic data collection periods.
The data from the automatic nitrate analyses is an example of this application.

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14.3.3	Secondary Data

The data collected by the plant owner/operator is considered as secondary data in this testing plan.
The Data Manager will coordinate the collection of secondary data with the owner/operator.

14.3.4	Data Interpretation

The data will be interpreted in light of the water quality objectives and other operational objectives
to verily the plant performance.

14.3.5	Report Preparation

The report will be organized by task number plus other sections appropriate for the 60-day test
period and will contain a description of all data obtained during the test program. The report will
include an executive summary, data summaries, data charts, data tables, and conclusions, which can
be drawn concerning the plant performance and the achievement of performance objectives.

The final report will contain an Executive Summary that will include the following information given
in brief tabular form:

•	Plant Identification:

-	Design classification of the equipment

-	Location of the test site

-	Maximum flow rate, g.p.m.

-	Duration and dates of testing period

•	Achievement of Objectives:

-	Whether or not the water quality and secondary objectives were achieved

-	Cases, if any, where the plant failed to meet objectives

•	Performance Characteristics:

-	The amount of salt used per day, per month, per year

-	The amount of salt used to produce blended water, pounds per 1000 and million
gallons

-	The average BUF of the operating plant

-	The cost of regenerant per 1000 gallons of blended water

-	Percent of wastewater produced

-	Instances of operational problems or difficulties

-	Evaluation of equipment quality, construction and service

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-	Evaluation results of cross-connection devices

-	Number of shut down alarms during operation

-	Maintenance level required

-	Overall rating and evaluation

•	Estimated Cost:

-	Annual maintenance cost

-	Annual operating cost including capital amortization

•	Projections:

-	The performance characteristics of the plant and costs will be projected for various
representative compositions of feed water with (1) greater and (2)lesser amounts of
each of the four major anions.

14.3.6 Report Submission and Comments

A draft final report will be submitted to the manufacturer for comments. The final report will
address the comments of the manufacturer.

14.4 Schedule

The data collection methods and data management will be in effect throughout the test program. A draft
final report will be submitted after the 60-day test period followed by a final report.

15.0	OPERATIONS AND MAINTENANCE

The following are recommendations for criteria for O&M Manuals for ion exchange equipment and should
be evaluated by the Field Testing Organization during verification testing.

15.1	Maintenance

15.1.1 Component Maintenance

The maintenance section will include references to equipment manuals, which describe maintenance
procedures and calibration procedures. Refer to manuals prepared by each manufacturer of each
component, which requires maintenance. The manufacturer's manuals must be provided in an
appendix. Summarize the maintenance and calibration procedures, which are recommended by the
manufacturer of each of the major components, valves, meters, instruments and controllers.

Provide a Maintenance and Calibration Schedule or Table indicating recommended frequency of
maintenance and the specific maintenance activity for each maintained component.

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15.1.2 Plant Maintenance

The following items must be addressed regarding general plant maintenance:

•	Testing resin samples for capacity and breakage.

•	Inspection and cleaning of vessel interiors and ion exchange resin.

•	Inspection of all piping connections for leakage.

•	Keeping the plant area clean and free of debris.

•	Inspection and renewal of painted surfaces such as vessels, piping, housings, etc.

•	Inspection of sample lines for free flow samples.

•	Inspection of all valves for leakages.

•	Inspection and maintenance of all back flow preventors and cross connection devices for
leakages and/or malfunctioning.

•	Inspection and maintenance of air pressure throughout the system for automatic valves.

•	Inspection of all electrical cables, voltages, and heat production areas and devices.

•	Inspection of brine pumps and brine leakages in brine maker area.

•	Inspection of fire extinguishers, air packs, showers, eye washes and other safety devices.

•	Inspection of controller cables, cabinet interiors, relays, heat generation and functioning.

•	Inspection and routine tests of computer components, printers, modems, etc.

•	Supply of salt for brine making.

•	Chemical supplies for analytical devices and procedures.

•	Supply of analytical sampling bottles.

•	Supply of recording paper and computer printer paper.

•	Any other items required for proper plant operation.

15.2 Operation

The operation manual section should be prepared by a person who has field experience and has actually
operated the equipment and can give a clear description of theory and practice of operation. Input from
design engineers or other office-confined personnel should be minimized. The material must be presented
from the plant operator's point-of-view and should not be highly technical or engineering oriented. The
operation of each maj or component must be addressed without reference to equipment brochures supplied
by subcomponent suppliers.

•	Water Quality Objectives: These shall be of primary importance.

•	Theory of Operation: Text and diagrams shall be provided, as well as a section on

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terminology. These materials shall be at a level understandable by plant operators.

•	Description of the Process: Text and diagrams shall be provided, as well as a section on
terminology.

•	Flow Diagram: Normal operating flow rates and daily cumulative amounts shall be shown.

•	Piping and Instrumentation Diagram: A non-engineering presentation that can be
understood by non-engineer plant operator shall be provided, referencing all valves and components
according to ID labels placed on all components (valves, piping, flow direction, etc.). An ID label and
a clearly marked indicator of OPEN or CLOSED status shall be provided for each valve.

•	Automatic Controller(s): The principle of operation shall be described and the start up
and shut down procedures, reagents required, calibration procedure, accuracy and interface and plant
shall be given.

•	Manual Operation: Method of operation if controllers fail to function shall be described.

•	Data Collection and Recording Devices: A section for each device shall be provided,
describing the operation and accuracy and calibration procedure.

•	Location and Operation of Meters and Instruments: Each item shall be specified and
an ID label given.

•	Alarms.

A section on each of the following shall be included:

•	Start-up procedure

•	Plant settings, service batch, blend percent, brine batch, rinse and backwater water

•	Plant monitoring procedure

•	Expected typical performance

•	Manual shutdown of the plant

•	Restarting the plant after manual shut down alarms

•	Restarting the plant after automatic shut down alarms

•	Alarm removal procedure

•	Reinitializing and restarting

•	Adjusting the plant settings to lower nitrate levels

•	Adjusting the plant to increased nitrate levels

•	Adjusting plant to reduce salt consumption

•	Adjusting plant to reduce waste production

•	Adjusting plant in response to changes in untreated water composition

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16.0 REFERENCES

Anderson, RE., Estimation of Ion Exchange Process Limits by Selectivity Calculations. AIChE.
Symposium Series, 71(152):236, 1975.

Clifford, D A. and Weber, W.J., Nitrate Removal From Water Supplies by Ion Exchange. EPA-
600/2-78-052, June 1978.

Clifford, D. A., et al. Nitrate Removal from Drinking Water in Glendale Arizona, EPA Report PB
87-129 284/AS, 138 pp., 1987.

Guter, G.A. Nitrate Removal From Municipal Water Supplies. Chapter2 in Ion Exchange
Technology, Advances In Pollution Control Edited by Arup Sengupta, Technomic Publishing
Inc,Lancaster, PA, 1995.

Higgins, I.R. Continuous Ion Exchange Equipment, I&EC , 53:336, 1961 EPA-600/S2-82-042,
August, 1992.

Guter, G.A. An Estimation of Effects of Resin and Water Composition on Column Performance in
Nitrate Ion Exchange. A WW A Annual Conference Proceedings, Dallas, Texas, June 10-14,1984 also
see Computer Simulations of Nitrate Removal by Ion Exchange, AWWA Annual Conference
Proceedings, Washington, D.C. June 23 - 27, 1985 p. 1293.

Guter, G.A. Removal of Nitrate from Contaminated Water Supplies for Public Use, Final Report,

EPA-600/S2-82-042, August, 1992.

Guter, G.A.,Hunt, K.,and Paliska, S., Evaluation of Automatic Nitrate Monitoring by Ultraviolet
Absorption at a Nitrate Treatment and Blending Facility, AWWA Water Quality Technology
Conference, Boston Mass., Nov 17-21, 1996.

Lauch, R.P. and Guter, G.A., Ion Exchange for Removal of Nitrate from Well Water, Journal
AWWA (May):83-88, 1986.

Simon, G.P., Ion Exchange Training Manual, Van Nostrand Reinhold, New York, 1991.

Cross-Connection Control Manual, EPA Publication No EPA 570/9-89-007, June 1989.

Recommended Practice for Backflow Prevention and Cross-Connection Control. AWWAManual
M14.

Distribution Valves: Selection, Installation, and Field Testing. AWWAManual M44.

Regulations Relating to Cross-Connections, California Administrative Code, Title 17, Public Health,
1956.

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Table 3. Analytical Methods

Parameters

Laboratory Standard
Methods1 number or Other
Method Reference

Laboratory EPA
Method2

General Water Quality

pH

4500-tf B

150.1 / 150.2

Total alkalinity

2320 B



Temperature

2550 B



Conductivity

2510B

120.1

Total Dissolved Solids

2540 C



Color

2120 B (Hach Company modif. of
SM 2120 measured in
spectrophotometer at 455 nm)



Inorganic Water Qualit

y

Nitrate

4110 B / 4500-NCV D, F

300.0 /353.2

Chloride

4110 B / 4500-C1" D

300.0

Sulfate

4110 B / 4500-SOzf C, D, F

300.0 /375.2

Sodium

3111 B

200.7

Calcium Hardness

3500-Ca+2 D



Total Hardness

2340 C



Alkalinity

2320 B



Iron

3111 D / 3113 B / 3120 B

200.7/200.8/200.9

Manganese

3111 D / 3113 B / 3120 B

200.7/200.8/200.9

Bicarbonate, HC03

Calculation

Organic Water Quality

Total organic carbon

5310 C



Microbiological

Algae, number and
species

10200 F



Heterotrophic Plate Count

9215 B



Notes:

1)	Standard Methods Source: 20th Edition of Standard Methods for the Examination of Water and
Wastewater, 1999, American Water Works Association.

2)	EPA Methods Source: EPA Office of Ground Water and Drinking Water. EPA Methods are available
from the National Technical Information Service (NTIS).

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CHAPTER 4

EPA/NSF ETV
EQUIPMENT VERIFICATION TESTING PLAN
HETEROTROPHIC BIOLOGICAL DENITRIFICATION FOR
REMOVAL OF NITRATE

Prepared By:
NSF International
789 Dixboro Road
Ann Arbor, MI 48105

Copyright 2002 NSF International 40CFR35.6450.

Permission is hereby granted to reproduce all or part of this work, subject to
the limitation that users may not sell all or any part of the work and may not
create any derivative work therefrom. Contact ETV Drinking Water Systems
Center Manager at (800) NSF-MARK with any questions regarding authorized
or unauthorized uses of this work.

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TABLE OF CONTENTS

Page

1.0	INTRODUCTION	4-4

1.1	Application of This Test Plan	4-4

1.2	Objectives of Verification Testing	4-4

1.3	Scope of the PSTP	4-5

2.0 BACKGROUND	4-6

3.0	OVERVIEW OF TASKS	4-7

3.1	Task 1: Characterization of Feed Water	4-7

3.2	Task 2: BD Start-up and Initial Performance	4-7

3.3	Task 3: Product and Residual Management	4-8

3.4	Task 4: Process and Equipment Maintenance	4-8

3.5	Task 5: Data Reduction and Presentation	4-8

3.6	Task 6: Quality Assurance/Quality Control	4-8

4.0 TESTING PERIODS 4-8
5.0 DEFINITIONS	4-9

6.0	TASK 1: CHARACTERIZATION OF FEED WATER	4-10

6.1	Introduction	4-10

6.2	Objectives	4-10

6.3	Work Plan	4-11

6.4	Analytical Schedule	4-12

6.5	Evaluation Criteria	4-14

7.0	TASK 2: BD START-UP AND INITIAL PERFORMANCE	4-14

7.1	Introduction	4-14

7.2	Objectives	4-15

7.3	Work Plan	4-15

7.3.1	Operational Conditions and Start-up	4-15

7.3.2	Response to Transient Loading Conditions	4-17

7.3.3	Response to Extended Periods of Shutdown	4-17

7.3.4	Product Effluent Water Quality	4-18

7.3.5	Chemical Use	4-18

7.3.6	Power Use	4-18

7.3.7	Operator Hours	4-18

7.4	Analytical Schedule	4-19

7.5	Evaluation Criteria	4-19

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TABLE OF CONTENTS (continued)

Page

8.0	TASK 3: PRODUCT AND RESIDUAL MANAGEMENT	4-19

8.1	Introduction	4-19

8.2	Objectives	4-19

8.3	Work Plan	4-20

8.3.1 Sampling Schedule	4-20

8.4	Analytical Schedule	4-21

8.5	Evaluation Criteria	4-22

9.0	TASK 4: PROCESS AND EQUIPMENT MAINTENANCE	4-22

9.1	Introduction	4-22

9.2	Objectives	4-23

9.3	Work Plan	4-23

9.3.1	Process Maintenance	4-23

9.3.2	Equipment Maintenance	4-23

9.3.2.1	Operation	4-24

9.3.2.2	Maintenance	4-24

9.3.2.3	Troubleshooting	4-25

10.0	TASK 5: DATA REDUCTION AND PRESENTATION	4-25

10.1	Introduction	4-25

10.2	Objectives	4-25

10.3	Work Plan	4-25

11.0	TASK 6: QUALITY ASSURANCE/QUALITY CONTROL	4-26

11.1	Introduction	4-26

11.2	Objectives	4-26

11.3	Work Plan	4-26

11.3.1	Daily QA/QC Verifications	4-27

11.3.2	Weekly QA/QC Verifications	4-27

11.3.3	QA/QC Verifications Performed Before Each Test Period	4-27

11.3.4	On-Site Analytical Methods	4-27

11.3.4.1	pH	4-27

11.3.4.2	Turbidity	4-27

11.3.5	Chemical and Biological Samples Shipped Off- Site for Analysis	4-28

12.0 REFERENCES	4-29

TABLES

Table 1 Feed Water Characterization Parameters	4-13

Table 2 Typical Chemical Additives Required for Biological Denitrification	4-18

Table 3 BD System Sampling Schedule	4-21

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1.0 INTRODUCTION

1.1	Application of This Test Plan

This document is the ETV Testing Plan for the Biological Denitrification Process for the Removal of Nitrates
from Contaminated Water. This Testing Plan is to be used as a guide in the development of Product-
Specific Test Plan (PSTP) procedures for testing biological denitrification (BD) treatment equipment, within
the structure provided by the EPA/NSF Environmental Technology Verification (ETV) Protocol Document
for nitrate removal. Refer to the "EPA/NSF ETV Protocol For Equipment Verification Testing For
Removal Of Nitrate: Requirements For All Studies" as well as the Test Plans for Equipment Verification
Testing Plan for Reverse Osmosis and Nanofiltration Processes for further information.

This document is applicable only to heterotrophic carbon-based fixed-film denitrification systems and not to
suspended-growth systems. This document is not applicable to autotrophic denitrification systems including
hydrogen and sulfur-based processes.

Post-denitrification treatment systems for the removal of residual soluble and suspended carbonaceous
materials may be required to bring the biologically denitrified water to drinking water standards. Such
equipment is considered to be a separate treatment module whose performance and operation are outside
the scope of this document. Where such post-treatment is required to reduce the fouling potential of the BD
throughput as measured by turbidity, suspended solids, and residual Total Organic Carbon (TOC), the
reader should consult other NSF publications related to residual contaminant removal including the ETV
document Pro tocolfor Physical Removal of Microbiological and Particulate Contaminants, and Test
Plans for Membrane Filtration, Coagulation and Filtration, Bag and Cartridge Filters, andPrecoat
Filtration. These documents should be useful in determining post-denitrification treatment needs.

In order to participate in the equipment verification process for BD processes, the Equipment Manufacturer
shall retain a Field Testing Organization (FTO) that is NSF-qualified to employ the procedures and methods
described in this test plan and in the referenced ETV Protocol Document as guidelines for the development
of the PSTP. The procedures shall generally follow those Tasks related to Verification Testing that are
outlined herein, with changes and modifications made for adaptations to specific equipment. A
recommended format of the procedures written for each Task should consist of the following sections:

•	Introduction

•	Objectives

•	Work Plan

•	Analytical Schedule

•	Evaluation Criteria

1.2	Obj ectives of Verification Testing

Testing of equipment covered by this Verification Testing Plan shall be conducted by an NSF-qualified
FTO. Water quality analytical work to be carried out as a part of this Verification Testing Plan shall be
contracted with a laboratory that is certified, accredited or approved by a State, a third-party organization
(i.e. NSF), or the U.S. EPA. The FTO shall provide full details of the procedures to be followed for each
task in the PSTP. The FTO shall specify the operational conditions to be evaluated during the Verification
Testing.

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The Manufacturer shall define the verification testing objective(s). These specific objectives of the
equipment verification testing should be different for each Manufacturer, depending upon the statement of
objectives of the specific equipment to be tested. The testing objectives developed by each Manufacturer
shall be defined and described in detail in the PSTP developed for each piece of equipment. The objectives
of the equipment verification testing may include:

•	Verifying the performance of the equipment by generating field data in support of meeting a specific
contaminant level in the treated water;

•	Evaluating new advances in equipment and equipment design;

•	Verifying the performance of the equipment used in a specific environment such as a coastal region
where ocean disposal is available;

•	Verifying the performance of the equipment operating within a specific range of untreated water
quality;

•	Verifying the performance of the equipment used for specific modes of operation such as
continuous or interrupted operation.

Multiple testing objectives may be included in the PSTP. An important aspect in the development of the
verification testing is to describe the procedures that will be used to verify the statement of performance
objectives made for water treatment equipment. A verification testing plan document incorporates the
Quality Assurance/Quality Control (QA/QC) elements needed to provide data of appropriate quality
sufficient to reach a defensible position regarding the equipment performance. Verification testing
conducted at a single site may not represent every environmental situation which may be acceptable for the
equipment tested, but it should provide data of sufficient quality to make a judgment about the application of
the equipment under conditions similar to those encountered in the verification testing.

It is important to note that verification of the equipment does not mean that the equipment is "certified" by
NSF and/or accepted by EPA. Rather, it recognizes that the performance of the equipment has been
determined and verified by these organizations.

1.3 Scope of the PSTP

Specifically, the PSTP shall include at least the following items:

•	Roles and responsibilities of verification testing participants;

•	A brief statement of the objectives of the test plan;

•	A brief statement of the water quality treatment objectives;

•	Procedures governing verification testing activities such as equipment operation and process monitoring;
sample collection, preservation, and analysis; and data collection and interpretation;

•	Experimental design of the Field Operations Procedures;

•	Quality assurance and quality control (QA/QC) procedures for conducting the verification testing and
for assessing the quality of the data generated from the verification testing;

•	Health and safety measures relating to biohazard (if present), chemical, electrical, mechanical and other
safety codes.

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2.0 BACKGROUND

Heterotrophic biological denitrification is a well-established process in the realm of wastewater treatment.
However, this process has not been used on a full-scale basis in the field of water treatment in the U.S., but
there are several full-scale plants being operated in Europe (Dahab et al., 1998; Gayle, et al., 1989). The
primary reason behind the slow transfer of technology from the wastewater treatment to potable water
treatment is the obvious concern over potential contamination of the treated water by bacteria and residual
organics from the bio-denitrification process. This is a legitimate concern that must be kept in mind when
designing such treatment processes for water treatment.

Numerous studies (Dahab and Woodbury, 1998 and Dahab and Kalagiri, 1996) reported on the potential
for using biological denitrification for nitrate reduction in groundwater supplies in laboratory-scale
experiments. The results indicated that fixed-film denitrification can be expected to reduce the nitrate
concentration in the influent water supply from as high as 100 mg/L (as N) to levels within the 1.0 mg/L (as
N) range. These removals translate into an efficiency of nearly 100 percent, which is generally not matched
by other processes available for nitrate reduction. However, some residual soluble as well as insoluble
organic matter should be expected in the denitrified water supply. Further treatment can reduce these solids
to levels sufficient to meet prevailing drinking water standards.

In heterotrophic biological denitrification, facultative microorganisms are contacted with the water supply
containing nitrates and an added carbon source in an anoxic (oxygen-free) environment. Under these
conditions, the bacteria utilize nitrates as a terminal electron acceptor in lieu of molecular oxygen. In the
process, nitrates are reduced to nitrogen gas, which is harmless and can be directly discharged to the
atmosphere. The extraneous carbon source is necessary since it supplies the energy required by the
microorganisms for respiration and synthesis while serving as an electron donor. Most denitrification studies
have used methanol (CH3OH) as the carbon source. If a simple carbon source is chosen such as ethanol or
acetic acid, then the biomass produced during the process should be correspondingly low; a useful
characteristic in that the overall excess biomass production is minimized.

Since heterotrophic denitrifying bacteria require an organic carbon source for their respiration and growth, a
wide variety of organic compounds have been used. These organics include methanol, ethanol, acetic acid,
glucose, and other more complex organics. While the types of organic compounds may affect the biomass
yield, the choice is generally based on economic comparison. The availability of ethyl alcohol from
agricultural sources could make this carbon source a strong candidate for denitrification systems. It should
be noted that methanol toxicity is such that it is not recommended as electron donor and carbon source for
drinking water denitrification.

Another important factor is the presence of dissolved oxygen in the waters and its inhibiting effects. To
effect denitrification, the oxygen concentration must be reduced to a level low enough to avoid inhibition or
repression of nitrate reductase. Unless dissolved oxygen is removed by chemical addition, the amount of
electron donor (organic carbon) added must be equal to that needed to remove the oxygen as well as the
nitrate.

Biological denitrification can be carried out in suspended or attached growth systems. In suspended growth
systems, the bacterial culture is "suspended" within the contents of the reactor vessel by constant mixing or
agitation. In these systems, sedimentation is required to settle out the bacterial biomass so it can be returned
to the reactor vessel, or otherwise removed by wasting. Such systems are common in wastewater treatment

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applications. The principal advantages of suspended growth systems include the ability constantly return
biomass into the system and small tankage requirements. However, suspended growth systems are subject
to damage or washout by hydraulic transients and influent shock loads. They are generally not suited for
handling periods of extended shutdown.

In fixed-film (also known as biofilm) systems, the bacterial biomass is physically attached to a solid matrix,
which serves to support the bacterial mass by providing surface area on which the bacteria can grow in a
film-like layer. Attached growth systems can be of the static media type or the expanded-bed (i.e. fluidized)
type. In static media systems, the solid matrix typically is made up of synthetic modules that are stacked in
some fashion (or simply dumped, depending on their size and configuration) in the reactor vessel. These
media can have high porosity, light weight (when synthetic materials are used) and high specific surface area
(i.e. surface area per unit volume of medium). Static media attached growth systems are operated in either
downflow or upflow regimes although upflow systems are more common due to the reduced chance of
plugging associated with their operation and the fact that the bacterial biomass is constantly submerged.

Fluidized-bed systems are operated in an upflow manner so that the bacterial growth matrix bed is
expanded hydraulically as the water is pumped from the bottom to the top of the reactor. In expanded-bed
systems, the support media are generally of the granular type (both natural and synthetic) to facilitate
expansion of the bed. As the bed is expanded the entire surface of the granular material is made available
for bacterial support. Because of this fact, expanded-bed systems have been reported to be loaded at rates
exceeding static-bed systems. However, the additional costs associated with pumping to maintain bed
expansion or fluidization must also be considered during design evaluation. With no known exceptions, all
full- scale biological denitrifi cation systems designed for potable water treatment have been of the static-bed
fixed film type.

3.0	OVERVIEW OF TASKS

This ETV Testing Plan is divided into 6 tasks. A brief overview of the tasks to be included in the
verification testing program is presented below:

3.1	Task 1: Characterization of Feed Water

A full characterization of the source water must b e made prior to initiating operation so that the potential for
fouling and mineral precipitation (scaling) and other possible interferences can be defined and/or predicted.
Results of this analysis shall be used to define feed water pretreatment requirements, chemical doses and
system operating conditions, and to identify potential foulants in the source water for monitoring during
operation.

3.2	Task 2: BD Start-up and Initial Performance

The objective of this task is to evaluate BD start-up and subsequent steady-state operation. Start-up
conditions including the need for bacterial seed (inoculum) must be characterized. Furthermore, the usability
of the biological denitrification process and potential fouling (including excess biomass and potential
hydrogen sulfide production) shall be evaluated in relation to feed water quality.

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3.3 Task 3: Product and Residual Management

The objective of this task is to evaluate the quality of water produced by the BD system, referred to as
product water or BD effluent. Multiple water quality parameters shall be monitored during each operational
period. A basic goal of this Task is to confirm that BD-treated waters meet the manufacturer's
performance objectives for nitrite. BD effluent quality shall be evaluated in relation to feed water quality and
operational conditions to determine if additional treatment is required. Any wastewater or sludge streams
shall also be characterized and management plans for the proper disposal of BD residual biosolids are to be
identified.

3.4	Task 4: Process and Equipment Maintenance

An important aspect of BD operation is the maintenance of the system so that an adequate inventory of
biological (bacterial) mass (i.e. biomass) is maintained while avoiding excess biomass conditions that may
lead to impaired effluent quality or to system support matrix clogging. Another intent of this task is to
provide procedures and methods for insuring the continued integrity and operability of all equipment and
associated appurtenances.

3.5	Task 5: Data Reduction and Presentation

The objective of this task is to establish an effective field protocol for data management at the field
operations site and for data transmission between the FTO and NSF for data obtained during the
Verification Testing.

3.6	Task 6: Quality Assurance/Quality Control

An important aspect of Verification Testing is the protocol developed for quality assurance and quality
control. The objective of this task is to assure accurate measurement of operational and water quality
parameters during Verification Testing.

4.0 TESTING PERIODS

On the assumption that the source water is a groundwater, which normally exhibits minor or no significant
changes in seasonal water quality, the required operational tasks in the Verification Testing Plan (Tasks 1 -4)
shall be performed at least once during a 1-year period, not including mobilization, start-up, and Initial
Operations.

A minimum of one verification testing period shall be performed. Additional verification testing periods may
be necessary if feed water quality is known to be seasonally variable. If one verification testing period is
selected, the feed water should represent the worst-case concentrations of nitrate concentration, which can
verify the manufacturer's objectives. Although one testing period satisfies the minimum requirement of the
ETV program, manufacturers are encouraged to use additional testing periods to cover a wider range of
water quality conditions. Verification testing periods consist of continued evaluation of the treatment system
using the pertinent treatment parameters defined through feed water characterization. Verification test
periods shall last a minimum of3000-hours. The purposes of the 3000-hour test period are: 1) to provide a
data base for a long period of time to demonstrate start-up (establishment of biomass in system) and

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steady-state conditions; 2) to assess the system recovery after short periods of shutdown; and 3) to provide
opportunity for the treatment of feed water having variable quality.

5.0	DEFINITIONS

5.1	Biological Denitrification: a bacteria-mediated (i.e. biological) process in which nitrate is
reduced into nitrogen gas by denitrifying bacteria (typically facultative heterotrophes) under anoxic (oxygen-
free) conditions. The process requires that an electron donor (typically an organic carbon source) be
present for the reaction to go to completion.

5.2	BD Effluent: Product water produced by the biological denitrification (BD) treatment system.

5.3	Denitrified Effluent: The same as BD Effluent.

5.4	System Feed Water: Source water introduced into the BD process for treatment.

5.5	Reactor Feed Water: Influent feed water introduced into the BD reactor system, consisting of
raw system feed water, and in rare occasions, a combination of raw system feed water and recycled reactor
effluent being returned for further treatment.

5.6	Fouling: A condition in which the BD effluent quality is impaired by the presence of excess
biological solids being discharged by the BD reactor or fouling resulting from extraneous reactions including
sulfate reduction to produce traces of hydrogen sulfide. Fouling in this case also is referred to as
"biofouling". Fouling could also be the result of other contaminants that might be present in the feed water.

5.7	Carbon Source: The material to be used as a source of elemental carbon for heterotrophic
bacterial denitrification to proceed. The organic carbon serves as an electron donor in the heterotrophic
biological denitrification reaction as well as a source of energy for bacterial metabolism. This carbon source
can be one (or a combination of) several organic substances including alcohols (such as methyl alcohol or
ethyl alcohol), organic acids (such as acetic acid) or other similar organic substances including sugars.

5.8	Electron Donor/Electron Acceptor: The term electron donor generally refers to the organic
carbon source to be used in the heterotrophic denitrification process whereas the electron acceptor refers to
substances being reduced in the denitrification process, principally nitrate, but it also may include sulfate, if
present in the raw feed water being denitrified.

5.9	Bacterial Seed (Inoculum): This term refers to the bacteria that must be added to the
denitrification reactor to accomplish the conversion of nitrate biologically into nitrogen gas. Such bacteria
must be inoculated into the reactor system for immobilization on the reactor bacterial support matrix.
Bacterial inoculums can be of a pure culture or a mixed culture variety.

5.10	Reactor: The term reactor refers to the vessel in which the denitrification process is to be
accomplished. The combination of the reactor vessel, bacterial support matrix and other related
appurtenances are referred to as the "Reactor System."

5.11	Start up: The period of time following the installation of the reactor system, addition of bacterial
seed and the commencing of operation. During this period, the product water may contain excess biomass

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and excessive nitrate concentrations indicating that the treatment process has not stabilized yet. Generally,
start-up should be in the order of 3 to 5 weeks.

5.12	Steady-State Operation: Steady-State Operation begins after the reactor system had gone
through a successful start-up with nitrate concentrations in the reactor effluent approaching the targeted
concentrations and with minimal occurrence of fouling.

5.13	Biofllm: Biofilm is the structural appearance of bacterial mass (biomass) on the surface of the
reactor support matrix. Ideally, the biofilm should be a consistent and uniform accumulation of bacterial
solids that appear like a gelatinous and slime-like layer that can be put into contact with the water being
treated for the removal of nitrate contamination.

5.14	Support Matrix: The porous material to be used as reactor column packing to support the growth
and accumulation of denitrifying bacteria. These materials typically consist of small individual packing
modules that can be randomly packed into the reactor or large modular blocks that can be stacked inside
the reactor column. These matrices must be made of materials that are known to be non-reactive and non-
leachable and non-biodegradable and be lightweight with high specific surface area and high porosity.

5.15	Specific Surface Area: The amount of surface area (e.g. square feet) provided by a unit volume
(e.g. one cubic foot) of packing material.

5.16	Porosity: The extent of open space provided by the biological denitrification reactor packing
material; generally computed as the volume of voids per unit volume of packing material.

5.17	Post-denitrification Treatment: Refers to all treatment methods that are required to bring
biologically-denitrified water to drinking water quality and likely to include filtration and disinfection.

6.0	TASK 1: CHARACTERIZATION OF FEED WATER

6.1	Introduction

This task involves a complete characterization of the raw water being fed to the treatment system. The
information is required to determine the suitability of the water source as a feed water for verification testing,
and to document parameters which may be important in predicting the treatability of the water source and
treatment efficiency of the treatment process.

6.2	Objectives

The objectives of this task are as follows:

•	Obtain a complete physical, chemical, and biological characterization of the source water or
feed water that will be treated.

•	Determine the degree of nitrate removal needed and the amounts of the organic carbon source
and other chemicals required to carry out the denitrification process.

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•	Identify potential process contaminants (foulants) such as sulfates, bacterial solids, etc., that
might affect the treatment process, and to determine potential and degree of feed water
pretreatment, if any, that may be needed during system operation.

•	Verify that the water, as sampled, is representative of the source water based on historical data
(where available).

6.3 Work Plan

This Verification Testing Plan is based on the assumption that biological nitrate removal will be
predominately applied to groundwater which is not subj ect to significant seasonal changes in water quality or
temperature. Water sources with significant variability in nitrate contamination, including surface waters,
require a significantly different approach to address seasonal variations in water quality. With the exception
of "effluent" streams that receive a significant component of their base flow from nitrate-contaminated
groundwater sources, surface water supplies typically have not historically exhibited nitrate contamination
that would require treatment.

In cases where the feed water quality is known to vary seasonally, sufficient information shall be obtained to
illustrate the variations that are expected to occur in the parameters that will be measured during Verification
Testing for a period of time long enough to demonstrate such variability. This information shall be compiled
and provided to NSF, so NSF and the FTO can determine the adequacy of the data for use as the basis to
make decisions on the testing schedule. The initial characterization is important to the success of the testing
programs, as failure to adequately characterize the feed water (source water) could result in testing at a later
site deemed inappropriate. Therefore, the initial characterization is important to the success of the testing
program.

A brief description of the aquifer system that provides the feed water shall be provided in the PSTP to aid in
interpretation of feed water characterization results. In addition to water quality parameters, this description
should include aquifer hydrogeologic characteristics that may influence the groundwater quality.
Furthermore, a brief description of watershed(s) hydrologic characteristics that may have an influence on the
aquifer water quality should also be provided. The watershed description should include a statement of the
approximate size of the watershed, a description of its soils (i.e. clays, silts, sand, etc.) and their
hydrogeologic characteristics as well as a description of it topography (i.e. flat, gently rolling, hilly,
mountainous, etc.). A description of the kinds of human activities that take place (i.e. mining, manufacturing,
cities or towns, farming) with special attention to potential sources of pollution that might influence feed
water quality should also be provided. The nature of the water source, such as stream, river, lake, or man-
made reservoir, should be described as well.

Most water sources will not have pre-existing water quality data of sufficient detail to allow an evaluation of
the proper application of biological denitrification. Completion of this task involves the following:

•	Analysis of grab samples for a detailed water quality analysis. The parameters evaluated shall
allow for the calculation of a complete cation/anion balance, in addition to general physical,
chemical and biological measurements and limited organic analysis.

•	A review of selected historical water quality data, where available. This should allow for the
determination of trends in key water quality parameters such as nitrate, sulfate, alkalinity and

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total dissolved solids (IDS) (or conductivity), as well as allowing for the verification that the
water quality measured by the grab samples is representative of recent historical data.

•	Calculation of the amounts of nitrates to be removed to meet existing drinking water quality
standards as well as the amounts of organic carbon needed to satisfy the stoichiometric needs
of denitrifying microorganism.

•	Calculation of the buffering capacity of the feed water and the need to control pH. Assessment
of the viability of the biological denitrification process as well as other potential interferences
that may lead to biofouling. This includes estimating the concentrations of the following salts
and species in the feed water stream:

•	carbonate/bicarbonate,

•	total alkalinity,

•	sulfate,

•	phosphates, and

•	iron and manganese.

The FTO shall include in the PSTP guidelines for maximum concentrations for each of the above salts during
BD system operation, assuming the use of appropriate water pretreatment and conditioning chemicals, when
needed.

6.4 Analytical Schedule

Parameters required for a complete evaluation of source water quality are presented in Table 1. This table
identifies all required parameters for evaluation in the field or in the laboratory by a laboratory that is
certified, accredited or approved by a State, a third-party organization (i.e. NSF), or the U.S. EPA. In
order to ensure that the source water supply is of consistent quality, it is recommended that the feed water
be re-evaluated periodically (monthly basis is recommended) according to Table 1 during ETV testing.
Parameters in Table 1 that are found to be constant after repeated testing over time can be removed from
periodic testing. Table 1 also identifies the recommended Standard Methods (APHA, 1992) or U.S. EPA-
approved procedures.

Parameters to be analyzed from grab samples shall be taken in duplicates, as a minimum, or more, at least
one week apart. Potential sources of historical data include the United States Geological Survey (USGS),
US Environmental Protection Agency, and state and local approved laboratories.

Manufacturers and suppliers intending to have their equipment verified for uses other than biological nitrate
removal may wish to characterize the source water in terms of additional parameters besides those identified
in Table 1.

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Table 1. Feed Water Characterization Parameters

Parameter

Analysis Options

Standard Methods1
number or Other Method
Reference

EPA Method2

Field

On-Line

Lab

General Water Quality

PH

X

X



4500-pr B

150.1 /150.2

Total alkalinity





X

2320 B



Total Hardness





X

2340 C



Calcium Hardness





X

3500-Ca D



Temperature

X

X



2550 B



Conductivity

X





2510

120.1

Total Dissolved Solids





X

2540 C



Total Suspended Solids





X

2540 D



Turbidity

X

X



2130 B/Method 2

180.1

Color





X

2120 B (Hach Company modif.
of SM 2120 measured in
spectrophotometer at 455 nm)



Taste and Odor3





X

2150-2160



Inorganic Water Quality

Sodium





X

3111 B

200.7

Potassium





X



200.7

Ammonia, NH4





X



350.3

Strontium





X



200.7

Barium





X

3111 D/3113 B/3120B

200.7/200.8

Iron





X

3111 D/3113 B/3120B

200.7/200.8/200.9

Oxygen, Dissolved

X





4500-0



Manganese





X

3111 D/3113 B/3120B

200.7/200.8/200.9

Carbonate, C03





X

Calculation

Bicarbonate, HC03





X

Calculation

Sulfate, S04

X



X

4110 B / 4500-S04= C, D, F

300.0/375.2

Chloride

X



X

4110 B / 4500-C1" D

300.0

Nitrate, N03

X



X

4110 B / 4500-N03" D, F

300.0/353.2

Nitrite, N02

X



X

4110 B / 4500-N03" D, F

300.0/353.2

Fluoride





X

4110B/ 4500-F" B, C, D, E

300.0

Carbon Dioxide, C02





X

6211 M



Hydrogen Sulfide, H2S

X







376.1/2

Organic Water Quality

Total organic carbon





X

5310 C



Electron Donor4

X



X

TBD



UV254 absorbance





X

5910 B



AOC/BDOC





X

9217A/5310



Microbiological

Total coliform





X

9221, 9222, 9223



Heterotrophic Plate Count





X

9215 B



1)	Standard Methods Source: APHA (1999).

2)	EPA Methods Source: EPA Office of Ground Water and Drinking Water (1996).

3)	Taste and Odor measurements are optional.

4)	Residual electron donor (i.e. carbon source) concentration; TBD = To be determined by the FTO. See Section
5.7, FTO must identify Standard Method for their carbon source of interest (i.e. alcohols, organic acids, or sugars).
Note that the choice of electron donor could interfere with ion chromatography-based measurements.

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6.5 Evaluation Criteria

Feed water quality shall be evaluated in the context of the Manufacturer's statement of performance
objectives. The feed water should challenge the capabilities of the equipment with respect to nitrate
concentration, but should not be beyond the range of water quality parameters suitable for treatment for the
equipment in question.

The detailed chemical analysis should lead to a detailed determination of chemical doses required to support
and maintain the biological process including organic carbon, pH control, dissolved oxygen control, and
other process maintenance measures. Furthermore, the detailed water quality analysis results should allow
for the determination of feed water constituents that may cause interferences, or potential biofouling, during
biological denitrification. The analysis should lead to the proper selection of the chemical pretreatment
options (i.e. chemical addition), if needed, to control and minimize such interferences.

If the feed water does not contain the level of nitrate concentration required to verily the manufacturer's
removal objectives, nitrate spiking may be employed. The nitrate spiking procedure must be a peer-
reviewed and published procedure and it must be reviewed by NSF and the EPA priorto implementation.
Manufacturers and FTOs should also be aware that there are professional opinions that are opposed to
nitrate spiking for verification testing.

7.0	TASK 2: BD START-UP AND INITIAL PERFORMANCE

7.1	Introduction

The purpose of this task is to verily that the BD system, when tested in accordance with Manufacturer-
selected operating conditions using the selected source water, can reach and maintain performance as
defined by:

•	Productivity (product flow)

•	Nitrate concentration (and other salts, if applicable)

•	Concentrations of residual dissolved and suspended organic solids in the BD effluent

•	Degree of post- denitrification treatment required to elevate the water quality to drinking water
standards.

Another purpose of this task is to demonstrate that changes in the level of these performance characteristics
caused by biofouling or other interactions between the BD system and the feed water can be adequately
managed through conventional process modifications, chemical addition, or through conventional post-
denitrification treatment.

The start-up of biological treatment systems requires the selection and enrichment of a non-pathogenic
biological culture capable of efficiently and selectively removing nitrate from water. This biological culture
must be accumulated within the reactor packing material to provide constant and steady-state nitrate
removal. The accumulation of such solids will require careful operation during the start-up period to avoid
the sudden washout of biological solids due to hydraulic transient conditions. Furthermore, the biological

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system will require careful monitoring to assure that minimum trace elements and buffer are provided and
that excess nitrate loading is avoided. Additionally, suitable environmental conditions including moderate
temperature and pH levels must be maintained. Although biological denitrification can be carried out at all
normal temperatures above freezing, the maintenance of moderate temperature (i.e. 50- 80 F) is conducive
to desirable denitrification rates.

Beyond start-up, the BD system must be operated under steady-state operating conditions as specified by
the FTO. While product flow is maintained, measures shall be taken to optimize (i.e. reduce) the
concentrations of residual soluble and suspended organic materials (including bacterial numbers) in the
denitrified effluent to minimize post-denitrification treatment needs.

As the biomass inventory in the system increases to the point where it is measurably impacting the BD
system effluent quality, this biomass inventory will need to be managed as described in Task 4 to reduce the
concentrations of residual suspended organic matter in the system effluent.

In the event that biofouling is judged to be excessive and unacceptable, the Manufacturer shall propose
revised operating conditions to reduce such fouling. The effect of corrective measures on water productivity
and BD effluent quality water shall then be determined through additional testing.

Prior to the start of the Verification Testing Program, the operational conditions to be verified shall be
specified by the FTO in terms of an average water production rate (gallons per day [gpd]), nitrate removal
efficiency, and organic carbon use rates.

7.2	Objectives

The objectives of this task are to document the following:

•	Conditions for the BD system start-up and long-term operation.

•	Performance of the BD system when operated under variable loading conditions.

•	Effluent quality achieved by the BD system.

•	Chemicals use and their impact on the treated water supply

7.3	Work Plan

7.3.1 Operational Conditions and Start-up

The PSTP shall specify information concerning the design and operation of the BD treatment system
being evaluated in the following categories: 1) system design criteria; 2) operating conditions; 3)
written procedures for operation and maintenance; and 4) maintenance criteria. To achieve and
maintain successful long-term biological denitrification, numerous considerations need to be kept in
mind during the various phases of reactor selection, design, start-up and operation. These
considerations are discussed briefly below:

1. Reactor selection and configuration: To provide an acceptable level of system
reliability, care must be taken during the BD system reactor selection and configuration. For
example, the use of single-stage systems can be acceptable provided that more than one
reactor is provided to meet minimum reliability requirements. When economically justifiable,

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two-stage systems can provide a crucial degree of reliability to ensure long-term operation and
maintenance. This level of reliability can yet be increased by utilizing multiple trains of two stage
systems. Work by numerous researchers has demonstrated that two-stage reversible-flow
reactor configurations are extremely efficient in providing increased levels of operational
reliability beyond that provided by once-through two-stage systems (Siddique, and Young,
1995; Dahab andKalagiri, 1996; Dahab and Woodbury, 1998). ThePSTP should justify the
use of single-stage systems, or single-stage systems with recycle over two-stage systems based
on process as well as economic considerations.

Furthermore, the type of packing media should be specified based on expected hydraulic flow
regime, packing media porosity, specific surface area and other pertinent considerations
including materials of construction of packing media, density and chemical characteristics.
Packing materials that are known to be non-reactive, non-leachable and non-biodegradable
should be selected.

2.	Operational Controls: The PSTP shall delineate the BD process and its controls based
on kinetic as well as process hydraulic considerations, reactor dimensions, flow rates, influent
nitrate concentrations, and proposed detention times. The PSTP shall also delineate steps to
optimize theBD system by consideration of split (partial) treatment to provide for sufficient, yet
flexible, denitrification capacity to meet the nitrate and nitrite standards while minimizing the total
flow requiring actual biological treatment (i.e. treating a portion of the flow and blending it with
the remaining untreated portion).

3.	Reactor Seeding and Seed Selection: The PSTP shall specify how the BD system is to
be seeded with denitrifying culture and how such non-pathogenic culture is to be obtained.
There are many species of non-pathogenic bacteria that are capable of denitrification which are
found in soils and natural waters. Such bacteria can be harvested and enriched for inoculation
of the BD reactors. Many such bacterial cultures also can be obtained from commercial
suppliers.

4.	Carbon Source Selection: The most efficient species of denitrifying bacteria are typically
facultative heterotrophs that require organic carbonaceous material be added as a source of
energy for growth and multiplication. The PSTP shall specify the type of carbon to be provided
for metering into the feed water. Typically, simple organic carbon sources are the most efficient
from the standpoint of minimizing the amount of biosolids production as well as being fully
utilized during the biodenitrification reaction. Additionally, the organic carbon source selection
is typically based on cost as well as public health considerations. For example, while being a
fully biodegradable simple organic molecule, methyl alcohol may not be acceptable because of
potential toxicity implications. Based on economic and kinetic considerations, ethyl alcohol is
an ideal denitrification carbon source, but it may, or may not be desirable from the standpoint of
public acceptance.

5.	Dissolved Oxygen (DO) Control: Dissolved Oxygen (DO) control is important to
maintain anoxic conditions for optimum denitrification in the reactor system. The PSTP shall
specify the methods by which dissolved oxygen would be reduced, if present in significant
concentrations. DO can be removed by the addition of a reducing agent (e.g. sodium sulfite) or
by relying on aerobic carbonaceous bacterial reaction to consume the DO in the feed water.

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The latter method implies that additional organic carbon would need to be provided to satisfy
the dissolved oxygen demand. Subsequent to biological denitrification, it may be desirable to
restore the DO concentration in the treated water. This measure generally should contribute to
the improvement of the chemical and aesthetic quality of the treated water as DO will be helpful
in the oxidation and removal of residual dissolved and suspended organics in the treated water
as well as help reduce potential malodorous conditions. The PSTP should address this
important issue by examining the need for post-denitrification oxygen addition.

6.	Product Gas Removal: The PSTP shall indicate how gases produced during
denitrification will be exhausted from the reactor system. These gases are typically made of
nitrogen gas and carbon dioxide and thus, need to be properly vented to the atmosphere.

7.	Control of pH: The PSTP shall specify methods to monitor and control pH levels in the
treated water supply, if necessary. Typically, biological denitrification will result in increasing
the water pH. Depending on the influent nitrate concentration in the feed water and the
available buffering capacity of the water, pH control might be necessary. Fortunately, most
groundwater supplies will contain sufficient alkalinity to counteract this phenomenon, assuming
that nitrate contamination is moderate.

8.	Excess Biomass Production: During biological denitrification, a certain amount of
biomass is produced and accumulated in the reactor as either attached biofilm or suspended
biomass floe, biomass granules, or similar agglomerations. The PSTP shall specify methods of
biosolids control including wasting frequency and reactor backwash procedures, if necessary.

7.3.2	Response to Transient Loading Conditions

The ability of the BD System to respond to changes in loading conditions shall be determined after
steady state operation is reached and maintained for a period of about 3 -4 weeks. Measures shall
be taken to challenge the system's ability to respond to transient increases in nitrate concentration
and/or hydraulic loading rates. This can be accomplished by altering the loading rates to the BD
system for a duration equal to at least 3-4 hydraulic detention times. If the nitrate concentration in
the water supply is not sufficiently variable, then this can be accomplished by gradually increasing
the hydraulic loading rate by at least 50 percent (ideally in increments spanning about 1-2 hydraulic
detention times). The BD reactor system performance shall then be monitored throughout the
transient test period. If the water supply nitrate concentration is known to be variable, then the
system can be operated at a constant flow rate while observing and documenting the reactor
performance for a period of time of sufficient length such that a 40-50% change in nitrate
concentration, if possible, is observed.

7.3.3	Response to Extended Periods of Shutdown

The ability of the BD System to respond to periods of dormancy, or shutdown, shall be determined
after steady state operation is reached and maintained for a period of about 3-4 weeks. The ability
of the system to respond to extended shutdown can be accomplished by gradually turning the flow
to the system off, keeping it off for a period of 5-6 days, and then gradually restarting the system.
The BD reactor system performance shall then be monitored throughout the restart-up period and
until normal performance is re-established.

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7.3.4 Product Effluent Water Quality

The key parameters in measuring the quality of the biologically treated water are the effluent nitrate
concentration and the concentration of other substances, organic and inorganic, that can result from
the biological treatment process. These byproducts are generally considered to be foulants and
thus must be reduced to acceptable levels. The PSTP shall address this issue by providing detailed
product water analysis and specifying steps to reduce, or remove foulants resulting from water
treatment. These issues are further detailed in Task 3.

7.3.5 Chemical Use

Successful biological denitrification requires the use of chemicals that can facilitate the vitality of the
biological culture that removes nitrate from water as well as re-condition the treated water quality to
meet prevailing drinking water quality. Typical chemical additives are listed in Table 2 below. The
PSTP must address the need for chemical addition either as pre-denitrification or as post-
denitrification additives and specify chemical addition rates, metering and dosing systems and
provide for proper storage and handling facilities for these substances.

Table 2. Typical Chemical Additives Required for Biological Denitrification.

Chemical

Purpose

Use/Addition

Organic Carbon sources including Ethanol,
Acetic Acid, Acetate, and Others

Organic Carbon
Source

Pre- denitrification

Hydrochloric acid

pH control

Denitrification

Bicarbonate

pH control

Denitrification

Phosphate

P-source

Pre- denitrification

Phosphoric Acid

P-Source

Pre- denitrification

Sulfite, Sodium

DO Control

Pre- denitrification

Oxygen

DO Control, Oxidant

Post- denitrification

7.3.6	Power Use

In an attempt to calculate power costs for operation of the system, power usage shall be measured
by meter readings or quantified by the following measurements: pumping requirements, size of
pumps, nameplate voltage, current draw, power factor.

7.3.7	Operator Hours

In an attempt to calculate labor hour costs for operation of the system, operator hours shall be
recorded during the verification testing.

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7.4	Analytical Schedule

During Verification Testing of the BD system equipment, the feed water and treated water quality shall be
characterized by measurement of the "Field" water quality parameters listed previously in Table 1. These
data are to be collected and analyzed to enhance the usefulness of the Verification Testing data.

The sampling schedule and sampling frequency shall conform to sampling schedule and sampling frequency
defined in Task 3 (Product and Residuals Management) and Table 3.

7.5	Evaluation Criteria

Where applicable, the data developed from this task should be compared to statements of equipment
performance objectives. If no relevant statement of performance capability exists, results of operating and
performance data should be tabulated for inclusion in the verification report.

8.0	TASK 3: PRODUCT AND RESIDUALS MANAGEMENT

8.1	Introduction

Under normal conditions, BD involves the production of minor amounts of wastewater; most of which is
associated with the flushing and periodic backwash of the denitrification reactors. This task involves a
characterization of product and waste water quality during the system operation described in Task 2.
Product water analysis shall serve to document that the treatment system meets the nitrate removal
performance criteria for which the manufacturer is seeking verification. Additional water quality information
is required to identify performance of the treatment system relative to any potential fouling identified during
the raw water characterization performed in Task 1.

The quality and quantity of wastewater produced by the BD treatment system is an important consideration
in determining the appropriate methods of management and disposal of this wastewater. The cost of
wastewater disposal typically is not a large component of the total system cost, but it can be significant
depending on the type of disposal option selected, particularly for those not utilizing a direct discharge to an
existing wastewater treatment system.

8.2	Objectives

The objectives of this task are as follows:

•	Assess the ability of the biological treatment system equipment to meet the water quality goals
specified by the Manufacturer.

•	Assess the amount and concentrations of any potential foulants which may interfere with the
long-term operation of the treatment system. Examples include excessive sulfate concentrations
that can lead to the production of sulfide and other dissolved and suspended solids that can
interfere with the biological treatment process.

•	Characterize the volume and concentration of the wastewater produced by the process.

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8.3 Work Plan

Water quality data shall be collected for the BD treatment system feed water and product during the BD
test as outlined in Task 2. As a minimum, the required sampling schedule identified in Table 3 shall be
observed by the FTO on behalf of the Manufacturer. Water quality goals and target removal goals for the
BD equipment shall be clearly delineated in the PSTP.

When necessary, excess solids must be removed from the reactor system to maintain a suitable and
adequate solids inventory in the reactors. When solids removal is implemented, characterization of the
amount and quality of wastewater that needs to be removed must be completed. The biological solids
(biosolids) to be removed from these reactors are non-toxic, but they must be handled and managed in an
approved manner in consultation with the requisite regulatory agencies. The most appropriate method of
disposal might be the discharge of these solids and wastewater to a nearby municipal biological wastewater
treatment system with adequate capacity to handle the solids load. Otherwise, solids concentration (i.e.
thickening and/or dewatering) might be needed before disposal of these solids to land as a soil conditioner.
Additional disposal methods might be available. The PSTP must specify, based on local conditions and
regulations, the method(s) of solids handling and ultimate disposal.

8.3.1 Sampling Schedule:

The sampling schedule outlined in Table 3 applies to the biological denitrification system raw feed
water and treated water streams. Some parameters identified in Table 3, including nitrate and
nitrite, should be analyzed on a continuous basis, or on a multiple daily measurement basis, as
appropriate. However, when continuous analysis is not possible, these parameters shall be
analyzed at least once every four to six hours.

The FTO shall identify the treated water quality objectives to be achieved in the statement of
performance objectives of the equipment to be evaluated in the Verification Testing Program. The
statement of performance objectives prepared by the FTO shall indicate the range of water quality
under which the equipment can be challenged while successfully treating the feed water.

Many of the water quality parameters described in this task shall be measured on-site by the FTO
or by a laboratory that is certified, accredited or approved by a State, a third-party organization
(i.e. NSF), or the U.S. EPA.

The methods to be used for measurement of water quality parameters in the field are summarized in
Table 3. The analytical methods utilized in this study for on-site monitoring of influent and product
water qualities are described in Standard Methods (APHA, 1992) and/or the U. S. EPA. Methods
and are governed by their respective QA/QC measures. Where appropriate, the Standard
Methods reference numbers and EPA method numbers for water quality parameters are provided
for both the field and laboratory analytical procedures.

For the water quality parameters requiring analysis by a laboratory that is certified, accredited or
approved by a State, a third-party organization (i.e. NSF), or the U.S. EPA, water samples shall
be collected in appropriate containers (using recommended sample preservatives techniques, as
applicable) prepared, or otherwise approved, by the laboratory. These samples shall be preserved,
stored, shipped and analyzed in accordance with recommended procedures and holding times, as
specified by the analytical lab.

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TABLE 3. BD SYSTEM SAMPLING SCHEDULE DURING ETV TESTING

Parameter

Sampling
Frequency1

Analysis
Options

Standard Methods2
number or Other Method
Reference

EPA Method3

Nitrate, NO3

Multiple Daily (or
continuous)

Field or Lab

4110 B / 4500-N03" D, F

300.0 / 353.2

Nitrite, NO2

Multiple Daily (or
continuous)

Field or Lab

4110 B / 4500-N03" D, F

300.0 / 353.2

pH

Multiple Daily (or
continuous)

Field or On-
line

4500-tf B

150.1 / 150.2

Temperature

Daily

Field or On-
line

2550 B



Dissolved Oxygen

Multiple Daily (or
continuous)

Field

4500-0



Turbidity

Multiple Daily (or
continuous)

Field or On-
line

2130 B Method 2

180.1

TDS

Weekly

Lab

2540 C



Conductivity

Weekly

Field

2510



Sulfate, S04

Daily

Field or Lab

4110 B / 4500-S04= C, D, F

300.0 /375.2

Sulfide

Daily

Lab

4500-S= F, D



TSS

Daily

Lab

2540 D



VSS

Daily

Lab

2540 E



TOC

Daily

Lab

5310 C



DOC

Daily

Lab

5310 C



Carbon source
(electron donor)

Daily

Field or Lab

TBD



Total Coliform

Weekly

Lab

9215 B/9221 / 9222/9223



E. Coli

Weekly

Lab

9221 / 9222 / 9223 (Colilert)



UV Absorbance (254
nm)

Daily

Lab

5910 B



Alkalinity

Daily

Lab

2320 B



Total Hardness

Weekly

Lab

2340 C



Color

Weekly

Lab

2120 B (Hach Company
modif. of SM 2120 measured
in spectrophotometer at 455
nm)



Taste and odor4

Weekly

Lab

2150-2160



Iron and Manganese

Weekly

Lab

3111 D/3113 B/3120B

200.7/200.8 /
200.9

Chloride

Weekly

Field or Lab

4110 B / 4500-C1" D

300.0

1	Grab samples unless continuous sampling.

2	Standard Methods Source: APHA (1999).

3EPA Methods Source: EPA Office of Ground Water and Drinking Water (1996).

4Taste and Odor measurements are optional.

8.4 Analytical Schedule

The minimum sampling frequency for the required Task 3 water quality parameters is presented in Table 3.
At the discretion of the FTO, the water quality sampling program may be expanded to include a greater
number of water quality parameters and to require a greater frequency of parameter sampling. As indicated

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earlier in Section 6.4, periodic (e.g. monthly) extensive re-evaluation of the feed water supply is
recommended using all parameters listed in Table 1 to ensure that the feed water is of consistent quality.
Such extensive evaluation is prudent to guard against possible sudden changes in feed water quality.

Sample collection frequency and protocol shall be defined explicitly by the FTO in the PSTP. However, to
the extent possible, analyses for inorganic water quality parameters shall be performed on water sample
aliquots that were obtained simultaneously from the same sampling location, in order to ensure the maximum
degree of comparability between water quality analytes.

8.5 Evaluation Criteria

•	Nitrate Removal

The primary BD system evaluation criterion is the nitrate removal capacity expressed as the amount of
nitrate removed per unit reactor volume per unit time (e.g. lb N03-N/ft3-hr) claimed by the
manufacturer for the application being verified.

Provide a graph showing the BD influent and product water nitrate concentrations as a function of
elapsed operating time.

•	Fouling

As indicated earlier, depending on raw water quality, biological denitrification can result in additional
byproducts of nuisance and/or fouling potential. Detail must be paid to careful operation to minimize
the occurrence and production of such materials. Optimizing the BD reactor operating conditions
should result in minimization of these substances in the reactor effluent.

Provide graphs showing the levels of contaminants in the raw water and treated water supplies as a
function of elapsed time including dissolved and suspended solids, residual organic carbon source
concentrations, and hydrogen sulfide.

9.0	TASK 4: PROCESS AND EQUIPMENT MAINTENANCE

9.1	Introduction

Under this task, the FTO shall demonstrate that adequate steps are being taken to insure the continued
maintenance of both the biological denitrification process as well as the BD process equipment. The
process maintenance schedule shall delineate steps to be taken to preserve process stability while
maximizing the nitrate removal rate and minimizing the extent of potential fouling of the product water quality.
Process maintenance must involve at minimum:

•	Target nitrate removal rates are met while maintaining potential nitrite concentration at desired
levels,

•	Production of minimal concentrations of residual organic carbon,

•	Production of minimal concentrations of suspended and dissolved organic solids of cellular or
extracellular origins, and

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• Minimization of the production of substances that can cause, or contribute taste, color, and/or
odor in the treated water.

The process equipment schedule shall outline steps to be taken to insure the continued optimum functioning

of all process equipment including chemical dosing, process control and monitoring equipment.

9.2	Objectives

The objectives of this task are as follows:

•	Outline steps to evaluate and maintain the continued effectiveness of the biological process in
removing nitrate while reducing the potential production of fouling substances.

•	Confirm that Manufacturer-recommended equipment management schedules (Manufacturer
Operations and Maintenance [O&M] Manual) are sufficient to maintain the continued functional
integrity of all process control and monitoring, and that procedures and methods to restore the
integrity of such equipment upon malfunction, are current.

9.3	Work Plan

9.3.1	Process Maintenance

The FTO shall ascertain that:

1.	Organic carbon dosing equipment is set to correspond to stoichiometric limits dictated by
the influent feed water nitrate concentration and the influent water DO concentration.

2.	Reactor environmental conditions are maintained at optimal levels with respect to pH,
temperature, and adequate supply of essential trace elements and nutrients.

3.	The attached and suspended solids inventories in the reactor system are monitored on a
regular and continuous basis and that excess biological solids are removed by draining or
backwashing, or both. A reactor backwash procedure based on the type(s) of biomass
support matrix characteristics (packing density, porosity, and specific surface area) should be
maintained. When possible, the frequency of backwash should be established to allow for
better process automation.

9.3.2	Equipment Maintenance

Regular schedule and O&M manuals for equipment testing, calibration mechanical maintenance and

replacement and/or repair are required. The following are recommended criteria for evaluation of

O&M manuals for BD treatment systems.

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9.3.2.1 Operation. Provide clear and concise recommendations for procedures related to proper
operation of the BD treatment system and equipment. Include as a minimum, information on the
following:

•	Startup

Initial startup of system including reactor seeding
Establishment of steady state operation

Restart and possible reseeding of the reactor system after prolonged shutdown

•	Shutdown and biomass inventory management

Short term shut down (one day or less)

Intermediate term (one day to one week)

Long term (more than one week)

•	Backwash Procedures

Backwash cycle details including duration
Backwash frequency

•	Chemical Feed Systems

All chemicals with anticipated use
Dosing rates

Automation of chemical control system (e.g., pH, control of carbon source feed)

•	Tolerance of the system to operating conditions

Feed water temperature
pH

Oxidants (e.g., dissolved oxygen, chlorine, etc.)

Maximum feed pressure and maximum allowable differential pressure across each stage of the
reactor system

•	Adjustment to operating parameters

Influent water flow rates

Influent water nitrate and DO concentrations

9.3.2.2 Maintenance. Provide clear and concise procedures for performing maintenance on the
system and its components.

•	Instructions for installing or replacing system control and process monitoring equipment and
components.

•	Recommended or required maintenance schedules for each piece of equipment.

•	A list of spare parts to be kept on hand.

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9.3.2.3 Troubleshooting. Provide clear and concise procedures for troubleshooting.

•	Provide an explicit list of alarm conditions that the system must respond to:

Pressure
pH

Pump Failure

Chemical feed low tank level

•	Indicate which alarm conditions will cause automatic system shutdown and provide instructions
for clearing each condition.

•	Provide detailed procedures for verifying integrity of the reactor system, back-flow prevention,
all flow control and check valves, etc. on a vessel-by-vessel basis.

10.0	TASK 5: DATA REDUCTION AND PRESENTATION

10.1	Introduction

The data management system used in the verification testing program shall involve the use of computer
spreadsheet software, manual recording methods, or both, for recording operational parameters of the BD
equipment on a daily basis.

10.2	Objectives

The objectives of this task are as follows:

•	Establish a viable structure for the recording and transmission of field testing data such that the
FTO provides sufficient and reliable operational data for verification purposes.

•	Develop a statistical analysis of the data, as described in 'EPA/NSF ETV Protocol For
Equipment Verification Testing For Removal Of Nitrate: Requirements For All Studies"
(Chapter 1).

10.3	Work Plan

The following protocol has been developed for data handling and data verification by the FTO. Where
possible, a Supervisory Control and Data Acquisition (SCADA) system should be used for automatic entry
of testing data into computer databases. Specific parcels of the computer databases for operational and
water quality parameters should then be downloaded by manual importation into a spreadsheet software as
a comma delimited file. These specific database parcels should be identified based upon discrete time spans
and monitoring parameters. In spreadsheet format, the data should be manipulated into a convenient
framework to allow analysis of equipment operation. Backup of the computer databases should be
performed on a daily basis, if possible.

In the case when a SCADA system is not available, field testing operators shall record all data and
calculations by hand in laboratory notebooks (daily measurements shall be recorded on specially-prepared
data log sheets, as appropriate). The laboratory notebook should provide carbon copies of each page.

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The original notebooks shall be stored on-site; the carbon copy sheets should be forwarded to the project
engineer of the FTO at least once per week. This protocol should not only ease referencing the original
data, but offer protection of the original record of results. Operating logs shall include a description of the
BD equipment (description of test runs, names of visitors, description of any problems or issues, etc.); such
descriptions shall be provided in addition to experimental calculations and other items.

The database for the project shall be set up in the form of custom-designed spreadsheets. The
spreadsheets shall be capable of storing and manipulating each monitored water quality and operational
parameter from each task, each sampling location, and each sampling time. All data from the laboratory
notebooks and data log sheets shall be entered into the appropriate spreadsheet. Data entry should be
conducted on-site by the designated field testing operators. All recorded calculations should also be
checked at this time. Following data entry, the spreadsheet should be printed out and the printout is
checked against the handwritten data sheet. Any corrections shall be noted on the hard copies and
corrected on the screen, and then a corrected version of the spreadsheet should be printed out. Each step
of the verification process shall be initialed by the field testing operator or engineer performing the entry or
verification step.

Each experiment (e.g. each test run) shall be assigned a run number which will then be tied to the data from
that experiment through each step of data entry and analysis. As samples are collected and sent to
analytical laboratories that are certified, accredited or approved by a State, a third-party organization (i.e.
NSF), or the U.S. EPA, the data shall be tracked by use of the same system of run numbers. Data from the
outside laboratories shall be received and reviewed by the field testing operator. These data shall be
entered into the data spreadsheets, corrected, and verified in the same manner as the field data.

11.0	TASK 6: QUALITY ASSURANCE/QUALITY CONTROL

11.1	Introduction

Quality assurance and quality control of the operation of the BD equipment and the measured water quality
parameters shall be maintained during the verification testing program.

11.2	Objectives

The objective of this task is to maintain strict QA/QC methods and procedures during the Equipment
Verification Testing Program. Maintenance of strict QA/QC procedures is important, in that if a question
arises when analyzing or interpreting data collected for a given experiment, it would be possible to verily
exact conditions at the time of testing.

11.3	Work Plan

Equipment flow rates and associated signals should be verified and verification recorded on a routine basis.
A routine daily walk through during operation shall be established to verily that each piece of equipment or
instrumentation is operating properly. Particular care shall be taken to verify that chemicals are being fed at
the defined flow rate into a flow stream that is operating at the expected flow rate, such that the chemical
concentrations are correct. In-line monitoring equipment such as flow meters, etc. shall be checked to
verify that the readout matches with the actual measurement (i.e. flow rate) and that the signal being

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recorded is correct. The items listed are in addition to any specified checks outlined in the analytical
methods.

11.3.1	Daily QA/QC Verifications

•	Chemical feed pump flow rates (verified volumetrically over a specific time period).

•	On-line turbidimeter flow rates (verified volumetrically, if employed).

•	On-line turbidimeter readings checked against a properly calibrated bench model, if employed.

11.3.2	Weekly QA/QC Verifications

•	In-line flow meters/rotameters (clean equipment to remove any debris or biological buildup and
verify flow volumetrically to avoid erroneous readings).

•	Recalibration of on-line pH meters and/or conductivity meters, if used.

11.3.3	QA/QC Verifications Performed Before Each Test Period

•	On-line turbidimeters (clean out reservoirs and recalibrate, if employed).

•	Differential pressure transmitters, if used (verify gauge readings and electrical signal using a
pressure meter).

•	Tubing (verify good condition of all tubing and connections, replace if necessary).

11.3.4	On-Site Analytical Methods

The analytical methods utilized in this study for on-site monitoring of raw feed water and product
water quality are described in the sections below. Use of either bench-top or on-line field analytical
equipment should be acceptable for the verification testing; however, on-line equipment is
recommended for ease of operation. Use of on-line equipment is also preferable because it
reduces the introduction of error and the variability of analytical results generated by inconsistent
sampling techniques.

11.3.4.1	pH. Analyses for pH shall be performed according to Standard Method 4500-H. A
three-point calibration of the pH meter used in this study shall be performed once per day when the
instrument is in use. Certified pH buffers in the expected range shall be used. The pH probe shall
be stored in the appropriate solution defined in the instrument manual.

11.3.4.2	Turbidity. During each verification testing period, the in-line and bench-top
turbidimeters shall be left on continuously. Once each turbidity measurement is complete, the unit
shall be switched back to its lowest setting. All glassware used for turbidity measurements shall be
cleaned and handled using lint-free tissues to prevent scratching. Sample vials shall be stored
inverted to prevent deposits from forming on the bottom surface of the cell.

The Field Testing Organization shall be required to document any problems experienced with the
monitoring turbidity instruments, and shall also be required to document any subsequent
modifications or enhancements made to monitoring instruments.

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Bench-top Turbidimeters. Grab samples shall be analyzed using a bench-top turbidimeter.
Readings from this instrument shall serve as reference measurements throughout the study. The
bench-top turbidimeter shall be calibrated within the expected range of sample measurements at the
beginning of verification testing and on a weekly basis using primary turbidity standards of 0.1,0.5,
and 3.0 NTU. Secondary turbidity standards shall be obtained and checked against the primary
standards. Secondary standards shall be used on a daily basis to verily calibration of the
turbidimeter and to recalibrate when more than one turbidity range is used.

The method for collecting grab samples shall consist of running a slow, steady stream from the
sample tap, triple-rinsing a dedicated sample beaker in this stream, allowing the sample to flow
down the side of the beaker to minimize bubble entrainment, double-rinsing the sample vial with the
sample, carefully pouring from the beaker down the side of the sample vial, wiping the sample vial
clean, inserting the sample vial into the turbidimeter, and recording the measured turbidity. For the
case of cold water samples that cause the vial to fog preventing accurate readings, the vial shall be
allowed to warm up by partial submersion in a warm water bath for approximately 30 seconds.

In-line Turbidimeters. In-line turbidimeters shall be used for measurement of turbidity in the
filtrate water during verification testing and must be calibrated and maintained as specified in the
manufacturer's operation and maintenance manual. It will be necessary to verily the in-line readings
using a bench-top turbidimeter at least daily; although the mechanism of analysis is not identical
between the two instruments, the readings should be comparable. Should the comparison suggest
inaccurate readings, then all in-line turbidimeters should be recalibrated. In addition to calibration,
periodic cleaning of the lens should be conducted, using lint-free paper, to prevent any particle or
microbiological build-up that could produce inaccurate readings. Periodic verification of the sample
flow should also be performed using a volumetric measurement. Instrument bulbs should be
replaced on an as-needed basis. It should also be verified that the LED readout matches the data
recorded on the data acquisition system, if the latter is employed.

11.3.5 Chemical and Biological Samples Shipped Off-Site for Analysis

TOC and UV absorbance samples shall be collected in glass bottles supplied by the laboratory
(certified, accredited or approved by a State, a third-party organization (i.e. NSF), or the U.S.
EPA) and shipped at 4ฐC to the analytical laboratory within 8 hours of sampling. The TOC and
ultraviolet (UV) absorbance samples shall be collected and preserved in accordance with Standard
Method 5010B

Inorganic chemical samples, including alkalinity, hardness, iron, and manganese, shall be collected
and preserved in accordance with Standard Method 301 OB, paying particular attention to the
sources of contamination as outlined in Standard Method 3010C. The samples should be
refrigerated at approximately 2 to 8ฐC immediately upon collection, shipped in a cooler, and
maintained at a temperature of approximately 2 to 8ฐC. Samples shall be processed for analysis by
a laboratory that is certified, accredited or approved by a State, a third-party organization (i.e.
NSF), or the U.S. EPA within 24 hours of collection. The laboratory shall keep the samples at
approximately 2 to 8ฐC until initiation of analysis.

Samples for analysis of Total Coliforms (TC) and Heterotrophic Plate Counts (HPC) shall be
collected in bottles supplied (or approved) by the qualified laboratory and shipped with an internal

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cooler temperature of approximately 2 to 8ฐC to the analytical laboratory. Samples shall be
processed for analysis by the qualified laboratory within 24 hours of collection. TC densities are
reported as most probable number per 100 milliliters (MPN/100 mL) and HPC densities are
reported as colony forming units per milliliter (cfu/mL).

12.0 REFERENCES

American Public Health Association, (APHA), Standard Methods for the Examination of Water and
Wastewater, 20th Edition, 1999, APHA, Washington, DC.

Dahab, M.F. and W.L. Woodbury, 1998, "Biological Treatment Options for Nitrate Removal From
Drinking Water," Proceedings of the AWWA Inorganic Contaminants Workshop, San Antonio, TX,
Feb 22-24.

Dahab, M.F. and J. Kalagiri, 1996, Nitrate removal from water using cyclically operated fixed-film bio-
denitrification reactors, Water Science and Technology, 34, 1-2, 331-338.

Dahab, M.F., G.A. Guter, and F. Rogalla, 1991, Experience with nitrate treatment in the United States and
Europe, Proceedings, Annual Conference, American Water Works Association, Philadelphia, PA, June 23-
27.

Gayle, B. P., G. D. Boardman J. H. Sherrard and R. E. Benoit, 1989, Biological denitrification of water,
Journal of Environmental Engineering, 115, 5.

Rogalla, F., G. de Larminat, J. Coutelle, and H. Godart, 1990, Experience with nitrate removal methods
from drinking water, in:"Nitrate Contamination: Exposure, Consequences and ( \)nlrof\ NATO ARW
Series G, Vol. 30,1. Bogardi and R.D. Kuzelka, Editors, Springer-Verlag Publishers.

Siddique, M.A. and J.C. Young, 1995, Denitrification using a two-stage cyclic process. Proceedings of the
68th Annual Conference of the Water Environment Federation, Miami, FL, Oct. 21-25.

U.S. Environmental Protection Agency, 1996, ICR Manual for Bench and Pilot-Scale Treatment Studies,
Office of Ground Water and Drinking Water, Cincinnati, Ohio.

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