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EPA/63 0/P-02/004A
October 2002
External Review Draft
www.epa.gov/ncea/raf

Generic Assessment Endpoints for Ecological Risk Assessments

NOTICE

THIS DOCUMENT IS A PRELIMINARY DRAFT. It has not been formally
released by the U.S. Environmental Protection Agency and should not at this
stage be construed to represent Agency policy. It is being circulated for
comment.

Risk Assessment Forum
U.S. Environmental Protection Agency
Washington, D.C. 20460


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DISCLAIMER

This document is a draft for external review purposes only and does not constitute U.S.
Environmental Protection Agency Policy. Mention of trade names or commercial products does
not constitute endorsement or recommendation for use.

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CONTENTS

PREFACE 	v

AUTHORS AND REVIEWERS	 vii

1.	INTRODUCTION: PURPOSE OF THIS DOCUMENT	1

1.1.	Definitions	1

1.2.	Potential Uses for a Set of Generic Endpoints	2

1.3.	Criteria for GEAEs 	4

2.	EPA's INITIAL SET OF GENERIC ECOLOGICAL ASSESSMENT ENDPOINTS 	6

2.1.	Definitions of the GEAEs 	8

2.2.	Assessment Populations and Communities	12

3.	HOW TO USE THE GEAES	20

3.1.	Choosing From the Set	20

3.2.	Making the Generic Endpoints Specific 	21

3.3.	Adding Other Ecological Assessment Endpoints 	22

3.4.	Completing the List of Assessment Endpoints 	22

4.	RECOMMENDATIONS FOR FURTHER PROGRESS 	23

4.1.	Developing a Continual, Open Process for Reviewing and Amending GEAEs ... 23

4.2.	Keeping Track of New Rationales and Precedents Used in Ecological Risk

Assessment and Management Decisions	23

4.3.	Potential GEAEs for Future Consideration	24

5.	CONCLUSION 	26

APPENDIX A. SUPPORTING INFORMATION	27

A.l. Organism-Level Endpoints 	28

A.2. Population-Level Endpoints	37

A.3. Community-Level Endpoints	41

A.4. Ecosystem and Location-Specific Endpoints	44

APPENDIX B. TYPES OF VALUES ASSOCIATED WITH ASSESSMENT ENDPOINTS

	55

REFERENCES 	59

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LIST OF TABLES

2.1.	Generic Ecological Assessment Endpoints 	7

2.2.	Generic Ecological Assessment Endpoints: summary of the policy support for their use and

their practicality	15

4.1. Example scales to consider in developing assessment endpoints 	25

LIST OF TEXT BOXES

1-1. Overlap of GEAEs	2

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PREFACE

Ecological risk assessment is a process for evaluating the likelihood that adverse
ecological effects may occur or are occurring as a result of exposure to one or more stressors. A
critical early step in conducting an ecological risk assessment is deciding which aspects of the
environment will be selected for evaluation. This step is often challenging because of the
remarkable diversity of species, ecological communities, and ecological functions from which
those involved in risk assessment can choose and because of statutory ambiguity regarding what
is to be protected. The purpose of this document is to build on existing EPA guidance and
experience to assist those who are involved in ecological risk assessments in carrying out this
step, which is termed "selecting assessment endpoints" in the parlance of ecological risk
assessment. The document describes a set of endpoints, known as Generic Ecological
Assessment Endpoints, that can be considered and adapted for specific ecological risk
assessments.

This document was prepared by a Technical Panel under the auspices of EPA's Risk
Assessment Forum. The Risk Assessment Forum was established to promote scientific
consensus on risk assessment issues and to incorporate this consensus into appropriate risk
assessment guidance. To accomplish this, the Forum assembles experts from throughout EPA in
a formal process to study and report on these issues from an Agency-wide perspective. The
document reflects the Forum's long-standing commitment to advancing ecological risk
assessment and is intended to supplement the use of the Forum's Guidelines for Ecological Risk
Assessment (U.S. EPA, 1998a). Following the publication of the Guidelines, the Forum surveyed
ecological risk assessors from across the Agency to prioritize and select risk assessment topics
for further development. Additional guidance on assessment endpoints emerged as one of the
highest priority topics. A subsequent EPA colloquium sponsored by the Forum to consider high
priorities from the survey identified a need for Agency-wide generic ecological assessment
endpoints and directly led to the development of this document.

A goal of this document is to enhance the application of ecological risk assessment at
EPA, thereby improving the scientific basis for ecological risk management decisions. However,
the document is not a regulation nor is it intended to substitute for federal regulations. It

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describes general principles and is not prescriptive. Rather, it is intended to be a useful starting
point that is flexible enough to be applied to many different types of ecological risk assessments.
Risk assessors and risk managers at EPA are the primary audience; the document also may be
useful to others outside the Agency.

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AUTHORS AND REVIEWERS

This report was prepared by a Technical Panel of EPA scientists under the auspices of
EPA's Risk Assessment Forum.

Technical Panel

Glenn W. Suter II (Chair)

National Center for Environmental

Assessment
Office of Research and Development
U.S. EPA

Cincinnati, OH 45268
Thomas Gardner

Office of Science and Technology
Office of Water
U.S. EPA

Washington, DC 20460
Donald J. Rodier

Office of Pollution Prevention and Toxics
Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460

Michael E. Troyer

National Center for Environmental

Assessment
Office of Research and Development
U.S. EPA

Cincinnati, OH 45268

Douglas J. Urban

Office of Pesticide Programs

Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460

Maijorie C. Wellman

Office of Science and Technology

Office of Water

U.S. EPA

Washington, DC 20460
Steven Wharton

Office of Partnerships and Regulatory

Assistance
Region 8
U.S. EPA
Denver, CO 80202

James White

Office of Air Quality Planning and

Standards
Office of Air and Radiation
U.S. EPA

Research Triangle Park, NC 27711
Risk Assessment Forum Staff

Patti Lynne Tyler

Office of Technical and Management

Services
Region 8
U.S. EPA
Denver, CO 80202

Scott Schwenk

National Center for Environmental

Assessment
Office of Research and Development
U.S. EPA

Washington, DC 20460

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REVIEWERS

Jim Carleton

Office of Pesticide Programs
Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460
Bruce Duncan

Office of Environmental Assessment
Region 10
U.S. EPA
Seattle, WA98101

Anne Fairbrother
Western Ecology Division
National Health and Environmental

Effects Research Laboratory
Office of Research and Development
U.S. EPA

Corvallis, OR 97333
Gina Ferreira

Division of Environmental Planning and

Protection
Region 2
U.S. EPA

New York, NY 10007-1866
Laura Gabanski

Office of Wetlands, Oceans, and Watersheds
Office of Water
U.S. EPA

Washington, DC 20460

Jim Goodyear

Office of Pesticide Programs
Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460
Amuel Kennedy

Office of Pollution Prevention and Toxics
Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460
Wayne Munns

National Health and Environmental

Effects Research Laboratory
Office of Research and Development
U.S. EPA

Narragansett, RI 02882
Deirdre Murphy

Office of Air Quality Planning and

Standards
Office of Air and Radiation
U.S. EPA

Research Triangle Park, NC 27711
J. Vincent Nabholz

Office of Pollution Prevention and Toxics
Office of Prevention, Pesticides, and

Toxic Substances
U.S. EPA

Washington, DC 20460

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Vicki Sandiford

Office of Air Quality Planning and

Standards
Office of Air and Radiation
U.S. EPA

Research Triangle Park, NC 27711
Mike Slimak

National Center for Environmental

Assessment
Office of Research and Development
U.S. EPA

Washington, DC 20460

Barbara M. Smith

Policy and Management Division

Region 9

U.S. EPA

San Francisco, CA 94105
Sharon Thorns

Waste Management Division
Region 4
U.S. EPA

Atlanta, GA 30303-8960

Amy Vasu

Office of Air Quality Planning and

Standards
Office of Air and Radiation
U.S. EPA

Research Triangle Park, NC 27711

Randall S. Wentsel

Office of Science Policy

Office of Research and Development

U.S. EPA

Washington, DC 20460
Jordan M. West

National Center for Environmental

Assessment
Office of Research and Development
U.S. EPA

Washington, DC 20460

Molly R. Whitworth

Office of Science Policy

Office of Research and Development

U.S. EPA

Washington, DC 20460

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1. INTRODUCTION: PURPOSE OF THIS DOCUMENT

Assessment endpoints are used in the process of ecological risk assessment (U.S. EPA,
1998a). They represent valued attributes of ecological entities upon which risk-management
actions are focused. Since not all organisms or ecosystem features can be studied, regulatory
agencies and other risk managers must choose from among many candidates. A recommendation
for improving ecological risk assessment and management within EPA has been to develop a set
of generic assessment endpoints that are based on environmental legislation, EPA's policies and
precedents, and which cover EPA's range of concerns for the protection of ecological resources
and functions.

The Guidelines for Ecological Risk Assessment provide three selection criteria for
assessment endpoints - ecological relevance, susceptibility (exposure plus sensitivity), and
relevance to management goals. Assessment endpoints are developed during the Problem
Formulation phase of ecological risk assessment, based on input from the Planning process (U.S.
EPA, 1998a). This document presents a set of Generic Ecological Assessment Endpoints
(GEAEs) to provide examples of endpoints applicable to a wide variety of assessment scenarios
and guidance for using these GEAEs to develop robust, assessment-specific endpoints.

1.1. Definitions

An assessment endpoint is defined as "an explicit expression of the environmental value
to be protected, operationally defined as an ecological entity and its attributes" (U.S. EPA,
1998a). For example, an ecological entity might be an important fish species (e.g., coho salmon),
with its attributes being fecundity and recruitment. GEAEs are endpoints that are applicable to a
wide range of ecological risk assessments (ERAs) because they reflect the programmatic goals of
the Agency, are applicable to addressing a wide array of environmental issues, and may be
estimated using existing assessment tools. Selecting appropriate assessment endpoints is a
critical step in ensuring that an assessment will be useful to risk managers in making informed
and scientifically defensible environmental decisions.

Published generic endpoints are available for regional assessments (Suter, 1990),
population assessments (Suter and Donker, 1993), assessments of hazardous waste combustors
(U.S. EPA, 1999a) and contaminated sites in Alaska (Alaska Department of Environmental

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Conservation, 2000). In addition, examples of ecological assessment endpoints evaluated within
certain EPA programs have been highlighted in prior EPA documents (U.S. EPA, 1994, 1997a,
1997c, and 1998a). These examples are presented, as appropriate, in Appendix A.

GEAEs are not a complete list of what EPA protects, or by exclusion, what it does not
protect. They are not specifically defined for every conceivable case, and some ad hoc
elaboration by users is expected (Section 3.3). Furthermore, they are not goals or objectives, but
should be related to them, when known. For example, a generic endpoint could be created for
endangered species, but the specific species of concern would be defined during problem
formulation and attributes of the species may be selected to fulfill the regulatory mandates under
the Endangered Species Act.

Text Box 1-1. Overlap of GEAEs.

GEAEs are not necessarily discrete, mutually exclusive concepts. As such, there may
be some redundancy in a set of GEAEs. For example, the condition of an ecological entity at
one level of biological organization (e.g., organism) may influence the condition of that and
interdependent entities at higher levels of biological organization (e.g., population or
community). Also, a large change in one attribute may overlap with another attribute as in the
case of abundance and extirpation. Furthermore, GEAEs may relate to more than one
environmental value (Appendix B), which may be reflected in multiple statutes, regulations,
public policies, or public perceptions (Appendix A).

1.2. Potential Uses for a Set of Generic Endpoints

The initial set of generic endpoints proposed in Section 2 of this document should be
useful for risk assessors and managers involved in planning and performing ecological risk
assessments within various EPA programs and offices. In particular, this document can be
consulted during the problem formulation stage of ecological risk assessments to assist in
developing assessment endpoints that are useful in EPA's decision making process, practical to
measure and well defined. In addition, the specific environmental laws, precedents and other
polices that provide the supporting information for this initial set of generic endpoints in
Appendix A of this document should be equally useful in supporting assessment-specific

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endpoints.

Individual programs may have specific uses for these generic endpoints beyond
ecological risk assessments. For example, the water quality management program could find this
information useful for refining designated aquatic life uses in State and Tribal water quality
standards, developing guidance for consistent and environmentally relevant monitoring
programs, and in interpreting and implementing narrative water quality standards. For example,
this initial set of generic endpoints could be used to establish an appropriate target for a Total
Maximum Daily Load for a waterbody that has been listed for non-support of aquatic life, but
where there are no numeric biocriteria in the state's water quality standard. This initial set of
generic endpoints could be used to assist in the selection of water quality indicators or to target
attributes for those ecological entities.

Ultimately, generic assessment endpoints could have several other uses within the
Agency. Briefly, those uses, described more fully by Suter (2000), are:

To give risk managers a basis for action similar to commonly employed human health
endpoints.

To provide a threshold for prevention of environmental degradation, by ensuring that
certain values are at least considered for assessment.

To comply with legal requirements.

To improve the consistency of ecological risk assessment and management.

As models for site-, action-, or region-specific endpoints.

For screening-level ecological risk assessments, which may need to rapidly develop
endpoints with little input.

To provide clear direction for the development of methods and models.

To facilitate communication with stakeholders, by creating a set of familiar and clear
generic endpoints.

To reduce the time and effort required to conduct assessments.

It is important to emphasize that the generic assessment endpoints are not mandatory or
applicable to all assessments. This particular set of generic endpoints will be used only when and
where they are relevant. It is likely that in most ecological risk assessments, generic assessment
endpoints will be supplemented by more specific endpoints relevant to the stressor or ecosystem
being assessed. Over time, EPA anticipates that this initial set of generic ecological assessment

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endpoints will be periodically reviewed, modified, and supplemented as experience is gained in
how to apply and interpret them in a variety of natural conditions and regulatory contexts
(Section 4).

1.3. Criteria for GEAEs

EPA has provided criteria for developing assessment-specific assessment endpoints (U.S.
EPA, 1998a, Section 3.3.2). They are: ecological relevance, susceptibility, and relevance to
management goals. Ecological relevance pertains to the role of the endpoint entity in the
ecosystem and therefore depends on the ecological context. Susceptibility pertains to the
sensitivity of the endpoint to the stressor relative to its potential exposure and therefore depends
on the identity of the stressor and the mode of exposure. Relevance to management goals
pertains to the goals set by the risk manager and therefore depends on the societal, legal and
regulatory context of the risk management decision as well as the preferences of the individual
risk manager and of influential stakeholders. Hence, these situation-specific criteria are applied
whenever GEAEs are implemented in individual assessments (Section 3). They are not,
however, useful for evaluating the broad applicability of potential GEAEs. Rather, the GEAEs
presented in this report were evaluated against the following criteria, which are independent of
specific assessment situations:

1.	Generally useful in EPA's decision making process. This utility may be indicated by the
language found in statutes, treaties, and regulations that the Agency implements or with which it
must comply. Judicial decisions also indicate how the values defined by statutes may be
translated into genetically useful endpoints. In addition, Agency guidance, guidelines, protocols
and official memoranda indicate potentially useful endpoints. Finally, various actions of the
Agency that were based on ecological protection (i.e., Agency precedents) provide evidence of
general utility for GEAEs. These various sources of environmental policy are summarized in
U.S. EPA (1994, 1997a). Additional sources have been identified by the authors of this
document (Appendix A). Note that the reliance on available policy and precedent in this
document should not suggest a similar restraint on risk assessors and managers in practice. EPA
has a broad mandate to protect the environment which can support the use of novel endpoints in
individual assessments (Section 3, 4).

2.	Practical. Methods used to estimate risks to the endpoint entity and attribute should be

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available and reasonably practicable in various assessment contexts. This requires methods to
directly measure or observe the endpoint's attributes or methods to estimate them using a
combination of measurements and models. However, this does not require that a GEAE be
useful for all situations. Some GEAEs will not be implementable for some taxa or ecosystems,
but they should be practical in many situations.

3. Well defined. At a minimum, GEAEs must include an entity and an attribute of that entity
(U.S. EPA, 1998a). The entity and attribute should be clearly explained, so that they are
understandable to the public and decision makers without being ambiguous to environmental
scientists. A definition should be supported by a clear explanation of the endpoint's relationship
to the Agency's management goals and programmatic applications.

Support for the first two criteria (usefulness and practicality) is presented in Appendix A
and summarized in Table 2.2. The third criterion (that GEAEs be well defined) is supported by
the definitions in Section 2.1, supplemented by the background in Appendix A.

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2. EPA's INITIAL SET OF GENERIC ECOLOGICAL ASSESSMENT ENDPOINTS

This chapter presents EPA's initial set of Generic Ecological Assessment Endpoints to be
considered for the uses described in Section 1.2. As stated before, these GEAEs are not
exhaustive or mandatory, but are provided to assist EPA programs, researchers and decision
makers involved in protecting the Nation's ecological resources as described in Section 3. The
entities and properties comprising the initial set of GEAEs is presented in Table 2.1. They are
defined in Section 2.1, and the basis for the terms assessment community and assessment
population, which are used in the definitions, is explained in Section 2.2. Information
concerning laws, regulations and precedents, which support the selection and use of these
GEAEs is summarized in Table 2.2 and presented in Appendix A. A general discussion of the
values that are related to these GEAEs is presented in Appendix B. Other potential GEAEs that
were promising, but did not fully meet the criteria in Section 1.3, are discussed in Section 4.

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1	Table 2.1. Generic Ecological Assessment Endpoints

2

3

Entity

Attribute

4

Organisms

kills (mass mortality, conspicuous mortality)

5

6

(in an assessment population or
community)

gross anomalies



survival, fecundity, growth
(This endpoint applies particularly to threatened
and endangered species, marine mammals, bald
and golden eagles, and migratory birds.)





avoidance

7

Assessment population

extirpation





abundance





production

8

Assessment community or assemblage

species richness





abundance

9

Plant assemblage

production

10

Wetlands

area





function

11

Coral reefs

area





species richness

12

Critical habitat for threatened or

area

13

endangered species

quality

14

Endangered/Rare ecosystem types

area of the type (direct destruction or change to
another type)

15

Aquatic ecosystems

physical structure

16

Special places

ecological properties that make them special and
legally protected properties

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These GEAEs are not always biologically distinct, but the apparent overlaps are justified
in pragmatic terms. For example, the generic endpoint "population extirpation" is an extreme
case of the generic endpoint "population abundance." However, the extirpation of a population
is qualitatively different from a simple percentage loss of abundance. The implications of
reductions in fish abundance mean a loss of fishing income, but extirpation means an end to the
fishery. In addition, it is typically much easier to establish that extirpation has occurred (e.g., the
fish are no longer caught) or will occur (e.g., the trout stream will be drowned in a reservoir, or
the pH will be far beyond the lethal level), than to establish that some percentage reduction in
abundance has occurred or will occur. This difference in implications for the assessment and
decision making processes justify treating extirpation and abundance as different endpoints.
Similarly, kills of organisms have short-term effects on population abundance, but do not
necessarily have a significant or long-term effect on abundance. The methods for determining
that a kill has occurred are much simpler than determining that the abundance of a population has
changed. In addition, the effect of a kill on the public (such as concerns over odor and disease) is
not necessarily related to effects on the populations involved. For example, public response to a
fish kill may not be related to the ability of the fish populations involved to recover rapidly.
Therefore, kills are distinct from both population abundance and extirpation in terms of
assessment approaches and management implications.

2.1. Definitions of the GEAEs

Organisms - Organisms are the most distinct units of ecology, and attributes of organisms have
been the focus of the U.S. EPA's efforts to protect the environment. However, the use of
organisms as endpoints does not necessarily imply that each individual is protected. Rather,
organisms are a level of biological organization with certain attributes which may be the basis of
management decisions.

1. Kills - An event or multiple events involving numerous mortalities of organisms
within an assessment population or community. Kills may also be referred to as mass
mortality or conspicuous mortality. These events may be repeated and widespread, as in
bird kills due to pesticide applications; repeated at a location, as in fish kills due to
repeated treatment failures; or a single event, as in a seabird kill due to an oil spill. They

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may involve one or more species.

2.	Gross Anomalies - Deformities, lesions, or tumors in animals; death or necrosis of
plant leaves; or other overt physical injuries of organisms within an assessment
population or community. The occurrence of these injuries may involve one or more
species.

3.	Survival, Fecundity, or Growth - Survival (which may be reduced by direct lethality
or by sublethal effects that diminish survival probabilities), fecundity (the production of
viable young), and growth (increased mass or length) of some proportion of the animals
or plants in an assessment population or community are the basic attributes of concern for
nonhuman organisms. Species that are protected at the organism level by statute include:
a) Endangered and threatened species (i.e., those listed by the U.S. Fish and Wildlife
Service or the National Marine Fisheries Service as in danger of extinction under the
Endangered Species Act), b) Marine mammals (i.e., whales and porpoises, seals, sea
lions, and walruses, polar bears, sea otters, and manatees) which are protected by the
Marine Mammal Protection Act, c) Bald eagles and golden eagles, which are protected by
the Bald Eagle Protection Act, and d) nearly all birds in the U.S., including their eggs and
nests, which are protected by the Migratory Bird Treaty Act.

4.	Avoidance - Withdrawal of members of an assessment population from an area.
Assessment Population - A group of conspecific organisms occupying an area that has been
defined as relevant to an ecological risk assessment.

5.	Extirpation - Depletion of an assessment population to the point that it is no longer a
viable resource or may not fulfill its function in the ecosystem.

6.	Abundance - Numbers or density of individuals in an assessment population. Total
abundance or abundances by age or size classes may be used.

7.	Production - The generation of biomass or individuals in an assessment population
due to survival, fecundity, or growth.

Assessment Community or Assemblage - A multispecies group of organisms occupying an
area that has been defined as relevant to an ecological risk assessment. The group may include
all organisms in the area, in a taxon (a plant community or bird community), or in certain
samples (macroinvertebrates in Hester-Dendy samples).

8.	Species Richness - The number of species or of native species in an assessment

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community or assemblage.

9.	Abundance - The number of individuals in an assessment community or assemblage.
Total abundance or relative abundances of individual species or other taxa may be used.

10.	Plant Production - The generation of biomass or individuals in an assessment
assemblage of plants. The assemblage may include all plants in an area or water body, in
a taxon (e.g., flowering plants), or other definition (e.g., phytoplankton or aboveground
herbs).

Wetlands - Ecosystems transitional between terrestrial and aquatic systems where the water
table is usually at or near the surface or the land is covered by shallow water. Wetlands generally
include swamps, marshes, bogs, and similar areas. (This definition is derived from the more
comprehensive definitions of wetlands provided in National Research Council, 1995; U.S. Fish
and Wildlife Service, 1979; and U.S. Army Corps of Engineers, 1984.)

11.	Area of Wetlands - The area may be defined simply as surface extent of wetlands of
any type, but more often is defined as extent of a particular type (e.g., Atlantic white
cedar bog) or a particular category (e.g., palustrine).

12.	Function of Wetlands - Processes performed by wetlands that are services to
humans or other ecological entities. Specific functional attributes may include: water
storage, maintenance of high water tables, nutrient retention and cycling, sediment
retention, accumulation of organic matter, and maintenance of habitats for wetland
dependent plants and animals.

Coral Reefs - Marine ecosystems characterized by hydrozoan corals and associated flora and
fauna.

13.	Area of Coral Reefs - Depending on the goals of the assessment, area may be
defined as the total spatial extent of the reef, the extent of living reef, the extent of
unbleached reef, or some other definition.

14.	Species Richness of Coral Reefs - The number of coral species or of species of
other assemblages in a reef.

Critical Habitat for Threatened and Endangered Species - The specific area within the
geographical area occupied by an endangered or threatened species on which are found physical
or biological features essential to the conservation of the species and which may require special
management considerations and protections (16 U.S.C. 1532(5)). Critical habitats, legally

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defined and specified by the Secretary of Interior, are listed in 50 CFR Ch. 1 Section 17.94-76.
However, habitats which are critical to a threatened or endangered species should be protected
when identified even if they are not listed.

15.	Area of Critical Habitat for Threatened and Endangered Species - The land
coverage or equivalent aquatic extent (e.g., stream kilometers) that potentially supports
the endangered or threatened species.

16.	Quality of Critical Habitat for Threatened and Endangered Species - The

suitability of the habitat to support the endangered or threatened species.

17.	Area of Endangered or Rare Ecosystem Types - Endangered or rare ecosystems are
ecosystems that are at high risk of extinction because they are rare or significantly declining due
to destruction or transformation to another type. They may be generic (e.g., old growth or virgin
forests in the conterminous U.S.) or geographically specific (e.g., Hempstead Plains grasslands
on Long Island, NY). The National Biological Survey (Noss et al., 1995) and the Association for
Biodiversity Information, for example, have compiled information on rare and endangered
ecosystem types.

18.	Physical Structure of Aquatic Ecosystems - The physical attributes or characteristics of
water bodies, including hydrological characteristics; bathymetry; bank form; sinuosity; pool and
riffle structure; bank and channel vegetation; and substrate type and composition. This endpoint
encompasses the aesthetic and other values of aquatic ecosystem structure, and not simply habitat
quality for aquatic organisms.

19.	Properties of Special Places - Special places are public and private areas of ecological or
cultural significance that are not necessarily endangered or threatened, but are important, as
revealed by laws or other actions that set them aside to sustain their unique character or natural
heritage. Examples include: World Heritage Sites; National Parks and Natural Landmarks;
Wilderness Areas; National Wildlife Refuges; National Conservation Areas; Wild and Scenic
Rivers; Estuarine and Marine Sanctuaries; private nature preserves (e.g., Nature Conservancy
Preserves and National Audubon Society Sanctuaries); and state and local parks. For a more
comprehensive list, see U.S. EPA (1991a). The ecological properties to be protected are those
that make the place special including those that are an important part of the historical or cultural
heritage of a place (e.g., shortgrass prairie at Little Bighorn National Monument). Hence, this
GEAE is relevant only to special places with ecological properties that are important to their

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designation. We would not, for example, apply this GEAE to a renovation of Grant's Tomb.

2.2. Assessment Populations and Communities

Because the conventional ecological terminology of "populations" and "communities"
presents problems in practice, this document introduces the terms "assessment population" and
"assessment community" (defined above). While ecological assessment endpoints inevitably
include population properties such as abundance and production and community properties such
as species richness, it is difficult to delineate populations and communities in the field.
Classically defined populations are discrete and interbreeding. Classically defined communities
are discrete and their constituent species are relatively consistent and interact in predictable ways.
While these classical definitions have been important to the development of genetics, evolution,
and ecology (e.g., Hardy-Weinberg equilibrium and the competitive exclusion principle), they
have always had manifest limitations in practice. More recently, ecology has become more
focused on temporal dynamics, spatial patterns and processes, and stochasticity that belie the
notion of static, independent populations. One example of this is metapopulation analysis, which
reveals that population dynamics are significantly determined by exchange of individuals among
habitat patches or differential movement across a landscape that continuously varies in suitability
(Hanski, 1999). Communities are subject to the same dynamics. For example, the species
diversity of Pacific coral reefs is apparently determined by the availability of recruits from other
reefs within 600 km (Bellwood and Hughes, 2001). If the composition of coral reefs, which
would appear to be classic discrete communities, is in fact determined by regional dynamics,
there is little chance of delimiting discrete communities in general.

Populations may be readily delimited if they are physically isolated within a broader
species range (e.g., a sunfish population in a farm pond) or if the species consists of only one
spatially discrete population (e.g., the endangered Florida panther, whose current range is
restricted almost exclusively to southwest Florida). Otherwise, population boundaries are
difficult to define because they are typically structured on multiple scales. Genetic analyses,
which are needed to define discontinuities in interbreeding frequencies and thus to delimit
populations, are not a practical option for most ecological risk assessments.

The practical problems are even greater for communities. While the members of a
population consist of a single species, it is not always clear whether a particular group of

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organisms constitutes an instance of a particular community type. This is because the species
composition of communities varies over space and time.

To protect properties such as population production or community species richness, it is
necessary to develop a pragmatic solution to these problems. An example of such a pragmatic
solution is the approach taken to inventorying and mapping biodiversity by the Nature
Conservancy and NatureServe (formerly the Association for Biodiversity Information) (Stein et
al., 2000). Since it is not feasible to define discrete populations or communities, they inventory
and map occurrences of conservation elements. These may be defined at various scales,
depending on the elements and circumstances. For example, a plant community occurrence may
be "a stand or patch, or a cluster of stands or patches." However, an occurrence of a bird species
would be defined quite differently.

We propose a similar approach for GEAEs. For individual assessments, the population
or community entities to be protected must be defined during the problem formulation stage of
risk assessment. These assessment populations and assessment communities should be defined
in a way that is biologically reasonable and supportive of the decision. For example, it would not
be reasonable to define the belted kingfishers occurring in a 20 m steam reach as an assessment
population if that reach cannot fully support one belted kingfisher pair. On the other hand, even
though its range is effectively continuous, it would not be reasonable to define the entire species
as the assessment population given that it ranges across nearly all of North America. Rather, it
may be reasonable to define the kingfishers on a watershed or a lake as an assessment population.
Assessment populations may be defined by nonbiological considerations as well. For example,
for Superfund ecological risk assessments on the Department of Energy's Oak Ridge
Reservation, populations of large terrestrial vertebrates were delimited by the borders of the
reservation (Suter et al., 1994). This definition was reasonable not only because the Superfund
site was defined as the entire reservation, but also because the reservation was large enough to
sustain viable populations of deer, wild turkey, bobcat, etc. Although the reservation is more
forested than surrounding agricultural and residential lands, the borders of the reservation are not
impenetrable and are not ecologically distinct at all points. However, the pragmatic definition
proved useful and acceptable to the parties. For similarly practical reasons, one might define an
assessment community of benthic invertebrates in the mixing zone of an effluent plume or in the
first fully mixed reach.

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1	The selection of a scale to define an assessment population or community involves a

2	tradeoff. If the area is large relative to the extent of the stressor, the effects of that stressor will

3	be diluted. However, if the area is small, the assessment population or community may be

4	significantly affected but may seem too insignificant to concern stakeholders or prompt action by

5	the decision maker. Hence, appropriate spatial scales should be determined during the problem

6	formulation stage for individual risk assessments, taking into consideration both the ecological

7	and policy aspects of the problem.

8

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Table 2.2. Generic Ecological Assessment Endpoints: summary of the policy
support for their use and their practicality.

Entitv: Attribute(s)

Policy Support

Practicality

()iy;inism-Lo\ d 1 julpoinls

Organisms (in an
assessment population or
community): kills (mass
mortality, conspicuous
mortality)

Supported by many EPA programs.
For example, EPA has restricted
pesticide use (e.g., diazinon and
carbofuran) due to incidents of bird
mortality.

Likelihood of kills from chemical
pollutants can be estimated from
toxicity testing. Incidents may be
easy or difficult to observe, but when
seen suggest a common mechanism
or stressor exerting a strong effect.

Organisms (in an
assessment population or
community):
gross anomalies

Gross anomalies in birds, fish,
shellfish, and other organisms are a
cause for public concern and have
been the basis for EPA regulatory
action and guidance (e.g., assessed at
Superfund sites, incorporated into
biocriteria for water programs).

External gross anomalies are readily
observed and are commonly included
in survey protocols for fish and
forests. They are also reported in
toxicity tests of fish, birds, mammals,
and plants.

Organisms (in an
assessment population or
community): survival,
fecundity, growth
(particularly endangered
species, marine mammals,
eagles, and migratory
birds)

Many EPA programs rely on
organism-level attributes of survival,
fecundity, and growth in assessing
ecological risks (e.g., water quality
criteria, pesticide and toxic chemical
reviews, Superfund sites). Organism-
level species protection is mandated
by the Endangered Species Act,
Marine Mammal Protection Act,
Bald Eagle Protection Act, and
Migratory Bird Treaty Act.

Results of toxicity tests of the
survival, fecundity, and growth of
organisms are abundant and often
can be extrapolated to endangered
species and other species of concern.
Information on the ranges of listed
endangered species is available
through state and federal
governments.

Organisms (in an
assessment population or
community): avoidance

EPA water quality guidance (such as
for mixing zones) indicates that
effluents should not prevent or
interfere with passage of migrating
fish and other organisms.

Avoidance can be observed, but it is
not readily predicted. Laboratory
tests exist for avoidance but have not
been required by EPA.

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Entitv: Attribute(s)

Policy Support

Practicality

1



I'opulalion-I.^ d 1 Jidpoinls



2

3

Assessment DODulation:
extirpation

EPA has taken action or provided
guidance to prevent extirpation of
local populations (e.g., assessment of
likelihood of extirpation of fish
populations due to acid rain). See
also description for assessment
population: abundance.

Extirpation can be predicted using
population viability analysis.
Demonstrating extirpation may be
easy or difficult, depending on the
conspicuousness of a species. See
also description for assessment
population: abundance.

4

5

Assessment nomilation:
abundance

Major environmental statutes
mandate protection of animals,
plants, aquatic life, and living things
generally, which can be inferred to
entail protection of populations. EPA
policies for pesticides, toxic
chemicals, hazardous wastes, and air
and water pollutants are intended to
protect assessment populations of
organisms. Mammals, birds, fish,
aquatic invertebrates, and plants are
typically assessed.

Changes in abundance may be
predicted using conventional toxicity
data (the most common approach),
statistical extrapolation models, and
population models. An example of
population modeling at EPA was an
assessment of the risks from
chloroparaffins to trout populations
under the Toxic Substances Control
Act. Measurement of abundance in
the field may be easy or difficult,
depending on the species.

6

7

Assessment nomilation:
production

See description for assessment
population: abundance. Additionally,
a number of laws are intended to
maintain production of various
economically valuable species. EPA
water (e.g., National Estuary
Program) and air programs (e.g.,
criteria pollutant standards) have
involved protecting production of
resource species populations.

Changes in production may be
predicted using conventional toxicity
data as well as from population-based
approaches. For resource species
such as tree or fish species,
production changes may be
measurable in the field but may
require long periods of observation
time.

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Entitv: Attribute(s)

Policy Support

Practicality

1



( OllllllUllll> -I.O\ d 1 iiulpoillls



2

3

4

Assessment community or
assemblage: soecies
richness or abundance

EPA water quality biocriteria
frequently incorporate measures of
community species richness and
abundance, such as part of an Index
of Biotic Integrity (IBI).

Additionally, EPA testing for
pesticides, toxic chemicals, and water
pollutants is intended to assess
impacts to communities as well as
populations and kills of organisms.
Fish, aquatic invertebrates, and
aquatic plants are often assessed.

Changes in communities can be
inferred or modeled from
conventional toxicity data.

Measuring species richness and
abundance of aquatic communities, at
least for fish and macroinvertebrate
communities, is practical and well-
established. Ecosystem models that
assess effects of toxicants on
community properties are available,
and can use data acquired from
organism-level laboratory testing, but
are not routinely applied to date.

5

6

7

Assessment community or
assemblage: plant
production

EPA water quality policies address
overproduction of aquatic plants (and
concomitant eutrophication) due to
excess input of nutrients. EPA
policies for pesticides, toxic
chemicals, water pollutants, and air
pollutants (as in the case of ozone
and acid rain) also target decreases in
production of forests or other plant
communities.

Methods for measuring plant
production are well developed for
both terrestrial and aquatic
communities. Methods for predicting
effects of nutrient addition are
relatively well-developed. Protocols
for testing plant toxicity are available
and include production metrics.

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Entitv: Attribute(s)

Policy Support

Practicality

1



Ecosystem-Level Endpoinls



2

Wetlands: area or function

Management and protection of
wetlands by EPA is supported by a
variety of federal laws, regulations,
and directives. These include the
Clean Water Act (Section 404,
dredge and fill permit program, and
several other sections), the National
Environmental Policy Act, the
Coastal Zone Management Act,
Executive Order 11990 - Protection
of Wetlands, and the federal Wetland
Delineation Manual.

Methods for delineating wetlands are
well-established (although criteria
used by EPA and other agencies are
under revision). Changes in wetland
area are therefore relatively easy to
measure. Losses of wetland function
independent of area loss generally are
not readily observable or predictable.

3

4

Coral reef: area or soccics
richness

An Executive Order (13089)
establishes policies for protection of
coral reefs. Additional support may
be found in the Coastal Zone
Management Act and the Marine
Protection, Research, and Sanctuaries
Act. Many U.S. coral reefs are
protected by state or federal
government.

The area of a coral reef is relatively
easy to determine. The species
richness of corals and some other
assemblages (e.g., fish) is also
practical to determine. Prediction of
effects of pollutants on coral reefs is
difficult due to limited toxicology
information.

5

6

7

8

Critical habitat for
threatened and endangered
suedes:
area or quality

The Endangered Species Act
specifically mandates the protection
of critical habitat for endangered
species, in addition to the species
themselves.

Information on habitat used by listed
species is available from state and
federal agencies, although critical
habitat has not been officially
designated for most listed species.
Generally it is practical to determine
effects on habitat area and quality.

9

10

11

12

Endanaered/rare
ecosvstem tvDcs: area of
the type (direct destruction
or change to another type)

Fewer EPA precedents exist for this
endpoint than many others, but a
variety of EPA programs have
considered rare ecosystems.
Superfund guidance, for example,
calls for consideration of sensitive
ecosystems, and NEPA guidance
recommends mitigation measures to
reduce adverse impacts on imperiled
ecosystems.

NatureServe (www.naturcscrvc.ora)
is a ready data source for endangered
and rare ecosystem types. Drawing
from state natural heritage programs,
it maintains data on all known
ecological communities in the United
States, ranked from critically
imperiled to secure. Area loss is
relatively straightforward to measure,
but prediction of change to another
type could be difficult.

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1

2

3

4

5

6

Entitv: Attribute(s)

Policy Support

Practicality

Aauatic ecosvstems:
physical structure

Restoring and maintaining the
physical integrity (along with the
chemical and biological integrity) of
the nation's waters is the primary
goal of the Clean Water Act. EPA
policies and monitoring guidance
under the Act include measures of
physical structure.

Protocols exist for measuring many
of the physical characteristics of
aquatic ecosystems. The impacts of
many actions (e.g., channelization,
dam construction) on the physical
structure of water bodies can be
readily predicted. Other effects (such
as hydrology changes due to land use
changes) are more difficult, but still
possible, to model.

Special places: ecological
properties that make them
special and legally
protected properties

The Clean Air Act, National
Environmental Policy Act, and other
statutes require protection of special
places such as national parks,
wilderness areas, and wildlife
refuges, and this is reflected in EPA
policies. The Clean Water Act gives
EPA a role in designating National
Estuaries and Outstanding National
Resource Waters, which receive
additional protection.

Special places and their important
ecological properties usually can be
defined readily. Ability to predict or
detect impacts to these properties will
depend on the nature of the
properties and whether impacts are
direct or indirect.

7

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3. HOW TO USE THE GEAES

When these GEAEs are used in a risk assessment for a specific site, effluent, stressor,
etc., it will be necessary to determine whether any of them are applicable to the assessment and,
if so, how they can be made specific to the case, and whether they are sufficient for the case.
These activities are performed as part of the problem formulation phase of risk assessment (U.S.
EPA, 1998a).

3.1. Choosing From the Set

The set of GEAEs is intended to be a helpful starting point for identifying and specifically
defining assessment endpoints. During problem formulation, assessment scientists, risk
managers, and any stakeholders involved may select from the set those GEAEs that are relevant
to the assessment and are of sufficient importance to potentially influence the decision. This
process should be informed by any goals that may have been set prior to the problem formulation
(U.S. EPA, 1998a). The assessment-specific criteria for selecting assessment endpoints from the
guidelines for ERA (ecological relevance, susceptibility to known or potential stressors, and
relevance to the management goals) can be used to arrive at a final list of endpoints for that
particular assessment (U.S. EPA, 1998a).

The most obvious approach to using the GEAEs is to simply review the list to determine
which GEAEs are susceptible, relevant and important to the assessment. For example, one
might consider whether any aspect of the project or stressor might result in kills (i.e.,
susceptibility) and whether the occurrence of those kills meets the criteria of ecological relevance
or relevance to management goals. Similarly, the two GEAEs for wetlands would require
consideration of whether any wetlands are potentially exposed and whether the exposure could
result in loss of area or changes in function that are potentially relevant.

Alternatively, assessment endpoints may have been suggested by stakeholders or others
by an independent process. In that case, those assessment-specific endpoints may be compared
to the set of GEAEs. If a proposed assessment endpoint corresponds to one of the GEAEs, then
the policies and precedents that support the GEAE (Appendix A) also support that proposed
assessment endpoint. For example, if stakeholders propose to protect a wetland adjoining a

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contaminated site, then the regulations and precedents that support the two GEAEs for wetlands
lend support to those stakeholder concerns as a basis for defining an assessment endpoint. If
there is no correspondence to a GEAE, then the proposed assessment endpoint may still be used,
but it must be supported for that case (Section 3.3).

3.2. Making the Generic Endpoints Specific

To convert a GEAE into an assessment endpoint for a specific assessment, it is necessary
to define the specific entity and attribute and the spatial and temporal context of the entity. This
specificity is necessary to make the endpoint relevant to the assessment and to determine what
measurements and models are needed to estimate it.

Consider the first GEAE, kills of organisms, as an example. The generic entity is
organisms. For a specific assessment endpoint, we must specify whether the endpoint entity
corresponds to members of a specific taxon such as fish or birds, an assemblage such as
macroinvertebrates, or a specific species such as sea otters. The generic attribute is kills, which
should be defined more specifically and in terms that are appropriate to the assessment. For
example, the definition of a "kill" would differ in a well-monitored experimental use of a
pesticide versus public reports of mortalities, for oil spills versus lawn treatments, and for
modeling studies versus observational studies. Possible definitions could include the number of
organisms that must die during an episode to be considered a kill, the proportion of organisms
visiting a site that would be expected to die, or the frequency of public reports of dead organisms
associated with the stressor. Finally, the spatial and temporal context should be defined. For an
effluent, the spatial context may be the downstream reach within which mixing occurs, and the
time period may be the period of a permit. For a pesticide, the spatial context may be the region
within which the pesticide is used on a particular crop and the temporal scale may refer to the
number of applications per year over the period of use. For an oil spill, the context may refer to
the area encompassed by the plume and the time until the plume is dispersed or degraded to the
point that it no longer oils marine birds or mammals. Hence, an assessment endpoint derived
from this GEAE might be: episodic mortality of several fish of any species occurring in the one
kilometer reach downstream of the effluent release point.

Note that more than one assessment endpoint may be derived from a GEAE for a
particular assessment. For example, the GEAE population abundance, may be used to generate

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assessment endpoints for each of several populations of concern and the change in abundance
and spatial context may be different for each. On the other hand, a site-specific concern may
relate to more than one GEAE. In the example of the wetland discussed in the previous section,
the site specific problem formulation must determine whether the management concern and the
evidence of wetland susceptibility are related to the area of the wetland, some functional attribute
of the wetland, or both.

3.3.	Adding Other Ecological Assessment Endpoints

The set of GEAEs presented in this document contains those that are thought to be
currently generically useful in EPA, and does not preclude the use of other endpoints. Other
endpoints may be chosen because they reflect some particular environmental value associated
with a site or held by a particular stakeholder. In addition, some endpoints that are not
generically practical may be practical in a particular case because of peculiarities of the stressor
or receptor, data availability, availability of a model of the receiving system, or because time and
resources are available to assess a difficult endpoint. Finally, the mode of exposure or mode of
action of a stressor may imply susceptibility of certain endpoints that have not been commonly
considered.

3.4.	Completing the List of Assessment Endpoints

When a list of potential assessment endpoints has been developed, it may be necessary to
review the list and reduce it to the set that is important to the decision. Because of the limitations
of time and resources, it is often advisable to limit the list of assessment endpoints to those that
are most relevant and susceptible. There is likely to be some redundancy in the endpoints. Kills
of organisms imply immediate changes in population abundance which may influence
community abundances. If population or community properties are important to the decision
maker, they should be retained. However, if kills are sufficient to warrant action, the
extrapolations to higher levels of biological organization may be unnecessary and those
endpoints may be dropped as unnecessarily redundant.

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4. RECOMMENDATIONS FOR FURTHER PROGRESS

The main purpose of this report has been to improve ecological considerations within
EPA by developing an initial set of generically useful ecological assessment endpoints. These
assessment endpoints are based on existing policy and practice rather than an evaluation of all the
potentially useful ecological assessment endpoints that may exist. In looking toward the future,
readers of this report are encouraged to develop and maintain a continual, open process for
reviewing and amending these GEAEs, and to establish a means of keeping track of the many
rationales and precedents used for making ecological risk-based decisions throughout EPA. The
remainder of this chapter provides a discussion about these two recommendations and presents
potential GEAEs for future consideration.

4.1.	Developing a Continual, Open Process for Reviewing and Amending GEAEs

The initial GEAEs presented in this report include important ecological attributes to
consider when conducting ecological assessments throughout EPA. However, the Agency
should not remain static nor constrain itself to the past. EPA should institute an adaptive,
ongoing, and open process of reviewing and amending this initial set of generic endpoints over
time as Agency experience and science evolve. The mechanism and frequency of these reviews
must be established by Agency management, but the members of this panel believe that five year
intervals would be appropriate and that broad participation is vital. Members of the reviewing
panel should represent as many programmatic, regional, and support offices of EPA as possible
and this process should be open to stakeholder input.

4.2.	Keeping Track of New Rationales and Precedents Used in Ecological Risk Assessment
and Management Decisions

A more efficient means of recording and referencing how EPA is breaking new ground in
ecological protection needs to be established. As such, the Agency should formally document
assessment endpoints used in ecological risk assessments on an ongoing basis. Where a program
or regional office finds scientific and societal justification for an assessment endpoint, the office
should consider it again in future assessments, and share this knowledge with other offices
throughout the Agency. We suggest that a central, web-based database would greatly facilitate

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this process.

4.3. Potential GEAEs for Future Consideration

An important question the technical panel preparing this report considered was whether to
restrict the list of GEAEs to assessment endpoints for which Agency precedent existed, or if new
endpoints (without EPA precedent) should be considered as well. Because the technical panel
was primarily scientific in nature and lacked authority to establish new Agency policy, the panel
decided that all the initial generic endpoints should have some existing legal or regulatory basis
or other precedent within EPA. Such precedents, as presented in Appendix A, include treaties,
statutes, regulations, judicial decisions, official memoranda, guidance or procedures, and others
precedents. Nevertheless, the technical panel was concerned that otherwise valid and important
ecological endpoints were excluded and felt it important to encourage Agency progress and
innovation in this area. In that light, potential GEAEs meriting consideration by EPA include the
following:

Nutrient cycling in ecosystems other than wetlands.

Soil productivity.

Other soil functions (e.g., nutrient retention, organic matter decomposition).

Physical structure of terrestrial ecosystems.

Landscape characteristics (e.g., extent, pattern, and diversity).

Attributes of wetlands besides area or functional capacity (e.g., stability, resilience,

diversity).

Behavior other than avoidance, such as courtship behavior or migratory behavior (e.g., in

birds and salmonids), or lapses in nurturing and rearing (e.g., nest abandonment).

Riparian system area or function.

Ecosystem attributes that influence public health.

We encourage EPA's program and regional offices to regularly consider these and other relevant
assessment endpoints for EPA's evolving ecological mission. One suggested method for
deriving other potential endpoints is to consider the many dimensions associated with ecological
systems (Table 4.1). The initial GEAEs presented in this report incorporate or touch upon many
of these dimensions, but many other endpoints could be derived by increasing the range, or

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1	integrating two or more of these dimensions in a new way.

2

3

4	Table 4.1. Example scales to consider in developing assessment endpoints.

5

6

1)

Levels of biological organization (e.g., ranging from DNA to
ecosystems, landscapes and beyond).

2)

Spatial scale (e.g., ranging from local to global).

3)

Temporal scale (e.g., considerations of the timing, duration and/or
frequency of biological activities or events).

4)

Magnitude (e.g., the total number of ecological entities present,
impacted or remaining).

5)

Less often considered taxonomic groups (i.e., beyond mammals, fish
and birds to other taxa such as amphibians, reptiles, invertebrates,
fungi, flowering and nonflowering plants, etc.).

6)

Conceptually broader range of ecological properties of concern (e.g.,
resiliency in ecosystems).

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5. CONCLUSION

While the legislative mandates to protect public health and the environment are equal in
their lack of specificity, the development of endpoints for human health risk assessment has
proceeded more expeditiously. Health risk assessments began with cancer risks and evolved to
include a range of noncancer endpoints such as birth defects, kidney function, intellectual
development, and infertility. This is due in large part to the fact that the public and Agency
scientists and managers can readily understand and personally relate to those health endpoints. In
contrast, many ecological endpoints are not as well understood by non-ecologists and lack the
same emotional immediacy. As a result, ecological endpoints have been slower to develop and
have often not been as clearly or consistently defined as are health endpoints (U.S. EPA, 1994,
1997a, 1998a). This set of GEAEs should help to remedy that situation by defining ecological
endpoints that have broad utility in the Agency and may be used by risk assessors and risk
managers with confidence that they are supported by regulations and precedents.

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APPENDIX A. SUPPORTING INFORMATION

This appendix serves as a reference for those who need to know the basis for a particular
GEAE defined in Section 2. The GEAEs have been divided into four categories of biological
organization: organism, population, community, and ecosystem. Each category is introduced by
general information about how the GEAEs in that category have been used by the Agency.
Additional supporting information is then provided for each GEAE, divided into two sections.
The first is Laws, Regulations, and Precedents, which discusses the authorities that support use
of each GEAE by EPA and gives examples of Agency actions that provide a further basis for
their use. The second section is Practicality, which discusses the availability of methods to
estimate risks to the endpoint and their applicability in various risk assessment contexts. Because
assessment endpoints are defined as valued properties of the environment (U.S. EPA, 1998a),
public values associated with the GEAEs are discussed in broad terms in Appendix B.

It should be noted that the specific laws and other policies cited below are not the only
support for ecological endpoints. The many federal environmental laws and their implementing
regulations provide a general mandate for environmental protection that goes far beyond the
specific instances presented in this Appendix. In particular, the National Environmental Policy
Act of 1970 creates a broad mandate for federal agencies to protect and prevent degradation of
the environment. While nearly all environmental statutes refer to the environment as an entity to
be protected, and many refer to more specific ecological entities such as fish, wildlife, and
estuaries, few indicate an attribute to be protected or even the nature of the entity. In addition,
terms are not necessarily used in a technical way. For example, the Clean Water Act refers
repeatedly to "a balanced indigenous population of fish, shellfish and invertebrates." Clearly, the
phrase does not refer to a biological population, which is formed of members of one species.
Further, when referring to fish, does the act mean fish at the level of organism, population,
assemblage, or as a taxon? Given these ambiguities, the wording of the statutes must be
interpreted to define endpoints. The primary source of support for the following interpretations
is precedent.

The precedents and other expressions of policy discussed below are a sample of those that
have been used in assessments, guidance, protocols, and other Agency actions over the years.
Although they are derived from particular laws and regulatory contexts, they may be interpreted

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as examples of what Congress and the Agency have meant by protecting the environment. For
example, the Clean Air Act calls for specific protection of "national parks, national wilderness
areas, national monuments, national seashores, and other areas of special national or regional
natural, recreational, scenic, or historic value." This requirement can be interpreted as a mandate
to the Agency to protect those special areas from pollution, not just the threats from air pollution
that were brought to the attention of Congress.

Abbreviations and Acronyms Used in this Appendix:

CAA

Clean Air Act

CERCLA

Comprehensive Environmental Response, Compensation, and Liability Act

CFR

Code of Federal Regulations

CWA

Clean Water Act

ESA

Endangered Species Act

FR

Federal Register

NCP

National Contingency Plan

NEPA

National Environmental Policy Act

PCB

Polychlorinated Biphenyl

FIFRA

Federal Insecticide, Fungicide, and Rodenticide Act

RCRA

Resource Conservation and Recovery Act

TSCA

Toxic Substances Control Act

A.l. Organism-Level Endpoints

Major EPA statutes such as the Clean Air Act, Clean Water Act, CERCLA, FIFRA,
TSCA, and RCRA require EPA to consider and protect organism-level attributes or various taxa
of organisms including fish, birds, and plants and, more generally, animals, wildlife, aquatic life,
and living things. The toxicity information that is available to EPA in administering these
statutes is dominated by organism-level attributes such as mortality. Organism-level attributes
tend to be more practical to measure or predict than attributes at higher levels of organization for
most EPA assessments. Consequently, EPA's ecological assessments historically have focused
on organism-level endpoints.

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Note that these endpoints do not normally imply protection of each individual organism,
but rather the protection of these critical attributes of organisms within assessment populations or
communities. EPA's principles for ecological risk assessment and risk management at Superfund
sites (U.S. EPA, 1999b) illustrate a common usage of organism-level endpoints at EPA: "Except
at a few very large sites, Superfund ERAs [ecological risk assessments] typically do not address
effects on entire ecosystems, but rather normally gather effects data on individuals in order to
predict or postulate potential effects on local wildlife, fish, invertebrate, and plant populations
and communities that occur or that could occur in specific habitats at sites .... Levels [of
chemicals] that are expected to protect local populations and communities can be estimated by
extrapolating from effects on individuals and groups of individuals using a lines-of-evidence
approach." That is, we assess risks to attributes of organisms and assume that populations and
communities will be protected if they are protected. As will be described, however, certain
special categories of organisms such as endangered species and marine mammals have been
afforded protection on an individual basis.

In ecological assessments, EPA considers organism-level effects in a variety of taxa. For
example, tests required for pesticide regulation can include effects on survival, growth, and
reproduction of aquatic invertebrates, fish, birds, mammals, and both terrestrial and aquatic
plants. Effects to a similar range of taxa are considered under TSCA (Lynch et al., 1994; Zeeman
et al., 1999) and in deriving water quality criteria under the CWA. Less commonly, other taxa are
considered such as earthworms (e.g., at certain Superfund sites), honeybees (e.g., for certain
pesticides), and reptiles and amphibians.

A.l.l. GEAE #1: Kills of Organisms

A. 1.1.1 .Laws, Regulations, and Precedents

The regulation of chemicals to prevent kills of organisms, in the absence of effects on
populations or communities, has been sustained by federal courts. For example, use of the
pesticide diazinon on golf courses and sod farms was prohibited after documentation of
widespread and repeated kills of birds (U.S. EPA, 1988a). Subsequently, EPA cited continuing
bird kills as a factor in the agreement with pesticide manufacturers to phase out all outdoor
residential uses of diazinon (U.S. EPA, 2001a). Bird kills were also the basis for phasing out

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most uses of another pesticide, granular carbofuran (U.S. EPA, 1991b; Houseknecht, 1993). Kills
of birds and other wildlife in oil pits are considered evidence of "imminent and substantial
endangerment to the environment" under RCRA §7003 (U.S. EPA, 2001b). Fish kills have also
been considered a concern by EPA; for example, Region 5 considers fish kills and other excess
mortality to be obvious impacts under RCRA (U.S. EPA, 1994).

Under FIFRA reporting requirements for adverse effects of pesticides (40 CFR Part 159),
EPA categorizes kills (and other adverse incidents) involving multiple organisms as more severe
events than single organism incidents and imposes additional reporting requirements on pesticide
registrants for such events. More severe wildlife incidents are defined as those involving at least
1,000 individuals of a schooling fish species or 50 individuals of a non-schooling species; 200
individuals of a flocking bird species, 50 individuals of a songbird species, or five individuals of
a predatory species; or, for mammals, reptiles, and amphibians, 50 individuals of a relatively
common or herding species or five individuals of a rare or solitary species. (Note that incidents
involving numbers of organisms below these thresholds still must be reported, but the
requirements are different than for more severe incidents. Also note that these criteria do not
apply outside FIFRA.)

A. 1.1.2. Practicality

The likelihood of kills is relatively readily estimated using the common acute lethality
tests which generate LC50s and LD50s. The number of species involved in kills may be
estimated from species sensitivity distributions (SSDs) of LC50s or LD50s, as in the calculation
of the acute National Ambient Water Quality Criteria (U.S. EPA, 1985; Posthuma et al., 2001).
The occurrence of kills in the field may be readily observed in the cases of conspicuous
organisms and open habitats, but in other cases, such as small birds in crops or fence rows, kills
may be unobserved and difficult to document. Recently, a model has been developed to predict
the probability of bird kills for a particular use of a cholinesterase-inhibiting pesticide using
SSDs of LD50s and field studies (Mineau, 2002).

A.1.2. GEAE #2: Gross Anomalies of Organisms

A. 1.2.1. Laws, Regulations, and Precedents

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Gross anomalies in birds, fish, shellfish, and other organisms are a cause for public
concern and have been the basis for EPA regulatory action and guidance. For example, crossed
bills and other deformities in piscivorous birds are a basis for the proposed remediation of the
PCB-contaminated sediments at the Fox River/Green Bay Superfund site (Wisconsin Department
of Natural Resources, 2001; U.S. EPA, 1998b), and were a basis for the designation of the
system as an Area of Concern by the Great Lakes National Program Office (U.S. EPA, 2001c).
EPA actions to restrict the use of tributyltin as an antifoulant on boats (U.S. EPA, 1988b), as well
as the restrictions imposed by the Organotin Antifouling Paint Control Act of 1988, were
triggered by the observed induction of gross deformities in molluscs which threatened the
marketability of oysters, reduced the fecundity of the deformed organisms, and suggested the
potential for other effects. Natural resource damage regulations for CERCLA, the CWA, and the
Oil Pollution Act include gross anomalies among the designated injuries (43 CFR §11.62(f)), and
Deformities, Erosion, Lesions and Tumors in fish (DELT anomalies) are used in the biocriteria
of many state water quality standards and in Agency guidance (Yoder and Rankin, 1995; U.S.
EPA, 1996). Changes in development, which can be manifested in physical anomalies, have been
identified as an environmental effect of regulatory concern under TSCA (U.S. EPA, 1983).

Anomalies in plants and plant injuries have also been the basis for EPA action. For
example, EPA established a secondary ambient air quality standard for ground-level ozone based
in part on visible foliar injury to commercial crops and natural vegetation, stating that "foliar
injury is occurring on native vegetation in national parks, forests, and wilderness areas, and may
be degrading the aesthetic quality of the natural landscape, a resource important to public
welfare" (U.S. EPA, 1997b). EPA has also used visible injury of plants as a basis for regulating
air emissions of aluminum reduction plants and sulfuric acid production units (U.S. EPA, 1994).

A. 1.2.2. Practicality

External gross anomalies are readily observed, as are some internal anomalies with
external manifestations such as severe scoliosis or large tumors. They are commonly included in
biological survey protocols for fish and in forest health surveys. Gross anomalies are also
included as endpoint responses in some chronic tests of fish and birds.

A.1.3. GEAE #3: Survival, Fecundity, and Growth of Organisms

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As discussed in Section A.I., EPA's ecological assessments have considered effects on
survival, fecundity, and growth in a variety of taxa. While actions based on survival may be most
common, EPA has also made regulatory decisions based on effects on fecundity and growth of
organisms identified in ecological risk assessments. For example, the pesticide chlorofenapyr
was not approved by EPA based on Agency concerns over reproductive risks to birds.
Additionally, federal statutes and other precedents confer special status on particular kinds of
organisms: endangered and threatened species, marine mammals, bald and golden eagles, and
migratory birds. The remainder of this section will concentrate on the basis for the special status
of these organisms within the organism-level endpoints.

A. 1.3.1. Laws, Regulations, and Precedents

Endangered and Threatened Species

The Endangered Species Act prohibits taking of threatened or endangered species which
is defined as to "harass, harm, pursue, hunt, shoot, wound, kill, trap, capture, or collect, or to
attempt to engage in any such conduct" (16 US Code, §1532 and 50 CFR Parts 14, 17, and 23).
Under the Act, the term "species " includes "any subspecies of fish or wildlife or plants and any
distinct population segment of any species of vertebrate fish or wildlife which interbreeds when
mature." The ESA states that it is "to be the policy of Congress that all Federal departments and
agencies shall seek to conserve endangered species and threatened species" and that "Federal
agencies shall cooperate with State and local agencies to resolve water resource issues in concert
with conservation of endangered species" (16 US Code, §1531).

Hence, the provisions of the ESA are applicable to EPA actions, and both the prohibition
against harming individual members of threatened or endangered species and the affirmative
obligation to conserve those species would seem to preclude toxic effects. Additionally, the
Clean Air Act (§112) specifically requires EPA to prevent adverse effects to endangered species
in regulating hazardous air pollutants. Like other federal agencies, EPA has published regulations
and taken actions to protect endangered species. For example, EPA has consulted with the U.S.
Fish and Wildlife Service and National Marine Fisheries Service to prevent jeopardy to
endangered species, as required by the ESA, for actions such as setting water quality standards
and regulating pesticides. In these consultations, the attributes of concern have generally been

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survival, fecundity, and growth, although other attributes may be important in specific cases. The
National Contingency Plan specifies that the ESA is a federal "applicable or relevant and
appropriate requirement" (ARAR) with which Superfund remedial actions must comply under
CERCLA §121(d)(2)(A), and examples of Superfund ecological risk assessments that used
endangered species as endpoints include the Asarco Tacoma site (chinook salmon and bull trout)
(Hillman and Rochlin, 2001), the Metal Bank of America site (shortnose sturgeon) (Wentsel et
al., 1999), and the Montrose, Iron Mountain Mine, Fort Ord and Monterey Marine Sanctuary,
Camp Pendelton-Santa Margarita River, and Pearl Harbor sites (U.S. EPA, 1994).

Marine Mammals

The Marine Mammal Protection Act protects marine mammals from taking, which is
defined as "to harass, hunt, capture, or kill, or attempt to harass, hunt, capture, or kill any marine
mammal... .The term 'harassment' means any act of pursuit, torment, or annoyance which (i) has
the potential to injure a marine mammal or marine mammal stock in the wild; or (ii) has the
potential to disturb a marine mammal or marine mammal stock in the wild by causing disruption
of behavioral patterns, including, but not limited to, migration, breathing, nursing, breeding,
feeding, or sheltering" (US Code, §1362). While the Act does not specifically address toxic
effects on marine mammals, the special protection afforded these species by the act implies a
particular concern for their well-being. Also, the law clearly protects properties of marine
mammals at the organism level.

As in the case of threatened and endangered species, the NCP specifies that the Marine
Mammal Protection Act is a federal "applicable or relevant and appropriate requirement"
(ARAR) with which Superfund remedial actions must comply under CERCLA §121(d)(2)(A),
and it cites marine mammals as examples of specific natural resources to be protected under
CERCLA Part 101, §16.

Bald and Golden Eagles

Prohibited actions under the Bald and Golden Eagle Protection Act include to "take,
possess, sell, purchase, barter, offer to sell, purchase or barter, transport, export or import, at any
time or in any manner any bald eagle commonly known as the American eagle or any golden
eagle, alive or dead, or any part, nest, or egg thereof of the foregoing eagles ..." (16 US Code,

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§668). To take is defined by regulation to include: "to pursue, hunt, shoot, wound, kill, trap,
capture, or collect, or attempt to pursue, hunt, shoot, wound, kill, trap, capture, or collect" bald
eagles or golden eagles, including any "part, nest, or egg of such bird[s]" (50 CFR §10.12).

Deaths of bald eagles due to secondary poisoning were an endpoint in EPA's assessment
of granular carbofuran (U.S. EPA, 1991b), which led to the phaseout of most uses of this
pesticide. Also, EPA's ecological risk assessment for PCBs in the Hudson River included
survival, growth, and reproduction of piscivorous birds as an assessment endpoint, with the bald
eagle selected as one of the representative species of piscivorous birds (U.S. EPA, 2000a).

Birds

The Migratory Bird Treaty Act of 1918 prohibits or regulates a number of activities,
including pursuing, taking, hunting, capturing, killing, possessing, selling, transporting, or
purchasing migratory birds, including their eggs and nests (16 US Code, §703). This Act, based
originally on a treaty between the United States and Great Britain (including Canada), has since
been extended by migratory bird conventions with Mexico, Japan, and the Soviet Union. Since
nearly all species of birds native to the United. States are protected by the Act (U.S. Fish and
Wildlife Service, 2001), the endpoint may be assumed to apply to birds in general. While the
Migratory Bird Treaty Act does not specifically address toxic effects on birds, the special
protection afforded these species by the Act implies a particular concern for their well-being.
Also, the law clearly protects birds at the organism level. Furthermore, by Executive Order
13186, all federal agencies are required to "support the conservation intent of the migratory birds
conventions by integrating bird conservation principles, measures, and practices into agency
activities and by avoiding or minimizing, to the extent practicable, adverse impacts on migratory
bird resources when conducting agency actions" and "prevent or abate the pollution or
detrimental alteration of the environment for the benefit of migratory birds, as practicable"
(Clinton, 2001).

EPA policies and precedents affirm the use of survival, growth, and reproduction of birds
in ecological assessments. The NCP specifies that the Migratory Bird Treaty Act is a federal
"applicable or relevant and appropriate requirement" (ARAR) with which Superfund remedial
actions must comply under CERCLA §121(d)(2)(A), and examples of Superfund ecological risk
assessments that used birds as endpoints include the Baird and McGuire site (survival and

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reproduction of songbirds) (Menzie et al., 1992) and the United Heckathorn site (reproductive
effects on birds) (Wentsel et al., 1999). EPA's ecological risk assessment for PCBs in the
Hudson River included survival, growth, and reproduction of insectivorous birds, waterfowl, and
piscivorous birds as assessment endpoints (U.S. EPA, 2000a). EPA regulations authorize the
Agency to require pesticide registrants to submit tests on avian mortality and impaired avian
reproduction caused by pesticides. Results from these tests are used by EPA, in conjunction with
other available information, in making pesticide registration decisions. Also, EPA's involvement
in bird conservation initiatives such as Partners in Flight and the North American Bird
Conservation Initiative provides further support for using birds in assessment endpoints (U.S.
EPA, 2002).

A. 1.3.2. Practicality

Since the vast majority of standard toxicity tests determine effects on the survival,
fecundity and growth of organisms, direct toxic effects on this endpoint are readily predicted. In
addition, extrapolation models are available that can estimate effects on this endpoint for
particular organisms and exposure routes of concern based on tests conducted on other species,
life stages or exposure durations or routes.

It is rarely possible to obtain toxicity data for threatened and endangered species, but
species sensitivity distributions, intertaxa regressions, or other interspecies extrapolation models
should serve to estimate effects of these species. EPA research has confirmed that endangered
species are not inherently more sensitive to toxic effects than other species (Sappington et al.,
2001), although, from a population standpoint, they may be at greater risk due to their low
abundance.

Effects on marine mammals are relatively difficult to observe in the field. However, die-
offs of pinnipeds and cetaceans are readily observed when their conspicuous carcasses appear on
beaches. The toxicology of marine mammals is poorly known, and, for obvious reasons, they are
not included in routine toxicity testing. However, effects on all mammals are routinely estimated
from tests performed with rodents. Exposure of marine mammals is also poorly known and is
not routinely estimated even though they can accumulate high levels of persistent pollutants.

Eagles are highly conspicuous, and dead or debilitated eagles are more likely to be
reported by the public than most birds. In addition, federal, state, and private organizations

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monitor eagles at various scales. Toxic effects on eagles may be predicted from standard avian
toxicity tests or, more confidently, from tests with kestrels, with avian allometric models used to
extrapolate toxicity results to eagles.

In the case of birds generally, their biology is well known and well-developed methods
exist for surveying bird populations and communities. Both acute and chronic test protocols for
birds are available and avian toxicity data are available for most pesticides and many other
chemicals. However, because birds are highly mobile, often migratory, and often territorial, it is
usually difficult to demonstrate chronic effects on these organisms in the field.

A.1.4. GEAE #4: Avoidance by Organisms in an Assessment Population

A. 1.4.1. Laws, Regulations, and Precedents

Fish are the class of organisms for which EPA has most frequently considered avoidance
as an endpoint. Avoidance of a noxious effluent is one of the effects which may be considered in
determining whether to allow a mixing zone around a point source discharge, and if allowable,
its location and dimensions. The Clean Water Act allows mixing zones at the discretion of
States, and therefore policies regarding them vary widely from state to state. EPA guidance (e.g.,
Water Quality Standards Handbook; Technical Support Document for Water Quality-based
Toxics Control) requires that, where allowable, mixing zones must not prevent the designated
uses of the receiving waterbody from being attained. This would mean, for instance, that a
mixing zone around the effluent discharge could not be so large and noxious as to prevent
passage of migrating fish due to their avoidance of the plume. In general, avoidance by fish is not
monitored by permittees or permitting agencies around discharges or mixing zones. Rather, the
dimensions of the mixing zone are determined either by use of simulation models (which predict
the chemical concentrations or temperature at different points within the proposed mixing zone)
or by default values (e.g., 25% of 7Q10 flow). However, where there are important migratory
species (e.g., salmonids), observance of avoidance behavior can be an important factor in
determining mixing zone dimensions.

A. 1.4.2. Practicality

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observed by divers or observers in boats or by telemetric tagging. Laboratory tests exist for
avoidance of chemicals or materials in water or food, but have not been required by the Agency.
Although EPA's use of this endpoint has emphasized vertebrates, methods are available for
invertebrates as well (Slimak, 1997).

A.2. Population-Level Endpoints

As described in Section A.I., most environmental statutes authorizing EPA activities call
for protection of a diverse array of organisms. These statutes generally can be inferred to protect
population-level endpoints in addition to organism-level endpoints, even if populations are not
specifically cited by law. EPA's principles for ecological risk assessment and risk management at
Superfund sites exemplify EPA concern about population-level endpoints, stating that
"Superfund's goal is to reduce ecological risks to levels that will result in the recovery and
maintenance of healthy local populations and communities of biota" (U.S. EPA, 1999b).

Predicting population-level impacts generally is not as straightforward as estimating
organism-level effects and, as a result, explicit estimates of population effects are less common
in EPA ecological assessments. Adverse effects to organisms are often inferred to indicate risk to
populations and hence a cause for concern under certain EPA programs, however, such as at
Superfund sites. Similar inferences are made for chemical reviews under TSCA. In examining
environmental effects of concern under TSCA, an EPA position paper reviewed a number of
statutes spanning the period of 1785 to 1978 to determine what society values in the environment
(U.S. EPA, 1983). EPA concluded that such laws were passed to prevent any reduction,
degradation, or loss in the quality, quantity or utility of a resource that is valued by the public. It
also concluded that chemicals could adversely affect these resources by causing an undesirable
change in the population structure of a species by affecting rates of mortality, reproduction, or
growth and development. Thus, organism-level attributes such as mortality can be inferred to
affect population-level attributes valued by society. Less commonly, EPA prepares quantitative
estimates of population effects based on organism-level effects or other information.

A.2.1. GEAE #5: Extirpation of An Assessment Population

Extirpation can be viewed as an extreme case of a change in abundance or production of
an assessment population, and thus its selection is supported by the factors cited in Section A.2.2.

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as well as this section. Additionally, extirpation of an assessment population may have
qualitatively more significant impacts on ecological function and environmental values than
reduction in size of an assessment population. This significance is reflected in an alternative
term for this population attribute, functional extinction.

A.2.1.1. Laws, Regulations, and Precedents

Several EPA precedents exist for assessing population extirpation. For example, EPA
examined the likelihood of extirpation of fish populations in northeastern lakes under the acid
deposition program, and vetoed a permit for a dam and reservoir project under Section 404 of the
CWA based in part on projected extirpation of populations of birds of special interest (U.S. EPA,
1994). Absence of a species normally occurring in the habitat has been used as evidence of
ecological risk at Superfund sites. Where designated aquatic life uses have been specified in state
water quality standards, extirpation of a naturally occurring species may be considered as
evidence that the waterbody is not attaining its designated uses.

A.2.1.2. Practicality

Field observations to determine whether a species is present usually are not difficult to
conduct, but ease of observation depends upon the species and care must be taken in interpreting
results. Failure to observe a species that is expected to occur in low numbers even in the absence
of stressors, is subject to substantial natural fluctuations in abundance, or is inconspicuous may
not be indicative of extirpation. Demonstrating extirpation at a site also requires evidence that
the species was formerly present.

In some cases, risk of extirpation can be inferred from toxicity data. Very high exposure
in the field in comparison to exposures where toxic effects have been observed in laboratory tests
suggests a high likelihood of extirpation, and conversely very low exposure levels implies that
extirpation is unlikely. Population modeling (such as population viability analysis) or ecosystem
modeling may be required to estimate the likelihood of extirpation in cases where exposure is
lethal to only a portion of individuals, where effects on reproduction are expected but limited, or
effects are indirect. Population modeling typically requires species-specific data on parameters
not routinely available in ecological risk assessment, such as age-specific reproduction rates.

See also Section A.2.2.2.

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A.2.2. GEAE #6: Abundance of An Assessment Population

A.2.2.1. Laws, Regulations, and Precedents

Abundance is the most common population-level endpoint considered by EPA. On
occasion, EPA has used population models to assess effects on abundance by chemicals regulated
under TSCA. For example, EPA evaluated the risks of chloroparaffins to a rainbow trout
population using a projection matrix model (U.S. EPA, 1993a). Maintenance of populations of
piscivorous birds and mammals was the ecological assessment endpoint for the Mercury Report
to Congress (U.S. EPA, 1995).

Additionally, more than 25 estuaries have been selected as National Estuaries by EPA as
authorized by the CWA. Restoring or protecting populations and production of fish and shellfish
for commercial and recreational use typically are among the goals of individual National Estuary
programs. Similarly, a goal of the Chesapeake Bay Program (a partnership among EPA and the
states adjoining the Bay) is restoring, protecting, and enhancing fish and shellfish, with measures
including populations of oysters and priority migratory fish species such as striped bass.

A.2.2.2. Practicality

Changes in population abundance may be predicted using conventional toxicity data,
statistical extrapolation models, and population models. This approach can produce reasonable
results, at least in controlled conditions. For example, Kuhn et al. (2001) recently compared
mysid shrimp population prediction from a stage-based projection matrix model with a 55 day
laboratory population study involving shrimp exposed to p-nonylphenol. The population model
was able to project within a few micrograms per liter the concentration where population-level
effects would begin to occur (projected 16 |ig/L from the model vs. measured 19 |ig/L from the
assay). Population abundance may also be estimated using individual-based population models
or, as discussed in Section A.3., ecosystem models. Measurement of changes in population
abundance in the field may be easy (e.g., flowering plants) or difficult (e.g., pelagic cetaceans).

A.2.3. GEAE #7: Production of An Assessment Population

A.2.3.1. Laws, Regulations, and Precedents

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Much of the support for GEAE #6, abundance of an assessment population, also applies
to this endpoint. For example, the Clean Water Act sets a national goal of "protection and
propagation of fish, shellfish, and wildlife," which implies both abundance and production, and
efforts under the National Estuary and Chesapeake Bay Programs to protect resource species
involve both abundance and production. Additionally, numerous federal laws and treaties have
the purpose of maintaining or increasing the production of game birds and mammals, commercial
fish, and timber species. Examples include the Migratory Bird Hunting Stamp Act (48 Stat.
451), Wildlife Restoration Act (50 Stat. 917), Fish Restoration and Management Act (64 Stat.
430), Convention on Great Lakes Fisheries (6 UST 2836), and Fish and Wildlife Act of 1956 (70
Stat. 1119). Relevant provisions include requirements to "develop measures for maximum
sustainable production of fish" (70 Stat. 1119) and "make possible the maximum sustained
productivity of Great Lakes fisheries" (6 UST 2836).

Prevention of adverse effects to public welfare is mandated under Section 108 of the
CAA (National Ambient Air Quality Standards), including (but not limited to) effects on soils,
water, crops, vegetation, animals, and wildlife (§109). EPA has included production of an
assessment population, among other endpoints, as an indicator of public welfare. For example,
EPA revised the secondary ozone standard to provide increased protection against ozone-induced
effects on vegetation, such as agricultural crop loss and damage to forests (U.S. EPA, 1997b).
Also, EPA regulations authorize the Agency to require pesticide registrants to submit tests on
pesticide effects on plant mortality and plant growth inhibition. Results from these tests are used
by EPA, in conjunction with other available information, in making pesticide registration
decisions. Changes in production of specific legume species were endpoints in a TSCA
assessment of release of recombinant rhizobia (McClung and Sayer, 1994; Orr et al., 1999).

A.2.3.2. Practicality

Plant production is relatively easily and commonly measured in the field. Production of
animals is more difficult to measure in the field, but well developed techniques exist and are
commonly employed for fisheries, game species, and pest insects. Toxic effects on production
may be estimated from chronic tests that include survival, fecundity and growth. The combined
effects on population production of these organismal responses may be estimated using
population or ecosystem models.

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A.3. Community-Level Endpoints

To date, the most common application at EPA of direct assessment of community-level
endpoints has been in the context of aquatic communities, particularly fish and macroinvertebrate
communities. Section 101(a)(2) of the Clean Water Act calls for an interim goal of water quality
which provides for the protection and propagation of fish, shellfish, and wildlife. Section 304(a)
of the Water Quality Act of 1987 directs EPA to develop and publish water quality criteria and
information on methods, including biological monitoring and assessment methods, that assess the
effects of pollutants on the aquatic community. Aquatic community components and attributes
addressed include "biological community diversity" and "productivity." Species richness and
abundance of fish and macroinvertebrate communities are used in the biocriteria of many states
and in Agency guidance (Yoder and Rankin, 1995; U.S. EPA, 1996).

In addition, potential community-level impacts have been inferred and considered a basis
of concern by EPA programs based on organism-level responses. The U.S. Ambient Water
Quality Criteria for Protection of Aquatic Life are based on Species Sensitivity Distributions
(SSDs), with the criteria set at the fifth percentile (U.S. EPA, 1985). Hence, they can be
interpreted as protecting at least 95% of species in a community. The assessment community is
also commonly used in EPA programs under TSCA. The Quotient Method is typically applied to
the most sensitive organismal response, as well as uncertainty factors, to infer effects on a
community. Organisms are chosen to represent a variety of taxonomic groups.

EPA's principles for ecological risk assessment and risk management at Superfund sites
state that "Superfund's goal is to reduce ecological risks to levels that will result in the recovery
and maintenance of healthy local populations and communities of biota," with community effects
either measured directly (e.g., benthic species diversity) or estimated indirectly (e.g., from
toxicity tests on individual species) (U.S. EPA, 1999b). Ecosystem models are particularly useful
for assessing secondary (indirect) effects of toxicants on community properties (Bartell et al.,
1992). EPA has occasionally used ecosystem models to estimate community-level effects, as in
case of evaluating the primary and secondary effects of chloroparaffins to top predator fish
(Bartell, 1990; U.S. EPA, 1993a).

A.3.1. GEAE #8: Species Richness of An Assessment Community

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A.3.1.1. Laws, Regulations, and Precedents

In addition to the applications cited in Section A.3., aquatic community composition is
presented as an example of an assessment endpoint in Superfund ecological risk assessment
guidance (U.S. EPA, 1997c) and community diversity or species richness is a generic endpoint
for ecological risk assessments of hazardous waste combustors (U.S. EPA, 1999a). EPA regional
offices have considered effects of federal projects on species diversity in decisions under NEPA,
such as in an assessment of the impacts of the loss of bottomland hardwood forest on species
composition of the wildlife community due to levee construction (U.S. EPA, 1994).

A.3.1.2. Practicality

Species richness is the simplest, least controversial, and most easily interpreted
expression of community diversity. Changes in species richness are readily observed in standard
biological surveys. If it is assumed that significant toxic effects are likely to result in local
extirpation of a species, changes in species richness may be predicted using species sensitivity
distributions or regression models that relate all species of a community or assemblage to a test
species. If indirect effects are expected to result in the loss of species, ecosystem models may be
used to predict species losses.

A.3.2. GEAE #9: Abundance of An Assessment Community

A.3.2.1. Laws, Regulations, and Precedents

As described in Section A.3, this GEAE has most often been applied to aquatic
communities. Abundance of fish and macroinvertebrate communities is used in the water quality
biocriteria of many states and in Agency guidance, and community abundance can be inferred to
be an element of ambient water quality standards and of chemical evaluations under TSCA.
Aquatic community composition (including a metric describing abundance) is presented as an
example of an assessment endpoint in Superfund ecological risk assessment guidance (U.S. EPA,
1997c).

A.3.2.2. Practicality

Abundance of communities or assemblages, either as a whole or by species, are available

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from most routine biological surveys. While one can readily infer from standard toxicity tests
that some changes in abundance are likely to occur, they are difficult to predict quantitatively.
As discussed in Section A.3., community properties may be estimated from standard toxicity test
data using ecosystem models.

A.3.3. GEAE #10: Production of A Plant Assemblage

A.3.3.1. Laws, Regulations, and Precedents

This endpoint shares a basis in laws, regulations, and precedents with production of plant
populations described in GEAE #7, such as under FIFRA, TSCA, and CAA programs. For
example, the secondary ambient air quality standard established by EPA to protect public welfare
for ground-level ozone (U.S. EPA, 1997b) cited growth and yield reductions in tree seedlings and
mature trees, and impacts on forest stands and community structure due to these reductions.

Superfund directives and guidance identify plant production, such as productivity of
wetlands vegetation, as candidate assessment endpoints (Environmental Response Team, 1994a,
b, c, d). Community productivity and, in particular, herbaceous plant productivity, is a generic
endpoint for ecological risk assessments of hazardous waste combustors (U.S. EPA, 1999a). EPA
actions to control acid rain and its precursors have been based on concerns over damage to high
elevation forests, among other effects, attributed to acid rain.

As stated in Section A.3., the Clean Water Act (§101(a)(2)) calls for an interim goal of
water quality which provides for the protection and propagation of fish, shellfish, and wildlife.
Section 304(a) of the Act also lists effects of pollutants on plant life and on rates of
eutrophication as factors to consider in establishing pollutant limits. Excessive plant production
and its sequelae (e.g., fish kills from low dissolved oxygen) due to nutrient pollution
(eutrophication) has been the basis for many federal and state regulatory actions and voluntary
control programs. These include establishing Total Maximum Daily Loads (TMDLs) for
nutrients (U.S. EPA, 1999c), controls on nutrient discharges from sources such as publicly
owned treatment works (POTWs) and confined animal feeding operations, and restrictions on
phosphorus in detergents.

A.3.3.2. Practicality

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Eutrophication has long been a major concern of environmental managers, particularly
with respect to sewage outfalls, so the models for predicting effects of nutrient additions are
relatively well developed. Similarly, studies of fertilizer addition to crops, pastures and
commercial forests are numerous and provide a good basis for predicting effects of terrestrial
nutrient additions on plant production. In addition, methods for measuring plant production are
well developed for both terrestrial and aquatic communities. Protocols for testing toxic effects
on terrestrial and aquatic plants focus on various measures of production. However, toxicity data
for plants are less abundant than for animals.

A.4. Ecosystem and Location-Specific Endpoints

From an ecological perspective, attributes that characterize ecosystems include primary
production, energy flow, total biomass, and nutrient cycling. The authors of this report found
little precedent for using such endpoints at EPA (with the exception of functional properties of
wetlands), perhaps because these concepts are somewhat abstract and not as directly linked to
management values of concern as other endpoints. Nevertheless, abundant statutory and
regulatory support exists for environmental protection at levels above the organism, population,
and even community level. This is both due to the recognition that to maintain particular
organisms of concern their surrounding environment must be preserved (e.g., in the case of
endangered species) and due to appreciation for the ecosystem as a whole (e.g., National Parks,
coral reefs). Endpoints based on these concerns tend to be location-specific and defined by the
area they encompass and sometimes by particular properties they exhibit within the area. While
arguably some of the following endpoints could be regarded as community-level endpoints, they
are collected in this section on the basis of their shared association with particular geographical
locations and their broadly-defined attributes that are not restricted to a particular level of
biological organization.

A.4.1 GEAEs #11 & 12: Area and Function of Wetlands

A.4.1.1. Laws, Regulations, and Precedents

The Clean Water Act forms the primary statutory basis for this endpoint. In meeting the
Clean Water Act's objective of restoring and maintaining the integrity of the Nation's waters,

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under Section 404 of this Act wetlands are considered "waters of the United States" and are
protected from discharge of dredged and fill material through a permit program jointly
administered by the U.S. Army Corps of Engineers and EPA. Wetlands are defined for regulatory
purposes as areas that are inundated or saturated by surface or ground water at a frequency and
duration sufficient to support, and that under normal circumstances do support, a prevalence of
vegetation typically adapted for life in saturated soil conditions. Wetlands generally include
swamps, marshes, bogs, and similar areas (33 CFR §328.3(b); 1984). The Clean Water Act
provides authority for the Corps to require permit applications to avoid and minimize wetlands
impacts and requires EPA to develop, in coordination with the Corps, the criteria used for
Section 404 permit decisions. When damages to wetlands are unavoidable, the Corps can require
permitees to provide compensatory mitigation. Compensatory mitigation activities may include
enhancement, restoration, creation or preservation efforts associated with the impacted wetlands
or other wetlands preferably within the same watershed.

Additionally, Executive Order 11990, Protection of Wetlands, requires that "Each agency
shall provide leadership and shall take action to prevent the destruction, loss or degradation of
wetlands and to preserve and enhance natural and beneficial values of wetlands in carrying out
the agency's responsibilities" (Carter, 1977). As an extension of this order, President George
Bush in 1989 and succeeding Presidents have adopted a national policy of no net loss of wetlands
in recognition of the significance of wetland areas and their ecological functions. The 1972
Coastal Zone Management Act also calls for the protection of coastal wetlands.

EPA has prepared various regulations and guidance documents supporting the wetlands
protection goals of the CWA and Executive Order. For example, the Guidelines for Specification
of Disposal Sites for Dredged or Fill Material (40 CFR Part 230, Subpart E) require
consideration of potential impacts on special aquatic sites, including wetlands, referencing
changes that result in loss of wetland status due to permanent flooding or conversion to dry land
as well as loss of functions of water purification, water storage, and provision of wetland habitat.

Due to the large number of Superfund sites in or adjacent to wetlands, EPA's policy and
emphasis has lead to a greater concern regarding the impact of contamination from Superfund
sites on the extent and ecological functions of wetlands. EPA's Office of Solid Waste and
Emergency Response (OSWER) highlights the importance of wetlands protection in their
directive, "Policy on Floodplain and Wetland Assessment for CERCLA Action" (U.S. EPA,

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1985). Under this policy, Superfund action must meet the substantive requirements of the
Floodplain Management Executive Order (E.O. 11988) and the Protection of Wetlands Executive
Order 11990.

Throughout the Superfund process, site managers should address the potential ecological
impacts from response actions to both on-site and adjacent wetland resources. Prior to the
national listing of a site, information regarding the presence of wetlands is factored into the
Hazard Ranking System (U.S. EPA, 1990a and 1992b). During any removal actions, the
Superfund Removal Process Guidance (U.S. EPA, 1992a) requires the On-Scene Coordinator to
undertake special considerations for actions that include wetlands. From the initiation of the on-
site investigation during the Remedial Investigation/Feasibility Study to evaluating the
effectiveness of remedial alternatives, requirements set forth in E.O. 11990 and Section 404 of
the Clean Water Act should be considered and any unavoidable impacts to wetlands must be
mitigated (U.S. EPA, 1984). Section 404 of the Clean Water Act is also considered a federal
"applicable or relevant and appropriate requirement" (ARAR) with which Superfund remedial
actions must comply under CERCLA Section 121(d)(2)(A). Types and levels of mitigation
necessary to demonstrate compliance with Section 404 are discussed in a Memorandum of
Agreement (MOA) between the U.S. EPA and the Department of Army (U.S. EPA, 1990b). In
general, the MOA indicates that any enhancement, restoration, creation, or replacement of
wetlands should be based on functional equivalence to include the minimum of a one to one
ratio. The prevention of secondary wetland impacts due to activities in or adjacent to a wetland
as part of the Superfund response action are addressed in the OSWER Directive, Controlling the
Impacts of Remediation Activities In or Around Wetlands (U.S. EPA, 1993c).

EPA's "Procedures for Implementing the Requirements of the Council on Environmental
Quality on the National Environmental Policy Act" (40 CFR §6.108) singles out wetlands in
stating that "if the proposed action may have significant adverse effects on wetlands" an
Environmental Impact Statement is required. EPA's regulations for State and Local Assistance
(40 CFR Part 35, Appendix A to Subpart H) require that project proposals demonstrate
compliance with Executive Order 11990.

The Wetlands Loans Act (75 Stat. 813) established financing for acquisition and
restoration of wetlands. While this Act places no obligations on EPA, it further demonstrates
Congressional intent to protect and enhance wetlands.

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Ecological risk assessment case studies sponsored by EPA have used attributes of
wetlands as assessment endpoints. A case study of physical disturbance of wetlands used water
purification with respect to specific pollutants as the assessment endpoints (Detenbeck, 1994).

A.4.1.2. Practicality

Wetlands are classified and mapped by the National Wetlands Inventory of the U.S. Fish
and Wildlife Service, but determination of wetland boundaries at a given site may be difficult,
particularly in areas of low topographic relief. The 1987 Corps of Engineers Wetlands
Delineation Manual (Environmental Laboratory, 1987) is the current federal delineation manual
used in the Clean Water Act Section 404 regulatory program for the identification and
delineation of wetlands. Most effects on wetland area are readily predicted or observed, because
they occur due to processes such as dredging, filling, draining, or inundation. Losses of wetland
functions are less readily observed or predicted except when they result from the loss of wetland
area. The Corps and Natural Resources Conservation Services (NRSC) have agreed to formally
adopt the Hydrogeomorphic Method (Brinson, 1993) for assessing wetland function under the
Clean Water Act Section 404 Program. Toxic effects on wetland functions or on the type of
wetland community are difficult to predict.

Within the Superfund program, unavoidable impacts to on-site and adjacent wetland
resources from current or potential exposure to hazardous substances and from implementation
of selected response actions are addressed within the Record of Decision for that site. Records of
Decision for the New London Submarine base in New London, Connecticut (U.S. EPA, 1998c),
Loring Air Force Base in Limestone, Maine (U.S. EPA, 1997d) and Pease Air Force Base in
Portsmouth/Newington, New Hampshire (U.S. EPA, 1997e) include remedies involving
compensatory wetland mitigation. Mitigation actions are monitored through the development of
long-term monitoring plans and an annual and five year review process to ensure the success of
the wetlands restoration efforts.

A.4.2. GEAEs #13 & 14: Area and Species Richness of Coral Reefs
A.4.2.1. Laws, Regulations, and Precedents

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At present, coral reefs have not attained the same legal and regulatory stature under EPA
programs as wetlands, but the basis for their protection has been increasing. Executive Order
13089 on Coral Reef Protection establishes special protection for coral reefs (Clinton, 1998). In
particular, "All Federal agencies... shall.. .utilize their programs and authorities to protect and
enhance the conditions of such ecosystems." This Executive Order names the EPA Administrator
as a member of the Coral Reef Task Force, which is responsible for implementing the Order. An
EPA memorandum to the field specifically applies EO 13089 to EPA's responsibilities under
Section 404 of the Clean Water Act, Section 102 and 103 of the Marine Protection and
Sanctuaries Act, and Section 307 of the Coastal Zone Management Act (Fox and Westphal,
1999). The order is also considered a federal "applicable or relevant and appropriate
requirement" (ARAR) with which Superfund remedial actions must comply under CERCLA
Section 121(d)(2)(A). The Guidelines for Specification of Disposal Sites for Dredged or Fill
Material (40 CFR Part 230, Subpart E) require consideration of potential impacts on special
aquatic sites, including coral reefs. The Guidelines refer to loss of productive colonies and
subsequent loss of coral-dependent species.

Diversity is the only ecological attribute defined as a value of coral reefs in the National
Action Plan to Conserve Coral Reefs (U.S. Coral Reef Task Force, 2000). A practical
operational definition of that attribute is species richness. This document also mentions
"shoreline protection, areas of natural beauty, recreation and tourism, and sources of food,
pharmaceuticals, jobs, and revenues" as services of coral reefs. These services could be protected
by preserving the area and species richness of coral reefs.

The Convention on International Trade in Endangered Species (CITES), to which the
U.S. is a party, restricts international trade in corals and other reef organisms. All coral reefs in
Florida are protected by either the U.S. or State government. Other specifically protected reef
communities are found in Puerto Rico, Hawaii, the U.S. Virgin Islands, Guam, Northern
Marianas, American Samoa, and several small outlying islands.

Despite the regulatory and other policy support for protecting coral reefs, there is little
precedent for use of this endpoint at EPA to date, perhaps because EO 13089 is new and few
EPA actions involve coral reefs.

A.4.2.2. Practicality

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The area of coral reef and the species richness of corals are relatively easily determined.
The species richness of some other assemblages (e.g., fishes and sessile noncoral invertebrates) is
practical to determine. Methods for assessing the condition of coral reefs are discussed by
Jameson et al. (1998). Prediction of effects of pollutants on coral reefs is difficult due to the
paucity of toxicological information for corals.

A.4.3. GEAEs #15 & 16: Area and Quality of Habitat for Threatened or Endangered
Species

A.4.3.1. Laws, Regulations, and Precedents

The obligation to protect endangered and threatened species under the ESA includes
protection of the critical habitats on which they depend. Thus the legal and regulatory basis for
protecting endangered species described under GEAE #3 generally also applies to this endpoint.
For example, the Superfund NCP specifies that, "evaluations shall be performed to assess threats
to the environment, especially sensitive habitats and critical habitats of species protected under
the Endangered Species Act" (emphasis added) (U.S. EPA, 1989). EPA's regulations for "State
and Local Assistance" (40 CFR Part 35, Appendix A to Subpart H) require that project proposals
determine whether there would be significant adverse effects on critical habitat of endangered
species.

A.4.3.2. Practicality

Designated critical habitat is readily identified, and it should be practical to determine
whether it will be destroyed (reduced area) or adversely modified (reduced quality). However,
critical habitat has not been officially designated for many endangered or threatened species.
Critical habitat that has not been officially designated must be identified based on the distribution
of the species and its ecological requirements. Toxic effects may be predicted if species or taxa
that are components of critical habitat are identified and their response to pollutants can be
evaluated.

A.4.4. GEAE #17: Area of an Endangered or Rare Ecosystem Type

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A.4.4.1. Laws, Regulations, and Precedents

Support for this endpoint at EPA is less extensive and more indirect than for many of the
other GEAEs, but can be identified in a variety of programs. In particular, several lines of support
are apparent in Superfund programs. The NCP specifies that, "evaluations shall be performed to
assess threats to the environment, especially sensitive habitats" (emphasis added) (U.S. EPA,
1989). The Hazard Ranking System for Superfund (U.S. EPA, 1990a) gives as an example of
'sensitive environments': 'particular areas, relatively small in size, important to maintenance of
unique biotic communities.' The Superfund Removal Process Guidance (U.S. EPA, 1992a)
requires the On-Scene Coordinator to undertake special considerations for actions that include
sensitive ecosystems, which may be interpreted as calling for protection of endangered or rare
ecosystem types.

Other EPA programs also consider endangered ecosystems. For example, the protocol for
screening-level ecological risk assessment for hazardous waste combustion facilities calls for
special consideration of areas having unique and/or rare ecological receptors and natural
resources (U.S. EPA, 1999a). EPA Regions 4, 5, 6 and the Great Lakes Program Office are
developing approaches for identifying high quality area (critical ecosystems) for enhanced
environmental protection and restoration. EPA Region 4 has been involved in the development
of the Southeastern Ecological Framework (SEF) as a decision support tool useful in integrating
program resources for protecting and sustaining ecological processes. EPA Region 5 also
developing an approach for prioritizing and targeting high-quality areas in the Midwest (Mysz et
al., 2000). Two of the criteria for identifying these areas, also called "critical ecosystems," share
features with this GEAE: 1) presence of an indigenous ecosystem and biological community
types (used as an indicator of relative ecological diversity), and 2) numbers and rarity of native
species and natural features (used as indicators of surviving relict native ecosystems). In addition,
the EPA Great Lakes program in collaboration with Environment Canada have developed
Biodiversity Investment Areas (BIAs) as natural areas along the Great Lakes shoreline with high
ecological value that warrant exceptional attention to protect them from degradation. EPA
Region 6 is using a GIS screening tool to assist in prioritizing ecological areas of concern for
programs like NEPA (Osowski et al., 2001).

In carrying out its responsibilities for reviewing Environmental Impact Statements under

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NEPA, EPA has developed guidance that calls for paying special attention to human activities in
imperiled ecosystems and identifies mitigation measures to reduce adverse impacts (U.S. EPA,
1993b). Approximately a dozen "principal habitats of concern" were identified within each of
six major U.S. habitat types. Ecological concerns raised by EPA to other federal agencies in
review of NEPA documents have included impacts to endangered or rare ecosystems (U.S. EPA,
1994).

State Natural Heritage programs identify rare ecosystem types and their locations and,
depending on the state, provide for protection or special consideration of those areas.

A.4.4.2. Practicality

An endangered or rare ecosystem type might be diminished by physical destruction,
which is readily observed and quantified, or by physical conversion to another type (e.g., due to
selective logging or grazing), which can also be readily observed and quantified if the type is
clearly defined. The prediction of loss of an ecosystem type due to extirpation of many or most
of the constituent organisms (e.g., due to an herbicide application or oil spill) is practical since it
would involve severe toxicity. However, loss of a type due to more subtle effects, such as
changes in species composition due to differential susceptibility to a stressor, could be difficult to
predict. Information useful in identifying rare and endangered ecosystem types is available from
NatureServe (www.natureserve.org), a nonprofit organization that works with natural heritage
programs throughout the United States and elsewhere in the Western Hemisphere. NatureServe
maintains databases on all known ecological communities in the United States, ranked from
critically imperiled to secure. According to NatureServe, the completeness of inventory and
classification work varies widely among states, provinces and regions.

A.4.5. GEAE #18: Physical Structure of an Aquatic Ecosystem

A.4.5.1. Laws, Regulations, and Precedents

The Clean Water Act [§101(a)] states that, " The objective of this Act is to restore and
maintain the chemical, physical, and biological integrity of the Nation's waters" (emphasis
added). The importance of physical structure is reflected by EPA regulations implementing the
CWA that note the following conditions of a water body that may preclude attainment of desired

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beneficial uses (40 CFR §131.10 (g)):

"natural, ephemeral, intermittent or low flow conditions of water levels"

"dams, diversions or other types of hydrologic modifications"

"physical conditions related to the natural features of the water body, such as the lack of a
proper substrate, cover, flow, depth, pools, riffles, and the like, unrelated to water
quality"

The Protocol for Developing Sediment TMDLs lists channel modification, pool filling,
filling of substrate with fine sediments and other effects on physical structure as sediment issues
that can result in loss of designated uses (U.S. EPA, 1999d). These changes in stream
ecosystems are themselves changes in the ecosystem attributes that result in the lost
recreational/aesthetic or other uses, and not simply stressors that affect biological endpoints.

Physical structure has been a factor used in setting the designated use of streams in state
water quality standards. For example, in Ohio, a designated use of Modified Warmwater Habitat
applies to streams with extensive and irretrievable physical habitat modifications.

A.4.5.2. Practicality

Physical characteristics often are readily observed or measured at sites being assessed and
are usually recorded in biological surveys. Protocols exist for measuring many aquatic habitat
attributes. In addition, most of the actions that modify the physical structure of water bodies
(e.g., channelization, dam construction and operation, water withdrawals, and culvert
installation), have obvious effects on structure which are readily predicted. Other effects, such as
changes in hydrology resulting from changes in land use, are more difficult, but still possible, to
model.

A.4.6. GEAE #19: Ecological Properties of Special Places

A.4.6.1. Laws, Regulations, and Precedents

The legislative acts establishing the National Parks and Monuments, Wildlife Refuges,
Wilderness Areas, Wild and Scenic Rivers, Recreation Areas, Marine Sanctuaries, and other
special places establish their status and indicate the properties for which they were provided with

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protected status. Several statutes either give EPA a role in designating special places or direct
EPA to consider environmental impacts to such places in administering Agency programs. The
CWA directs EPA to administer the National Estuary Program, and permits states to designate
waterbodies as Outstanding National Resource Waters, which then receive increased protection
in their water quality standards. The Clean Air Act also has several provisions for special places.
Section 160 of the CAA establishes that a purpose of the Act is "to preserve protect, and enhance
the air quality in national parks, national wilderness areas, national monuments, national
seashores, and other areas of special national or regional natural, recreational, scenic, or historic
value." Section 162 designates national (and international) parks, wilderness areas, and memorial
parks of a certain size as "class I" areas that merit the highest level of protection from air
pollution. Other special places cited in both the CAA and CWA include the Great Lakes,
Chesapeake Bay, and Lake Champlain.

In the area of EPA regulations and guidance, the NCP cites special places such as
National Marine Sanctuaries and Estuarine Research Reserves as natural resources to be
protected under CERCLA. The Superfund Removal Process Guidance (U.S. EPA, 1992a)
requires the On-Scene Coordinator to undertake special considerations for actions that include
wild and scenic rivers. EPA procedures for implementing NEPA (40 CFR §6.108) require an
environmental impact statement to be prepared if "the proposed action may have significant
adverse effects on parklands, preserves, or areas of recognized scenic, recreational, archeological,
or historic value." The Guidelines for Specification of Disposal Sites for Dredged or Fill Material
(40 CFR, Part 230, Subpart E) requires consideration of potential impacts on special aquatic
sites, including sanctuaries and refuges. The protocol for screening-level ecological risk
assessment for hazardous waste combustion facilities calls for special consideration of areas
having legislatively-conferred protection (U.S. EPA, 1999a).

A.4.6.2. Practicality

Special places and their important ecological properties usually can be defined readily.
Given the diverse set ecological of properties at different places, it is not possible to make overall
statements about the practicality of this endpoint. Potentially, all of the surveying, testing, and
modeling methods discussed in prior sections could be applicable.

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APPENDIX B. TYPES OF VALUES ASSOCIATED WITH ASSESSMENT ENDPOINTS

EPA's Ecological Risk Assessment Guidelines define an assessment endpoint as "an
explicit expression of the environmental value that is to be protected, operationally defined by an
ecological entity and its attributes" [emphasis added] (U.S. EPA, 1998a). In the context of the
Guidelines, an environmental value refers to a component of the environment (or an ecological
entity) that society values, with some examples being endangered species, and commercially or
recreationally important species. Literature on environmental valuation covers a wide range of
ecological systems and components; for example, bays (Kahn, 1985), wetlands (Barbier, 1993),
riparian corridors (Lant and Tobin, 1989), deserts (Richer, 1995), recreation areas (Adamowicz et
al., 1994), and wilderness or "un-spoiled" natural areas (Hanink, 1995; Kopp and Smith, 1993;
and Randall and Peterson, 1984). In many of these studies, ecosystems are conceptualized as
having assets or structural components such as energy resources, minerals or timber; services or
natural functions benefitting society (e.g., groundwater recharge, flood control, or the absorption
or assimilation of pollutants) and/or other attributes provided by the whole ecosystem such as
biological diversity, cultural uniqueness, or natural heritage (Westman, 1977; Daily et al., 1997).

Table B.l presents one way of organizing environmental values, drawing on Blomquist
and Whitehead (1995), Daily (2000), Ehrlich and Ehrlich (1981), MacLean (1995), Primack
(1993) and Freeman (1984, 1993). Table B.l is not intended to represent a definitive or
comprehensive list of environmental values, but rather is intended to illustrate the breadth of
values that may be cited in support of a GEAE. Another common approach in the literature is to
distinguish between use values (e.g., natural resources consumed or used in economic markets)
and non-use values (ecological attributes with social values beyond mere economic
considerations). In this context, "consumptive" and "information" values in Table B.l could
typically be considered as use values, and "educational" and "preservation" values as non-use
values. "Functional," "option" and "recreational" values in Table B.l could be considered as use
or non-use values depending on the context in which they are used. Non-use values are also
sometimes characterized as "existence," "intrinsic," "preservation," or "passive use" values.
Nevertheless, when quantified in cost-benefit analyses, non-use values may, in some cases, be
more significant than use values (Ehrenfield, 1976; Randall, 1984; Smith, 1993; and Kahn,

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1	1995).

2	Each of the GEAEs presented in this document relate to one or more of these

3	environmental values. For example, an "assessment population" and its attributes may be used to

4	represent a commercially and recreationally valuable fish or wildlife population (consumptive

5	and recreational values). Such an assessment population could also represent a species

6	population that is valued as a learning tool (educational value) and protected for cultural and

7	aesthetic reasons (preservation value). Table B.l provides further examples of how each of the

8	GEAEs may correspond with these values.

9

10	Table B.l. Some Categories of Environmental Values.

11

12

Value

Definition and examples

13

14

Consumptive
value

Definition: the value of commodities produced by the environment such as food,
energy, timber, fiber, and pharmaceutical and industrial products.





Examples of corresponding GEAEs:

•	Assessment population and coral reefs: commercially valuable fisheries

•	Plant production: timber and fuel production by trees

•	Organisms (in an assessment population): commercially valuable fiirbearers

15

16

Information
value

Definition: the value of natural structures, chemicals or processes as models for
anthropogenic structures, chemicals or processes (e.g., pharmaceuticals, synthetic
commodities and engineering designs). Also see Option value.





Examples of corresponding GEAEs:

•	Endangered/rare ecosystem types: as examples of process integration

•	Species richness of communities: as sources of bioactive chemicals as models
for pharmaceuticals

•	Organisms (threatened and endangered species): as sources of model
adaptations to extreme environments

17

18

Functional
value

Definition: the value of ecological functions benefitting public health and welfare,
such as pollen and seed dispersal, water retention and purification, detoxification
of wastes, and moderation of weather extremes. In some cases, ecosystems are re-
established to make use of their functional value for remediation.





Examples of corresponding GEAEs:

•	Wetlands: water retention and purification

•	Assessment community or assemblage: water and soil retention by forests

•	Assessment population: pollination by insects

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Value

Definition and examples

1

2

Recreational
value

Definition: the value of recreational opportunities such as fishing, birding, boating,
and hiking. In some cases, this is a passive use of a resource, but in others (e.g.,
tourism) it is an economic activity.





Examples of corresponding GEAEs:

•	Wetlands, coral reefs, and aquatic ecosystems: boating, fishing

•	Organisms (migratory birds): birding, hunting

•	Special places: camping, hiking, boating

3

4

Educational
value

Definition: the value of academic and nonacademic educational opportunities,
including nature and scientific study.





Examples of corresponding GEAEs:

•	Special places: parks and wildlife refuges for nature study and research

•	Wetlands and aquatic ecosystems: environmental education sites

5

6

Option value

Definition: the value to future generations of preserving the option of using the
environment at some future time. Option value also includes human welfare gains
or net benefits associated with delaying a decision when there is uncertainty about
the payoffs of certain alternatives, or when one of the choices involves an
irreversible commitment of resources.





Examples of corresponding GEAEs:

•	Wetlands and aquatic ecosystems

•	Special places

•	Endangered/rare ecosystem types

7

8

Existence
value

Definition: value ascribed to the existence of ecological systems independent of
any direct services or functions. Aesthetic, moral, cultural, religious, or spiritual
grounds may be cited in support of this type of non-use value.





Examples of corresponding GEAEs:

•	Organisms-endangered species and their critical habitat

•	Organisms-marine mammals

•	Endangered/rare ecosystem types

•	Special places

9
10

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