Data Report Characterization of Bryan Mound Crude Oil for the National Oil and Hazardous Substances Pollution Contingency Plan Product Schedule (NCPPS)


Data Report:

Characterization of Bryan Mound Crude Oil for the National Oil and Hazardous Substances
Pollution Contingency Plan Product Schedule (NCPPS)

PREPARED BY:

Robyn Conmy

U.S. Environmental Protection Agency
Office of Research and Development
Cincinnati, OH 45268

Devi Sundaravadivelu and Raghu Venkatapathy
Pegasus Technical Services, Inc.

In-house Contractor to U.S. EPA ORD
Cincinnati, OH 45268

SUBMITTED TO:

U.S. Environmental Protection Agency
Office of Land and Emergency Management
Office of Emergency Management
Washington, DC 20004

DATE: December 7, 2023

BRIEF

This data report summarizes the physical-chemical properties, aquatic toxicity, and dispersant
effectiveness of Bryan Mound Crude (BMC) oil. BMC has been selected as a new reference oil for
the National Oil and Hazardous Substances Pollution Contingency Plan Product Schedule
(NCPPS). Analyses were conducted at the US Environmental Protection Agency (US EPA) Office
of Research and Development (ORD) AWBERC Facility in Cincinnati, OH, and two contracted labs,
Core Laboratories Saybolt in Deer Park, TX and Hydrosphere Research in Alachua, FL. ORD
coordinated with the Office of Land and Emergency Management (OLEM) Office of Emergency
Management (OEM) throughout the duration of this effort.

EPA/600/X-23/356

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RELEVANCE

Under section 311 of the Clean Water Act (CWA), as amended by section 4201 of the Oil Pollution
Action of 1990 (OPA), the President is directed to prepare and publish the National Oil and
Hazardous Substances Pollution Contingency Plan (NCP) for removal of oil and hazardous
substances. Section 311(d)(2)(G) directs the President to include a Schedule identifying
dispersants, other chemicals, and other spill mitigating devices and substances, if any, that may
be used in carrying out the NCP. The authority of the President to implement this portion of the
CWA is currently delegated to the U.S. Environmental Protection Agency (EPA) (56 FR 54757,
1991). Subpart J of the NCP governs the use of chemical or biological agents to respond to oil
discharges.

The predecessor of the NCP was first published in 1970 (35 FR 8508) and contained Annex X -
Schedule of Dispersants and other Chemicals to Treat Oil Spills. EPA became responsible for
Annex X in 1973 (38 FR 21243). In 1994, EPA revised the NCP in response to the passage of the
OPA (59 FR 47384) to its current regulatory requirements with respect to the authorization of
use, data requirements for listing, and effectiveness and toxicity testing protocols for products
on the Schedule. The current NCPPS Technical Notebook is maintained by EPA and contains a
compilation of manufacturer product bulletins provided on safety, storage, application methods,
toxicity and effectiveness data, and physical properties.

Oil spill remediation countermeasure products must be evaluated and approved before they may
be used to remove or control discharges. Products listed in the NCP Product Schedule can be
found at https://www.epa.gov/emergency-response/alphabetical-list-ncp-product-schedule-
products-available-use-during-oil-spill. EPA requires that product manufacturers submit toxicity
data for all products listed on the NCPPS. Dispersants and bioremediation agents must also
undergo effectiveness testing using reference oils in accordance with the published testing
protocols developed by the EPA (Venosa, 2002; Haines et al., 2003; Sorial et al., 2004 a and b).

NCPPS reference oils are maintained by the EPA. Bryan Mound Crude (BMC) oil was selected as
a new reference oil for dispersant effectiveness testing (88 FR 38333, effective December 11,
2023) to replace dwindling supplies of existing oil. To be listed on the NCPPS, the dispersant must
demonstrate for each temperature a dispersant effectiveness at the 95% lower confidence level
(LCL95) greater than or equal to 75% at 25°C and 70% at 5°C. Reported here are the results of
BMC testing for chemical and physical characterization, dispersant effectiveness using select
NCPPS dispersant products, and acute toxicity of BMC to two standard test species: an estuarine
crustacean (mysid; Americamysis bahia) and an estuarine fish (inland silverside; Menidia
berylina). Results will be submitted to OLEM in support of the NCPPS and decision-making by On-
Scene Coordinators (OSCs) regarding products for use during emergency response operations.

EPA/600/X-23/356

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METHODOLOGY

Quality Assurance Project Plans
L14866-QP-1-7 Category A
G-LRPCD-0021545-QP-l-l Category B

Oil and Dispersants

Bryan Mound Crude (BMC) is a light, sweet crude oil, obtained from the Department of Energy
Strategic Petroleum Reserve in 2021. Eight chemical dispersant products currently listed on the
NCPPS were used to treat the oil and evaluate the dispersant effectiveness. Chemical dispersant
products used were (in alphabetical order) Accelf Clean DWD, Corexit® EC9500A, Dispersit SPC
1000™, Finasof OSR 52, JD-2000™, Nokomis 3-AA, Saf-Ron Gold, and ZI-400. Products were
chosen based on availability at the time of testing. Results are reported 'masked' to avoid bias.

Dispersant Effectiveness Baffle Flask Test

The EPA Baffle Flask Test (BFT) procedure was used for determining Dispersant Effectiveness (DE)
for a specific oil-dispersant-temperature combination in six 150 mL baffled trypsinizing flasks
(Venosa et al., 2002). Tests were conducted in controlled temperature rooms at 5 and 25 °C.
Artificial seawater (120-mL of 31%o adapted from Spotte et al., 1984) and 100 |aL crude oil were
added to the flask followed by 4 |aL of a dispersant pipetted directly onto the oil slick to yield a
Dispersant to Oil Ratio (DOR) of 1:25. The flasks received turbulent mixing at 250 rpm on an
orbital shaker table. Following the mixing, the contents were allowed to settle for 10 ± 0.25
minutes to allow undispersed oil to reform a slick on the seawater surface before draining 30-mL
through a stopcock at the base of the flask. The dispersed oil sample underwent liquid-liquid
extraction using dichloromethane and analyzed with a UV-vis spectrophotometer between
wavelengths of 340 - 400nm. The DE value, which is the lower 95% confidence limit of the six
independent replicates (DE LCL95) was reported for each treatment.

Physico-Chemical Characterization of Source Oil

BMC oil was shipped to Deer Park, TX for characterization by a certified laboratory. A chemical
assay for the oil was generated using standard methods and provided to the EPA. Assay methods
and results can be found in the attachment within the Appendix. BMC oil was analyzed for
monoaromatic hydrocarbons (i.e., benzene, toluene, ethylbenzene and xylenes; BTEX), polycyclic
aromatic hydrocarbons (PAHs), and alkanes in the EPA laboratory in Cincinnati, OH. Analysis for
BTEX was performed by adding oil-soaked absorbent pads to a vial and spiking with a deuterated
BTEX mix, surrogate mix and internal standards. The samples were then quantified using an
Agilent 7890A Gas Chromatograph (GC) with a 5975C mass selective detector (MSD) with Triple
Axis Detector and CombiPal autosampler (CTC Analytics) following EPA Method 524.3 modified
to perform head space analysis instead of purge and trap (US EPA, 2009). The concentrations of
the target compounds were corrected based on the recoveries of the deuterated compounds and
reported in nanograms. The mass of oil (in mg) attached to the absorbent pads was evaluated by

EPA/600/X-23/356

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extracting with DCM and measuring on a Shimadzu UV 1800 spectrophotometer. Final results are
reported as ng BTEX per mg crude oil.

For analysis of PAHs and alkanes, oil samples were diluted in DCM and quantified using an Agilent
7890A GC with an Agilent 7000 GC/MS (GC/Mass Spectrometry) Triple Quad and a CombiPal
autosampler (CTC Analytics), equipped with a DB-5 capillary column by J&W Scientific (30 m, 0.25
mm I.D., and 0.25 mm film thickness) and a pulsed splitless injection port (US EPA, 2018). Alkanes
analyzed included C10-C35 normal aliphatics and branched alkanes (pristine and phytane). PAHs
analyzed included 2-4 ring compounds and their alkylated homologs (i.e., C0-C4 naphthalenes,
C0-C4 phenanthrenes, C0-C3 fluorenes, C0-C4 dibenzothiophenes, C0-C4
napthobenzothiophenes, C0-C4 pyrenes and C0-C4 chrysenes). Concentrations of the detected
alkanes and PAHs were summed to compute total alkane and PAH concentrations, respectively
(lag analyte/mg crude oil).

Toxicity

Toxicity testing was conducted at Hydrosphere Research, Inc. in Alachua, FL. Water
Accommodated Fractions (WAFs) were prepared with oil loadings of 25 g oil per liter of water,
under slow-stir conditions maintaining a 20% vortex for 18 h and settling for 6 h before sampling
the aqueous exposure test solutions via slow siphon. Stock solutions were then serially diluted
and used in toxicity tests (Barron and Kawaihae, 2003). Fresh source oils, stock solutions and
exposure media were extracted and analyzed for alkanes, BTEX, aromatics and Total Petroleum
Hydrocarbons (TPH) at the EPA laboratory in Cincinnati, OH. Samples were analyzed for C9-C32
TPH by gas chromatography-flame ionization detection (GC-FID) following EPA SW-846, Method
8015B-DRO. Reported concentration of stock solutions in milligrams TPH/L was used to calculate
exposure concentrations for all toxicity tests.

Acute toxicity experiments were conducted using two standard test species, estuarine crustacean
(mysid; Americamysis bahia) and an estuarine fish (inland silverside; Menidia berylina) at
Hydrosphere Research, Inc. in Alachua, FL. Toxicity data were generated from tests with five
concentrations and one control for each oil and test species. Standard toxicity test protocols and
exposure conditions followed those summarized in Barron et al. (2018). The acute toxicity of oil
was estimated using test-specific dose response data. These were generated from the average
response across replicates as percent WAF and hydrocarbon metric (BTEX, TPH, alkanes, PAH)
measured in each WAF. Effect concentrations for each metric of hydrocarbon exposure were
based on concentrations in the stock WAF solution (initial measured), or on the geometric mean
of the concentration in the stock WAF solution and exposure media at the end of the test period
(day 2 or 4 of test). All statistical analyses were performed using the R statistical platform (v.
3.3.3) and associated packages (R Development Core Team, 2018; Ritz et al., 2015). Acute
endpoints included the 20th and median lethal concentrations (LC20 and LC50, respectively).

EPA/600/X-23/356

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RESULTS

Physico-Chemical Characterization

A chemical assay for the oil was generated using standard methods and provided to the EPA.
Assay data results can be found in the attachment within the Appendix. For additional chemical
information, Department of Energy also maintains assay lists for all Strategic Petroleum Reserve
oils (https://www.spr.doe.gov/reports/Crude Oil Assays.html). BMC oil is considered a light
crude oil based on the density (0.8320 mg/ml at 15°C), API gravity (38.6 at 15°C) and viscosity
(4.721 cSt at 25°C) values. BMC is considered a sweet crude due to low sulfur content of 0.377 %
by mass. Hydrocarbon analyses showed total alkane concentration of 90.4 |-ig/mg and total PAH
concentration of 15.70 |ag/mg, on par with other light sweet crude oils.

Dispersant Effectiveness

BFT average effectiveness and DElcl95 values for BMC oil tested with masked chemical dispersants
(DOR 1:25) are presented in Table 1. Histograms of these data are presented in Figure 1. Oil
without dispersant (control) exhibited the lowest BFT DE LCL95 with values of 11.47% and 6.78 %
at 25 and 5°C, respectively. Treatments with chemical dispersant exhibited higher DE values
compared to the control. DE LCL95 values for oil treated with dispersants ranged between 51.46
- 96.10 % at 25°C and 50.52 - 91.80 % at 5°C. At 25 °C, Products B and C exhibited higher DE
compared to products D, E, and G. Temperature did not appear to substantially impact DE in
Products A, F and H.

Table 1. Dispersant Effectiveness of BMC oil treated with chemical dispersants (masked letters
A-H) and untreated control at DOR 1:25. DE average and LCL95 % values are provided.
Treatments were conducted at 25 and 5oC.

Dispersant
Masked ID

25°C

5°C

Average
(%)

Stdev (ct)

Variance
(ct2)

Coef. of
Variation
(RSD)

LCL95 (%)

Average
(%)

Stdev (ct)

Variance
(ct2)

Coef. of
Variation
(RSD)

LCLg5 (%)

A

98.44

5.92

35.10

6.02

93.57

95.13

3.49

12.18

3.67

91.80

B

98.46

2.87

8.21

2.91

96.10

93.01

6.14

37.74

6.61

87.96

C

96.98

1.51

2.27

1.55

95.54

89.16

6.28

39.49

7.05

83.99

D

89.48

5.13

26.32

5.73

85.26

90.59

1.67

2.79

1.84

89.00

E

87.74

2.68

7.20

3.06

85.18

91.56

2.52

6.34

2.75

89.16

F

58.78

8.89

79.02

15.12

51.46

55.93

6.57

43.14

11.74

50.52

G

64.03

10.22

104.49

15.96

55.62

74.86

9.10

82.88

12.16

67.37

H

76.03

6.34

40.22

8.34

70.82

78.46

11.07

122.55

14.11

69.36

Control

15.88

5.36

28.76

33.77

11.47

7.89

1.34

1.80

17.02

6.78

EPA/600/X-23/356

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¦ 25C

"5C

WML

ABCDEFGH Control

Figure 1. Dispersant Effectiveness of BMC oil treated with chemical dispersants (masked letters
A-H) and untreated control at DOR 1:25. DE average and LCL95 % values are provided.
Treatments were conducted at 25 and 5°C. The error bars represent one standard deviation
from the mean.

Toxicity

Toxicity results and monitoring of test conditions at Hydrosphere Research, Inc. laboratories can
be found in the attachment within the Appendix. Acute toxicity for BMC oil without dispersant
(oil-only) was compared using standard WAF mixing procedures. Acute definitive bioassay tests
were conducted using two standard test species: mysid shrimp (Americamysis bahia; 48-hour)
and inland silverside minnow (Menidia beryllina; 96-hour). Percent mean survival is provided in
Table 2 where the 100 % WAF resulted in a mean survival of 60 % for (A. bahia) and 67% for (M.
beryllina).

Table 2. Percent mean survival for acute toxicity testing of Bryan Mound Crude oil using
standard species Americamysis bahia (48-hour) and Menidia beryllina (96-hour).

BMC %WAF

48-hour A bahia Survival

96-hour

4. beryllina Survival



Ohr

24 hr

48 hr

Ohr

24 hr

48 hr

72 hr

96 hr

Control

100

100

100

100

100

100

100

100

6.25

100

100

100

100

100

100

100

100

12.5

100

100

100

100

100

100

100

100

25

100

100

100

100

100

97

97

97

50

100

100

100

100

100

100

100

100

100

100

60

60

100

70

67

67

67

LCso (%)

-

-

>100%

-

-

-

-

>100%

EPA/600/X-23/356

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Total petroleum hydrocarbons (TPH) concentrations in the fractional and 100% WAFs ranged
between 0.55 - 8.74 mg/L (Table 3). No reportable median lethal concentration (LC50) could be
calculated based on survival and mean concentrations, however extrapolated values (LC50*)
were calculated as 9.18 and 9.4 mg/L for A. bahia and M. beryllina, respectively. The 20th
percentile lethal concentrations (LC20) were calculated as 7.1 mg/L for A. bahia and 8.1 mg/L for
M. beryllina. Figure 2 shows the percent survival as a function of TPH for both species. EPA's new
decision-rule (88 FR 38333) does not require manufacturers to provide oil-only test results. Thus,
median LC20 and LC50 values for the BMC oil without dispersant reported here will provide a
comparative value for the EPA.

Table 3. Total Petroleum Hydrocarbon concentration within fractional WAF treatments and the
median and 20th percentile lethal concentration for acute toxicity testing of Bryan Mound Crude
oil using standard species Americamysis bahia (48-hour) and Menidia beryllina (96-hour).

Species

48-hour A bahia

96-hour M. beryllina

Oil

BMC

BMC

Oil dosing

25g/L

25g/L

100% WAF cone at time Ohr (mg TPH/L)

8.74

8.74

Control

0.00

0.00

Treatment 1 - 6.25 % WAF

0.55

0.55

Treatment 2 - 12.5 % WAF

1.09

1.09

Treatment 3 - 25 % WAF

2.19

2.19

Treatment 4 - 50 % WAF

4.37

4.37

Treatment 5 - 100 % WAF

8.74

8.74

LCso (%)

>100%

>100%

(CI',v> „)

--

--

LCso (mg/L)

>8.74

>8.74

(Cl95°b)

—

—

LCso (%) *

105

108

(Cl95°b)

83 - 127

73 - 143

LC50 (mg/L) *

9.18

9.4

(Cl95°b)

7.3 - 11.1

6.4 - 12.5

LC20 (%)

88.8

92.9

(Cl95°b)

43 - 133

64.6 - 121.3

LC20 (mg/L)

7.7

8.1

(CLv' „)

3.8 - 11.7

5.6 - 10.6

Observed mortality at endpoint in 100% WAF

40% mortality

33.33% mortality

* extrapolated value

EPA/600/X-23/356

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TPH (mg/L)

Figure 2. Percent survival as a function of Total Petroleum Hydrocarbons (TPH) for A. bahia and
M. beryllina test species.

SUMMARY

Reported here are the data results of the physical-chemical properties, aquatic toxicity, and
dispersant effectiveness of Bryan Mound Crude (BMC) oil. BMC has been selected as a new
reference oil for the National Oil and Hazardous Substances Pollution Contingency Plan Product
Schedule (NCPPS; 88 FR 38333) for the EPA Office of Land and Emergency Management (OLEM)
Office of Emergency Management (OEM).

APPENDIX

Appendix 1. Report of Toxicity Tests Bryan Mound Oil Water Accommodating Fraction
Appendix 2. Brian Mound September 2022 Crude Assay Report

REFERENCES

35 FR 8508 (1970) The Council on Environmental Quality (CEQ) published in the Federal Register a

National Oil and Flazardous Materials Pollution Contingency Plan, June 2, 1970.

38 FR 21243 (1973) Assignment of functions under section 311 of the Federal Water Pollution Control
Act, as Amended, Executive Order 11735, August 3, 1973.

EPA/600/X-23/356

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56 FR 54757 (1991) Implementation of Section 311 of the Federal Water Pollution Control Act of
October 18, 1972, as Amended, and the Oil Pollution Act of 1990, Executive Order 12777,
October 22, 1991.

59 FR 47384 (1994) National Oil and Hazardous Substances Pollution Contingency Plan; Final Rule.
September 15, 1994.

88 FR 38333 (2023) National Oil and Hazardous Substances Pollution Contingency Plan; Product
Schedule Listing and Authorization of Use Requirements; Final Rule, December 11, 2023.

Barron, M.G., L. Kaaihue (2003) Critical evaluation of CROSERF test methods for oil dispersant toxicity
testing under subarctic conditions. Marine Poll. Bull. 46:1191-1199.

Barron, M.G., R.N. Conmy, E. Holder, P. Meyer, M.M. Wilming (2018) Toxicity of Cold Lake and Western
Canadian Select dilbits to standard aquatic test species. Chemosphere 191:1-6.

Haines, J.R., K.M. Koran, E.L. Holder, A.D. Venosa (2003) Protocol for laboratory testing of crude oil
bioremediation products in freshwater conditions. J. Indust. Microbiol. 30:107-113.

Ritz, C., F. Baty, J.C. Streibig, D. Gerhard (2015) Dose-response analysis using R. PloS one 10, e0146021.

Sorial, G.A., A.D. Venosa, K.M. Koran, E. Holder, D. King (2004a) Oil spill dispersant effectiveness
protocol - Part I Impact of operational variables. ASCE J. Environ. Eng. 130:1073-1084.

Sorial, G.A., A.D. Venosa, K.M. Koran, E. Holder, D. King (2004b) Oil spill dispersant effectiveness

protocol - Part II Performance of the revised protocol. ASCE J. Environ. Eng. 130:1085-1093.

Spotte, S., G. Adams, P.M. Bubucis (1984) GP2 medium is an artificial seawaterfor culture or
maintenance of marine organisms. Zoobiol. 3:229-240.

US EPA (2009) Method 524.3, V.l: Measurement of Purgeable Organic Compounds in Water by Capillary
Column Gas Chromatography/Mass Spectrometry, EPA 815-B-09-009.

US EPA (2018) Analysis of Semivolatile Organic Compounds by GC/MS, G-LMMD-SOP-1209-0.

Venosa, A.D., D.W. King, G.A. Sorial (2002) The baffled flask test for dispersant effectiveness: a round
robin evaluation of reproducibility and repeatability. Spill Sci. Technol. Bull. 7:299-308.

EPA/600/X-23/356

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APPENDIX 1

EPA/600/X-23/356

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Prepared for:

Pegasus Technical Services
26 W. Martin Luther King Dr
Cincinnati, OH 45268

. PEGASUS TECHNICAL SERVICES, INC. g

o Combining Management and Technical Consulting Services	^	^

Prepared by:
Hydrosphere Research

% pro^S

Test Location:
11842 Research Circle
Alachua, FL 32615

Contact Information:

Peter R. Meyer, Lab Director
(386) 462-7889
pmeyer(5> hydrosphere .net
www.hydrosphere.net

Test Number:

PEG-01 22218

Tests Conducted:

48-Hour Acute Definitive Bioassay Studies with the mysid shrimp (Americamysis bahia)

96-Hour Acute Definitive Bioassay Studies with the inland silverside minnow [Menidia berylliria)

Samples Tested:

Bryan Mound Oil WAF

PEG-01 22218 revOl Page 1 of 44

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Report of Toxicity Tests Performed for Pegasus Technical Services

Bryan Mound Oil WAF

Abstract

At the request of Pegasus Technical Services, Hydrosphere conducted a series of bioassay tests
to determine the potential aquatic acute toxicity effects of the Bryan Mound Oil Water
Accommodating Fraction. Acute studies were conducted using the mysid shrimp (Americamysis
bahia) and the inland silverside minnow (Menidia beryllina).

These tests were conducted in a manner consistent with EPA methods. Hydrosphere Research
believes the results are true and accurate.

Revision 1

A data entry error was discovered after the original report was issued. A note has been added to
the data sheet in appendix C, titled "Acute Saltwater Method (EPA-821-R-02-012, Method
2007.0)". The note corrects the data entry for the 100%, C replicate at 24 hours. The original
value was recorded as "64" and should have been 8. This changed the 24-hour percent survival
from 53% to 60%. The error does not change the report 48-hour LC50. By extension, Table 4 was
also corrected in this revision.

11/02/2023

Peter R. Meyer, Laboratory Director

Date

PEG-01 22218 revOl Page 2 of 44

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Table of Contents

Abstract	2

Table of Contents	3

Table of Figures	3

Introduction	4

Materials and Methods	4

WAF Preparation Figure 1. Bryan Mound Oil	4

WAF Sampling	6

Test Organisms	6

Test Methods	7

Results	8

Bryan Mound Oil WAF Test Results	8

Quality Assurance	9

Standard Reference Toxicant Test Results	9

Summary and Conclusions	10

References	10

Appendix A. Sample Shipping Labels

Appendix B. 48 & 96-hour Acute Raw Data Sheets & Statistical Results for the Range Finder
Studies

Appendix C. 48 & 96-hour Acute Raw Data Sheets & Statistical Results for the Definitive Studies
Appendix D. Reference Toxicant Data for All Test Species

ures

Table 1. WAF Stock Solutions and Test Solutions Sub-Sampling	6

Table 2. Test Organism Information	6

Table 3. Summary of Test Methods	7

Table 4. 48-hour Acute A. bahia Survival	8

Table 5. 96-hour Acute M. beryllina Survival	9

WAF Preparation Figure 1. Bryan Mound Oil	4

Figure 2. WAF systems prior to covering for light blockage	5

Figure 3. 48-hour Acute A. bahia Survival	8

Figure 4. 96-hour Acute M. beryllina Survival	9

PEG-01 22218 revOl Page 3 of 44

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Introduction

The Bryan Mound oil was shipped from Pegasus Technical Services to Hydrosphere Research.
Hydrosphere Research received the sample in good condition.

Using the Bryan Mound oil, Hydrosphere Research prepared Water Accommodating Fractions
(WAF) in synthetic seawater. The WAF solutions were used to conduct acute effect concentration
studies using the mysid shrimp (Americamysis bahia) and the inland silverside minnow (Menidia
beryl Una).

The laboratory bench sheets for the WAF range finding studies are included in Appendix A. The
laboratory bench sheets for the WAF solutions effect concentration determination studies are in
Appendix B. The Standard Reference Toxicity Tests are in Appendix C.

Materials and Methods

WAF Preparation

The WAF materials were prepared from control water and
the Bryan Mound oil. The control water was synthetic
seawater (SSW). Control water and a magnetic PTFE stir bar
were added to a glass cylinder and the cylinder was placed
on a stir plate. The cylinder was covered with a glass plate
with a silicone gasket to seal the contents of the cylinder.
The cover had two access holes fitted with a glass tub in one
and a silicone stopper in the other. The glass tube allowed
access to the WAF sample below the oil line. Bryan Mound
oil was slowly added to the cylinder at the designated
concentration of 25 gm/L. Stirring was initiated to energize
the system. The goal was to achieve a shallow vortex of oil
into the aqueous layer without having the oil break apart
into globules. The goal was to have a vortex that was 20%
of the aqueous layer's height. The system was carefully
watched to ensure that the vortex did not break apart. The
vortex was reduced to approximately 15%. Figure 2 below
• lustrates the setup. The cylinder and cover were wrapped
with aluminum foil to keep the system dark. One section
was left loose to allow observations.

Figure 1. Bryan Mound Oil

^•Van Mound'

Report, TX

'¦« Cor*-=' v- I

iSl£"> N ;oonv. D

*" Re' -jarchVid lVf

lifter King J' ^
. iBTWI, QH 45.68

Stirring continued for 18 hours at room temperature. The system was checked periodically to
ensure the vortex was stable. The stir plate was turned off after IS hours and checked for any
evidence that oil globules had broken off from the vortex during stirring. The system was then
allowed to settle for 6 hours. At the end of the settling period, the WAF was collected by
siphoning it with silicone tubing through the access tube in the glass plate cover into an aluminum
foil wrapped glass jar. The jar was covered with a foil lined lid to prevent loss of volatile chemicals.

PEG-01 22218 revOl Page 4 of 44

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The collected WAF sample preparation was recorded in the lab's "Chemical & Solutions Logbook"
and assigned the number 23138-SLN, Other sample preparation notes were recorded on a
"Laboratory Notes" bench sheet located in appendix B. These samples constitute the 100% WAF
samples recorded on the test bench sheets, For the exposure test solutions, dilutions of the 100%
WAF sample were prepared with the appropriate control waters.

Figure 2. WAF systems prior to covering for light blockage.

PEG-01 22218 revOl Page 5 of 44

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WAF Sampling

WAF stock solutions and test solutions were subsampled and shipped to Pegasus Technical
Solutions, Inc. All samples were preserved with HCI. Below is a table which summarizes the WAF
stock solutions and test solutions.

Table 1. WAF Stock Solutions a Solutions Sub-Sampling

Hydrosphere
Solution ID

Hydrosphere Solution Description

Date
Solution
Prepared

Date
Sampled

Date
Shipped to
Pegasus

SSW-5982

BryanMoundOil-WAF/230510/Salt/AcuteEC, Control, @Ohr/SS&MS

5/10/23

5/15/23

23139-SLN

BryanMoundOil-WAF/230510/Salt/AcuteEC,6.25%,@0hr/SS&MS

5/10/23

5/15/23

23138-SLN

BryanMoundOil-WAF/230510/Salt/AcuteEC,100%,@0hr/SS&MS

5/10/23

5/15/23

23143-SLN

BryanMoundOil-WAF/230512/Salt/AcuteEC, Control, @48hr/MS

5/12/23

5/15/23

23144-SLN

BryanMoundOil-WAF/230512/Salt/AcuteEC,6.25%,@48hr/MS

5/12/23

5/15/23

23145-SLN

BryanMoundOil-WAF/230512/Salt/AcuteEC,100%,@48hr/MS

5/12/23

5/15/23

23146-SLN

BryanMoundOil-WAF/230514/Salt/AcuteEC, Control, @96hr/SS

5/14/23

5/15/23

23147-SLN

BryanMoundOil-WAF/230514/Salt/AcuteEC,6.25%,@96hr/SS

5/14/23

5/15/23

23148-SLN

BryanMoundOil-WAF/230514/Salt/AcuteEC,100%,@96hr/SS

5/14/23

5/15/23

UPW(0.055|iS)

Field Reagent Blank (FRB)

5/10/23

5/15/23

Test Organisms

The test organisms used in this study were the mysid shrimp (A. bahia) and the inland silverside
minnow (M. beryllina). The mysid shrimp (A. bahia) test organisms were cultured in-house. The
inland silverside (M. beryllina) test organisms were commercially obtained (Aquatic Indicators,
St. Augustine, FL).

The test organism information is described in Table 2. Test Organism Information.

Test Organism

Source

Organism Age

A. bahia

In-house Cultures

3 days

M. beryllina

Commercially Obtained

12 days

PEG-01 22218 revOl Page 6 of 44

-------
Test Methods

The summary of the test methods used are described in the following table:

Acute A. bahia

Acute M. beryllina

Test method

EPA-821-R-02-012, Method 2007.0

EPA-821-R-02-012, Method 2006.0

Test type

Static non-renewal

Test duration

48 hours

96 hours

Salinity

20 ± 2%o

Renewal

Temperature

25 ± 1 °C. Test temperatures must not deviate (maximum minus minimum
temperature) by more than 3 °C during the test.

Light quality

Ambient laboratory illumination

Light intensity

10-20 (E/m2/s)

Photoperiod

16 h light, 8 h darkness, with phase in/out period recommended

Test chamber size

500 mL

1 L

Test solution
volume.

200 mL

Age of test
organism

1-5 days

9-14 days

No. organisms per
test chamber

No. of replicate
chambers per
concentration

Feeding regime

Refer to specific feeding procedures provided in each test method

Aeration

None, unless DO falls below 4.0 mg/L, then aerate all chambers. Rate: <100

bubbles/minute

Physical /
Chemical
Measurements

Daily temperatures were measured in one replicate for each test concentration.
Exposure test solutions were analyzed daily for pH, dissolved oxygen, and either

conductivity or salinity.

Test
concentrations

5 exposure concentrations and a control

Test acceptability
chambers per
concentration

>90% survival in controls

All statistical calculations were made using CETIS® (Tidepool Scientific Software, McKinleyville,
CA). The sample statistical results are in Appendices A and B.

The bioassay tests were performed at Hydrosphere Research, 11842 Research Circle, Alachua, FL
32615; telephone number (386) 462-7889. The laboratory is NELAC/P certified by the State of
Florida Department of Health and Rehabilitation Services (E82295).

PEG-01 22218 revOl Page 7 of 44

-------
Results

Bryan Mound Oil WAF Test Results

The results of the acute definitive tests are summarized in the tables and figures below. The raw
data and bench sheets are included in Appendix B.

Table 4.48-hour Acute A. bahia Survival

48-hour M. bahia % Mean Survival



% WAF

0 Hours

24 Hours

48 Hours

<

Control

100

100

100

o

6.25

100

100

100

"D
C

12.5

100

100

100

D
O

25

100

100

100

2

c

50

100

100

100

ro

>-

100

100

60

60

CO

LC50

-

-

>100%

Figure 3.48-hour Acute A. bahia Survival

M. bahia Survival
100 •	•	«	•-

80

1 60
>

= 40

i/i

aS 20

0

Control 6.25 12.5 25 50 100
% WAF

\

PEG-01 22218 revOl Page 8 of 44

-------
Table 5.96-hour Acute M. beryllina Survival

96-hour

VI. beryllina % Mean Survival

Bryan Mound Oil WAF

% WAF

0 Hours

24 Hours

48 Hours

72 Hours

96 Hours

Control

100

100

100

100

100

6.25

100

100

100

100

100

12.5

100

100

100

100

100

25

100

100

97

97

97

50

100

100

100

100

100

100

100

70

67

67

67

LC50

-

-

-

-

>100%

Figure 4.96-hour Acute M. beryllina Survival





M. beryllina Survival



100

• •	T A ¦»



80

\



\

03

60
40
20



>
>

	

3

CO

as







0





Control 6.25 12.5 25 50 100
% WAF

Quality Assurance

All phases of the study including, but not limited to, sample handling and storage, glassware
preparation, test organism culturing/acquisition and acclimation, test organism handling during
test, and maintaining appropriate test conditions were conducted per the applicable method. No
known deviations were noted during the study.

All chemicals were certified products used before expiration dates (where applicable). All
identification, service, and calibration information pertaining to laboratory instruments is
recorded in calibration and maintenance logbooks. The bioassay tests were acceptable tests
based on control performance and test conditions.

Standard Reference Toxicant Test Results

The results for the standard reference toxicant tests are in Appendix C which includes the control
charts, statistics, and raw data.

PEG-01 22218 revOl Page 9 of 44

-------
Surtiri •[ * 100% for the
mysid shrimp (Americamysis bahia) and a 96-hour LC50 of >100% for the inland silverside minnow
(Menidia beryllina).

Dissolved oxygen, temperature, and pH remained within the limits established in the test
methods. The Acute and Chronic Standard Reference Toxicant tests demonstrated that the test
organisms used in this study were of acceptable health and sensitivity.

No unusual observations or deviations from standard test protocol were noted. No unusual
qualitative test organism behaviors were observed in the test exposures. These test results only
relate to the samples described in this report and meet all requirements of NELAC.

References

U.S. Environmental Protection Agency. Methods for Measuring the Acute Toxicity of Effluents and
Receiving Waters to Freshwater and Marine Organisms. Fifth Edition. EPA-821-R-02-012. October
2002.

Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part
136). EPA 821-B-00-004. July 2000.

Handbook of Analytical Quality Control in Water and Wastewater Laboratories. EPA-600/4-79-
019. March 1979.

Chemical and physical parameters reported herein were determined by methods described in
Methods for Chemical Analysis of Water and Waste. EPA 600/4-79-020. March 1983.

Comprehensive Environmental Toxicity Information System (CETIS®), Version 1.9.7.9, Tidepool
Scientific Software, McKinleyville, CA.

PEG-01 22218 revOl Page 10 of 44

-------
Appendix A.

Sample Shipping Labels

PEG-01 22218 revOl Page 11 of 44

-------
'.f.

' *

|}'*£i *!. 	

iarAup(2022 22:004626

1 OF 1

DWT. 8,8,10

RETURN USEPA — OARM

JENNIfER TANT	5 LBS

(513)569-7185
US EPA

26 W. MARTIN LUTHER KING DRIVE
CINCINNATI OH 45268-0001

SHIP TO:

PETER MEYER

HYDROSPHERE research env services
11842 RESEARCH CIRCLE

ALACHUA Fl/32615-6817

* ,-cps-pv'Ce* X-tie't'l- ^ I V'OO mr>
C.WZAA Via fc* ^T-O f 1 "C&M 	____

^^^TFL3260-02

UPS GROUND

TRACKING #; 1Z A43 F24 03 5056 0256

hazardous, MATER I als - ground only
HAZ#: U fell 267 -

Reference Name: Robvn Conmy		

^ S~ OdUj

9«suW»'f|a

„.SS5i=-

tlftflBflM, IHMWim—t-—	—	v,	. .

PEG-01 22218 revOl Page 12 of 44

-------
Appendix B.

48 §• 96-hour Acute Raw Data Sheets II Statistical Results for the Range Finder Studies

PEG-01 22218 revOl Page 13 of 44

-------
Hydrosphere

research

Acute Saltwater Method (EPA-821-R-02-012, Method 2007.0)

Survival (

Client:

Pegasus Technical Services, Inc.



Control Water:
Diluent:

ssw



Initiaton Date: % % | %\ Termination Date: 3/10 JZ$

Code: PEG-01

Job:

22218

ssw

Sample Description:

	^	—	—	

Species;
ID#:

Mysidopsis bahia

Code:

MS



Test Vessel:
Test Volume:

500-mL Glass Jar



ProducCTuoi oil WRD WAF (25 gm/L)
Test: Range-finder (RF)

I/O A8e:

^fLab, O Cora®

200-mLs per replicate



%





Live Counts



Sample

R

E
P

V I

P-

V



Description

WAF

Oh

24h

48h







A

10

1°

10



Control

0

B

10

10

10







C

10

10

\0







A

10

*0

10





0.01

B

10



in







C

10

\o

\o







A

10

\0

i(7





0.1

B

10

10

IQ







C

10

(0

\0







A

10

Is

\r>



>

1

B

10

lO

ID



>T5



C

10

to

\o







A

10

to

VO





10

JB

10

la

iQ







C

10-

40

ID







A

10

10

10





100

B

10

It)

\0







C

10

i0

so



Meter ID Mt

qW 4-z,)

4tM



Initials:

0 m





Time:

1 ioo



Control ID:

$



a jh

i



Diluent

ID:





Working Stock ID:



* 8LN

us?

o IS

*

Oil ID

ID:



22071

•CHM

3 o

Random-
ization
Template #



Feeding Type;



Artemia {concentrated slurry)

Amount:



2-drops (0,1-mL) 2-limes» daily



W«s







Time;



\SS>

\L<°

—



pH

facceotabie ranee for a valid test is 6 to 91

Dissolved Oxygen (mg/L)

faccBDtable minimum fix a valid test is 4.0-mg/L)

0b
new

24h
old solution

48h
old solution



Oh
new

24h
old solution

48h
old new



A

1,1

^-O

ft.fr
31 to



7,5

n.H





1'0

74

^¦\



7<^

-i.H

a

1.0

%0

n

'7, ^

1M

l\

1,0





1M

~"V3

s.\





w

1H

"1.3





%.\

%,0

<*-\



V\

7, ^







cn\





m





tot

PM

R 6-



fo£-

p#\

KC-



Notes & Comijif jits





Sample
Description

% 1

WAF |







Control

0



WAF

0.0I



0.1



I



10

Pkotoperiod is 16L:8D, UlirataiatSonis ambient (50 to 100 ftcd)



100

CD Check Box: Lab = In-House Reared, Com - Commercially obtained

Meter ID #:



Salinity (%») 1



ttO±2?^FedRee/Vol8Q.Nol4/Jan20i5) ||

Oh

24h

48h



new

old solution

old new



M

70,5





n

20.6





'p%

203

aua



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?o.4

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mi





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Temperature (°C)



/WhiIbHIk ranpe for a valid te

tis25ilQC)

0

24

48





^S". V

,7





2>", I





%>

T5 .1

U .7



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. MU

Mil



Veisioa 1 (13036-DCF) F:\Glients\PEG-01, PegasusTechServ\22218,

Bryan Mound WARBench&AnalyticaM) 22G71CHM (BiyanMoundOil)M) RFVPEQ-01 22039.MS.48.ASND.EPA2007.vl.RF(22018-CHM)

PEG-01 22218 revOl Page 14 of 44

-------
CETIS Analytical Report

Report Date;
Test Code/ID:

14 Mar-23 12:11 (p 1 of 2)

IPEG-01 22218MSA7 16-4365-1580

Reference Toxicant 96-h Acute Survival Test

""

:	 	

Hydrosphere Research

Analysis iD: 11 -3875-8435
Analyzed: 14 Mar-23 12:11
Edit Date: 14 Mar-23 12:10

Endpoint: 48h Survival Rate

Analysis: Linear Interpolation (ICPIN)

MD5 Hash: 92B8802B84EfiOA6B6D096CCFFF74D484

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Batch ID:.- — 18r07Q5-0917.	

Start Date: 08 Mar-23 16:00
Ending Date: 10 Mar-23 15:35
Test Length: 48h	

J

Test Type: Survival (48h)

Protocol: EPA/821/R-02-012 (2002)
Mysidopsis bahia f

| Species
Taxon:-

Analyst: Lab Tech

Diluent: -Mod Hard Synthetic Water

Brine: '32M/ £C1 !"-h

Malacostraca

Source:

In-House Culture

Age: 5c)

Sample ID: 18-0787-2617
Sample Date: 08 Mar-23
Receipt Date: 08 Mar-23
Sample Age: 16h

Code:	6BC1F269

Material:	Product
CAS (PC):

Client:	Pegasus

Project: Product Toxicity Test
Source: Pegasus Technical Services
Station: -Pnei-ei-WRD W£F

	0

Linear Interpolation Options
X Transform Y Transform

Seed

Resamples

Exp 95% CL Method

Log(X+1)

Linear

1528842 200

Yes

Two-Point Interpolation

Point Estimates
Level mg/L

95% LCL S5% UCL

LC50 >100

48h Survival Rate Summary

Calculated Variate(A/B)

Isotonic Varlate

| Conc-mg/L

Code

Count

fMean ']

Median

Min

Max

CV%

%Effect

A/B

Mean

%Effect

i 0

D

3

1 1.0000 J

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

J 0.01



3

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

0.1



3

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

1



3

1,0000

1.0000

1.0000

1.0000

0,00%

0.00%

30/30

1.0000

0.00%

10



3

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

100



3

1.0000

1.0000

1.0000

1.0000

0.00%

0,00%

30/30

1.0000

0.00%

48h Survival Rate Detail





















Conc-mg/L

Code

Rep 1

Rep 2

Rep 3















0

D

1.0000

1.0000

1.0000















0.01



1.0000

1.0000

1.0000















0.1



1.0000

1.0000

1.0000















1



1.0000

1.0000

1.0000















10



1.0000

1.0000

1.0000















100



1.0000

1.0000

1,0000















48h Survival Rate Binomials
Conc-mg/L	Code Rep 1

Rep 3

Rep 2	.....

V-10/10 Nj 10/10	^ 10/10

^10/10 —,10/10	^10/10

^ 10/10 10/10	10/10

^ 10/10 \) 10/10	w10/10

X/10/10	xj 10/10

^ 10/10	10/10

0

0.01
0.1

1

10
100

D

10/10
V* 10/10

003-737-857-6

CETIS™ v1.9.7.9

Analvst	QA:	M*-3 I

PEG-01 'ims revOl Page 15 of 44

-------
CETIS Analytical Report

Report Date:	14 Mar-23 12:11 (p 2 of 2)

Test Code/ID; PEG-01 22218MSA / 16-4365-1580

Reference Toxicant 96-h Acute Survival Test



Hydrosphere Research

Analysis ID:
Analyzed:
Edit Date:

11-3875-8435
14 Mar-23 12:11
14 Mar-23 12:10

Endpoint: 48h Survival Rate

Analysis: Linear interpolation (ICPIN)

MD5 Hash: 92B8802B84E90A6B6D096CCFFF74D484

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Graphics

003-737-857-6

CETIS™ v1.9.7.9

Analyst		 QA:	

PEG-01 22218 revOl Page 16 of 44

-------
Hydrosphere

research

Acute Saltwater Method (EPA-821-R-02-012, Method 2006.0)

Survival

Client:

Pegasus Technical Services, Inc.



Code: PEG-01

Job:

22218

Species:
ID#:

Menidia beryllina

Code:

SS

toys

Age:

1 U

~ Lab, g/Com©

Control Water:
Diluent:

ssw



Initiaton Date:



Termination Date:

%/Wtl 1

ssw

Sample Description:

Test Vessel:
Test Volume:

1-L Glass Jar



\£J

Product:-Fuel oil WRD WAF (25 gm/L)
Test; Range-finder (RF)

200-mLs per replicate

Sample
Description

%

R

I

:

Live Counts

u

R.

f



5,-

WAF |

0

24

48

72

96

Control

0

A
B

€
A
B
C
A
B
C
A
B
€
A
B
C

A
B
C

10

(0

to

\o

l»

10

10

(0

|0

Y

10







1

WAF

0.0 i

10

,0

SO

\0

fo

10

to

(0

lo

10

10

f

?

<=l

*'

0.1

10

^k

JO

(O

[0

10

to

to

IO

ID

10

(«

w





10

|0

10

10

m

10

ID

10

10

m

10

10

10

10

to

IO



1

100

10

1'

f



1

10

%%

e

%

8

10

10

V

°l

1

Meter ID #;



HtA



m

HM

Initials:





PM

p-



Time:

ISMS

\W

W'K

i m

iSjl!

Control ID:

SliT-

tlf

Diluent ID:



t-

s; §»

Working Stock TD:

-SLN

|. g
3 Z

Oil ID ID;

22071 •chm

si

Random-
ization
Template #



Fading Type;

Amount;

Timet

Artemis (concentrated slurry)



pH

(acceptable ranee for fl valid test is 6 to 9}

1 Dissolved Oxygen (mg/L)

1 (acceptable mWrnian for a valid test is 4.G*m£t/L)

Salinity (%o)

f20£2%o; Fcdftes/Vol80. Noli I Jnn2015)

0 I 24
new 1 old solution

48

old solution

72

old solution

96
old

| 0
j new

24

old solution

4S

old solution

72

old solution

96
old

fi
new

24

old solution

48

old solution

72

old solution

96
old

ffc\

7, $

7*

7.^





7,3

7,4

7.6

m

1#S

Z0.S

2PM

20»1

?ci

ip

.S



V©

to

2.\





73



7.4 ¦

73



"lo.l

20S

20. S

2CI

t\



10

V



b

73

7 3

7.4

72



10 y

10 i



2af



%.o

%,0



SA



73

7.3

7.^

72

-"Si

y>H













14 U.



hn

c/H

^i

W^L-

0/*





p/A

Notes & Comments



Sample
Description

%
WAF

1'e

facccnwhie

nperature I

unse for a valid te

UQ

t«25iFQ

«D r PS\ 3/7/2* .



0

24

48

71

96

m hi, ®

Control

0



VS.l

•2^.6

iso



1

WAF

0.01

2^.1





1S,\

is.y



0.1





"iSv (3

1

y.t



1

"2Sj3

.M



zs.^

^.5



10

"xS.o





ZS"



Photoperiod is 16L:8D, lUuminiation is ambient (50 to 100 Itcd)



100

AS-/





0-53

2?J

© Check Box: Lab = In-House Reared, Com = Commercially obtained

Meter ID #;







^2-S

4^4

Version 2 (13040-DCF)	F:\CUents\PEG-01, PegasusTechServV22218, Bryan Mound WAFNBetich&Analytica3\])22071CHM(BryanMoundOil)M)RjF\PEG-01 22039.SS.96.ASRD.EPA2006.v2.RF(22018-CHI^.q 22218 revOl Page 17 of 44

-------
CETIS Analytical Report



Report Date:
Test Code/ID:

14 Mar-23 12:12 (p 1 of 2)
J PEG-oT 222187 10-1101-7421

Reference Toxicant 96-h Acute Survival Test



Hydrosphere Research

Analysis ID: 15-2719-7493
Analyzed: 14 Mar-23 12:12
Edit Date; 14 Mar-23 12:05

Endpoint: 96h Survival Rate

Analysis: Linear interpolation (ICPIN)

MD5 Hash: 6A22360F0EE35D2B537C2F23E926E9CB

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Batch ID:

•{2-1014-8483

Start Date: 08 Mar-23 15:45
Ending Date: 12 Mar-23 15:25 I J Species
Test Length: ,96h

Test Type: Survival (96h)

Protocol: EPA/821/R-02-012 (2002)

J

Menidia beryllina

Taxon;

Aotinopterygii

Analyst:	Lab Tech
Diluent:

Brine:	5F"'

Source:	In-House Culture

; Water

Age: J2.cl

Sample ID: 05-9696-9274
Sample Date: 08 Mar-23
Receipt Date: 08 Mar-23
Sample Age: 16h

Code:	2395073A

Material:	Product
CAS (PC):

Client:	Pegasus

Project: Product Toxicity Test
^Sourjs^—Pegasus-Technical Services

Station: -Fuel Oil WRD WAF
mYlri a*.' I























Linear Interpolation Options



















X Transform

Y Transform

Seed Resamples

Exp 95% CL Method









Log(X+1)

Linear

434087 200



Yes Two-Point Interpolation







Test Acceptability Criteria

TAC Limits















Attribute

Test Stat

Lower

Upper



Overlap

Decision









Control Resp

0.9333

0.9

»



Yes

Passes Criteria









Point Estimates I



















Level mg/L I 95% LCL

95% UCL















^.LCSO >100 j —

96h Survival Rate Summary









Calculated Variate(AZB)





Isotonic Variate

Conc-mg/L

Code

Count

f Mean

|

Median

Min Max CV%

%Effect

A/B

Mean

%Effect

0

D

3

f 0.9333

—r

0.9000

0.9000 1.0000 6.19%

0.00%

28/30

0.9500

0.00%

0.01



3

0.9333



1.0000

0,8000 1.0000 12.37%

0,00%

28/30

0.9500

0.00%

0.1



3

0,9667



1.0000

0.9000 1.0000 5.97%

-3.57%

29/30

0.9500

0.00%

1



3

0,9667



1.0000

0.9000 1.0000 5.97%

-3.57%

29/30

0.9500

0.00%

10



3

0.9333



1.0000

0,8000 1.0000 12.37%

0.00%

28/30

0.9333

1.75%

100



3

0.8667



0.9000

0.8000 0.9000 6.66%

7.14%

26/30

0.8667

8.77%

96h Survival Rate Detail



















Conc-mg/L

Code

Rep 1

Rep 2



Rep 3











0

D

1.0000

0.9000



0.9000











0.01



1.0000

1.0000



0.8000











0.1



1.0000

1.0000



0.9000











1



0.9000

1.0000



1,0000











10



1.0000

1.0000



0.8000











100



0,9000

0.8000



0,9000











96h Survival Rate Binomials



















Conc-mg/L

Code

Rep 1

Rep 2



Rep 3











0

D X10/10

Si 9/10

S.

9/10











0.01



O

o

v.10/10

S-8/10











0.1

X10/10

^10/10

V/9/10











1



9/10

X10/10

10/10











10



10/10

Sj 10/10



8/10











100



9/10

8/10



9/10











003 737-857-6	CETiS™ v1.9.7.9	Analyst: 	 QA:	;	'

003 /if b5/ b	PEG-01 22218 revOl Page 18 of 44

-------
CETIS Analytical Report

Report Date:
Test Code/ID:

14 Mar-23 12:12 (p 2 of 2)
PEG-01 22218/10-1101-7421

Reference Toxicant 96-h Acute Survival Test

Hydrosphere Research

Analysis ID: 15-2719-7493
Analyzed: 14 Mar-23 12:12
Edit Date: 14 Mar-23 12:05

Endpoint: 86h Survival Rate	CETIS Version: CET1Sv1.9.7

Analysis: Linear interpolation (ICPIN)	Status Level: 1

MD5 Hash: 6A22360F0EE35D2B537C2F23E926E9CB Editor ID:	003-737-857-6

Graphics

003-737-857-6

CETISiu V1.9.7.9

QA:	

. rcvOi Page 19 of 44

-------
Hydrosphere

research

Client:

Pegasus Technical Services

Laboratory Notes

Job

Code:

PEG-01

22218

Task Title:

25 gm/L WAF prep

Task Page 1 of 2

1) Product Name:

Bryan Mound Oil

Tech Initials:

PRM

2) Lab ID:

22071

• CHM

Date:

3/7/2023



OIL ~ WAF Preparation (Rangc-flnifing Test)

oil mass for WAF

25



0.01
0.1
1

10
100

Bioassay WAF (mLs)

Saltwater Tests

SS

600

0-06
0.6
6

60
600

666.66

MS

600

0.06
0.6
6
60
600

666.66

Both
1200

0.12
1.2
12
120
1200

1333.32

Number of renewals;
WAF for renewals (mL)
Oil chem for WAF
BTEX for WAF
Total WAF (ni.)

Rounded
Mass of GO (gm)

1

1333.32

1000
80

2413

2700

~] 2613

67.5

Cri&SiS Cylinder	laside dimsmjoss (measured, Hot cafe!)

6	diameter (in)

8

20.32

Jheight (in)
height (cm)

14.3

18.7

3.0

cm (width)
cm(hei|}t)
L (volume)

Item 1
Item 2
Item 3

OiI.~WAF

OIL - WAF Preparation (Runge-finding Test)

Saltwater SS & MS

Item 4
Item 5
Item 6
Item?
Bern 8
Item 9
Item 10

WAF needed
Cylinder
WAF height

height+20%
cover ht of
is what %?j
20% vortex

2700

3003.3

16.81

20.17
18.7

JiaLs
jaiLs

1cm (from inside bottom, up)
cm (fiom inside bottom, up)

cm (from inside bottom, op)

3= 11.2 ] % (shoot for 20%!)

13.45 cm (from inside- bottom, tip)

OIL

from "Mass of Oil (gas)"

Item 11
Item 12
Item 13

67.5

Oil needed)
Diameterf 14.30
Oil ttfcJmessI 0.42

Labor Hours to perform tasks on this page:

NA

Version 2 (1319ti0fi©®Mi,P6g™^ftehs«i^j»,Biy«nMaiiidWAiwen«h&Aii4yiicrtt)22O7i'CHM®«yanMoiiii«ffl)(iOwwBG-oi222i8.i)LN.pB0ogfpg5i2W5®vOl Page 20 of 44

-------
i Hydrosphere

research



Client:

Pegasus Technical Services

Laboratory Notes
Code:

PEG-01

Job:

22218

Task Title

25 gm/L WAF prep



Task Page 2 of 2

1) Product Name:

Bryan Mound Oil

Tech Initials:

PRM

2) Lab ID:

22071

¦ CHM



Date:

3/7/2023

WAF Mixture

Oil Mass:

25

gms/L 1) Initiation 18-hours of Mixing on Stir Plate (Target

Oil ID:

22071

¦ CHM is to achieve a 20% vortox)

Water Volume:

2.70

L

—

Water ID:

5917



ssw

Date:

3/7/2023



Product (unit/L):

NA

E
B

R"

n

gm

Time;

14:10

£

??

PRM



Product ID:

NA

• CHM Product Target:

#VALUE!

c

sa

&

gm



Event Mass (gms) Action Product added:



IS
0

sr

gm



A

Oil Needed

67.5

Tare Cup

Time:

NA



NA



B

Add Oil to Cup ©

68.01

weigh

Vortex Height:

14



%

C

Left in Cup

2.09

weigh



D

Total Oil in Jar

65.92

B-C

2) Terminate Mixing (at 18-hours)

E

Oil needed

1.58

A-D



—

F

Target for Cup

3.67

C+E

Date:

3/8/2023



G

Oil added to Cup

3.68

weigh

Time:

8:05

S

LH



H

Left in Cup

1.82

weigh

System Stable?:

yes



I

Oil added to Jar

1.86

G-H



J

Total Oil in Jar

67.78

D+I

3) Collection of WAF (after 6-hours settling)

K

Oil needed

-0.28

A-(D+H)

Time:

14:15

Bi

1 PRM



L

Target for Cup

1.54

H+K

Solution ID:

23048

• SLN



M

Oil added to Cup



weigh



N

Left in Cup



weigh



—

0

Oil added to Jar

0.00

M-N



Totii 1 Oil in .fill'

67.78

J+O



Q

Percent of Total

100.41%

((D+l+0)/A)*100



Notes & Comments

© Cup is an aluminum foil (acetone rinsed) lined 5.5 oz Solo Cup or DM16 cup

Version 2 (13191-J^fiJmMl,P^ffiT«hServ\2221S,BiyanMoundWArei»dl&ArKl1yt«ii]U)2207l CIBd(B^Oun»il)U)RroEG-012221SJ>LK^ee«t5iJ^J^ev()1 p ^ Qf 44

-------
Appendix C.

48 §• 96-hour Acute Raw Data Sheets II Statistical Results for the Definitive Studies

PEG-01 22218 revOl Page 22 of 44

-------
I Hydrosphere

research

Client:

Pegasus Technical Services, Inc.



Code;

PEG-01

Job:

222 IS

Species:

Mysidopsis bahia

Code;

MS

ID#:



Age:| 3>c\ tV] Lab. ~Com©





3

6.25

12.5

25

50

100

Meter ID ft

Initials:

Time:

Control ID;

Diluent ID;

Working Stock ID;

Oil ID ID:

Random-
ization
Template#

7-

Feeding Type:

Amount:

Time:

Version 1 (13036-DCF)

W

Oh

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

y.

KG

ioOO

PT

24b



w

10



Mi

u>



\o

Ml

b

M.

k>_

U>

i£_



4^



%jL

ts(0

48h



\P

to

10

i 0

10

to

\0

10

to

10

to

15





Acute Saltwater Method (EPA-821-R-02-012, Method 2007,0)

Survival







-sln

22071

¦CHM

Artemia (concentrated starry)

2-drops(0.1-ml.) 2-times, daily

X



i 0$



101(5

-



Control Water:

ssw



Diluent;

ssw

Test Vessel:

500-mL Glass Jar

Test Volume:

200-mLs per replicate



1 PH

1 (acceptable ranae for a valid test is 6 to 91

Oh
| new

24h
old solution

48h
old solution







7,1



tx



i.l



%?-

?,o

l.o



n



"2.0



u



7,1



n

'

S.o



13^



q%







Pan



Notes & Comments

Inltiaton Date:

5 |u>

la.

Termination Date:

sl Il\^%

Sample Description:



Product: Bryan Mound Oil WAF (25 gm/L)
Test: Effect Concentration (EC)





Oh
new

it

i

X

}3

3,1

ii



(acceptable mi ntmum for a valid test is 4.0-mg/L)

24h
old solution

n













C-frpr. jftg- CdU'/i r"

s Usv1^ % . *'

4-#~ i'/i r

r.eoit&A-^'C

V}

Photoperiod is 16L:8D, lllumioiation is ambient (50 to 100 ftcd)
© Check Box: Lab «In-House Reared, Com - Commercially obtained

48h
old new

7,9



v Vkm

F:\Clients\PEG-01.PegasusTechServU22l8, Biyan Mound WAPBench&AnalyticaM)22071'CHM{BtyanMoundOil)\2)ECTEG-01

13

iH

n3

iX

Hli



Sample

%

Description

WAF

Control

0



6.25



12.5

WAF

25



50



100

Meter ID #:

1

Salinity {%#)



BWk FcdRoe / V0I8O. No 141J*i2015>

0b

24h

48h



1 new

old solution

old new



L

2-1,5

20A



1ok



to,?





U"\





k.L

?A$J

zor?



10 b

h>l-

Z&.6



\os

lo^

Zf)'l



3*

lit-

HU



fU



P/n





Temperature (°C)



faecsnlable r

angBforavaltM

2-S J>

2 r. Li











lis



X5.4











U>.Jk

%<>s





HU?

Ml-5

qxC

-------
CETIS Analytical Report

Reference Toxicant 96-h Acute Survival Test

Report Date:	10 Jui-23 14:11 (p 1 of 2}

Test Code/ID: PEG-01 22218MSA/18-1667-8004
Hydrosphere Research

Analysis ID:

00-6458-4846

Endpoint: 148h Survival Rate)

CETIS Version: CETISv1.9.7

Analyzed:

10 Jul-23 14:11

Analysis: Linear Interpolation (ICPIN)

Status Level: 1

Edit Date:

10 Jul-23 14:10

MD5 Hash: DB26147371EC252DF1653D082A0ADFF1

Editor ID:

003-737-857-6

Batch ID:

20-5567-3677

Test Type:] Survival (48h) J

Analyst:

Lab Tech

j Start Date:

10 May-23 16:00 )

Protocol: EPA/821/R-02-012 (2002)

Diluent

Mod-Hard Synthetic Water

/ 		

Ending Date:

12 May-23 15:36

Species: J Mysidopsis bahia f

Brine:



Test Length:

48h

Taxon: Malacostraca

Source:

In-House Culture Age:

Sample ID: 19-9553-9355
Sample Date: 08 Mar-23
Receipt Date: 08 Mar-23
Sample Age: 63d 16h

Code:	76F1839B

Material:	Product
CAS (PC):

Client:	Pegasus

Project:
Source:
Station:

Product Toxicity Test
Pegasus Technical Services



a <*n4

Linear Interpolation Options
X Transform Y T ransform

Seed

Resamples Exp 95% CL Method

Log(X+1)

Linear

1761206 200

Yes

Two-Point Interpolation

Point Estimates
Level mg/L f 95% LCL 95% UCL

LC50 >100 J --
48h Survival Rate Summary

Calculated Variate(A/B)

Isotonic Variate



Mean

Median Min

Max

CV%

%Effect A/B

Mean

%Effect

0

D

3

1,0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

6.25



3

1.0000

1,0000

1.0000

1.0000

0.00%

0,00%

30/30

1.0000

0.00%

12.5



3

1.0000

1,0000

1.0000

1.0000

0,00%

0.00%

30/30

1.0000

0,00%

25 ,



3

1.0000

1.0000

1.0000

1.0000

0,00%

0.00%

30/30

1.0000

0.00%

50 /



3

1.0000

1.0000

1,0000

1.0000

0.00%

0.00%

30/30

1.0000

0.00%

I

100

—>



3

0,6000

0.6000

0.4000

0.8000

33.33%

40.00%

18/30

0.6000

40.00%

48h Survival Rate Detail

Conc-mg/L Code

Rep 1

Rep 2

Rep 3

0 D

1,0000

1.0000

1.0000

6.25

1.0000

1.0000

1.0000

12.5

1.0000

1.0000

1.0000

25

1.0000

1,0000

1.0000

50

1.0000

1.0000

1.0000

100

0.6000

0.4000

0.8000

48h Survival Rate Binomials







Conc-mg/L Code

Rep 1

Rep 2

Rep 3

0 D

10/10

10/10

10/10

6.25

10/10

10/10

10/10

12.5

10/10

10/10

10/10

25

10/10

10/10

10/10

50

10/10

10/10

10/10

100

6/10

4/10

8/10

003-737-857-6

CETIS™ v1.9.7 9

Analyst:	QA:	

PEG-01 222IS revOl Page 24 of 44

-------
CETIS Analytical Report

Report Date:	10 Jul-23 14:11 (p2of 2)

TestCode/iD: PEG-01 22218MSA / 18-1667-8004

Reference Toxicant 96-h Acyte Survival Test	Hydrosphere Research

Analysis ID; 00-6458-4846	Endpoint; 48h Survival Rate	CETIS Version: CETISvi .9.7

Analyzed: 10 Jul-2314:11	Analysis: Linear Interpolation (ICPIN)	Status Level: 1

Edit Date: 10 Jul-23 14:10	MD5 Hash: DB26147371EC252DF1653D082A0ADFF1	Editor©:	003-737-857-6

Graphics

003-737-857-6

CETIS™ v1.9.7.9

Analyst:		 QA:	

PEG-01 22218 revOl Page 25 of 44

-------
Hydrosphere

research

Acute Saltwater Method (EPA-821-R-Q2-0I2, Method 2006.0)

Client:

Pegasus Technical Services, Inc.

Control Water:
Diluent;

ssw

InitiatonDate: "S ' jO* 2-

Termination Date





Code; PEG-01

Job:

22218

ssw

Sample Description;

Species:

Mcnidia beryllina

Code:

SS

Test Vessel;
Test Volume;

l-L Glass Jar

Product: Bry an Mound Oil WAF (25 gm/L)
Test: Effect Concentration (EC)

ID#;

2-13%

Age: ' Z<$

p Lafi^ig Com®

200-mLs per replicate

Sample
Description

%
WAF

R

E
p

A
B

C

A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Live Counts

pH

Dissolved Oxygen (rag/L)

(acceotsble minimum for a valid test is 4.0-ma/LA

Salinity (%o)

O0±25ic FalRe«/Vo!m.Nol4/Jan20lS>

V

Kr

•p-

fc.



0

24

48

72

96

0
new

24

old solution

48

old solution

71

old solution

96
old

0
new

24

old solution

48

old solution

72

old solution

96

old

0
new

24

old solution

old solution

72

old solution

96
old

Control

0

10

yo

it?

10

f Q>





2.0

x.l

5.)

^4



IS

7,3

>

Id}-



20,1

2-0



10

vo

(0

(0

So

10



iO

10

l&

WAF

6.25

10

w

tO

[0

to



S;o

wjHt

vz

*<>



3s

lb

73 .



¦Z-y)

2o.°l

1-6 J

20 J

I'D

10

\U>

(0

10

fo

10

|,u

(0

10

Vo

12.5

10



\0

to

(o

&.X

y>



lz

& 1



a.s



IX

15*



lX)g

20 J

zi. )



10

to

\Q

[0

la

10

"yo

10

10

IO

25

10



10

so

\ 1

20,7



10

VO

10

IO

i-o

10

yo

(0

It)

lo

100

10



c1

c

u

p*

s.o



U



1-7,



1, ^

7'1



it),®





2Ut>'

"4^

10



7

7



10

7*

7

7



Meter ID #:

X

X

436

X

A

tfsio





436





b

H?6

qzu



^3U

436

<436

03« Fit

Initials;

¦ffvv

wu,

P/*>

0/^





RAO

p/v0







IVUL.



0^





Ivu^

£>/K



Time:

iim



ISlO

U'#l5



Notes & Comments



Sample
Description

%
WAF

le

fMTO*

liiperature

anpp. for a valid le

°C)

«is?5±rQ ....



Control ID:

W 2-

fl jri

o

l!

£ HI
o' 8

& £

P 9

fU 1,0 P/n



0

24

4$

72

96

Diluent ID:





Control

0

I»3jfc

?-5>





tsg

Working Stock ID:

' SLN



WAF





z-r3



Oil ID ID;

22071 ¦chm



12.5

-re.*









Randomization
Template #

Feeding Type:

Artemia (concentrated slurry)



25



«

2 ?, 9

¦^-3

^•7

Amount:



none





50



s ?< L

"2-?^

If J

-? C.?

ib.1
left

>

Time:



X



Photoperiod is 16L:8D, Illuminiation is ambient (50 to 100 fled)
© Check Box: Lab ®» In-House Reared, Com = Commercially obtained





Meter ID #:

O*".

, h"LS

M"z4

(lis

Pl"5>

Version 2 (13040'DCF)	F:\Clients\PEQ-01, PegasusTechServ\22218,

! Bryan Mound WABBeneh&AnalyticaM} 22071-CHM (BryanMoundOil)\2) ECVPEG-01 22218,SS.96,ASRD.EFA2006.v2.RF(22018-CHM)

PEG-01 22218 revOl Page 26 of 44

-------
CETIS Analytical Report

Report Date:	10 Jul-23 13:39 (p 1 of 2)

Test Code/ID: PEG-01 22218SSA i 07-7018-8638

Reference Toxicant 96-h Acute Survival Test



Hydrosphere Research

Analysis ID;

11-2930-8763

Endpoint: f 96h Survival Rate}

CETIS Version:

CETISvl .9.7

Analyzed:

10 Jul-23 13:39

Analysis: Linear Interpolation (ICPIN)

Status Level:

1

Edit Date:

10 Jul-23 13:38

MD5 Hash: E37159E96EC4282E5B6BD6931244B90C

Editor ID:

003-737-857-6

Batch ID:

-03=2354-8760 .

f Start Date: 10 May-23 16:00
Ending Date: 14 May-23 15:50

Test Length; 96h

Protocol: EPA/821/R-02-012 (2002)
Species: t Menidia beryllina")

Taxon:

Actinopterygn

Analyst:
Diluent:
Brine:
Source:

Lab Tsch

Synthetic Saltwater

In-House Culture

Age:

Sample ID: 01-7230-7040

Code:

A453260

Project:

Product Toxicity Test

Sample Date: 08 Mar-23

Material:

Product

Source:

Pegasus Technical Services

Receipt Date: 08 Mar-23

CAS (PC):



Station:

| Bryan Mound Oil WAF (25gm/L) J

Sample Age: 63d 16h

Client:

Pegasus



		 "*•

Linear Interpolation Options
X T ransform Y T ransform

Seed

Resamples

Exp 95% CL Method

Log(X+1)

Linear

1733420 200

Yes

Two-Point interpolation

Test Acceptability Criteria	TAC L]mjts

Attribute	Test Stat Lower Upper

Overlap Decision

Control Resp

1

0.9

Yes

Passes Criteria

Point Estimates
Level mg/L

95% LCL 95% UCL

LC50 >100 J

96h Survival Rate Summary

Calculated Variate(A/B}

Isotonic Variate

Conc-mg/L j

Code

Count

Mean

Median

Min

Max

cv%

%Effect

A/B

Mean

%Effect

0 i

D

3

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1,0000

0,00%

6.25 |



3

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

30/30

1,0000

0.00%

12.5 |



3

1.0000

1,0000

1.0000

1.0000

0,00%

0.00%

30/30

1,0000

0.00%

25



3

0.9667

1.0000

0.9000

1.0000

5.97%

3,33%

29/30

0.9833

1.67%

50



3

10000

1.0000

1.0000

1.0000

0,00%

0.00%

30/30

. 0.9833

1,67%

100



3

0.6667

0.7000

0.6000

0.7000

8.66%

33.33%

20/30

0.6667

33.33%

96h Survival Rate Detail

Conc-mg/L Code

Rep 1

Rep 2

Rep 3

0 D

1.0000

1.0000

1.0000

6,25

1.0000 ,

1,0000

1.0000

12.5

1.0000

1.0000

1.0000

25

1.0000

1.0000

0.9000

50

1.0000

1.0000

1.0000

100

0,6000

0.7000

0.7000

96h Survival Rate Binomials







Conc-mg/L Code

Rep 1

Rep 2

Rep 3

0 D

10/10

10/10

10/10

6.25

10/10

10/10

10/10

12.5

10/10

10/10

10/10

25

10/10

10/10

9/10

50

10/10

10/10

10/10

100

6/10

7/10

7/10

003-737-857-6

CETIS™ v1.9.7.9

Analyst:	OA:

PEG-U1 Tim revO'l'Page 27 of 44

-------
CETIS Analytical Report	Report Date;	10 Jul-2313:39 (p 2 of 2)

Test Code/ID: PEG-01 22218SSA / 07-7018-8638

Reference Toxicant 96-h Acute Survival Test



Hydrosphere Research

Analysis ID;
Analyzed;
Edit Date:

11-2930-8763
10 Jul-23 13:39
10 Jul-23 13:38

Endpoint: 96h Survival Rate

Analysis: Linear Interpolation {ICPIN}

MDS Hash; E37159E96EC4282E5B6BD6931244B90C

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Graphics

003-737-857-6

CETIS™ v1.9.7.9

Analvst:	QA:

PEG-01 22218 revOl Page 28 of 44

-------
Hydrosphere

research

Client:

Pegasus Technical Services

Laboratory Notes
Code:

PEG-01

Job:

22218

Task Title:

25 gm/L WAF prep

Task Page 1 of 2

1) Product Name:

Bryan Mound Oil

Tech Initials:

PRM

2) Lab ID:

22071

CHM

Date: 5/9/2023

OIL ~ WAF Preparation (Effective Concentration Test)

oil mass for WAF



6.25
12.5
25
50
100

Bioassay WAF (mLs)

Number of renewals
WAF for renewals (mL)
Oil ehem for WAF
BTEX for WAF
Total WAF (mL)
Rounded
Mass of Oil (gm)

Saltwater Tests

SS

600

MS

600

37.5
75
150
300
600

37.5
75
150
1800
300

1162.5

2362.5

Both
1200

75
150
300
600
1200

2325

1

2325

1000

so

3405

3500

3605

87.5

Glass Cylinder (OD)

7 [diameter (in)
7 [height (in)
hei^it (cm)

17.2

17.78

Inside dimensions (measured, notcaM)

:cm (width) Item 1
cm (hci^it) Ism 2
:L (volnne) [item 3

17.3

4.0

Oli^WAF

OIL ~ WAF Preparation (Effective Concentration Test)

Saltwater SS & MS

Item 4

Item5
Item 6
Item 7;
Item 8:
Item 9
Item 10'

WAF needed

3500 JmLs

Cylinder 4019.7

cm {from inside bottom, up)
em (from inside bottom, up)

WAF height 15,06

hei^it+20%
cover lit of
is wfcat%?

18.08
17.3

14.8

20% vortex 12.05

em (from inside bottom, up)
% (shoot for 20%!)

cm (from inside bottom, up)

OIL

ftom "Mass of Oil (gms)"

Item 11
Item 12
Item 13

OH needed 87.5
Diameter) 17.20
OB thickness

0.38

Labor Hours to perforin tasks on this page:

NA

Version 2(1319 VBCBJ>eg-oi , Peg2susTechServ^222iS, Biyan Moulld WAFBencfc&AaalytiraM) 22071-CHM (BiymMejmldCMO'a)

-------
Hydrosphere

research

Client:

Pegasus Technical Services

Laboratory Notes
Code;

PEG-01

Job:

22218

Task Title

25 gm/L WAF prep



Task Page 2 of 2

1) Product Name:

Bryan Mound Oil

Tech Initials:

PRM

2) Lab ID:

22071

• CHM Date:

5/9/2023

WAF Mixture

Oil Mass:

25

gms/L 1) Initiation 18-hours of Mixing on Stir Plate (Target

Oil ID:

22071

• CHM is to achieve a 20% vortox)

Water Volume:

3.50

L

Water ID:

5976



ssw

Date:

5/9/2023



Product (unit/L):

NA

C

J3

sr

gm

Time:

14:26

>*N

5?

PRM



Product ID:

NA

* CHM Product Target:

# VALUE!

S
9

w

gm



Event Mass (gms) Action Product added:



a

a

&

gm



A

Oil Needed

87.5

Tare Cup

Time:

NA

0^

NA



B

Add Oil to Cup ©

90,20

weigh

Vortex Height:

15



%



C

Left in Cup

2.50

weigh



D

Total Oil in Jar

87.70

B-C

2) Terminate Mixing (at 18-hours)

E

Oil needed

-0.20

A-D



F

Target for Cup

2.30

C+E

Date



5/10/2023



G

Oil added to Cup



weigh

Time



8:25

SI

PRM



H

Left in Cup



weigh

System Stable?



yes



I
J

Oil added to Jar

0.00

G-H



Total Oil in Jar

87.70

D+I

3) Collection of WAF (after 6-hours settling)

K

Oil needed

-0.20

A-(D+H)

Time:

14:30

5.

PRM



L

Target for Cup

-0.20

H+K

Solution ID:

23138

• SLN

M

Oil added to Cup



weigh



N

Left in Cup



weigh



0

Oil added to Jar

0.00

M-N



P

Total Oil in Jar

87.70

J+O



Q

Percent of Total

100.23%

{(D+l+O)/A)*10Q



Notes & Comments

© Cup is an aluminum foil (acetone rinsed) lined 5.5 oz Solo Cup or DM16 cup

Version 2 (13191*0fi®^PEG-Ol,PegssusTechSetv,a22I8,B(yanMouBEG-O122218.1)LN,|jg'gte^F^2^l^yQ^ pagg 3Q of 44

-------
Hydrosphere

research

Hydrosphere Research

11842 Research Circle, Alachua FL 32615
(386) 462-7889

PAGE

of

Project Name:
Project Manager:

WAF Bioassays for Pegasus Technical Services

Peter R. Meyer

Company:
Address:

Sampler's Signature:

Hydrosphere Research

Phone: 386-462-7889

1Research Circle, Alachua FL 32615

llgA2 I





Sample I.D.

( Type / PrepDate/ WaterType I TestTy

Date

5/10/2023

Time

LAB ID

Matris

Is

.3 0*

S*

ja H

u h

as H
O

E
«

REMARKS

Field Reagent Blank (FRB)

15:00

TJPW(0.055|iS)

(BryanMoundOi I ¦ W AF/230510/Salt/AcuteEC, Control, @0hr/SS&MS

|BryanMouiidOil-WAF/230510/SaH/AcuteHC,6.2S%,@0hr/SS&MS
lBryanMoundOilWAF/230510/Salt/AcuteEC,10Q%,@0hr/SS&MS

5/10/2023

15:00

5/11/2023

15:00

5/10/2023

15:15

lprymrTiJniin^Oil-WAF/230512/Salt/AcuteEC,Control, @48hr/MS

| BryanMoundOi!-WAF/230512/Salt/AcuteEC,6.25%,@48hr/MS

|BryanMoundOil-WAF/230512/Salt/AcateEC,10Q%,@48hr/MS

iBrvanMoundOil-WAF/230514/Salt/AcuteEC,Control,@96hr/SS

lBivatiMoundOil-WAF/230514/Salt/AcuteEC,6.25%,@96hT/SS

iBrvanMoundOil- W AF/230514/Salt/AcuteEC,) 00% .@9 6hr/SS

5/12/2023

15:45

5/12/2023

15:45

5/12/2023

15:45

5/14/2023

16:00

5/14/2023

16:00

5/14/2023

116:00

SSW-5982

23139-SLN

23138-SLN

23143-SLN
23144'SLN

23145-SLN

23146-SLN

23147-SLN

23148-SLN

Grab

Grab

Grab

Grab

Grab

Grab

Grab

Grab

Grab

Grab

Correction: date is
5/10/2023, not
5/11/2023.
PRM-230710

(turnaround requirements

24 hr 	48 hr 	5 day

„ Standard (21 days)

Provide FAX Preliminary Results
^Requested Report Date:	

jlnvoice Information

1P.O.#		

IbUI to:	

REPORT REQUIREMENTS

I. Routine Report: Results, Method Blank,

Surrogate, as required
IL Report Diip., MS, MSD as required
HI. Data Validation Report (includes
raw data)

	IV. CLP Deliverable Report

V. EDD

Comments/Special Instructions:

SS = At beryliirta, MS = Mysidopsis bahia

RELIN

lsignature

[printed Name: Peter R. Meyer
lFim: Hydrosphere Research
Date/Time: 5->5'"^ )UJj7P

RECEIVED BY:
Signature:

Printed Name:
Finn: 	

Date/Time:

Printed Name:
Firm: 		

Date/Time:

Printed Name:
Firm: 	

Date/Time:

PEG-01 22218 revOl Page 31 of 44

-------
Appendix D.

Reference Toxicant Data for All Test Species

PEG-01 22218 revOl Page 32 of 44

-------
900

800

700

n

'M

o

600

"P
era

500

n

c

+

©'
s

400

300

200

100

-~-LC50
	AVG

—~—Upper Control Limit
—X—Lower Control Limit

I Hydrosphere

research

1000

Control Chart-I

Control Limits for Standard Reference Toxicant Tests
ACUTE ••• Mysidopsis bahia (cultured at Hydrosphere Research)

LC50 =	494 ug/L

The LC50 is within the control limits.

Note: Dates with no correspondindLCpO data point, if present on chart, indicates an invalid test.
Note: If the control limit(s) for two Br more consecutive tests are exceeded then the results must be
explained here and the test must be repeated immediately. (EPA-821-R-02-012, Sections 4.15.3
&4.15.4, page 18). Also, Section 4.15.2 of the same Method states that "...reference toxicant results
should not be used as a de facto criterion for rejection of individual effluent or receiving water tests."

Date: 06/02/2023

Test Dates

PEG-01 22218 revOl Page 33 of 44

-------
i Hydrosphere

research

0.500

0.450

0.400

0.350

0.300

0.250

0.200

0.150

0.100

0.050

0.000

Control Chart -II

Coefficient of Variation for Standard Reference Toxicant Tests
ACUTE ••• Mysidopsis bahia (cultured at Hydrosphere Research)

CV= 0.19

The CV is less than or equal to the national 90th percentile.
Comments (if needed):

¦CV

¦National 90th %



V?



/













o->





v

%

%

o

/



v?



"h
%







Test Dates

PEG-01 22218 revOl Page 34 of 44

-------
N

437

438

439

440

441

442

443

444

445

446

447

448

449

450

451

452

453

454

455

456

REFERENCE TOXICANT LOG • Last 20

Test: 48-hour Acute
Species: Mysidopsis bahia
Vendor: Hydrosphere Research

Toxicant: Copper Sulfate (fig Cu / L)

DATE

LC50

AVG

S.D.

2 SD

+ 2SD.

-2 SD

CV

National
75th %

National
90th %

Lower
Control
Limit

Upper
Control
Limit

9/8/2021

379

345.69

81.66

163.32

509.01

182.37

0.24

0.26

0.26

182.37

509.01

10/6/2021

402

346.03

81.89

163.78

509.81

182.25

0.24

0.26

0.26

182.25

509.81

10/28/2021

385

348.61

82.29

164.57

513.18

184.03

0.24

0.26

0.26

184.03

513.18

12/8/2021

462

358.63

83.33

166.66

525.28

191.97

0.23

0.26

0.26

191.97

525.28

1/5/2022

446

363.45

85.56

171.12

534.57

192.33

0.24

0.26

0.26

192.33

534.57

2/3/2022

472

364.17

86.44

172.88

537.05

191.28

0.24

0.26

0.26

191.28

537.05

3/2/2022

601

379.22

99.85

199.69

578.91

179.52

0.26

0.26

0.26

179.52

578.91

4/6/2022

472

388.70

99.13

198.26

586.95

190.44

0.26

0.26

0.26

190.44

586.95

5/4/2022

472

396.73

99.01

198.02

594.74

198.71

0.25

0.26

0.26

198.71

594.74

6/1/2022

398

403.09

94.48

188.96

592.05

214.12

0.23

0.26

0.26

214.12

592.05

7/6/2022

428

410.58

89.90

179.79

590.37

230.79

0.22

0.26

0.26

230.79

590.37

8/3/2022

290

410.61

89.86

179.72

590.33

230.88

0.22

0.26

0.26

230.88

590.33

9/8/2022

357

408.65

90.61

181.21

589.86

227.43

0.22

0.26

0.26

227.43

589.86

10/5/2022

322

404.18

92.64

185.28

589.46

218.89

0.23

0.26

0.26

218.89

589.46

12/7/2022

554

416.87

95.05

190.10

606.97

226.77

0.23

0.26

0.26

226.77

606.97

1/4/2023

513

423.17

97.14

194.28

617.45

228.89

0.23

0.26

0.26

228.89

617.45

2/1/2023

440

432.54

88.48

176.97

609.50

255.57

0.20

0.26

0.26

255.57

609.50

3/1/2023

323

436.51

81.05

162.10

598.60

274.41

0.19

0.26

0.26

274.41

598.60

4/5/2023

321

428.56

84.28

168.57

597.13

259.99

0.20

0.26

0.26

259.99

597.13

5/3/2023

494

426.61

82.16

164.32

590.93

262.28

0.19

0.26

0.26

262.28

590.93

PEG-01 22218 revOl Page 35 of 44

-------
B i o a

s s a y

) Hydrosphere

research

SRT
Toxicant

Standard Reference Toxicant Test

Job# | May 2023

Copper; Gu , desiccated..

Benehshcet Set § for this Job

(Unique rombtnafl/inerSiaple-f M«hod>

I]

Stock Solution

100 mg/L

Test Concentration

Hg/L

*

~

Document
Species
Feeding
Control Water

Acute Saltwater Method (El'A-821 -R-02-012)

Mysidopsis bahia

Code

• MS

Method#

2007.0

SOP#

TST-003

Artemis Nauplii; Concentrate

Rate Fed

2-drops (0.1-mL) 2-times, daily

SSW

Synihstie Seawater

SC 200 mLs

Test Vessel
Type

Plastic Cup; DM16

Day 0 ~ Start Date C, / ^	[day Note: Test Salinity is 20X. lit

[j^_fCoppert400l"Ls w/ SSWfl NA

0.22 1 0.44 . 0.88

1.76

3.52 Note: CASCADE is 7.04ml.s f 800mLs !

Dilution #
Toxicant (jig/L)

1

Control I Diluent ID [

pH (S.I.)

Dissolved Oxygen (nig.'"L )

Salinity (Xo)

Control

7,^

lA-

Xo»5

55

ML

'V>

mt

110





220



17

7.0^

440

x£

4a

zm

880

%.o

9..1



Toxicant ID

liof?

•SLN

pH Meter ID

D.O. Meter ID

Cond. Meter ID

<1l<

Live Counts; Replicate A

10

10

10

10

1Q

10

WQ Inititals



Live Counts; Replicate B

10

10

10

10

10

10

WQ Time

IZ

Stocking Initials

0/*s

Time |£ : Age ID#

713?

Randomization #

1

Feedings (Time)

Evening 2-drops (0.1-mL) 2-times, daily

Day 1 (24 hours) ~ Date \ $ / ^ / ~l*"h



day

Dilution# 1 2 3 4 5 6 Control/Diuent ID

NA

% Sample

Control

55

110

220

440

880

Effluent ID

NA

q Temperature (°C)

"H.I

7$,\

X\,D

xs.o





Thermometer ED

qzi

o pH (S.I.)

l,i

7.1

n,s

7,1

7.1

"7't

pH Meter ED

CIV

o

£ Dissolved Oxygen (mg/L)

2,5

M

%i

%*t





D.O. Meter ID

—J—

o 			-		

v> Salinity (%o)

?'¦*

7,t.6

ZM

7,1. £

Zi, ?



Cond. Meter ID

4/

Live Counts; Replicate A

10

l<3

1 o

ID

fo

V

Count &WQ Inititals



	

Live Counts; Replicate B

10 '

)Q

ft?

fO

(O

)0

Count &WQ Time

\\ -19

Feedings (Time)



Morning

U :¥J

Evening 2-drops (0.1-mL) 2-times, daily



Day 2 (48 hours) ~ End Date

S / $ / n

P

day Note: terminate test ±30 minutes of time initiated !!!

Dilution # 1 2 3 4 5 6



Control / Diluent ID

NA

% Sample

Control

55

no

220

440

880



Effluent ID

NA

n Temperature (DC)

-i C-V

1TJ • 1

—h9r^

7.V.7





\

^2) Thermometer ID

qtb

r	xssf

o pH(S.L)

7. *7

-7,1

7, 1

7, 7

7, 7

\



pH Meter ID

<1^1

o

g. Dissolved Oxygen (mg/L)



2,0



%o

a,o

\



D.O. Meter ID

|

o —	—						

8 Salinity (%.)

23,0

22°

W Count &WQ Time

1? m

Feedings (Time)

M:cC

Morning

—c>+\ $/$-•	

2-drops (0.1-mL) 24imes, daily

Notes & Comments

d) 12:00 m	&M 7,av. ?	^3"

?-/=/ 7

7.t

7, ?

VJ

2? t H"

Version 3 (22021 -DCF)	I	F«al<:hsbetts, Logs & fcimsM) BmdiSheetsW) 2022\200m AnulEMS(2022)\MS.4g.ASND.EPA2000.v3{22021-DCF)-S8.T

PEG-01 22218 revOl Page 36 of 44

-------
CETIS Analytical Report



Report Date:
Test Code/ID:

11 May-23 10:38 (p 1 of 2)
MAY23 MSA2 / 11-1922-4565

Mysidopsis

96-h Acute Survival Test



Hydrosphere Research

Analysis ID:
Analyzed:
Edit Date:

18-5334-1020
11 May-23 10:37
11 May-23 10:37

,/Endpoint: |48h Survival Rate |

Analysis: Linear Interpolation (1CPIN)

MD5 Hash: 9B2A474FF6E2D34D2D5E846A9C7135DE

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Batch ID: 03-2515-4518	Test Type: Survival (48h)

Start Date: 03 May-23 16:15"^	Protocol: EPA/821/R-02-012 (2002)

Ending Date: 05 May-23 15:45	Species: JjM^sicjopsis^bahia^

Test Length: 47h	Taxon: Malacostraca

Analyst:	Lab Tech

Diluent:	Synthetic Saltwater

Brine:	Tropic Marin

Source:	In-House Culture

Age:

Sample ID: 02-8866-8015
Sample Date: 03 May-23 16:15^/
Receipt Date: 03 May-23 16:15 *

Code:

1134B96F



Project:

Standard Reference Toxicant Test

Material:

Copper sulfate |

/

/source:

Reference Toxicant

CAS (PC):





Station:

Aquatic Indicators

Sample Age: —

Client:

Internal Lab '







Linear Interpolation Options











X Transform Y Transform

Seed

Resamples

Exp 95% CL

Method



Log(X+1) Linear

61400

200

Yes

Two-Point Interpolation



Point Estimates
Level pg/L

/9S%LCL 95% liCL

LC50 493.9 <*

493.9

493.9

48h Survival Rate Summary

Calculated Variate(AZB)

Isotonic Variate

,Conc-|jg/L

Code

Count -

^ Mean

Median

Min

Max

cv%

%Effect

A/B

Mean

%Effect

0

D

2

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

20/20

1.0000

0.00%

55



2

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

20/20

1.0000

0.00%

110



2

1.0000

1.0000

1.0000

1.0000

0,00%

0.00%

20/20

1.0000

0.00%

220



2

0.9500

0.9500

0.9000

1.0000

7.44%

5.00%

19/20

0.9500

5.00%

440



2

0.6000

0.6000

0.6000

0.6000

0.00%

40.00%

12/20

0.6000

40.00%

880



2

0.0000

0.0000

0.0000

0,0000

—

100.00%

0/20

0.0000

100.00%

48h Survival Rate Detail
^Conc-ug/L	Code

Rep 1

Rep 2

0

D

1.0000

1.0000

55



1.0000

1.0000

110



1,0000

1.0000

220



1.0000

0.9000

440



0.6000

0.6000

880



0.0000

0.0000

48h Survival Rate Binomials

Rep 1 ^

Rep 2 S

Conc-pg/L

Code

0

D

10/10

10/10

55



10/10

10/10

110



10/10

10/10

220



10/10

9/10

440



6/10

6/10

880



0/10

0/10

003-737-857-6

CETIS™ v1.9.7.9

Analyst:	 QA:_

PEG-01 22218 revOl Page 37 of 44

-------
CETIS Analytical Report

Report Date:
Test Code/ID:

11 May-23 10:38 (p 2 of 2)
MAY23 MSA2 /11-1922-4565

Mysidopsis 96-h Acute Survival Test



Hydrosphere Research

Analysis ID: 18-5334-1020 End point: 48h Survival Rate

Analyzed: 11 May-2310:37 Analysis; Linear Interpolation (ICPIN)

Edit Date: 11 May-23 10:37 MDS Hash: 9B2A474FF6E2D34D2D5E846A9C7135DE

CETIS Version:
Status Level:
Editor ID:

CETISvl .9,7
1

003-737-857-6

Graphics

003-737-857-6

CETIS™ v1.9.7.9	Analyst	 QA:_

JL

PEG-01 22218 revOl Page 38 of 44

-------
^QUATJc

r INDICATORS

Control Chart-I

Control Limits for Standard Reference Toxicant Tests
ACUTE ••• Menidia beryllina (conducted by Hydrosphere Research)

500

400

n

'j\

o

•p
era

O
c

o
s

300

200

100

^CU

LC50 = 208 Jig/L

Tlie LC50 is within the control limits.

%

QA Signature: f'

Date:

06/0C/2023

Note: Dates with no corresponding data point, if present on chart, indicates an invalid test.
Note: If the control limit(s) for two or more consecutive tests are exceeded then the results must be
explained here and the test must be repeated immediately. (EPA-821-R-02-012, Sections 4.15.3





03



*3

V

	AVG

—+—Upper Control Limit
—X—Lower Control Limit
-~-LC50

-X	X'



%

o

/



o



v>













V"

%





<3

v>

%

<5,

-------
AQUATIC

"INDICATORS

Control Chart -II

Coefficient of Variation for Standard Reference Toxicant Tests
ACUTE ••• Menidia beryllina (conducted by Hydrosphere Research)



0.60



0.58



0.55



0.53



0.50



0.48



0.45



0.43



0.40

n

0.38

o



fT>

a?

0.35

EE



r>*

0.33

rS*



3

0.30

o



«s

0.28



15

0.25

5'



rt-

0.23

o"



s

0.20

n

0.18

<







0.15



0.13



0.10



0.08



0.05



0.03



0.00

0.22

CY =	

The CV is less than or equal to the national 90th percentile.

Comments (if needed):

QA Signature: / \

Date

.06/07/2023

¦CV

¦National 75th %
¦National 90th %

% \	\ ^<9 ^ ^ ^ ^	%>

*0	<2> Q	Q	
-------
N

458

459

460

461

462

463

464

465

466

467

468

469

470

471

472

473

474

475

476

478

REFERENCE TOXICANT LOG • Last 20

Test: 48-hour Acute
Species: Menidict beryllina
Vendor: Aquatic Indicators
Toxicant: Copper Sulfate (fig Cu / liter)

Aquatic Indicators, Inc.

DATE

LC50

AVG

S.D.

2S.D.

+2S.D.

-2 SD

CV

National
75th %

National
90th %

Lower
Control
Limit

Upper
Control
Limit

10/28/2021

190

182

27.44

54.89

236.41

126.63

0.15

0.21

0.44

126.63

236.41

12/8/2021

270

185

33.82

67.64

253.10

117.82

0.18

0.21

0.44

117.82

253.10

1/5/2022

252

188

36.69

73.38

261.04

114.27

0.20

0.21

0.44

114.27

261.04

2/3/2022

202

188

36.83

73.66

261.87

114.55

0.20

0.21

0.44

114.55

261.87

3/2/2022

185

190

35.21

70.43

260.77

119.92

0.18

0.21

0.44

119.92

260.77

4/6/2022

198

191

35.06

70.12

261.61

121.37

0.18

0.21

0.44

121.37

261.61

5/4/2022

212

193

35.03

70.06

263.55

123.43

0.18

0.21

0.44

123.43

263.55

6/1/2022

156

192

35.98

71.97

264.02

120.09

0.19

0.21

0.44

120.09

264.02

7/6/2022

270

199

36.57

73.14

272.38

126.11

0.18

0.21

0.44

126.11

272.38

8/3/2022

290

204

41.81

83.62

287.48

120.23

0.21

0.21

0.44

120.23

287.48

9/8/2022

291

209

45.97

91.94

300.79

116.90

0.22

0.21

0.44

121.13

296.56

10/5/2022

211

209

45.96

91.93

301.07

117.22

0.22

0.21

0.44

121.30

296.98

11/9/2022

204

208

45.77

91.54

299.53

116.44

0.22

0.21

0.44

120.63

295.34

12/7/2022

189

210

43.97

87.93

297.87

122.00

0.21

0.21

0.44

122.00

297.87

12/22/2022

320

215

50.34

100.68

315.99

114.62

0.23

0.21

0.44

124.88

305.73

1/4/2023

300

224

69.52

139.04

362.69

84.62

0.31

0.21

0.44

129.72

317.59

2/3/2023

284

230

67.65

135.31

364.87

94.25

0.29

0.21

0.44

133.15

325.98

3/1/2023

160

227

51.79

103.58

330.88

123.72

0.23

0.21

0.44

131.84

322.77

4/5/2023

212

228

51.43

102.86

330.93

125.20

0.23

0.21

0.44

132.28

323.85

5/3/2023

208

230

49.52

99.04

329.25

131.16

0.22

0.21

0.44

133.52

326.90

PEG-01 22218 revOl Page 41 of 44

-------
Bio a

say

I f J >*¦

research

SRT
Toxicant

Standard Reference Toxicant Test	"| Job#	May 2023

Copper; Cu2+, desiccated

Benchstieet Set # for this Job

{Uniqse eeinMnathjii of Sample MeElmd)

Stock Solution lOOmg/L Test Concentration jig/L setw 1

of 1

Document
Species
Feeding
Control Water

Acute Saltwater Method (F.PA-K21 -R-02-012)

Menidia beryllina Code SS

Method # 2006.0

SOP#

TST-004

Anemia Nauplii; Concentrate | Rate Fed

0.2 mLs; 2-hours prior to test solution renewal

Exposure oAA mI e- lest Vessel
SSW Synthetic Seawater Volume TyPe

Plastic Cup; DM32

DayO Start Date | <; / V 111 w day Note: Test Salinity is 20%, HI

mLs<

5f 70

Toxicant (ng/L)

Control

75

150

300

600

1,200

Toxicant ID 'SLN

pH (S.I.)

7,?

t 0



1.°

9,°

g,0

pH Meter ID



-1

o, >g			

S M Dissolved Oxygen (mg/L)



t.n



iU

%.b

%¦ 1

D.O. Meter ID





n S 		

" Salinity (%o)

70,$

10$



zo £

20,4

10^

Cond. Meter ID

«

~

Live Counts; Replicate A

10

10

10

10

10

10

WQ Inititals

P/n

Live Counts; Replicate B

10

10

10

10

10

10

WQ Time

It:)j

Stocking Initials
Day 1 (24 hours) ~ Date

iVt

Time 1550 Agc l^Orr ro#

/ ^ |day



Randomization #

I

Dilution# 1 2 3 4 5 6 - Control / Diluent ID

NA

% Sample

Control

75

150

300

600

1200

Effluent ID

NA

n Temperature (°C)

-v> 



1,1

7( 1

-7.

7. ?

pH Meter ID

<|1(

	1

C£_

£ Dissolved Oxygen (mg/L)

7,9

Id

7.7

'1, 6

"7,7

7. X

D.O. Meter ID

.4-

a		

«> Salinity (%o)

Zl.V

us

zl,C

xi, i

"Z/,*

Z'.V

Cond. Meter ID



Live Counts; Replicate A

(0

I o

i o

3 7

1"

0'°

Count &WQJnititals

Pm

	

Live Counts; Replicate B

1 O

f 0

(O

He

3'

0'°

Count &WQ Time

11









Note: terminate test ±30 minutes of time initiated !!!

Day 2 (48 hours) - End Date

S7 S /?3

p

day

Dilution # 1 2 3 4 5 6 Control / D.luent ID

NA

% Sample

Control

75

150

300

600

1200

Effluent ID

NA

q Temperature (°C)







M,0

IS. |

\

Thermometer ID

VI*

O pH (S.I.)

7, &

n.%



1-



\

pH Meter ID

-------
CETIS Analytical Report

Report Date:
Test Code/ID:

11 May-23 10:29 (p 1 of 2)
MAY23 SSA / 09-6183-3342

Inland Silverside 96-h Acute Survival Test



Hydrosphere Research

Analysis ID: 10-4185-1005
Analyzed; 11 May-23 10:28
Edit Date; 11 May-23 10:28

Endpoint; 48h Survival Rate f

Analysis: Linear Interpolation (ICPIN)

MD5 Hash: 965379A7651F65E1373BE05603FB5078

CETIS Version:
Status Level:
Editor ID:

CETISvl .9.7
1

003-737-857-6

Batch ID:
Start Date:

15-8077-9378
03 May-23 15:50 ~
Ending Date: 05 May-23 15:30 ^
Test Length: 48h

Test Type:	Survival (48h)

Protocol:	EPA/821/R-02-012 (2002)

Species:	Menidia beryliina J

Taxon:	Actinopterygii

Analyst:	Lab Tech

Diluent;	Synthetic Saltwater

Brine:	Tropic Marin

Source:	Aquatic Indicators, FL

Age:

Sample ID: 02-3335-8766	Code:

Sample Date: 03 May-23 15:50 S.	Material:

Receipt Date: 03 May-23 15:50	CAS (PC):

Sample Age: —	Client:

DE8C5AE	/

Copper sulfate

Aquatic Indicators

Project: Standard Reference Toxicant Test
Source: Reference Toxicant
Station: Aquatic Indicators

Linear Interpolation Options

Resamples Exp 95% CL Method

Log(X+1)

Linear

819946 200



Yes

Two-Point Interpolation







Point Estimates























Level pg/L

/ 95% LCL

95% UCL



















LC50 207.9

154.6

258.4



















48h Survival Rate Summary







Calculated Varlate(AZB)





isotonic Variate

/Conc-pg/L

Code

Count

Mean

Median

Min

Max

CV%

% Effect

A/B

Mean

%Effect

0

D

2

1.0000

1.0000

1.0000

1.0000

0.00%

0.00%

20/20

1.0000

0,00%

75



2

1,0000

1.0000

1.0000

1,0000

0.00%

0.00%

20/20

1.0000

0.00%

150



2

0.9000

0.9000

0.8000

1.0000

15.71%

10.00%

18/20

0.9000

10.00%

300



2

0.0500

0,0500

0.0000

0.1000

141.42%

95.00%

1/20

0.0500

95.00%

600



2

0.0000

0.0000

0.0000

0.0000

_

100.00%

0/20

0.0000

100.00%

1200



2

0.0000

0.0000

0.0000

0.0000

...

100.00%

0/20

0.0000

100.00%

48h Survival Rate Detail



Rep 1 Rep 2

0

D

1.0000

1.0000

75



1.0000

1.0000

150



1.0000

0.8000

300



0.1000

0,0000

600



0.0000

0.0000

1200



0.0000

0.0000

48h Survival Rate Binomials
Conc-pg/L	 Code Rep 1

/ Rep 2 ^

0

75

150

300

600

1200

D

10/10

10/10

10/10

1/10

0/10

0/10

10/10

10/10

8/10

0/10

0/10

0/10

003-737-857-6

CETIS™ vi .9.7.9

Analyst:	 QA._ L/tT

PEG-01 22218 revOl Page 43 of 44

-------
CETIS Analytical Report

Report Date:
Test Code/ID;

11 May-23 10:29 (p 2 of 2)
MAY23 SSA / 09-6183-3342

Inland Silverside 96-h Acute Survival Test



Hydrosphere Research

Analysis ID: 10^185-1005 Endpoint: 48h Survival Rate

Analyzed: 11 May-23 10:28 Analysis: Linear Interpolation (ICPIN)

Edit Date: 11 May-23 10:28 MD5 Hash: 965379A7651F65E1373BE05603FB5078

CETIS Version:
Status Level:
Editor ID:

CETlSvl .9.7
1

003-737-857-6

Graphics

1 vV

003-737-857-6	CETIS™ v1.9.7.9	Analyst	 QA:_VlL

PEG-01 22218 revOl Page 44 of 44

-------
APPENDIX 2

EPA/600/X-23/356

-------
Pegasus Technical Services, Inc.

Bryan Mound Crude Oil

September 2022

Job: 15911

•£v

Core Lab

tesiswis wrniKAiisw

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Digitally Generated by; Saybolt LP
Reason: Laboratory data
Location: Deer Park - Laboratory
Date: 2023.05.31 20:46:57 -06:00

Saybolt

A CJ.1I lABdBHSSUS CDKPAKJ

Attention of
Analysis Report
Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (Whole Oil)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

03-24-2023

05-31-2023

14490450

NAME

METHOD

UNIT

RESULT

Gravity API at 60 °F
Relative Density at 60/60 °F
Asphaltenes
Micro Carbon Residue
UOP K factor
Organic chloride
Organic chloride in Naphtha fraction proc. I
Organic chloride in original sample
Hydrogen Sulfide and Mercaptan Sulphur
Hydrogen Sulfide, as S
Mercaptan, as S
Hydrogen Sulfide
Kinematic Viscosity at 77 °F
Kinematic Viscosity at 100 °F
Metals by ICP
Copper (Cu) Method B
Iron (Fe) - Method B
Nickel (Ni) - Method B
Vanadium (V) - Method B
Nitrogen
Pour Point
Salt Content
Sediment By Extraction
Sulphur (S)

VPCR4 at 100 °F
Water Karl Fischer
Wax content
Acid Number
Acid number
Sample Size

ASTM D 5002
ASTM D 5002
ASTM D 6560
ASTM D 4530

UOP 375
ASTM D 4929

UOP 163

ASTM D 5705
ASTM D 445
ASTM D 445
ASTM D 5708

ASTM D 5762
ASTM D 97

ASTM D 3230
ASTM D 473
ASTM D 4294
ASTM D 6377
ASTM D 4928
UOP 46 obs.
ASTM D 664

"API

mass %
mass %

pg/g

pg/g

mg/kg

mg/kg

ppm

mm2/s

mm2/s

mg/kg
mg/kg
mg/kg
mg/kg
WT%

•c

WT%
mass %
mass %
psi

mass %
mass %

mg KOH/g

g

38.6

0.8320

0.48

2.11

12.1

<1
<1

<1

14,5

<1

4.721
3.547

<0.1
1.1
4.3
8.7

0.0832
-48

0.00038

0.03

0.377

6.54

0.02

3.6

Oidii
6 69

Ali results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis, Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Sayboit's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

Print Dale: 05-31-202315:46
Page 1 of 3

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (Whole Oil)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

03-24-2023

05-31-2023

14490450

NAME

METHOD

UNIT

RESULT

Inflection or buffer endpoint



-

Inflection

Light Hydrocarbons in Crude Oil, Extended

ASTM D 7900 mod.





Benzene



LV %

0.27

Toluene



LV %

0.60

Ethyl Benzene



LV %

0.14

M-Xylene



LV %

0.39

p-Xylene



LV %

0.12

o-Xylene



LV %

0.20

Simulated Distillation

ASTM D 7169





IBP (0.5%)



°F

21

5% recovered



°F

138

10% recovered



°F

193

15% recovered





236

20% recovered



°F

278

25% recovered



•F

327

30% recovered



°F

377

35% recovered



°F

428

40% recovered



°F

475

45% recovered



"F

520

50% recovered



°F

568

55% recovered



°F

614

60% recovered



°F

666

65% recovered



°F

720

70% recovered



°F

777

75% recovered



°F

830

80% recovered



°F

897

85% recovered



°F

978

90% recovered



°F

1085

95% recovered



°F

1242

FBP (99.5%)



•F

>1328

Boiling Range Residue



mass %

3.5

All results in this report refer to the sample(s) tested as taken or submitted like specified In this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AB tests are conducted according to the latest version of the methods, unless another version Is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with suppled specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory,

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any suppled specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Sayboit LP 201 Deerwood Glen Dr, Deer Park, IX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: sayboit.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available atwww.corelab.com/saybolt/te/ms-conditions

Print Date: 05-31-2023 15:46
Page 2 of 3

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

SayhulL

a cm uiwuiuin

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

03-24-2023

05-31-2023

14490450

Bryan Mound Crude Sep 2022 (Whole Oil)

NAME

METHOD

UNIT

RESULT

Recovery

mass %

96.5

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

All results in Itiis report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the lest results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated, Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation ot test results
as defined in AS1M D3244, IP 387. ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory,

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested paramefers against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Dale: 05-31-2023 15-46

Tel. +1 261 478 1300 Fax. +1 281 476 9638	Pago 3 nf 3

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Sayboll's terms md conditions, available at wwwxorelab.com/saylyollAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Say bait

Attention of
Analysis Report
Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (IBP-75°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybott LP, Deer Park

05-14-2023

05-14-2023

14586585

NAME

METHOD

UNIT

RESULT

Yield, LV%

ASTM D 2892

LV%

3.16

Yield, LV%

ASTM D 2892

LV %

2.23

Gravity API at 60 °F

ASTM D 4052

"API

111.7

Relative Density at 60/60 °F

ASTM D 4052

-

0.5818

DHA Analysis

DHA



Attached

Signed by: Dan Carlson - Chemist Ml
Issued by; Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically Indicated. Where available and for convenience purposes, the tested sample has
been checked for compMancewith suppBed specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367. ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the ongin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample Identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Dser Park, TX 77S36	Print Dais: 05-31-2023 15-46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Paga 1 of 1

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelobxom/sayboll/tsms-condillons

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybuit

Attention of

Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (75-175°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-05-2023
05-25-2023
14586586

NAME

METHOD

UNIT

RESULT

Yield, LV%

Yield on crude

Gravity API at 60 °F

Relative Density at 60/S0 °F

DHA Analysis

Sulphur (S)

Organic chloride

Hydrogen Sulfide and Mercaptan Sulphur
Hydrogen Sulfide, as S
Mercaptan, as S
Hydrogen Sulfide
Research Octane Number (RON)

Motor Octane Number (MON)

Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered
20% recovered
25% recovered
30% recovered
35% recovered
40% recovered
45% recovered
50% recovered
55% recovered
60% recovered
65% recovered
70% recovered
75% recovered

ASTM D 2892
ASTM D 2892
ASTM D 4052
ASTM D 4052
DHA

ASTM D 5453
ASTM D 4929
UOP 163

ASTM D 5705
ASTM D 2699
ASTM D 2700
ASTM D 2887

LV %
vol %
"API

Wt%
ppm

mg/kg
mg/kg
ppm

8.32

6.76

79.6

0.6702

Attached

0,0013

<1

<1
11
<1
67
65

28

70

74

90

92

94

96

98

113

132

135

140

148

157

158

159

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Whore available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as lo origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the iested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Or, Deer Park, TX 77536
Tel. +1 281 478 1300 Fax. +1 281 476 9638
Website: www.saybolt.com E-mail: saybolt.deerparklab@coreiab.com
All our activities are carried out under Sayboll's terms and conditions, available at»

Print Date: 05-31-2023 15:46
Page 1 of 2

w.corelab.com/sayboMerrns-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE	1

45268, Cincinnati

United States	_ . ..

Saybolt

A

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (75-175°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-05-2023
05-25-2023
14586586

NAME

METHOD UNIT

RESULT

80% recovered

•F

166

85% recovered

°F

169

90% recovered

°F

180

95% recovered

°F

187

FBP (99.5%)

'F

209

Boiling Range Residue

mass %

-

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

Al! results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the suppfied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Sayboit expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date: 05-31-2023 15 46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of2

Website: www.saybott.com E-mail: saybolt.deerparklab@corelab.com

Alt our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/saylioltAerms~conditions

-------
PEGASUS TECHNICAL SERVICES, INC.	~

26 WEST MARTIN LUTHER KING DRIVE	' |a

45268, Cincinnati

United States	*

Saybolt

A CSM U&ORMORItS C3HPAKV

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1 /L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (175-250T)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-05-2023

05-25-2023

14586587

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
PIANO

See Attached
Sulphur (S)

Organic chloride

Organic chloride in original sample
Hydrogen Sulfide and Mercaptan Sulphur
Hydrogen Sulfide, as S
Mercaptan, as S
Hydrogen Sulfide
Research Octane Number (RON)

Motor Octane Number (MON)

Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered
20% recovered
25% recovered
30% recovered
35% recovered
40% recovered
45% recovered
50% recovered
55% recovered
60% recovered

ASTM D 2892
ASTM D 2892
ASTM D 4052

ASTM D 6733

ASTM D 5453
ASTM D 4929

UOP 163

ASTM D 5705
ASTM D 2699
ASTM D 2700
ASTM D 2887

vol %
mass %

mg/kg

Mg/g

mg/kg
mg/kg
ppm v/v

•F

°F
°F
"F
°F
°F
•F
°F
"F
°F
°F
°F
•F

10.22
9.14

60.5
0.7371

See Attached
0.0018

<1

<1
10
8

55

56

132
167
185
191
196
201
205
211
213
215
221

223

224

All results in this report refer to the sampie(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any suppled specification for purposes of estabfishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboHAerms-conditions

Print Date: 05-31-2023 15:46
Page 1 of 2

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States	_ , .

Saybolt



A CD Fit UBBBATOItS CBR?A«»

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (175-250T)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-05-2023

05-25-2023

14586587

NAME

METHOD

UNIT

65% recovered
70% recovered
75% recovered
80% recovered
85% recovered
90% recovered
95% recovered
FBP (99.5%)

°F
°F
°F
°F
T
°F
"F
°F

RESULT

229
237
241
247
251
260
264
286

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

At) results in this report refer to the samp!e(s) tested as taken or submitted iike specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
Ah tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compfiance with supplied specifications, without accepting any liability for the supplied information, In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
irade solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any suppSed specification for purposes of establshing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date' 05-31-2023 1546

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 2

Website: www.sayboit.com E-mail: saybolt.deerparkiab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE	>

45268, Cincinnati	¦ - .

United States	7

Saybolt

h tost UIBHMiiRttS CDMFA.NY

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

: Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (250-375°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-05-2023
05-25-2023
14586588

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
PIANO

See Attached
Organic chloride

Organic chloride in original sample
Hydrogen Sulfide and Mercaptan Sulphur
Hydrogen Sulfide, as S
Mercaptan, as S
Hydrogen Sulfide
Research Octane Number (RON)

Motor Octane Number (MON)

Aniline Point
Elemental analysis
Carbon
Hydrogen
Naphthalenes

Naphthalenes procedure B
Smoke Point
Sulphur (S)

Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered
20% recovered
25% recovered

ASTM D 2892
ASTM D 2892
ASTM D 4052

ASTM D 6733
ASTM D 4929
UOP 163

ASTM D 5705
ASTM D 2699
ASTM D 2700
ASTM D 611
ASTM D 5291

ASTM D 1840

ASTM D 1322
ASTM D 4294
ASTM D 2887

vol %
mass %

M9/9

mg/kg
mg/kg
ppm v/v

mass %
mass %

vol %
mm
mass %

"F
°F
°F
°F
°F
°F

13.87
13.05

51.0
0.7755

See Attached

<1

<1

26.4

60

30

27

53.90

85.89
14.09

0.28
23

0.0117

210
247
261
273
278
286

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
Ait tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compfiance with suppled specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product Identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536
Tel. +1 281 478 1300 Fax. +1 281 476 9638
Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com
All our activities are carried out under Saybolt's terms and conditions, available at w

Print Date: 05-31-2023 15:46
Page 1 of 2

v.corelab.com/saybottAemS'Conditions

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States	_ , ,

Saybult

iv

A Cast lAEHRATDRILS CC

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (250-375°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-05-2023
05-25-2023
14586588

NAME

METHOD UNIT

RESULT

30% recovered

*F

290

35% recovered

°F

296

40% recovered

°F

305

45% recovered

°F

309

50% recovered

°F

314

55% recovered

°F

322

60% recovered

°F

328

65% recovered

°F

334

70% recovered

°F

340

75% recovered

°F

348

80% recovered

°F

351

85% recovered

°F

360

90% recovered

•F

370

95% recovered

°F

385

FBP (99.5%)

°F

406

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

Alt results in this report refer to the samplefs) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaiuation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied Information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This roport shall not be partially copied and reproduced without the written permission of the laboratory,

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysts. Saybolt has not verified the tested parameters against any suppled specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Gleri Dr, Deer Park, TX 77536	Print Date- 05-31-2023 15 46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 2

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Sayboit's terms and conditions, available at www.corelab.com/sayboltAerms-condltions

-------
PEGASUS TECHNICAL SERVICES, INC.	A

26 WEST MARTIN LUTHER KING DRIVE	.1 %

- &
Saybolt

45268, Cincinnati
United States

ACSRC USOHMURitS COHPAST

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (375-530°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-05-2023

05-31-2023

14586589

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
Organic chloride

Organic chloride in original sample
Hydrogen Sulfide and Mercaptan Sulphur
Hydrogen Sulfide, as S
Mercaptan, as S
Hydrogen Sulfide
Aniline Point
Elemental analysis
Carbon
Hydrogen
Naphthalenes

Naphthalenes procedure B
Smoke Point
Sulphur (S)

Acid Number
Acid number
Sample Size

Inflection or buffer endppoint
Cloud Point
Freezing Point
FIA (hydrocarbon types)

Saturates
Olefins
Aromatics
Kinematic Viscosity at 77 °F

ASTM D 2892
ASTM D 2892
ASTM D 4052

ASTM D 4929

UOP 163

ASTM D 5705
ASTM D 611
ASTM D 5291

ASTM D 1840

ASTM D 1322
ASTM D 4294
ASTM D 664

ASTM D 2500
ASTM D 2386
ASTM D 1319

ASTM D 445

vol %
mass %

M9/g

mgAg
mg/kg
ppm v/v
°C

mass %
mass %

vol %
mm

mass %
mg KOH/g

°C
°C

vol %
vol %
vol %
mm2/s

15.96
15.77

40.6
0.8221

<1
23
90

64.55

85.87
14.06

3.44
17

0.0646
0.1

20.0155
Inf
-39
-35.0

81.9
1.0
17.1
2.361

All results in this report refer to the sampiefs) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with suppled specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244. IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin Is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt IP 201 Deerwood Glen Dr, Doer Park, TX 77536
Tel. +1 281 478 1300 Fax. +1 281 476 9638
Website: www.sayboft.com E-mait: saybolt.deerparklab@corelab.com
All our activities are carried out under Seybolt's terms and conditions, available at w

Print Date: 05-31-2023 15:46
Page 1 of 3

w.corelab.com/sayboltAemts-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

A tSSt UI8R.<1l)R|[S COKfkNT

Attention of	: Ms. D. Sundaravadivelu

Analysis Report

Report number

13072/00015911.1/L/23

Submitted date

03-24-2023







Sample submitted at

Saybolt LP, Deer Park

Report Date

05-31-2023

Date received

05-05-2023



Date of issue

05-31-2023

Date completed

05-31-2023



Sample object

Pegasus Tech Svc

Sample number

14586589



Sample type

Submitted







Sample submitted as

Petroleum Crude Oil







Marked

Bryan Mound Crude Sep 2022 (375-530T)





NAME



METHOD

UNIT

RESULT

Kinematic Viscosity at 100 °F

ASTM D 445

mm2/s

1.885

Total Nitrogen



ASTM D 4629

mg/kg

1.6

Pour Point 1



ASTM D 97

"C

-41

UOP K factor



UOP 375

-

11.8

Cetane Index



ASTM D 976

-

47.8

Simulated Distillation



ASTM D 2887





All results in this report refer to the sample{s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compSance with suppfied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis, Saybolt has not verified the tested parameters against any supplied specification for purposes of establshing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample Identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	print Date- 05-31-2023 15 46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 3

Website: www.sayboit.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States	_ . _

Saybolt



A tOBt UEORMOIlllS [t

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (375-530°F)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-05-2023

05-31-2023

14586589

NAME

METHOD UNIT

RESULT

IBP (0.5%)

°F

333

5% recovered

°F

377

10% recovered

°F

389

15% recovered

°F

402

20% recovered

"F

413

25% recovered

°F

423

30% recovered

"F

428

35% recovered

°F

438

40% recovered

*F

448

45% recovered

°F

455

50% recovered

°F

461

55% recovered

•F

469

60% recovered

°F

479

65% recovered

°F

487

70% recovered

•F

493

75% recovered

°F

499

80% recovered

*F

509

85% recovered

°F

516

90% recovered

°F

524

95% recovered

"F

536

FBP (99.5%)

•F

571

Remarks:

1 Derived from Spiral

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

All results in this report refer to the sample(s} tested as taken or submitted like specified in this Anaiysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied Information. In case of dispute or concern, we refer to the Interpretation of test results
as defined in A SIM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the ongin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date: 05-31-2023 15:46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 3 of 3

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/saybolMerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

A [BRE UBBHATORltS CBHPAKY

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1 /L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (530-650T)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-05-2023

05-25-2023

14586590

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
Aniline Point
Elemental analysis
Carbon
Hydrogen
Naphthalenes

Naphthalenes procedure B
Smoke Point
Sulphur (S)

Acid Number
Acid number
Sample Size

Inflection or buffer endpoint
Cloud Point

Kinematic Viscosity at 130 °F
Kinematic Viscosity at 100 °F
Nitrogen
Pour Point
UOP K factor
Cetane Index
Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered
20% recovered

ASTM D 2892
ASTM D 2892
ASTM D 4052

ASTM D 611
ASTM D 5291

ASTM D 1840

ASTM D 1322
ASTM D 4294
ASTM D 664

ASTM D 2500
ASTM D 445
ASTM D 445
ASTM D 5762
ASTM D 97
UOP 375
ASTM D 976
ASTM D 2887

vol %
mass %

°C

mass %
mass %

vol %
mm

mass %
mg KOH/g

°C

mraVs

mm2/s

WT%

°C

°F
°F
°F
°F
"F

12.16
12.50

34.0

0.8549

76.70

86.53
13.22

7.83
15

0.241
0.2

20.0080

Inf

14

3.498
5.188
0.0163
-6

11.8
52.0

495
532
545
553
560

All results in this report refer to the samp)e(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for complance with suppSed specifications, without accepting any liability for the supplied Information. In case of dispute or concern, wo refer to the interpretation of test results
as defined In ASTM D3244, IP 367, (SO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any suppled specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deefparklab@corelab.com

AJI our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

Print Date: 05-31-2023 15:46
Page 1 of 2

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE

45268, Cincinnati
United States

Saybult

A Clf.1t i-ABOHATUKIfa CflflMNt

Attention of	: Ms. D. Sundaravadiveiu

Analysis Report

Report number

13072/00015911.1/L/23 Submitted date

03-24-2023





Sample submitted at

Saybolt LP, Deer Park

Report Date

05-31-2023 Date received

05-05-2023



Date of issue

05-31-2023 Date completed

05-25-2023



Sample object

Pegasus Tech Svc Sample number

14586590



Sample type

Submitted





Sample submitted as

Petroleum Crude Oil





Marked

Bryan Mound Crude Sep 2022 (530-650T)





NAME

METHOD

UNIT

RESULT

25% recovered



°F

568

30% recovered



°F

574

35% recovered



°F

581

40% recovered



°F

584

45% recovered



°F

590

50% recovered



°F

597

55% recovered



°F

605

60% recovered



°F

609

65% recovered



•F

615

70% recovered



°F

623

75% recovered



°F

630

80% recovered



°F

635

85% recovered



°F

644

90% recovered



°F

652

95% recovered



°F

660

FBP (99.5%)



°F

695

Signed by: Dan Carison - Chemist !ii
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

A" results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compSance with suppBed specifications, without accepting any liability for the supplied Information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any suppSed specification for purposes of estabfishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Or, Deer Park, TX 77536	Print Date' 05-31-2023 15 46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 2

Website: www.saybolt.com E-mail: sayboft.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States



J

Saybolt

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (650°F plus)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-07-2023
05-31-2023
14586591

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity '

Relative Density at 60/60°F 1
Acid Number
Acid number
Sample Size

Inflection or buffer Endpoint
Asphaltenes
Micro Carbon Residue
Kinematic Viscosity at 130 °F
Kinematic Viscosity at 180 °F
Metals by ICP
Iron (Fe) - Method B
Nickel (Ni) - Method B
Vanadium (V) - Method B
Metals by ICP

Copper (Cu) - Method B
Nitrogen
Pour Point
Sulphur (S)

Elemental analysis
Carbon
Hydrogen
Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered

ASTM D 2892
ASTM D 2892
ASTM D 4052

ASTM D 664

ASTM D 6560
ASTM D 4530
ASTM D 445
ASTM D 445
ASTM D 5708

ASTM D 5708

ASTM D 5762
ASTM D 97
ASTM D 4294
ASTM D 5291

ASTM D 7169

vol %
mass %

mg KOH/g
9

mass %
mass %
mmVs
mmVs

mg/kg
mg/kg
mg/kg

mg/kg
WT %

*C

mass %

mass %
mass %

°F
°F
•F
°F

36.31
40.55

20.8
0.9291

0.58

1.3233

Inf

1.2

4.96

102.4

31.66

0.4

10.2

19.2

<0.1

0.2076

24

0.824

86.72
12.26

639
675
699
723

Ail results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted accorcfing to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compfiance with suppled specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of estabfishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.sayboit.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.cow/saybolt/terms-conditions

Print Date: 05-31-2023 15:46
Page 1 of 2

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States	_ , ,

Say bull

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/U23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (650°F plus)

Submitted date
Sample submitted at
Date received
Date completed
Sample number

04-16-2023
Saybolt LP, Deer Park

05-07-2023
05-31-2023
14586591

NAME

METHOD UNIT

RESULT

20% recovered

°F

748

25% recovered

°F

772

30% recovered

°F

794

35% recovered

•F

818

40% recovered

°F

843

45% recovered



871

50% recovered

*F

900

55% recovered

•F

932

60% recovered

°F

966

65% recovered

*F

1005

70% recovered

°F

1048

75% recovered

°F

1096

80% recovered

°F

1152

85% recovered

°F

1216

90% recovered

°F

1298

95% recovered

°F

-

FBP (99.5%)

°F

>1328

Boiling Range Residue

mass %

8.1

Recovery

mass %

91.9

Remarks:
1 by Calc.

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

A!! results in this report refer to the sample(s) tested as taken or submitted iike specified in this Analysis report. Uncertainties, available on request, apply In the evaluation of the test results.
AB tests are conducted according to the latest version of the methods, unless another version is specifically Indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the Interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided Is solely as to the physical and chemical properties of the sample Identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date- 05-31-2023 15 46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 2

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activiiies are carried out under Sayholt's terms and conditions, available at www.corelab.com/saybolt/1erms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States

&

Saybult

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (650-850T )

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586592

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
Aniline Point
Elemental analysis
Carbon
Hydrogen
Sulphur (S)

Acid Number
Acid number
Sample Size

inflection or buffer endpoint
Cloud Point 1

Kinematic Viscosity at 130 °F
Kinematic Viscosity at 180 °F
Nitrogen
Pour Point

Micro Carbon Residue
Refractive index at 60 °C
Cetane Index 1
Wax content
UOP K factor
Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered
20% recovered

ASTM D 5236
ASTM D 5236
ASTM D 4052

ASTM D 611
ASTM D 5291

ASTM D 4294
ASTM D 664

ASTM D 2500
ASTM D 445
ASTM D 445
ASTM D 5762
ASTM D 97
ASTM D 4530
ASTM D 1747
ASTM D 976
UOP 46 obs.
UOP 375
ASTM D 2887

vol %
mass %

"C

mass %
mass %
mass %

mg KOH/g
9

"C

mmVs
mm2/s
WT%

°C

mass %

mass %

°F
°F
°F
°F
"F

15.60
16.77

26.7

0.8947

87.65

86.53
12.94
0.464

0.38

4.0534

Inf

28.6

14.72

6.736

0.0684

24

<0.01

I.4795
61.4
13.1

II.9

639
672
684
695
705

Ail results in this report refer to the sampie(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
Afl tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compiance with suppfied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample fur analysis. Saybolt has not verified the tested parameters against any suppled specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboitAorms-conditions

Print Date: 05-31-2023 15:46
Page 1 of 2

-------
PEGASUS TECHNICAL SERVICES, INC.

26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati

United States	_ , ,

Saybolt



A CBSE UEOKATtjmtS tUMFAKT

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (650-850T

Submitted date
Sample submitted at
Date received
Date completed
Sample number

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586592

NAME			METHOD	UNIT	RESULT

25% recovered

°F

715

30% recovered

°F

724

35% recovered

°F

735

40% recovered

*F

745

45% recovered

°F

756

50% recovered

°F

766

55% recovered

°F

777

60% recovered

°F

788

65% recovered

°F

799

70% recovered

°F

811

75% recovered

°F

824

80% recovered

•F

837

85% recovered

°F

852

90% recovered

°F

872

95% recovered

°F

902

FBP (99.5%)

°F

970

Remarks:

1 Derived from Spiral

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

Al! results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AH tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date- 05-31-2023 15-46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	page 2 of 2

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

AS our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms, D. Sundaravadivelu

13072/00015911.1/L/23

Submitted date
Sample submitted at
Date received
Date completed
Sample number

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (850-1050°F )

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586593

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity

Relative Density at 60/60°F
Aniline Point
Elemental analysis
Carbon
Hydrogen
Sulphur (S)

Acid Number
Acid number
Sample Size

Inflection or buffer endpoint
Cloud Point 1

Kinematic Viscosity at 130 °F
Kinematic Viscosity at 180 °F
Nitrogen
Pour Point

Micro Carbon Residue
Refractive index at 60 °C
Wax content
Metals by ICP
Iron (Fe) - Method B
Nickel (Ni) - Method B
Vanadium (V) - Method B
Metals by ICP

Copper (Cu) - Method B
UOP K factor
Simulated Distillation

ASTM D 5236
ASTM D 5236
ASTM D 4052

ASTM D 611
ASTM D 5291

ASTM D 4294
ASTM D 664

ASTM D 2500
ASTM D 445
ASTM D 445
ASTM D 5762
ASTM D 97
ASTM D 4530
ASTM D 1747
UOP 46 obs.
ASTM D 5708

ASTM D 5708

UOP 375
ASTM D 7169

vol %
mass %

mass %
mass %
mass %

mg KOH/g
9

•C

mmVs

mm2/s

WT%

"C

mass %

mass %

mg/kg
mg/kg
mg/kg

mg/kg

11.52
12.76

22.0
0.9216
100.40

86.63
12.55
0.668

0.59

2.0362

Inf

46.6

87.68

26.90

0.1522

45

0.90

1.4955

14.9

<0.1

0.1

0.1

<0.1

12.1

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AN tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of estabfishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

Print Date: 05-31-2023 15:46
Page 1 of 3

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D, Sundaravadivelu

13072/00015911.1/U23

Submitted date
Sample submitted at
Date received
Date completed
Sample number

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (850-1050°F }

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586593

NAME

METHOD

UNIT

RESULT

IBP (0.5%)
5% recovered
10% recovered
15% recovered
20% recovered
25% recovered
30% recovered
35% recovered
40% recovered
45% recovered
50% recovered
55% recovered
60% recovered
65% recovered
70% recovered
75% recovered
80% recovered
85% recovered
90% recovered
95% recovered
FBP (99.5%)

Boiling Range Residue
Recovery

°F
°F
•F
°F
°F
°F
*F
°F
°F
°F
°F
°F
°F
°F
°F
•F
°F
•F
°F
°F
•F

mass %
mass %

692

780

813

836

854

869

883

896

908

919

930

940

951

963

975

987

1000

1014

1032

1054

1122

0.5

99.5

Remarks:

1 Deerived from Spiral

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31 -2023

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
Alt tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of estabfishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date* 05-31-2023 15-46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 3

Website: www.sayboft.com E-mail: saybolt.deerparklab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.coielab.com/saybott/terms-conditions

-------
All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
AB tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compfiance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in AS7M D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date- 05-31-2023 1546

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 3 of 3

Website: www.saybcrft.com E-mail: saybolt.deerparklab@coreiab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE

45268, Cincinnati	, - ¦

Saybolt

United States

IE USflSATESIES EOHMlX?

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

Submitted date
Sample submitted at
Date received
Date completed
Sample number

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (1050°F plus)

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586594

NAME

METHOD

UNIT

RESULT

Yield on crude
Yield on crude

API Gravity & Relative Density
API Gravity '

Relative Density at 60/60°F 1
Acid Number
Acid number
Sample Size

Inflection or buffer endpoint
Aspbaltenes
Micro Carbon Residue
UOP K factor

Kinematic Viscosity at 210 °F
Kinematic Viscosity at 180 °F
Metals by ICP
Iron (Fe) - Method B
Nickel (Ni) - Method B
Vanadium (V) - Method B
Metals by ICP

Copper (Cu) - Method B
Nitrogen
Sulphur (S)

Elemental analysis
Carbon
Hydrogen
Simulated Distillation
IBP (0.5%)

5% recovered
10% recovered
15% recovered

ASTM D 5236
ASTM D 5236
ASTM D 4052

ASTM D 664

ASTM D 6560
ASTM D 4530
UOP 375
ASTM D 445
ASTM D 445
ASTM D 5708

ASTM D 5708

ASTM D 5762
ASTM D 4294
ASTM D 5291

ASTM D 7169

vol %
mass %

mg KOH/g
9

mass %
mass %

mm2/s
mm2/s

mg/kg
mg/kg
mg/kg

mg/kg
WT%
mass %

mass %
mass %

°F
"F
°F
°F

9.19

11.02

10.5
0.9968

1.1

0.6891

Inf

3.8

19.0

12.0

1294

3423

3.5

38.0

73.4

<0.1

0.5137

1.45

87.13

11.03

976
1038
1062
1079

Alf results in this report refer to the samp!e(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted according to the latest version of the methods, unless another version is specifically indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to me interpretation of test results
as defined in ASTM D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratory.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt Has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536

Tel. +1 281 478 1300 Fax. +1 281 476 9638

Website: www.saybolt.com E-mail: saybolt.deerparKlab@corelab.com

All our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/saybolt/teims-conditions

Print Date: 05-31-2023 15:46
Page 1 of 2

-------
PEGASUS TECHNICAL SERVICES, INC.
26 WEST MARTIN LUTHER KING DRIVE
45268, Cincinnati
United States

Saybolt

Attention of
Analysis Report

Report number

Report Date
Date of issue
Sample object
Sample type
Sample submitted as
Marked

Ms. D. Sundaravadivelu

13072/00015911.1/L/23

Submitted date
Sample submitted at
Date received
Date completed
Sample number

05-31-2023
05-31-2023
Pegasus Tech Svc
Submitted

Petroleum Crude Oil

Bryan Mound Crude Sep 2022 (1050°F plus)

03-24-2023

Saybolt LP, Deer Park

05-07-2023

05-31-2023

14586594

NAME

METHOD

UNIT

RESULT

20% recovered
25% recovered
30% recovered
35% recovered
40% recovered
45% recovered
50% recovered
55% recovered
60% recovered
65% recovered
70% recovered
75% recovered
80% recovered
85% recovered
90% recovered
95% recovered
FBP (99.5%)

Boiling Range Residue

Recovery	

Remarks:

1 By D-70

°F
°F
°F
°F
°F
°F
"F
°F
°F
°F
°F
°F
*F
°F
•F
°F
°F

mass %
mass %

1095
1111
1126
1141
1157
1173
1190
1207
1226
1247
1269
1294
1317

>1328

17.8

82.2

Signed by: Dan Carlson - Chemist III
Issued by: Saybolt LP

Place and date of issue: Deer Park - 05-31-2023

All results in this report refer to the sample(s) tested as taken or submitted like specified in this Analysis report. Uncertainties, available on request, apply in the evaluation of the test results.
All tests are conducted accoiding to the latest version of the methods, unless another version is specifically Indicated. Where available and for convenience purposes, the tested sample has
been checked for compliance with supplied specifications, without accepting any liability for the supplied information. In case of dispute or concern, we refer to the interpretation of test results
as defined in AS I'M D3244, IP 367, ISO 4259 or GOST 33701. This report shall not be partially copied and reproduced without the written permission of the laboratoty.

Saybolt expressly disclaims any representation in this report, whether express or implied, as to the origin of the crude or refined product identified herein. Any designation as to origin is
made solely by the party submitting the sample for analysis. Saybolt has not verified the tested parameters against any supplied specification for purposes of establishing origin. Further,
any certification provided is solely as to the physical and chemical properties of the sample identified in this report.

Saybolt LP 201 Deerwood Glen Dr, Deer Park, TX 77536	Print Date' 05-31-2023 15'46

Tel. +1 281 478 1300 Fax. +1 281 476 9638	Page 2 of 2

Website: www.saybolt.com E-mail: saybolt.deerparklab@corelab.com

Alt our activities are carried out under Saybolt's terms and conditions, available at www.corelab.com/sayboltAerms-conditions

-------
Pegasus Technical Services, Inc.

Bryan Mound Crude Sept. 2022
15911

FRACTIONAL DISTILLATION SUMMARY

LV %

LV%

Cum.LV%

WT%

Cum WT%

API Gravity

SPG @60 F

Original





38.6

0.8320

IBP-75

3.16

3.16

2.23

2.23

111.7

0.5818*

75-175

8.32

11.48

6.76

8.99

79.6

0.6702*

175-250

10.22

21.70

9.14

18.13

60.5

0.7371

250-375

13.87

35.57

13.05

31.18

51.0

0.7755

375-530

15.96

51.53

15.77

46.95

40.6

0.8221

530-650

12.16

63.69

12.50

59.45

34.0

0.8549

650+

36.31

100.00

40.55

100.00

20.8

0.9291

650-850

15.60

79.29

16.77

76.22

26.7

0.8947

850-1050

11.52

90.81

12.76

88.98

22.0

0.9216

1050+

9.19

100.00

11.02

100.00

10.5

0.9968

Total

100.00



100.00







*By GC

The analytical results, opinions or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been
made. The analytical results, opinions or interpretations expressed represent the best judgment of Core Laboratories, however, makes no warranty or representation, express or implied, of
any type, and expressly disclaims same as to the productivity, proper operations or profitableness of any oil, gas, coal or other mineral, property, well or sand in connection with which such report is
used or relied upon for any reason whatsoever. This report shall not be reproduced, in whole or in part, without the written approval of Core Laboratories.

-------
i\

Core Lab

?.£si?.vni?. DPT!?fI2AIIDS

1200 n

1100

1000

900

800

E
E

o

u>

Is"

(§) 700

Li.

d)

q 600
a)

5 500

-------
V

SayboSt

A CDHI LABORATORIES COMPANY

Pegasus
Sample Number
Sample ID

Saybolt LP

A Core Laboratories Company

201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

1S911-14586585

Bryan Mound Crude Sept 2022

Pressure Base, psia
Sample Specific Gravity
Sample Molecular Weight
Sample Density, Lbs/Gallon in Air
Sample Density, Lbs/Gallon in Vacuum
Sample Gross Heating Value, BTU/Lb
Sample Vapor Equivalent, cu .ft/gal
C5 Plus Specific Gravity

14.696
0.5818
59
4.8446
4.8506
21098
31.2
0.7443

5/31/2023
ASTM D2163

Page 1 of 2

IBP-75F

WT %

LV %

MOL %

Ethane

0.21

0.34

0.41

Propane

12.89

14.79

17.25

Isobutane

11.39

11.77

11.57

N-Butane

46 25

46.06

46 9/

2,2-Dimethylpropane

0.25

0.24

0 20

1-Butyne

0.01

0.01

0.01

3-Methyl-1-butene

0.03

0.03

0.03

Isopentane

20.53

19.11

16.80

1-Pentene

0.02

0.02

0.02

N-Pentane

7.49

6.91

6.13

2,2-Dimethylbutane

0.01

0.01

0.01

Cyclopentane

0.05

0.04

0.04

2,3-Dimethylbutane

0.02

0.02

0.01

2-Methylpentane

0.11

0.10

0.08

3-Methylpentane

0.06

0.05

0.04

N-Hexane

0.19

0.17

0.13

Methylcyclopentane

0.05

0.04

0.04

Benzene

0.01

0.01

0.01

Cyclohexane

0.03

0.02

0.02

2-Methylhexane

0.02

0.02

0.01

3-Methylhexane

0.01

0.01

0.01

N-Heptane

0.02

0.02

0.01

Methylcyclohexane

0.02

0.02

0.01

Toluene

0.32

0.21

0.20

Total

100.00

100.02

100.01

Hexanes

0.44

0,39

0.31

Heptanes

0.14

0.12

0.10

Octanes Plus

0.34

0.23

0.21

Tlie analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions,

-------
1

Saybolt

A COSE LABORATORIES COMPANY

Pegasus
Sample Number
Sample ID

15911-14586585

Bryan Mound Crude Sept 2022

IBP-75F

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

C5 Plus Molecular Weight

87.7

C5 Plus Density, Lbs/Gallon in Air

6.1978

C5 Plus Density, Lbs/Gallon in Vacuum

6.2054

C5 Plus Gross Heating Value BTU/Lb

19751

C5 Plus Vapor Equivalent, cu.ft./gal

26.85

C6 Plus Specific Gravity

0.8258

C6 Plus Molecular Weight

91.4

C6 Plus Density, Lbs/Gallon in Air

6 8764

C6 Plus Density, Lbs/Gallon in Vacuum

6.8849

C6 Plus Gross Heating Value BTU/Lb

18878

C6 Plus Vapor Equivalent, cu.ft./gal

28.59

C7 Plus Specific Gravity

0.8659

C7 Plus Molecular Weight

92.5

C7 Plus Density, Lbs/Gallon in Air

7.2103

C7 Plus Density, Lbs/Gallon in Vacuum

7.2192

C7 Plus Gross Heating Value BTU/Lb

18354

C7 Plus Vapor Equivalent, cu.ft./gal

29.62

WT %

LV%

5/31/2023
ASTM D2163
Page 2 of 2
MOL %

The analytical result*, opinions, or interpretations contained in this report art based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims, same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

-------
i

Saybolt

A [DIE LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586586

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

Sample ID

Bryan Mound Crude Sept 2022

4/18/2023
ASTM D-6733
Page 1 of 2

75-175F

WT %

LV %

MOL %

Propane

0.05

0.07

0.09

Isobutane

0.27

0.32

0.37

N-Butane

2.19

2.51

3.00

2,2-Dimethylpropane

0.02

0.02

0.02

1,4-Pentadiene

0.01

0.01

0.01

Isopentane

10.63

11.38

11.75

N-Pentane

22.67

24 06

25.05

2-methyl-1,3-butadiene

0.01

0.01

0.01

3,3-Dimethyl-1 -butene

0.01

0.01

0.01

cis-2-Pentene

0.01

0.01

0.01

2,2-Dimethylbutane

0.47

0.48

0.43

Cyclopentane

2.74

2 44

3.11

2,3-Dimethylbutane

1.30

1.31

1.20

2-Methylpentane

11.67

11.87

10.80

3-Methylpentane

6.98

6.98

6.46

N-Hexane

19.13

19.29

17.70

trans-3-Hexene

0.01

0.01

0.01

2,2-Dimethylpentane

023

0.23

0.18

Methylcyclopentane

8.24

7.32

7.81

2,4-Dimethylpentane

0.53

0.52

0.42

2,2,3-Trimethylbutane

0.04

0.04

0.03

Benzene

2.42

1 83

2.47

3,3-Dimethylpentane

0.07

0.07

0.06

Cyclohexane

5.08

4 34

4.81

2-Methylhexane

1.16

1.14

0.92

2,3-Dimethylpentane

0.41

0.39

0.33

1,1 -Dimethylcyclopentane

0.34

0.30

0.28

3-Methylhexane

0.99

0.96

0.79

cis-1,3-Dimethylcyclopentane

0.43

0.38

0.35

trans-1,3-Dimethylcyclopentane

0.33

0.29

0.27

3-Ethylpentane

0.05

0.05

0.04

trans-1,2-Dimethylcyclopentane

0.58

0.51

0.47

N-Heptane

0.55

0.54

0.44

Methylcyclohexane

0.28

0.24

0.23

2,2-Dimethylhexane

0.01

0.01

0.01

Ethylcyclopentane

0.01

0.01

0.01

The analytical results, opinions, or interpretations contained in this report are based upon information and materia! supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and

-------
. i

Saybolt

A CORE LABORATORIES CDNPANY

Pegasus Tech Svc
Sample Number 15911-14586586
Sample ID	Bryan Mound Crude Sept 2022

75-175F

Toluene

Unidentified

Total

Total Paraffins
Total Isoparaffins
Total Olefins
Total Naphthenes
Total Aromatics
Unclassified
Total C4
Total C5
Total C6
Total C7
Total C8
C4 Paraffin
C5 Paraffin
C6 Paraffin
C7 Paraffin
C4 Isoparaffin
C5 Isoparaffin
C6 Isoparaffin
C7 Isoparaffin
C8 Isoparaffin
C5 Olefin
C6 Olefin
C5 Naphthene
C6 Naphthene
C7 Naphthene
C6 Aromatic
C7 Aromatic

Mol WT of Sample, gm/mol
Density of Sample, gm/cc

Saybolt LP

A Core Laboratories Company

201 Deervvood Glen Dr
Deer Park, TX 77536
281-478-1300

4/18/2023
ASTM D-6733
Page 2 of 2

WT %

LV %

MOL %

0.06

0.05

0.05

0.02

0.00

0.00

100.00

100.00

100.00

44.54

46.40

46.19

34.83

35.77

33.81

0.05

0.05

0.05

18.03

15 83

17.34

2.48

1 88

2 52

0.07

0.07

0.09

246

2.83

3.37

36.10

37.94

39.97

55.30

53.43

51.69

6.06

5.72

4.87

0,01

0.01

0.01

2.19

2.51

3.00

22.67

24.06

25.05

19.13

19.29

17.70

0.55

0.54

0 44

0.27

0.32

0 37

10.65

11.40

11.77

20.42

20.64

18.89

3.48

3.40

2,77

0.01

0.01

0.01

0.04

0.04

0.04

0.01

0.01

0.01

2 74

2.44

3.11

13.32

11.66

12 62

1.97

1.73

1.61

2 42

1.83

247

0.06

0.05

0.05

79.72
0.6695

1 he analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results, opinions, or mterpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their central terms and
conditions,	°

-------
jjtk

.

Saybolt

¦¦IB

Saybolt LP

A Core Laboratories Company
201 Decrwood Glen Dr
Deer Park, TX 77536
281-478-1300

A COM LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586587

5/8/2023
ASTM D-6733

175-250F

WT %

LV %

MOL %

N-Butane

0.02

0 03

0 03

Isopentane

0.03

0.04

0 04

N-Pentane

0 12

0.14

0.17

2,2-Dimethylbutane

0.01

0.01

0.01

Cyclopentane

0.11

0.11

0.16

2,3-Dimethylbutane

0.08

0.09

0.09

2-Methylpentane

0.73

0.82

0.85

3-Methylpentane

0.64

0.71

075

N-Hexane

2.86

3,18

3.34

2,2-Dimethylpentane

0.13

0.14

0.13

Methylcyclopentane

2.36

2.31

2.82

2,4-Dimethylpentane

0.34

0.37

0 34

2,2,3-Trimethylbutane

0.03

0.03

0.03

Benzene

0.75

0.63

0.97

3,3-Dimethylpentane

0.13

0.14

0.13

Cyclohexane

443

4.17

5 29

2-Methylhexane

4.05

4.37

4.07

2,3-Dimethylpentane

1.37

1.44

1.38

1,1 -Dimethylcyclopentane

0.91

0.88

0 93

3-Methylhexane

5.04

5.37

5.06

cis-1,3-Dimethylcyclopentane

2.01

1.98

2.06

trans-1,3-Dimethylcyclopentane

1.91

1.87

1.96

3-Ethylpentane

0.38

0.40

0.38

trans-1,2-Dimethylcyclopentane

3.50

3,41

3.59

2,2,4-Trimethylpentane

0.03

0.03

0.03

N-Heptane

13.80

14.80

13 85

Methylcyclohexane

15.01

14.30

15.38

2,2-Dimethylhexane

1.30

1.37

1.14

Ethylcyclopentane

1.12

1.07

1.15

2,5-Dimethylhexane

0.61

0.64

0.54

2,4-Dimethylhexane

0.80

0.84

0.70

trans,cis-1,2,4-T rimethylcyclopentane

1.26

1.24

1.13

3,3-Dimethylhexane

0.18

0 19

0.16

trans,cis-1,2,3-Trimethylcyclopentane

1.35

1.31

1.21

C8 Olefin

0.39

0.40

0.35

2,3,4-T rimethylpentane

0.15

0.15

0.13

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results, opsmons, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms'and
conditions,	®

-------
\

Saybolt

A CORE LAMRATOHIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14588587

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company

201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 2 of 4

175-250F

WT %

LV %

MOL %

Toluene

6.32

5.35

6.90

2,3-Dimethylhexane

0.57

0.59

0.50

2-Methyl-3-ethylpentane

0.22

022

0.19

2-Ethyl-1-hexene

4.20

4.13

3.76

4-Methylheptane

1.39

1.45

1.22

3,4-Dimethylhexane

0.15

0.15

0.13

cis,trans-1,2,4-T rimethylcyclopentane

0.15

0.14

0.13

3-Methylheptane

3.31

3.44

2.91

cis-1,3-Dimethylcyclohexane

1.90

1.82

1.70

3-Ethylhexane

0.51

0.52

0.45

trans-1,4-Dimethylcyclohexane

0.95

0.91

0.85

1,1 -Dimethylcyclohexane

0.38

0.36

0.34

trans-1-Ethyl-3-methylcyclopentane

0.34

0.33

0.30

cis-1-Ethyl-3-methylcyclopentane

0.35

0.33

0.31

trans-1-Ethyl-2-methylcyclopentane

0.66

0.63

0 59

1 -Ethyl-1 -methylcyclopentane

0.09

0.08

0.08

trans-1,2-Dimethylcyclohexane

1.04

0.98

0.93

cis,cis-1,2,3-T rimethylcyclopentane

0.02

0.02

0.02

trans-3-Octene

0.60

0.61

0.54

cis-1,4-Dimethylcyclohexane

0.05

0.04

0.04

N-Octane

5,47

5.71

4.81

2,2,4-T rimethylhexane

0.04

0.04

0.03

Isopropylcyclopentane

0.04

0.04

0.04

2,3,5-Trimethylhexane

0.03

0.03

0.02

2,2-Dimethylheptane

0.04

0.04

0.03

cis-1,2-Dimethylcyclohexane

0.10

0.09

0.09

2,4-Dimethylheptane

0.14

0.14

0.11

4,4-Dimethylheptane

0.01

0.01

0.01

Ethylcyclohexane

0.85

0.79

0.76

2-Methyl-4-ethylhexane

0.01

0.01

0.01

2,6-Dimethylheptane

0 25

0.26

0.20

1,1,3-T rimethylcyclohexane

0.18

0.17

0.14

C9 Naphthene

0.05

0.05

0.04

2,5-Dimethylheptane

0.11

0.11

0.09

3,5-Dimethylheptane

0.04

0.04

0.03

2-Methyl-3-ethylhexane

0.01

0.01

0.01

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services, Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

-------


Saybolt

A CORE LUflHATOHirS COMPANY

Pegasus Tech Svc

Sample Number 15911-14586587

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 3 of 4

175-250F

WT %

LV %

NIOL %

2,3,3,Trimethylhexane

0.36

0.37

0.28

Ethylbenzene

0.03

003

0.03

2,3,4-Trimethylhexane

0.04

0.04

0.03

trans,trans-1,2,4-T rimethylcyclohexane

0.01

0.01

0.01

Meta-Xylene

0.51

0.43

0.48

Para-Xylene

0.17

0.14

0 16

2,3-Dimethylheptane

0.06

0.06

0.05

3,4-Dimethylheptane D/L

0.04

0.04

0.03

4-Ethylheptane

0.01

0.01

0.01

4-Methyloctane

0.03

0.03

0 02

2-Methyloctane

0.04

0.04

0 03

3-Methyloctane

0.01

0.01

0.01

Styrene

0.03

0.03

0.03

Ortho-Xylene

0.10

0 08

0.09

cis-1-Ethyl-3-methylcyclohexane

0.01

0.01

0.01

N-Nonane

0.02

0.02

0.02

Unidentified

0.03

0.03

0.05

Total

100.00

100.00

100.00

Total Paraffins

22.29

23.88

22.22

Total Isoparaffins

23.45

24.81

22.36

Total Olefins

5.22

5.17

4.68

Total Naphthenes

41.14

39.45

42.06

Total Aromatics

7 88

6.66

863

Unclassified

0.03

0.03

0.05

Total C4

0.02

0.03

0.03

Total C5

0.26

0.29

0.37

Total C6

11.86

11.92

14.12

Total C7

56.05

55.92

57 34

Total C8

30.28

30.30

26.90

Total C9

1.50

1.51

1.19

C4 Paraffin

0.02

0.03

0.03

C5 Paraffin

0.12

0.14

0.17

C6 Paraffin

2.86

3.18

3.34

C7 Paraffin

13.80

14.80

13.85

C8 Paraffin

5.47

5.71

4.81

C9 Paraffin

0.02

0.02

0.02

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

-------
V

Saybolt

A CORE IA10RAT0R1ES COMPANY

Pegasus Tech Svc
Sample Number 15911-14586587
Sample ID

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

Bryan Mound Crude Sept 2022

Mol WT of Sample, gm/mol
Density of Sample, gm/cc

100.59
0.7379

5/8/2023
ASTM D-6733
Page 4 of 4

175-250F

WT %

LV %

MOL %

C5 Isoparaffin

0.03

0.04

0.04

C6 Isoparaffin

1,46

1.63

1.70

C7 Isoparaffin

11.47

12,26

11.52

C8 Isoparaffin

9.26

9.63

8.13

C9 Isoparaffin

1,23

1.25

0.97

C8 Olefin

5,22

5.17

4.68

C5 Naphthene

0.11

0.11

0.16

C6 Naphthene

6.79

6.48

8.11

C7 Naphthene

24.46

23.51

25.07

C8 Naphthene

9.53

9.11

8.52

C9 Naphthene

0.25

0.24

0.20

C6 Aromatic

0.75

0.63

0.97

C7 Aromatic

6.32

5.35

6.90

C8 Aromatic

0.81

068

0.76

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results opinio™, or interpretations expressed represent the best judgement of Saybolt Petroleum Services, Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and

rnnnitmn*	°

-------


5aybolt

A COHI LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281 -478-1300

5/8/2023
ASTM D-6733
Page 1 of 7

250-375F

WT %

LV%

MOL %

N-Hexane

0.01

0.01

0.01

Methylcyclopentane

0.01

0.01

0.02

5-Methyl-1-hexene

0.04

0.04

0.05

2-Methylhexane

0.03

0.03

0.04

2,3-Dimethylpentane

0.01

0.01

0.01

1,1 -Dimethylcyclopentane

0.01

0.01

0.01

3-Methylhexane

0.05

0.06

0.06

5-Methyl-cis-2-hexene

0.03

0.03

0.04

trans-1,3-Dimethylcyclopentane

0.03

0.03

0 04

trans-1,2-Dimethylcyclopentane

0.05

0.05

00/

N-Heptane

0.25

0 28

0.32

2,3-Dimethyl-2-pentene

0.80

0.82

1.04

2,2-Dimethylhexane

0.08

0.09

0.09

Ethylcyclopentane

0.07

0.07

0.09

2,5-Dimethylhexane

0.04

0.04

0.04

2,4-Dimethylhexane

0.06

0.07

0.07

trans, cis-1,2,4-T rimethylcyclopentane

0.14

0.14

0.16

3,3-Dimethylhexane

0.02

0.02

0.02

trans,cis-1,2,3-T rimethylcyclopentane

0.17

0.17

0 19

3-Methyl-1 -heptene

0.02

0.02

0.02

C8 Olefin

0.21

0.22

0.24

Toluene

0.56

0.50

0 78

2,3-Dimethylhexane

0.10

0.11

0.11

2-Methyl-3-ethylpentane

0.04

0.04

0.04

2-Methylheptane

0.97

1.07

1.09

4-Methylheptane

0.34

0.37

038

3,4-Dimethylhexane

0.04

0.04

0.04

cis,trans-1,2,4-T rimethylcyclopentane

0.04

0.04

0.05

3-Methylheptane

0.99

1.08

1.11

cis-1,3-Dimethylcyclohexane

0.92

0.92

1.05

3-Ethylhexane

0.17

0.18

0.19

trans-1,4-Dimethylcyclohexane

0.46

0.46

0.52

1,1 -Dimethylcyclohexane

0.18

0.18

0.21

trans-1-Ethyl-3-methylcyclopentane

0.16

0.16

0.18

cis-1-Ethyl-3-methylcyclopentane

0.17

0.17

0.19

trans-1-Ethyl-2-methylcyclopentane

0.33

0.33

0.38

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

-------
V

Seybolt

A CDHE lABORATMUES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 2 of 7

250-375F

WT %

LV %

MOL %

1 -Ethyl-1 -methylcyclopentane

0.05

0.05

0.06

trans-1,2-Dimethylcyclohexane

0.85

0.84

0.97

cis,cis-1,2,3-Trimethylcyclopentane

0.01

0.01

0.01

trans-3-Octene

0.54

0.58

0.62

cis-1,4-Dimethylcyclohexane

0.03

0.02

0.03

N-Octane

4.45

4.86

4.98

2,2,4-T rimethylhexane

0.08

0.09

0 08

2,3,5-Trimethylhexane

0.07

0.07

0.07

cis-1-Ethyl-2-methylcyclopentane

0.07

0.07

0.08

2,2-Dimethylheptane

0.11

0.12

0.11

cis-1,2-Dimethylcyclohexane

0.23

0.22

026

2,4-Dimethylheptane

0.49

0.53

0.49

4,4-Dimethylheptane

0.06

0.06

0.06

Nonenes

1.48

1.59

1.69

Ethylcyclohexane

2.62

2.55

2 99

2-Methyl-4-ethylhexane

0.04

0.04

0.04

1,1,4-T rimethylcyclohexane

1.20

1.18

1.22

1,1,3-T rimethylcyclohexane

1.47

1.45

1 49

C9 Naphthene

0.87

0.86

0.88

2,5-Dimethylheptane

0.67

0.72

0.67

3,5-Dimethylheptane

0.30

0.32

0.30

2-Methyl-3-ethylhexane

0.13

0.14

0.13

2,3,3,Trimethylhexane

1.07

1.15

1.07

Ethylbenzene

0 35

0.31

0.42

2,3,4-Trimethylhexane

0.88

0.91

0.88

trans,trans-1,2,4-T rimethylcyclohexane

0.07

0.07

0.07

Meta-Xylene

3.02

2 68

3.64

Para-Xylene

0.84

0.75

1.01

2,3-Dimethylheptane

1 09

1.15

1.09

3,4-Dimethylheptane D/L

0.41

0.43

0.41

3,4-Dimethylheptane L/D

0.11

0.12

0.11

4-Ethylheptane

0.06

0.06

0.06

4-Methyloctane

1.15

1.22

1.15

2-Methyloctane

1.47

1.58

1.47

cis, cis-1,2,3-T rimethylcyclohexane

0.11

0.11

0.11

3-Ethylheptane

0.31

0.33

0.31

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
resu is, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and

-------


Saybolt

A CORE LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company

201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733
Page 3 of 7

250-375F

WT%

LV %

NIOL %

3-Methyloctane

0.34

0.36

0.34

trans,cis-1,2,4-T rimethylcyclohexane

1.74

1.71

1.76

Ortho-Xylene

1.82

1.59

2.19

cis,trans-1,2,4-T rimethylcyclohexane

0.10

0.10

0.10

2.4,6-T rimethylheptane

0.04

0.04

0.04

trans-1-Methyl-2-propylcyclopentane

0.02

0.02

0.02

2,2,6-Trimethylheptane

0.98

0 98

0.88

cis-1 -Ethyl-3-methylcyclohexane

0.93

0.89

0.94

trans-1-Ethyl-4-methylcyclohexane

0.73

0.70

0.74

1-Nonene

0.04

0.04

0.04

Isobutylcyclopentane

0.09

0.09

0.09

trans,trans-1,2,3-T rimethylcyclohexane

0.16

0.15

0.16

N-Nonane

8.17

8.73

8.15

1 -Ethyl-1 -methylcyclohexane

0.16

0.16

0.16

trans-1-Ethyl-3-methylcyclohexane

0.12

0.12

0.12

Isopropylbenzene

0.35

0.31

0.37

Bicyclononane

0.84

0.83

0.85

Isopropylcyclohexane

0.49

0.47

0.50

2,4-Dimethyloctane

023

0.25

0.21

2,5-Dimethyloctane

0.21

0.22

0.19

cis-1 -Ethyl-4-methylcyclohexane

0.41

0.40

0.42

Sec-butylcyclopentane

0.07

0.07

0.07

tert-Butylcyclopentane

0.18

0.17

0.18

3,5-Dimethyloctane

1.62

1.70

1.46

N-propylcyclohexane

0.39

0.38

0.40

2,7-Dimethyloctane

0.42

0.45

0.38

2,6-Dimethyloctane

1.64

1.73

1,47

3,3-Dimethyloctane

0.24

0.25

0.22

n-Propylbenzene

0.91

0.81

0.97

3,6-Dimethyloctane

0.36

0.38

0.32

4,5-Dimethyloctane

0.95

0.99

0 85

3-methyl-5-ethylheptane

0.24

0.25

0 22

1 -Methyl-3-ethyl benzene

1.20

1.06

1.28

1 -Methyl-4-ethylbenzene

0.57

0.51

0.61

1,3,5-Trimethylbenzene

0 86

0.76

0 92

4-Ethyloctane

0.39

0.41

0.35

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services, Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms'.nd
conditions.	®

-------
i

Saybolt

A CORE tAMMTOHlES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 4 of 7

250-375F

WT %

LV %

MOL %

2,3-Dimethyloctane

0.19

0.20

0.17

5-Methylnonane

0.08

0.08

0.07

4-Methylnonane

0.37

0.39

0.33

1 -Methyl-2-ethylbenzene

1.65

1.44

1.76

2-Methylnonane

1.18

1.25

1.06

3-Ethyloctane

0.26

0.27

0.23

1,2,3,5-Tetramethylcyclohexane

0.32

0.31

0.29

3-Methylnonane

1.14

1.19

1.02

1,4 Dimethyl-2-ethylcyclohexane

2.18

2.13

1.99

trans-1-Ethyl-2-methylcyclohexane

0.60

0.59

0.61

cis-1-Methyl-3-propylcyclohexane

0.89

0.87

0.81

cis-1,3-Diethylcyclohexane

0.16

0.16

0.15

trans-1,4-Diethylcyclohexane

0.17

0.17

0.16

trans-1-Methyl-3-propylcyclohexane

0.16

0.16

0.15

1 -Ethyl-2,3-dimethylcyclohexane

0.20

0.20

0 18

Decenes

1.66

1.60

1.51

Isobutylbenzene

0.37

0.33

0.35

sec-Butylbenzene

0.44

0.39

0.42

C10 Naphthenes

072

0.70

0.66

C10 Paraffins

0.45

0.46

0.37

N-Decane

5.90

6.20

5.30

trans-1,3-Diethylcyclohexane

0.07

0.07

0.06

1,2,3-T rimethylbenzene

0.87

0.75

0.93

1 -Methyl-3-isopropylbenzene

0.40

0.36

0.38

1 -Methyl-4-isopropylbenzene

0.28

0.25

0.27

cis-1,4-Diethylcyclohexane

0.16

0.16

0.15

Indan (2,3-Dihydroindene)

0.19

0.15

0.21

Sec-butylcyclohexane

0.21

0.21

0.19

1 -Methyl-2-isopropylbenzene

0.56

0.49

0.53

2,6-Dimethylnonane

1.50

1.65

1.23

Butylcyclohexane

1.04

1.00

0.95

1 -Methyl-3-n-propylbenzene

0,55

0.49

0.52

1 -Methyl-4-n-propylbenzene

0.41

0.37

0 39

1,4-Diethylbenzene

0.36

0.32

0.34

N-Butylbenzene

0.32

029

0.30

1,3-Dimethyl-5-ethylbenzene

0.18

0.16

0.17

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Sayboit Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person reiying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

-------
i

Ssyboit

A CORE LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company

201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 5 of 7

250-375F

WT%

LV %

MOL %

1,2-Diethylbenzene

0.08

0.07

0.08

trans-Decalin

0.41

0.35

0.38

1 -Methyl-2-n-propylbenzene

0.56

0.49

0.53

5-Methyldecane

0.44

0.45

0.36

4-Methyldecane

0,48

0.49

0.39

1 »4-Dimethyl-2-ethyl benzene

0.25

0.22

0.24

2-Methyldecane

0.84

0.86

0.69

3-Methyldecane

0.15

0.15

0.12

1,2-Dimethyl-4-ethyl benzene

0.76

0.67

0.72

1,3-Dimethyl-2-ethylbenzene

0.06

0.05

0.06

1,2-Dimethyl-3-Ethylbenzene

0.32

0.28

0.30

C11 Aromatics

0.49

0.43

0.42

C11 Naphthenes

1.22

1.18

0.93

C11 Paraffins

0.30

0.31

0.23

C11 Olefins

0.84

0.75

0.81

N-Undecane

2.60

2.70

2.13

1,2,4,5-Tetramethylbenzene

0,18

0.16

0.17

1,2,3,5-Tetramethylbenzene

0.18

0.16

0.17

C12 Unidentified

0.02

0.02

0.02

5-Methylindan

0.06

0.05

0.06

4-Methylindan

0.13

0.11

0.13

1,2,3,4-Tetramethylbenzene

0.18

0.15

0.17

Pentylbenzene

0.10

0.09

0.09

5-Methylundecane

0.09

0.09

0.07

4-Methylundecane

0.05

0.05

0.04

2-Methylundecane

0.05

0.05

0.04

3-Methylundecane

0.02

0.02

0.02

Tetralin

0.09

0.07

0.09

Naphthalene

0 02

0.02

0.02

1,2-Dimethylindan

0.01

0.01

0.01

2-Ethylindan

0.02

0.02

0.02

1,3,5-Triethylbenzene

0.01

0.01

0.01

N-Dodecane

0 08

0.08

0.06

1,3-Dimethylindan

0,01

0.01

0.01

Unidentified

0.35

0.38

0.25

Total

100.00

100.00

100.00

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made The analytical
results, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or rciied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and

-------
i

Saybolt

k C08E LABDRATDHIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Pound Crude Sept 2022

Saybolt LP

A Core Laboratories Company
201 Deerwood Glen Dr
Deer Park, TX 77536
281-478-1300

5/8/2023
ASTM D-6733

Page 6 of 7

250-375F

WT %

LV %

MOL %

Total Paraffins

21.46

22.86

20.95

Total Isoparaffins

27.05

28.58

25.49

Total Olefins

5.66

5.69

6.06

Total Naphthenes

24.90

24 33

25.17

Total Aromatics

20.76

18.29

22.27

Unclassified

0.18

0.25

0.06

Total C6

0.02

0.02

0.03

Total C7

1.93

1 93

2.55

Total C8

20.60

20.54

23.71

Total C9

35.20

35 39

35.97

Total C10

32.77

32.47

30.00

Total C11

9.00

9.10

7.44

Total C12

0.30

0.30

0.24

C6 Paraffin

0.01

0.01

0.01

C7 Paraffin

0.25

0.28

0.32

C8 Paraffin

4.45

4.86

4.98

C9 Paraffin

8.17

8.73

8.15

C10 Paraffin

5.90

6.20

5.30

C11 Paraffin

2.60

2.70

2.13

C12 Paraffin

0.08

0.08

0.06

C7 Isoparaffin

0.09

0.10

0.11

C8 Isoparaffin

2.93

3.20

326

C9 Isoparaffin

8.76

9.31

8.76

C10 Isoparaffin

11.35

11 85

10.17

C11 Isoparaffin

3.71

3.91

3.02

C12 Isoparaffin

0.21

0.21

0.17

C7 Olefin

0 87

0 89

113

C8 Olefin

0.77

0.82

0.88

C9 Olefin

1.52

1.63

1.73

C10 Olefin

1.66

1.60

1.51

C11 Olefin

0.84

0.75

0.81

C6 Naphthene

0.01

0.01

0.02

C7 Naphthene

0.16

0.16

0.21

C8 Naphthene

6.43

6.33

7.33

C9 Naphthene

10.15

9.93

10.28

C10 Naphthene

6.93

6.72

6.40

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
results opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and

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i

Saybolt

A CUBE LABORATORIES COMPANY

Pegasus Tech Svc

Sample Number 15911-14586588

Sample ID	Bryan Mound Crude Sept 2022

Saybolt LP

A Core Laboratories Company

201 Deerwood Glen Dr

Deer Park, TX 77536
281-478-1300

Mol WT of Sample, gm/mol
Density of Sample, gm/cc

127.89
0.7721

5/8/2023
ASTM D-6733

Page 7 of 7

250-375F

WT %

LV %

MOL %

C11 Naphthene

1.22

1.18

0.93

C7 Aromatic

0.56

0 50

0.78

C8 Aromatic

6.03

5.33

7.26

C9 Aromatic

6.60

5.79

7.05

C10 Aromatic

6.93

6.10

6.62

C11 Aromatic

0,63

0.56

0.55

C12 Aromatic

0.01

0.01

0.01

The analytical results, opinions, or interpretations contained in this report are based upon information and material supplied by the client for whose exclusive and confidential use this report has been made. The analytical
resists, opinions, or interpretations expressed represent the best judgement of Saybolt Petroleum Services. Saybolt Petroleum Services, however, makes no warrant or representation, express or implied, of any type, and
expressly disclaims same with which such report is used or relied upon for any reason whatsoever. Any person relying upon this report should be aware that issuer's activities are carried out under their general terms and
conditions.

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