TSCA Section 5(a)(3)(C) Determination for Microbial Commercial Activity Notice
(MCAN) J-17-0008, 0009, 0010, 0011, 0012, and 0013

Number: J-17-0008, 0009, 0010, 0011, 0012, and 0013

TSCA Section 5(a)(3) Determination: Microorganisms not likely to present an unreasonable
risk (5(a)(3)(C))

Chemical Name:

Generic: Modified microorganism

Assessed Conditions of Use (intended, known, or reasonably foreseen)1:

Intended use(s) (generic): Ethanol Production

Known and reasonably foreseen use(s): Ethanol Production

Summary: The microorganisms are not likely to present an unreasonable risk based on low
human health hazard and low environmental hazard associated with the recipient
microorganisms and introduced genetic material. The introduced genetic modifications pose low
concern for health and environmental hazard and do not include antibiotic resistance markers.

Human Health Hazard2: Human health hazard is relevant to whether a new microorganism is
likely to present an unreasonable risk because the significance of the risk is dependent upon both
the hazard (e.g., pathogenicity/toxicity) of the microorganism and the extent of exposure to the
microorganism. EPA estimated the human health hazard of these microorganisms based on data
for the recipient parental strain as well as the genetic modifications. There is low concern
for human health hazard for the microorganisms based on the recipient strain not being a
human pathogen and the introduced genetic material encoding for common enzymes found
in many microorganisms.

Environmental Hazard3: Environmental hazard is relevant to whether a new microorganism is
likely to present unreasonable risks because the significance of the risk is dependent upon both
the hazard (e.g., pathogenicity/toxicity) of the microorganism and the extent of exposure to the
microorganism. EPA estimated the environmental hazard of these microorganisms based on
data for the recipient parental strain as well as information on the genetic modifications.
There is low concern for environmental hazard for the microorganisms based on the
recipient strain not being an animal or plant pathogen and the introduced genetic material
encoding for common enzymes found in many microorganisms.

1	Intended uses are those identified in the section 5(a) notification. EPA identifies "known" and "reasonably
foreseen" uses of the new microorganism based on evidence of current use of the new microorganism outside the
United States and evidence of the current uses of microorganisms that are structurally analogous to the new
microorganism. EPA identifies uses based on searches of internal CBI EPA MCAN databases (containing use
information on analog microorganisms), other U.S. government public sources, and Internet searches.

2	A microorganism is considered to have low human health hazard if it is not known to be a frank human pathogen
that causes disease in healthy adults, and/or animal studies have demonstrated a lack of pathogenicity or toxicity; a
microorganism is considered to have high human health hazard if there is evidence of adverse effects in humans or
conclusive evidence of severe effects in animal studies. In the absence of animal data on a microorganism, EPA may
use other data or information obtained through literature searches.

3	A microorganism is considered to be of low ecological hazard if it is not known to be an animal or plant pathogen,
and the genetic modifications do not impart pathogenic or toxigenic traits, and the introduced genetic material does
not provide a selective growth advantage in outcompeting indigenous microbial communities in the environment.

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TSCA Section 5(a)(3)(C) Determination for Microbial Commercial Activity Notice
(MCAN) J-17-0008, 0009, 0010, 0011, 0012, and 0013

Potential Exposures: The exposure to a new microorganism is potentially relevant to whether a
new microorganism is likely to present unreasonable risks because the significance of the risk is
dependent upon both the hazard (e.g., pathogenicity/toxicity) of the microorganism and the
nature and extent of exposure to the substance. However, in this case EPA did not estimate the
exposure because EPA determined that the microorganisms present both low human health
hazard and low environmental hazard. Due to low hazard, EPA believes that this microorganism
would be unlikely to present an unreasonable risk even if exposures were high.

Potentially Exposed or Susceptible Subpopulation(s): Workers are potentially exposed.

Given the low hazard of these microorganisms, EPA finds that these microorganisms are not
likely to present unreasonable risk to workers.

7/27/2017	/s/

Date:	Chief, New Chemicals Management Branch,

Chemical Control Division,

Office of Pollution Prevention and Toxics

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